Molecular bases of epithelial cell invasion by Shigella flexneri View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-11

AUTHORS

Philippe J. Sansonetti, Coumaran Egile

ABSTRACT

The pathogenesis of shigellosis is characterized by the capacity of the causative microorganism, Shigella, to invade the epithelial cells that compose the mucosal surface of the colon in humans. The invasive process encompasses several steps which can be summarized as follows: entry of bacteria into epithelial cells involves signalling pathways that elicit a macropinocitic event. Upon contact with the cell surface, S. flexneri activates a Mxi/Spa secretory apparatus encoded by two operons comprising about 25 genes located on a large virulence plasmid of 220 kb. Through this specialized secretory apparatus, Ipa invasins are secreted, two of which (IpaB, 62 kDa and IpaC, 42 kDa) form a complex which is itself able to activate entry via its interaction with the host cell membrane. Interaction of this molecular complex with the cell surface elicits major rearrangements of the host cell cytoskeleton, essentially the polymerization of actin filaments that form bundles supporting the membrane projections which achieve bacterial entry. Active recruitment of the protooncogene pp 60c-src has been demonstrated at the entry site with consequent phosphorylation of cortactin. Also, the small GTPase Rho is controlling the cascade of signals that allows elongation of actin filaments from initial nucleation foci underneath the cell membrane. The regulatory signals involved as well as the proteins recruited indicate that Shigella induces the formation of an adherence plaque at the cell surface in order to achieve entry. Once intracellular, the bacterium lyses its phagocytic vacuole, escapes into the cytoplasm and starts moving the inducing polar, directed polymerization of actin on its surface, due to the expression of IcsA, a 120 kDa outer membrane protein, which is localized at one pole of the microorganism, following cleavage by SopA, a plasmid-encoded surface protease. In the context of polarized epithelial cells, bacteria then reach the intermediate junction and engage their components, particularly the cadherins, to form a protrusion which is actively internalized by the adjacent cell. Bacteria then lyse the two membranes, reach the cytoplasmic compartment again, and resume actin-driven movement. More... »

