Proteome analysis using selective incorporation of isotopically labeled amino acids View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-01-01

AUTHORS

Timothy D. Veenstra, Suzana Martinović, Gordon A. Anderson, Ljiljana Paša-Tolić, Richard D. Smith

ABSTRACT

A method is described for identifying intact proteins from genomic databases using a combination of accurate molecular mass measurements and partial amino acid content. An initial demonstration was conducted for proteins isolated from Escherichia coli (E. coli) using a multiple auxotrophic strain of K12. Proteins extracted from the organism grown in natural isotopic abundance minimal medium and also minimal medium containing isotopically labeled leucine (Leu-D10), were mixed and analyzed by capillary isoelectric focusing (CIEF) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FTICR). The incorporation of the isotopically labeled Leu residue has no effect on the CIEF separation of the protein, therefore both versions of the protein are observed within the same FTICR spectrum. The difference in the molecular mass of the natural isotopic abundance and Leu-D10 isotopically labeled proteins is used to determine the number of Leu residues present in that particular protein. Knowledge of the molecular mass and number of Leu residues present can be used to unambiguously identify the intact protein. Preliminary results show the efficacy of this method for unambiguously identifying proteins isolated from E. coli. More... »

PAGES

78-82

References to SciGraph publications

Related Patents

  • Increasing The Specificity, Accuracy And Efficiency Of The Assignments Of Particular Proteolytic Peptides By The Incorporation Of Selected Amino Acid Residue(S) Enriched With Stable Isotope(S) Into The Protein Sequence Without The Need For Ultrahigh Instrumental Accuracy
  • Methods For Conducting Metabolic Analyses
  • Molecular Flux Rates Through Critical Pathways Measured By Stable Isotope Labeling In Vivo, As Biomarkers Of Drug Action And Disease Activity
  • Measurement Of Biosynthesis And Breakdown Rates Of Biological Molecules That Are Inaccessible Or Not Easily Accessible To Direct Sampling, Non-Invasively, By Label Incorporation Into Metabolic Derivatives And Catabolitic Products
  • Methods For Measuring Rates Of Reverse Cholesterol Transport In Vivo, As An Index Of Anti-Atherogenesis
  • One Or More 13c And/Or 15n Atoms; Intermediates For Piperazineacetic Acids And Esters, Used For Multiplex Proteomic Analysis.
  • Using Mass Analysis To Monitor Concentration Of Analytes In Fluids; Electrophilic And Chromatographic Separation
  • For The Determination Of An Analyte Or Analytes Such As Proteins, Peptides, Nucleic Acids, Carbohydrates, Lipids, Steroids And Small Molecules By Mass Analysis
  • Sets And Compositions Pertaining To Analyte Determination
  • Method For Replacing Biomarkers Of Protein Kinetics From Tissue Samples By Biomarkers Of Protein Kinetics From Body Fluids After Isotopic Labeling In Vivo
  • Molecular Flux Rates Through Critical Pathways Measured By Stable Isotope Labeling In Vivo, As Biomarkers Of Drug Action And Disease Activity
  • Labeling Reagents; Powerful Tools For Analyte Analysis, Including But Not Limited To Multiplex Proteomic Analysis; Comprises One Or More Heavy Atom Isotopes; Intermediates In The Synthesis Of Active Esters Of N-Substituted Piperazine Acetic Acid
