Mass-tag technology responding to intracellular signals as a novel assay system for the diagnosis of tumor View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2007-01

AUTHORS

Jeong -Hun Kang, Yoshiki Katayama, Aishan Han, Shuhei Shigaki, Jun Oishi, Kenji Kawamura, Riki Toita, Xiao Ming Han, Takeshi Mori, Takuro Niidome

ABSTRACT

A novel mass spectrometry-based assay system for determining protein kinase activity employing mass-tagged substrate peptide probes was used for the diagnosis of tumors. Two peptide probes (H-type and D-type) were synthesized containing the same substrate peptide sequence for protein kinase C (PKC). The molecular weights of the two probes differ because of the incorporation of deuterium into the acetyl groups of the D-type probe. The lysates of the normal and tumor tissue were prepared and reacted with the H- and D-type peptide probes, respectively. The PKC activities of the normal and tumor tissues can be compared simply and directly by calculating the phosphorylated ratio to each peptide probe, obtained from the peak intensity of the mass spectrum after mixing of the two reaction solutions. The phosphorylation ratio for the reaction of the H-type peptide probe with the tumor tissue lysate (B16 melanoma) was more than three times higher than that of the D type peptide probe with the normal skin tissue lysate. These results show that the novel assay system for detecting protein kinase activity using mass-tag technology can be a simple and useful means to profile protein kinase activity for cell or tissue lysate samples, and can be applied to the diagnosis of tumors. More... »

PAGES

106-112

References to SciGraph publications

  • 2005-06. Protein kinase C in melanoma in CANCER AND METASTASIS REVIEWS
  • 2005-04. Optimization of a MALDI TOF-TOF mass spectrometer for intact protein analysis in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 2006-06. Simultaneous Analysis of Phosphorylated Peptides by MALDI-TOF-MS in CHROMATOGRAPHIA
  • 2005-12. New and automated MSn approaches for top-down identification of modified proteins in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 2005-06. MALDI-TOF MS analysis of urinary nucleosides in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1016/j.jasms.2006.09.004

