Molecular analyses of the IGS & ITS regions of rDNA of the psychrophilic yeasts in the genus Mrakia View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-01

AUTHORS

Mara R. Diaz, Jack W. Fell

ABSTRACT

Species of the genus Mrakia are currently classified as synonyms based on molecular sequence analyses of the large sub-unit ribosomal DNA (LrDNA). Physiological and protein electrophoretic studies, however, reveal possible species differences. To clarify this discrepancy, we undertook molecular sequence analyses of the internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of rDNA from the four psychrophilic Mrakia species and the psychrophilic yeast, Cryptococcus curiosus. Identical ITS sequences were found between C. curiosus, M. nivalis and M. frigida. Although, M. stokesii and M. gelida displayed identical ITS and IGS sequences, their sequences differed from the other three species by 2.3% and 38%, respectively. The results suggest that M. stokesii is a synonym of M. gelida, whereas M. nivalis is a synonym of M. frigida. Sequence differences (1.9%) observed in the IGS region indicates that C. curiosus is a distinct strain of M. frigida. More... »

PAGES

7-12

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1002048008295

DOI

http://dx.doi.org/10.1023/a:1002048008295

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045859209

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10696872


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