PAGES

191-197

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1001519806727

DOI

http://dx.doi.org/10.1023/a:1001519806727

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037692059

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10081579


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colon", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dysentery, Bacillary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epithelial Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Intestinal Mucosa", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proto-Oncogene Proteins pp60(c-src)", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rectum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Shigella flexneri", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institut Pasteur", 
          "id": "https://www.grid.ac/institutes/grid.428999.7", 
          "name": [
            "Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, INSERM U 389, Institut Pasteur, 28 rue du Dr Roux, F-75724, Paris C\u00e9dex 15, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sansonetti", 
        "givenName": "Philippe J.", 
        "id": "sg:person.01173563160.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173563160.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur", 
          "id": "https://www.grid.ac/institutes/grid.428999.7", 
          "name": [
            "Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, INSERM U 389, Institut Pasteur, 28 rue du Dr Roux, F-75724, Paris C\u00e9dex 15, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Egile", 
        "givenName": "Coumaran", 
        "id": "sg:person.01331040053.81", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331040053.81"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1992.tb00885.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002337088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.10.3867", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005435628"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.129.2.367", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008067174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(86)90880-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013183780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.3.1254", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014912748"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0169(1997)37:1<44::aid-cm5>3.0.co;2-h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019942998"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0966-842x(96)10039-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020689063"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.183.3.991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020788998"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(94)90358-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022455827"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.36.21878", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030554303"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.185.2.281", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031655356"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0264-410x(91)90128-s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033783102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0264-410x(91)90128-s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033783102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(96)01379-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035553848"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(92)90188-i", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036957097"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.22.10544", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040405275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81070-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040411300"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(94)90260-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041796147"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/364639a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042016511", 
          "https://doi.org/10.1038/364639a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1995.mmi_18030413.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045949963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.14.6572", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048020977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1992.tb01534.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051264476"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/16.10.2717", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052959545"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(96)01295-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053338876"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1674624", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062499461"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.175.18.5899-5906.1993", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062721994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.177.7.1719-1726.1995", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062724622"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076172101", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076915529", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1992.tb05252.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077037148"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1992.tb05253.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077037149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077212204", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079495598", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079677180", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079793595", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081658518", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081688583", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1995.tb07244.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082404875"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1994.tb06863.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082582523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082583376", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082839667", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1996.tb00696.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082902146"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083154813", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1998-11", 
    "datePublishedReg": "1998-11-01", 
    "description": "The pathogenesis of shigellosis is characterized by the capacity of the causative microorganism, Shigella, to invade the epithelial cells that compose the mucosal surface of the colon in humans. The invasive process encompasses several steps which can be summarized as follows: entry of bacteria into epithelial cells involves signalling pathways that elicit a macropinocitic event. Upon contact with the cell surface, S. flexneri activates a Mxi/Spa secretory apparatus encoded by two operons comprising about 25 genes located on a large virulence plasmid of 220 kb. Through this specialized secretory apparatus, Ipa invasins are secreted, two of which (IpaB, 62 kDa and IpaC, 42 kDa) form a complex which is itself able to activate entry via its interaction with the host cell membrane. Interaction of this molecular complex with the cell surface elicits major rearrangements of the host cell cytoskeleton, essentially the polymerization of actin filaments that form bundles supporting the membrane projections which achieve bacterial entry. Active recruitment of the protooncogene pp 60c-src has been demonstrated at the entry site with consequent phosphorylation of cortactin. Also, the small GTPase Rho is controlling the cascade of signals that allows elongation of actin filaments from initial nucleation foci underneath the cell membrane. The regulatory signals involved as well as the proteins recruited indicate that Shigella induces the formation of an adherence plaque at the cell surface in order to achieve entry. Once intracellular, the bacterium lyses its phagocytic vacuole, escapes into the cytoplasm and starts moving the inducing polar, directed polymerization of actin on its surface, due to the expression of IcsA, a 120 kDa outer membrane protein, which is localized at one pole of the microorganism, following cleavage by SopA, a plasmid-encoded surface protease. In the context of polarized epithelial cells, bacteria then reach the intermediate junction and engage their components, particularly the cadherins, to form a protrusion which is actively internalized by the adjacent cell. Bacteria then lyse the two membranes, reach the cytoplasmic compartment again, and resume actin-driven movement.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1023/a:1001519806727", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1017348", 
        "issn": [
          "0003-6072", 
          "1572-9699"
        ], 
        "name": "Antonie van Leeuwenhoek", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "74"
      }
    ], 
    "name": "Molecular bases of epithelial cell invasion by Shigella flexneri", 
    "pagination": "191-197", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b38d66a820c88964b212a710ccaf67eaf322d2df150c677139b376e0aaa73e82"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10081579"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0372625"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1001519806727"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037692059"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1001519806727", 
      "https://app.dimensions.ai/details/publication/pub.1037692059"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:48", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000500.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1023/A:1001519806727"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1001519806727'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1001519806727'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1001519806727'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1001519806727'


 

This table displays all metadata directly associated to this object as RDF triples.