  • Intermediates Of Active Esters For Peptide Synthesis, Labeling, Analysis; Purity
  • Metabolic Flux Measurement, Imaging And Microscopy
  • Method For Automated, Large-Scale Measurement Of The Molecular Flux Rates Of The Proteome Or The Organeome Using Mass Spectrometry
  • Molecular Flux Rates Through Critical Pathways Measured By Stable Isotope Labeling In Vivo, As Biomarkers Of Drug Action And Disease Activity
  • Method For Automated, Large-Scale Measurement Of The Molecular Flux Rates Of The Proteome Or The Organeome Using Mass Spectrometry
  • Active Esters Of N-Substituted Piperazine Acetic Acids, Including Isotopically Enriched Versions Thereof
  • Molecular Flux Rates Through Critical Pathways Measured By Stable Isotope Labeling In Vivo, As Biomarkers Of Drug Action And Disease Activity
  • Using Labeled Substrates To Monitor Efficacy Of Metabolic Pathways; Analyzing Metabolites And Making Correlations Between Metabolite Presence/Concentration And Disease
  • Using Mass Spectroscopy To Identify And Measure Cancer Marker Polypeptide In Bodily Fluids; Tumor Diagnosis
  • Kits Pertaining To Analyte Determination
  • Can Be Used For Drug Screening, Disease Labels, Identifying Drug Targets
  • Molecular Flux Rates Through Critical Pathways Measured By Stable Isotope Labeling In Vivo, As Biomarkers Of Drug Action And Disease Activity
  • Method For Automated, Large-Scale Measurement Of The Molecular Flux Rates Of The Proteome Or The Organeome Using Mass Spectrometry
  • Monitoring Two Dimensions Of Diabetes Pathogenesis
  • Molecular Flux Rates Through Critical Pathways Measured By Stable Isotope Labeling In Vivo, As Biomarkers Of Drug Action And Disease Activity
  • Methods For Identifying The Effect Of A Drug Agent On The Metabolism Of Sugars And Fats In An Individual
  • Method For Automated, Large-Scale Measurement Of The Molecular Flux Rates Of The Proteome Or The Organeome Using Mass Spectrometry
  • Method For High-Throughput Screening Of Compounds And Combinations Of Compounds For Discovery And Quantification Of Actions, Particularly Unanticipated Therapeutic Or Toxic Actions, In Biological Systems
  • Molecular Flux Rates Through Critical Pathways Measured By Stable Isotope Labeling In Vivo, As Biomarkers Of Drug Action And Disease Activity
  • Materials And Methods For Controlling Isotope Effects During Fractionation Of Analytes
  • Measurement Of Biosynthesis And Breakdown Rates Of Biological Molecules That Are Inaccessible Or Not Easily Accessible To Direct Sampling, Non-Invasively, By Label Incorporation Into Metabolic Derivatives And Catabolic Products
  • Method For Automated, Large-Scale Measurement Of The Molecular Flux Rates Of The Proteome Or The Organeome Using Mass Spectrometry
  • Methods For Conducting Metabolic Analyses
  • Methods For Determining The Metabolism Of Sugars And Fats In An Individual
  • Methods For Determining Total Body Skeletal Muscle Mass
  • Molecular Flux Rates Through Critical Pathways Measured By Stable Isotope Labeling In Vivo, As Biomarkers Of Drug Action And Disease Activity
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1016/s1044-0305(99)00120-8