228 TRIPLES      21 PREDICATES      80 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1001519806727 schema:about N0709fdea679a4d1ba08bd4888e565c1f
2 N2488d9cdea314c9ca3cca8ec40746fe8
3 N52db63f2c5c94e148d7f69358d1dd24b
4 N6362c594682b48d9b887947708041e9c
5 Na2386fd82eff448eba7fe0795132ec6e
6 Nc26b9a8cf28d405881137eb5542b0187
7 Ncb6de345e91f4868bbce334b9f6d22ff
8 Ndb09abd3ba5c4587a2e3f741052c66e5
9 Nf030ebc8072e4595aa1130fa1db17957
10 anzsrc-for:06
11 anzsrc-for:0601
12 schema:author N01d15f5760d446299b62df616a9d9180
13 schema:citation sg:pub.10.1038/364639a0
14 https://app.dimensions.ai/details/publication/pub.1076172101
15 https://app.dimensions.ai/details/publication/pub.1076915529
16 https://app.dimensions.ai/details/publication/pub.1077212204
17 https://app.dimensions.ai/details/publication/pub.1079495598
18 https://app.dimensions.ai/details/publication/pub.1079677180
19 https://app.dimensions.ai/details/publication/pub.1079793595
20 https://app.dimensions.ai/details/publication/pub.1081658518
21 https://app.dimensions.ai/details/publication/pub.1081688583
22 https://app.dimensions.ai/details/publication/pub.1082583376
23 https://app.dimensions.ai/details/publication/pub.1082839667
24 https://app.dimensions.ai/details/publication/pub.1083154813
25 https://doi.org/10.1002/(sici)1097-0169(1997)37:1<44::aid-cm5>3.0.co;2-h
26 https://doi.org/10.1002/j.1460-2075.1992.tb05252.x
27 https://doi.org/10.1002/j.1460-2075.1992.tb05253.x
28 https://doi.org/10.1002/j.1460-2075.1994.tb06863.x
29 https://doi.org/10.1002/j.1460-2075.1995.tb07244.x
30 https://doi.org/10.1002/j.1460-2075.1996.tb00696.x
31 https://doi.org/10.1016/0092-8674(86)90880-9
32 https://doi.org/10.1016/0092-8674(92)90188-i
33 https://doi.org/10.1016/0092-8674(94)90260-7
34 https://doi.org/10.1016/0092-8674(94)90358-1
35 https://doi.org/10.1016/0264-410x(91)90128-s
36 https://doi.org/10.1016/0966-842x(96)10039-1
37 https://doi.org/10.1016/s0014-5793(96)01295-1
38 https://doi.org/10.1016/s0014-5793(96)01379-8
39 https://doi.org/10.1016/s0092-8674(00)81070-3
40 https://doi.org/10.1073/pnas.86.10.3867
41 https://doi.org/10.1073/pnas.90.22.10544
42 https://doi.org/10.1073/pnas.92.14.6572
43 https://doi.org/10.1073/pnas.93.3.1254
44 https://doi.org/10.1074/jbc.271.36.21878
45 https://doi.org/10.1083/jcb.129.2.367
46 https://doi.org/10.1084/jem.183.3.991
47 https://doi.org/10.1084/jem.185.2.281
48 https://doi.org/10.1093/emboj/16.10.2717
49 https://doi.org/10.1111/j.1365-2958.1992.tb00885.x
50 https://doi.org/10.1111/j.1365-2958.1992.tb01534.x
51 https://doi.org/10.1111/j.1365-2958.1995.mmi_18030413.x
52 https://doi.org/10.1126/science.1674624
53 https://doi.org/10.1128/jb.175.18.5899-5906.1993
54 https://doi.org/10.1128/jb.177.7.1719-1726.1995
55 schema:datePublished 1998-11
56 schema:datePublishedReg 1998-11-01
57 schema:description The pathogenesis of shigellosis is characterized by the capacity of the causative microorganism, Shigella, to invade the epithelial cells that compose the mucosal surface of the colon in humans. The invasive process encompasses several steps which can be summarized as follows: entry of bacteria into epithelial cells involves signalling pathways that elicit a macropinocitic event. Upon contact with the cell surface, S. flexneri activates a Mxi/Spa secretory apparatus encoded by two operons comprising about 25 genes located on a large virulence plasmid of 220 kb. Through this specialized secretory apparatus, Ipa invasins are secreted, two of which (IpaB, 62 kDa and IpaC, 42 kDa) form a complex which is itself able to activate entry via its interaction with the host cell membrane. Interaction of this molecular complex with the cell surface elicits major rearrangements of the host cell cytoskeleton, essentially the polymerization of actin filaments that form bundles supporting the membrane projections which achieve bacterial entry. Active recruitment of the protooncogene pp 60c-src has been demonstrated at the entry site with consequent phosphorylation of cortactin. Also, the small GTPase Rho is controlling the cascade of signals that allows elongation of actin filaments from initial nucleation foci underneath the cell membrane. The regulatory signals involved as well as the proteins recruited indicate that Shigella induces the formation of an adherence plaque at the cell surface in order to achieve entry. Once intracellular, the bacterium lyses its phagocytic vacuole, escapes into the cytoplasm and starts moving the inducing polar, directed polymerization of actin on its surface, due to the expression of IcsA, a 120 kDa outer membrane protein, which is localized at one pole of the microorganism, following cleavage by SopA, a plasmid-encoded surface protease. In the context of polarized epithelial cells, bacteria then reach the intermediate junction and engage their components, particularly the cadherins, to form a protrusion which is actively internalized by the adjacent cell. Bacteria then lyse the two membranes, reach the cytoplasmic compartment again, and resume actin-driven movement.