    DOI

    http://dx.doi.org/10.1016/s1044-0305(99)00120-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1003136388

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10631667


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Chemical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0301", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Analytical Chemistry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Electrophoresis, Capillary", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Isoelectric Focusing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Isotope Labeling", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Proteome", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Radioisotopes", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA", 
              "id": "http://www.grid.ac/institutes/grid.436923.9", 
              "name": [
                "Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Veenstra", 
            "givenName": "Timothy D.", 
            "id": "sg:person.011005532437.85", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011005532437.85"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA", 
              "id": "http://www.grid.ac/institutes/grid.436923.9", 
              "name": [
                "Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Martinovi\u0107", 
            "givenName": "Suzana", 
            "id": "sg:person.01106002611.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106002611.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA", 
              "id": "http://www.grid.ac/institutes/grid.436923.9", 
              "name": [
                "Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Anderson", 
            "givenName": "Gordon A.", 
            "id": "sg:person.0603130015.12", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603130015.12"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA", 
              "id": "http://www.grid.ac/institutes/grid.436923.9", 
              "name": [
                "Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pa\u0161a-Toli\u0107", 
            "givenName": "Ljiljana", 
            "id": "sg:person.01365205763.12", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01365205763.12"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA", 
              "id": "http://www.grid.ac/institutes/grid.436923.9", 
              "name": [
                "Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Smith", 
            "givenName": "Richard D.", 
            "id": "sg:person.01132566236.28", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132566236.28"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1016/1044-0305(93)85018-s", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005727158", 
              "https://doi.org/10.1016/1044-0305(93)85018-s"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2000-01-01", 
        "datePublishedReg": "2000-01-01", 
        "description": "A method is described for identifying intact proteins from genomic databases using a combination of accurate molecular mass measurements and partial amino acid content. An initial demonstration was conducted for proteins isolated from Escherichia coli (E. coli) using a multiple auxotrophic strain of K12. Proteins extracted from the organism grown in natural isotopic abundance minimal medium and also minimal medium containing isotopically labeled leucine (Leu-D10), were mixed and analyzed by capillary isoelectric focusing (CIEF) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FTICR). The incorporation of the isotopically labeled Leu residue has no effect on the CIEF separation of the protein, therefore both versions of the protein are observed within the same FTICR spectrum. The difference in the molecular mass of the natural isotopic abundance and Leu-D10 isotopically labeled proteins is used to determine the number of Leu residues present in that particular protein. Knowledge of the molecular mass and number of Leu residues present can be used to unambiguously identify the intact protein. Preliminary results show the efficacy of this method for unambiguously identifying proteins isolated from E. coli.", 
        "genre": "article", 
        "id": "sg:pub.10.1016/s1044-0305(99)00120-8", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1100508", 
            "issn": [
              "1044-0305", 
              "1879-1123"
            ], 
            "name": "Journal of The American Society for Mass Spectrometry", 
            "publisher": "American Chemical Society (ACS)", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "11"
          }
        ], 
        "keywords": [
          "minimal medium", 
          "intact protein", 
          "molecular mass", 
          "Leu residue", 
          "genomic databases", 
          "particular protein", 
          "accurate molecular mass measurements", 
          "auxotrophic strains", 
          "amino acid content", 
          "molecular mass measurements", 
          "Escherichia coli", 
          "amino acids", 
          "protein", 
          "E. coli", 
          "selective incorporation", 
          "coli", 
          "residues", 
          "CIEF separation", 
          "mass spectrometry", 
          "acid content", 
          "organisms", 
          "abundance", 
          "K12", 
          "Fourier transform ion cyclotron resonance mass spectrometry", 