58 schema:genre research_article
59 schema:inLanguage en
60 schema:isAccessibleForFree false
61 schema:isPartOf N036d9f8bbfa146bd9c18bfbe5ee0922c
62 N8153bf357da74035b7284f70b3a61ee9
63 sg:journal.1017348
64 schema:name Molecular bases of epithelial cell invasion by Shigella flexneri
65 schema:pagination 191-197
66 schema:productId N2f9e92cb8ca1452190e5e8e311ccd3ef
67 N49efb8be53174c28a0b19a52d9ac52e1
68 N8682ee7a649c4b33b3c19dfc73df5df1
69 Nd95e8fffc2e54ad7906e7295362eea85
70 Ne435abfc6a75407fbcfb3dd3452fc4bc
71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037692059
72 https://doi.org/10.1023/a:1001519806727
73 schema:sdDatePublished 2019-04-10T15:48
74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
75 schema:sdPublisher N91aaa690c2bb4b058280046c1f46548d
76 schema:url http://link.springer.com/10.1023/A:1001519806727
77 sgo:license sg:explorer/license/
78 sgo:sdDataset articles
79 rdf:type schema:ScholarlyArticle
80 N01d15f5760d446299b62df616a9d9180 rdf:first sg:person.01173563160.92
81 rdf:rest N375dd43d51104b8086272de8848c7092
82 N036d9f8bbfa146bd9c18bfbe5ee0922c schema:issueNumber 4
83 rdf:type schema:PublicationIssue
84 N0709fdea679a4d1ba08bd4888e565c1f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Intestinal Mucosa
86 rdf:type schema:DefinedTerm
87 N2488d9cdea314c9ca3cca8ec40746fe8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Bacterial Proteins
89 rdf:type schema:DefinedTerm
90 N2f9e92cb8ca1452190e5e8e311ccd3ef schema:name readcube_id
91 schema:value b38d66a820c88964b212a710ccaf67eaf322d2df150c677139b376e0aaa73e82
92 rdf:type schema:PropertyValue
93 N375dd43d51104b8086272de8848c7092 rdf:first sg:person.01331040053.81
94 rdf:rest rdf:nil
95 N49efb8be53174c28a0b19a52d9ac52e1 schema:name nlm_unique_id
96 schema:value 0372625
97 rdf:type schema:PropertyValue
98 N52db63f2c5c94e148d7f69358d1dd24b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Humans
100 rdf:type schema:DefinedTerm
101 N6362c594682b48d9b887947708041e9c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Proto-Oncogene Proteins pp60(c-src)
103 rdf:type schema:DefinedTerm
104 N8153bf357da74035b7284f70b3a61ee9 schema:volumeNumber 74
105 rdf:type schema:PublicationVolume
106 N8682ee7a649c4b33b3c19dfc73df5df1 schema:name dimensions_id
107 schema:value pub.1037692059
108 rdf:type schema:PropertyValue
109 N91aaa690c2bb4b058280046c1f46548d schema:name Springer Nature - SN SciGraph project
110 rdf:type schema:Organization
111 Na2386fd82eff448eba7fe0795132ec6e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Shigella flexneri
113 rdf:type schema:DefinedTerm
114 Nc26b9a8cf28d405881137eb5542b0187 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Epithelial Cells
116 rdf:type schema:DefinedTerm
117 Ncb6de345e91f4868bbce334b9f6d22ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Dysentery, Bacillary
119 rdf:type schema:DefinedTerm
120 Nd95e8fffc2e54ad7906e7295362eea85 schema:name doi
121 schema:value 10.1023/a:1001519806727
122 rdf:type schema:PropertyValue
123 Ndb09abd3ba5c4587a2e3f741052c66e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Colon
125 rdf:type schema:DefinedTerm
126 Ne435abfc6a75407fbcfb3dd3452fc4bc schema:name pubmed_id
127 schema:value 10081579
128 rdf:type schema:PropertyValue
129 Nf030ebc8072e4595aa1130fa1db17957 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Rectum
131 rdf:type schema:DefinedTerm
132 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
133 schema:name Biological Sciences
134 rdf:type schema:DefinedTerm
135 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
136 schema:name Biochemistry and Cell Biology
137 rdf:type schema:DefinedTerm
138 sg:journal.1017348 schema:issn 0003-6072
139 1572-9699
140 schema:name Antonie van Leeuwenhoek
141 rdf:type schema:Periodical
142 sg:person.01173563160.92 schema:affiliation https://www.grid.ac/institutes/grid.428999.7
143 schema:familyName Sansonetti
144 schema:givenName Philippe J.
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173563160.92
146 rdf:type schema:Person
147 sg:person.01331040053.81 schema:affiliation https://www.grid.ac/institutes/grid.428999.7
148 schema:familyName Egile
149 schema:givenName Coumaran
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331040053.81
151 rdf:type schema:Person
152 sg:pub.10.1038/364639a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042016511
153 https://doi.org/10.1038/364639a0
154 rdf:type schema:CreativeWork
155 https://app.dimensions.ai/details/publication/pub.1076172101 schema:CreativeWork
156 https://app.dimensions.ai/details/publication/pub.1076915529 schema:CreativeWork
157 https://app.dimensions.ai/details/publication/pub.1077212204 schema:CreativeWork
158 https://app.dimensions.ai/details/publication/pub.1079495598 schema:CreativeWork
159 https://app.dimensions.ai/details/publication/pub.1079677180 schema:CreativeWork
160 https://app.dimensions.ai/details/publication/pub.1079793595 schema:CreativeWork