          "leucine", 
          "Leu", 
          "cyclotron resonance mass spectrometry", 
          "ion cyclotron resonance mass spectrometry", 
          "transform ion cyclotron resonance mass spectrometry", 
          "strains", 
          "resonance mass spectrometry", 
          "natural isotopic abundance", 
          "acid", 
          "incorporation", 
          "initial demonstration", 
          "medium", 
          "spectrometry", 
          "number", 
          "mass", 
          "content", 
          "analysis", 
          "number of Leu", 
          "combination", 
          "knowledge", 
          "demonstration", 
          "effect", 
          "differences", 
          "database", 
          "mass measurements", 
          "isotopic abundances", 
          "results", 
          "separation", 
          "method", 
          "spectra", 
          "preliminary results", 
          "efficacy", 
          "version", 
          "measurements"
        ], 
        "name": "Proteome analysis using selective incorporation of isotopically labeled amino acids", 
        "pagination": "78-82", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1003136388"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1016/s1044-0305(99)00120-8"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "10631667"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1016/s1044-0305(99)00120-8", 
          "https://app.dimensions.ai/details/publication/pub.1003136388"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-12-01T06:23", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_343.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1016/s1044-0305(99)00120-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1016/s1044-0305(99)00120-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1016/s1044-0305(99)00120-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1016/s1044-0305(99)00120-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1016/s1044-0305(99)00120-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    179 TRIPLES      21 PREDICATES      91 URIs      82 LITERALS      14 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1016/s1044-0305(99)00120-8 schema:about N2805accee4aa4bd08ca625702ed4e093
    2 N2f0e655c048b4ea083d392c4fceb638e
    3 N3b6ce1b61802420aaca05f22eee442cc
    4 N3f0f96276af04757aad3dc8c7bcaedca
    5 Nd6d581866f1e47d1940230a450fa91a1
    6 Nf9f0fad7dc894707b8fde0b2a9c832f0
    7 Nfd5112ec3d664e16a975744cce023331
    8 anzsrc-for:03
    9 anzsrc-for:0301
    10 schema:author N6b12d3c7c9bb426c9513acdc61a93da7
    11 schema:citation sg:pub.10.1016/1044-0305(93)85018-s
    12 schema:datePublished 2000-01-01
    13 schema:datePublishedReg 2000-01-01
    14 schema:description A method is described for identifying intact proteins from genomic databases using a combination of accurate molecular mass measurements and partial amino acid content. An initial demonstration was conducted for proteins isolated from Escherichia coli (E. coli) using a multiple auxotrophic strain of K12. Proteins extracted from the organism grown in natural isotopic abundance minimal medium and also minimal medium containing isotopically labeled leucine (Leu-D10), were mixed and analyzed by capillary isoelectric focusing (CIEF) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FTICR). The incorporation of the isotopically labeled Leu residue has no effect on the CIEF separation of the protein, therefore both versions of the protein are observed within the same FTICR spectrum. The difference in the molecular mass of the natural isotopic abundance and Leu-D10 isotopically labeled proteins is used to determine the number of Leu residues present in that particular protein. Knowledge of the molecular mass and number of Leu residues present can be used to unambiguously identify the intact protein. Preliminary results show the efficacy of this method for unambiguously identifying proteins isolated from E. coli.
    15 schema:genre article
    16 schema:isAccessibleForFree false
    17 schema:isPartOf N137f19daa5834d13a164f453791d2349
    18 Na61be87e2625489787f594226c5d4216
    19 sg:journal.1100508
    20 schema:keywords CIEF separation
    21 E. coli
    22 Escherichia coli
    23 Fourier transform ion cyclotron resonance mass spectrometry
    24 K12
    25 Leu
    26 Leu residue
    27 abundance
    28 accurate molecular mass measurements
    29 acid
    30 acid content
    31 amino acid content
    32 amino acids
    33 analysis
    34 auxotrophic strains
    35 coli
    36 combination
    37 content
    38 cyclotron resonance mass spectrometry
    39 database
    40 demonstration
    41 differences
    42 effect
    43 efficacy
    44 genomic databases
    45 incorporation
    46 initial demonstration
    47 intact protein
    48 ion cyclotron resonance mass spectrometry
    49 isotopic abundances
    50 knowledge
    51 leucine
    52 mass
    53 mass measurements
    54 mass spectrometry
    55 measurements
    56 medium
    57 method
    58 minimal medium
    59 molecular mass
    60 molecular mass measurements
    61 natural isotopic abundance
    62 number
    63 number of Leu
    64 organisms
    65 particular protein
    66 preliminary results
    67 protein
    68 residues
    69 resonance mass spectrometry
    70 results
    71 selective incorporation
    72 separation
    73 spectra
    74 spectrometry
    75 strains