161 https://app.dimensions.ai/details/publication/pub.1081658518 schema:CreativeWork
162 https://app.dimensions.ai/details/publication/pub.1081688583 schema:CreativeWork
163 https://app.dimensions.ai/details/publication/pub.1082583376 schema:CreativeWork
164 https://app.dimensions.ai/details/publication/pub.1082839667 schema:CreativeWork
165 https://app.dimensions.ai/details/publication/pub.1083154813 schema:CreativeWork
166 https://doi.org/10.1002/(sici)1097-0169(1997)37:1<44::aid-cm5>3.0.co;2-h schema:sameAs https://app.dimensions.ai/details/publication/pub.1019942998
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1002/j.1460-2075.1992.tb05252.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077037148
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1002/j.1460-2075.1992.tb05253.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077037149
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1002/j.1460-2075.1994.tb06863.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082582523
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1002/j.1460-2075.1995.tb07244.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082404875
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1002/j.1460-2075.1996.tb00696.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082902146
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1016/0092-8674(86)90880-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013183780
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1016/0092-8674(92)90188-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1036957097
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/0092-8674(94)90260-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041796147
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1016/0092-8674(94)90358-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022455827
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1016/0264-410x(91)90128-s schema:sameAs https://app.dimensions.ai/details/publication/pub.1033783102
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/0966-842x(96)10039-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020689063
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/s0014-5793(96)01295-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053338876
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/s0014-5793(96)01379-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035553848
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/s0092-8674(00)81070-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040411300
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1073/pnas.86.10.3867 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005435628
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1073/pnas.90.22.10544 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040405275
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1073/pnas.92.14.6572 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048020977
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1073/pnas.93.3.1254 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014912748
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1074/jbc.271.36.21878 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030554303
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1083/jcb.129.2.367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008067174
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1084/jem.183.3.991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020788998
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1084/jem.185.2.281 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031655356
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1093/emboj/16.10.2717 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052959545
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1111/j.1365-2958.1992.tb00885.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002337088
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1111/j.1365-2958.1992.tb01534.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051264476
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1111/j.1365-2958.1995.mmi_18030413.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1045949963
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1126/science.1674624 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062499461
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1128/jb.175.18.5899-5906.1993 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062721994
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1128/jb.177.7.1719-1726.1995 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062724622
225 rdf:type schema:CreativeWork
226 https://www.grid.ac/institutes/grid.428999.7 schema:alternateName Institut Pasteur
227 schema:name Unité de Pathogénie Microbienne Moléculaire, INSERM U 389, Institut Pasteur, 28 rue du Dr Roux, F-75724, Paris Cédex 15, France
228 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...