    76 transform ion cyclotron resonance mass spectrometry
    77 version
    78 schema:name Proteome analysis using selective incorporation of isotopically labeled amino acids
    79 schema:pagination 78-82
    80 schema:productId N2221795f68674cfd858bb87cf0d3b961
    81 N7a86674c98a44666aee9afb849cbcaa9
    82 Nb0a89438d80e41249ce0afce064c6148
    83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003136388
    84 https://doi.org/10.1016/s1044-0305(99)00120-8
    85 schema:sdDatePublished 2022-12-01T06:23
    86 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    87 schema:sdPublisher N31a603f915534ca8b246f1596946cbb4
    88 schema:url https://doi.org/10.1016/s1044-0305(99)00120-8
    89 sgo:license sg:explorer/license/
    90 sgo:sdDataset articles
    91 rdf:type schema:ScholarlyArticle
    92 N040a620f69194228949fdc10630fe622 rdf:first sg:person.01106002611.63
    93 rdf:rest N1b0808213a6d4e84835742eaf06cb0fa
    94 N137f19daa5834d13a164f453791d2349 schema:volumeNumber 11
    95 rdf:type schema:PublicationVolume
    96 N1b0808213a6d4e84835742eaf06cb0fa rdf:first sg:person.0603130015.12
    97 rdf:rest N669a2456e86f4f62b71c05bbe5f8ba41
    98 N2221795f68674cfd858bb87cf0d3b961 schema:name pubmed_id
    99 schema:value 10631667
    100 rdf:type schema:PropertyValue
    101 N2805accee4aa4bd08ca625702ed4e093 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Isoelectric Focusing
    103 rdf:type schema:DefinedTerm
    104 N2f0e655c048b4ea083d392c4fceb638e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Radioisotopes
    106 rdf:type schema:DefinedTerm
    107 N31a603f915534ca8b246f1596946cbb4 schema:name Springer Nature - SN SciGraph project
    108 rdf:type schema:Organization
    109 N3b6ce1b61802420aaca05f22eee442cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Escherichia coli
    111 rdf:type schema:DefinedTerm
    112 N3f0f96276af04757aad3dc8c7bcaedca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Amino Acids
    114 rdf:type schema:DefinedTerm
    115 N669a2456e86f4f62b71c05bbe5f8ba41 rdf:first sg:person.01365205763.12
    116 rdf:rest N9d097fc825994f45957a55441c7e2102
    117 N6b12d3c7c9bb426c9513acdc61a93da7 rdf:first sg:person.011005532437.85
    118 rdf:rest N040a620f69194228949fdc10630fe622
    119 N7a86674c98a44666aee9afb849cbcaa9 schema:name doi
    120 schema:value 10.1016/s1044-0305(99)00120-8
    121 rdf:type schema:PropertyValue
    122 N9d097fc825994f45957a55441c7e2102 rdf:first sg:person.01132566236.28
    123 rdf:rest rdf:nil
    124 Na61be87e2625489787f594226c5d4216 schema:issueNumber 1
    125 rdf:type schema:PublicationIssue
    126 Nb0a89438d80e41249ce0afce064c6148 schema:name dimensions_id
    127 schema:value pub.1003136388
    128 rdf:type schema:PropertyValue
    129 Nd6d581866f1e47d1940230a450fa91a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Electrophoresis, Capillary
    131 rdf:type schema:DefinedTerm
    132 Nf9f0fad7dc894707b8fde0b2a9c832f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Proteome
    134 rdf:type schema:DefinedTerm
    135 Nfd5112ec3d664e16a975744cce023331 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    136 schema:name Isotope Labeling
    137 rdf:type schema:DefinedTerm
    138 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
    139 schema:name Chemical Sciences
    140 rdf:type schema:DefinedTerm
    141 anzsrc-for:0301 schema:inDefinedTermSet anzsrc-for:
    142 schema:name Analytical Chemistry
    143 rdf:type schema:DefinedTerm
    144 sg:journal.1100508 schema:issn 1044-0305
    145 1879-1123
    146 schema:name Journal of The American Society for Mass Spectrometry
    147 schema:publisher American Chemical Society (ACS)
    148 rdf:type schema:Periodical
    149 sg:person.011005532437.85 schema:affiliation grid-institutes:grid.436923.9
    150 schema:familyName Veenstra
    151 schema:givenName Timothy D.
    152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011005532437.85
    153 rdf:type schema:Person
    154 sg:person.01106002611.63 schema:affiliation grid-institutes:grid.436923.9
    155 schema:familyName Martinović
    156 schema:givenName Suzana
    157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106002611.63
    158 rdf:type schema:Person
    159 sg:person.01132566236.28 schema:affiliation grid-institutes:grid.436923.9
    160 schema:familyName Smith
    161 schema:givenName Richard D.
    162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132566236.28
    163 rdf:type schema:Person
    164 sg:person.01365205763.12 schema:affiliation grid-institutes:grid.436923.9
    165 schema:familyName Paša-Tolić
    166 schema:givenName Ljiljana
    167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01365205763.12
    168 rdf:type schema:Person
    169 sg:person.0603130015.12 schema:affiliation grid-institutes:grid.436923.9
    170 schema:familyName Anderson
    171 schema:givenName Gordon A.
    172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603130015.12
    173 rdf:type schema:Person
    174 sg:pub.10.1016/1044-0305(93)85018-s schema:sameAs https://app.dimensions.ai/details/publication/pub.1005727158
    175 https://doi.org/10.1016/1044-0305(93)85018-s
    176 rdf:type schema:CreativeWork
    177 grid-institutes:grid.436923.9 schema:alternateName Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA
    178 schema:name Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA
    179 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...