    DOI

    http://dx.doi.org/10.1016/j.jasms.2006.09.004

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1010437314

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17046276


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line, Tumor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Enzyme Inhibitors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Indoles", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Male", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Maleimides", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Melanoma, Experimental", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, Inbred Strains", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neoplasm Transplantation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Peptides", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phosphorylation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Kinase C", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Kinases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Skin", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Skin Neoplasms", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Japan Science and Technology Agency", 
              "id": "https://www.grid.ac/institutes/grid.419082.6", 
              "name": [
                "CREST, Japan Science and Technology Corporation, Kawaguchi, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kang", 
            "givenName": "Jeong -Hun", 
            "id": "sg:person.0760576056.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760576056.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Kyushu University", 
              "id": "https://www.grid.ac/institutes/grid.177174.3", 
              "name": [
                "CREST, Japan Science and Technology Corporation, Kawaguchi, Japan", 
                "Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, 819-0395, Fukuoka, Japan", 
                "Center for Future Chemistry, Kyushu University, Fukuoka, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Katayama", 
            "givenName": "Yoshiki", 
            "id": "sg:person.01217553115.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01217553115.49"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "RIKEN", 
              "id": "https://www.grid.ac/institutes/grid.7597.c", 
              "name": [
                "Riken, Wako, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Han", 
            "givenName": "Aishan", 
            "id": "sg:person.01123243142.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123243142.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Kyushu University", 
              "id": "https://www.grid.ac/institutes/grid.177174.3", 
              "name": [
                "Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, 819-0395, Fukuoka, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shigaki", 
            "givenName": "Shuhei", 
            "id": "sg:person.01324313543.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324313543.40"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Kyushu University", 
              "id": "https://www.grid.ac/institutes/grid.177174.3", 
              "name": [
                "Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, 819-0395, Fukuoka, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Oishi", 
            "givenName": "Jun", 
            "id": "sg:person.01174667253.69", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174667253.69"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Kyushu University", 
              "id": "https://www.grid.ac/institutes/grid.177174.3", 
              "name": [
                "Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, 819-0395, Fukuoka, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kawamura", 
            "givenName": "Kenji", 
            "id": "sg:person.01240026312.83", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240026312.83"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Kyushu University", 
              "id": "https://www.grid.ac/institutes/grid.177174.3", 
              "name": [
                "Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, 819-0395, Fukuoka, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Toita", 
            "givenName": "Riki", 
            "id": "sg:person.01105566503.21", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105566503.21"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Kyushu University", 
              "id": "https://www.grid.ac/institutes/grid.177174.3", 
              "name": [
                "Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, 819-0395, Fukuoka, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Han", 
            "givenName": "Xiao Ming", 
            "id": "sg:person.0656131017.90", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656131017.90"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Kyushu University", 
              "id": "https://www.grid.ac/institutes/grid.177174.3", 
              "name": [
                "Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, 819-0395, Fukuoka, Japan", 
                "Center for Future Chemistry, Kyushu University, Fukuoka, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mori", 
            "givenName": "Takeshi", 
            "id": "sg:person.01266160666.47", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266160666.47"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Kyushu University", 
              "id": "https://www.grid.ac/institutes/grid.177174.3", 
              "name": [
                "Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, 819-0395, Fukuoka, Japan", 
                "Center for Future Chemistry, Kyushu University, Fukuoka, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Niidome", 
            "givenName": "Takuro", 
            "id": "sg:person.0646211241.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646211241.31"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1016/j.jasms.2004.12.018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000219791", 
              "https://doi.org/10.1016/j.jasms.2004.12.018"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1677/erc.0.0100389", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001145868"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1677/erc.0.0100389", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001145868"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj2940335", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005284465"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj2940335", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005284465"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bmcl.2003.12.022", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007173983"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj2830159", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007339140"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj2830159", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007339140"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbapap.2003.11.012", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008331220"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0955-0674(97)80058-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014146189"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1016/j.jasms.2005.02.018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014903416", 
              "https://doi.org/10.1016/j.jasms.2005.02.018"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1016/j.jasms.2005.08.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015022515", 
              "https://doi.org/10.1016/j.jasms.2005.08.004"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10555-005-1578-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015867464", 
              "https://doi.org/10.1007/s10555-005-1578-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0955-0674(90)90006-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022246267"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ab.2005.02.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023665894"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1365/s10337-006-0810-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028813380", 
              "https://doi.org/10.1365/s10337-006-0810-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1365/s10337-006-0810-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028813380", 
              "https://doi.org/10.1365/s10337-006-0810-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20020786", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030698407"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20020786", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030698407"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00008390-200404000-00002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031557583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00008390-200404000-00002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031557583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jconrel.2005.10.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035015382"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2116/analsci.17.277", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045016300"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj2910329", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051994416"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj2910329", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051994416"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ac050135t", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1054996837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ac050135t", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1054996837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ac051519m", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1054997464"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ac051519m", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1054997464"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bm025532h", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055219708"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bm025532h", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055219708"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1615/critrevoncog.v5.i5.10", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1068132616"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2007-01", 
        "datePublishedReg": "2007-01-01", 
        "description": "A novel mass spectrometry-based assay system for determining protein kinase activity employing mass-tagged substrate peptide probes was used for the diagnosis of tumors. Two peptide probes (H-type and D-type) were synthesized containing the same substrate peptide sequence for protein kinase C (PKC). The molecular weights of the two probes differ because of the incorporation of deuterium into the acetyl groups of the D-type probe. The lysates of the normal and tumor tissue were prepared and reacted with the H- and D-type peptide probes, respectively. The PKC activities of the normal and tumor tissues can be compared simply and directly by calculating the phosphorylated ratio to each peptide probe, obtained from the peak intensity of the mass spectrum after mixing of the two reaction solutions. The phosphorylation ratio for the reaction of the H-type peptide probe with the tumor tissue lysate (B16 melanoma) was more than three times higher than that of the D type peptide probe with the normal skin tissue lysate. These results show that the novel assay system for detecting protein kinase activity using mass-tag technology can be a simple and useful means to profile protein kinase activity for cell or tissue lysate samples, and can be applied to the diagnosis of tumors.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1016/j.jasms.2006.09.004", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1100508", 
            "issn": [
              "1044-0305", 
              "1879-1123"
            ], 
            "name": "Journal of The American Society for Mass Spectrometry", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "18"
          }
        ], 
        "name": "Mass-tag technology responding to intracellular signals as a novel assay system for the diagnosis of tumor", 
        "pagination": "106-112", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "31b93d4bbc5a6c4bfc6ce3c80d3ce2e3ae409c76d249a4e803c5980e6ba7a734"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "17046276"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9010412"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1016/j.jasms.2006.09.004"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1010437314"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1016/j.jasms.2006.09.004", 
          "https://app.dimensions.ai/details/publication/pub.1010437314"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T14:57", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000498.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1016/j.jasms.2006.09.004"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1016/j.jasms.2006.09.004'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1016/j.jasms.2006.09.004'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1016/j.jasms.2006.09.004'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1016/j.jasms.2006.09.004'


     

    This table displays all metadata directly associated to this object as RDF triples.

    279 TRIPLES      21 PREDICATES      68 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1016/j.jasms.2006.09.004 schema:about N0c535d6bda634423a7ce406a5b4cf024
    2 N0e18c7ee0b8a4b038c793b7ff015b149
    3 N13eeb14a69914137bc159c7760aa600f
    4 N1afb8bace62e4ef1b9c68f665a022ae0
    5 N2503ea26e3fb4cd2a5af36462cce4a94
    6 N3612da43bef84221a8f6fdca5aa90529
    7 N4dc6e96e747848be8d07d873f36e8f11
    8 N5307c891b48f43d3b67bb82b473c234f
    9 N6f77bdd4bbbe4d11b4cd94b93e54ce53
    10 N7ce87953d2674c8e91741aa5c4a45d9c
    11 N840949d2347944be9c90259c90f862e0
    12 N870a24a6554741129c2cc9bab3da6810
    13 N8759139e4d544285b646aef4a6c80094
    14 N898ac70a952d4a6bb7a742e23fe9f8c4
    15 Nbc37aa2e950a44fcbb9ffb005ad03ffa
    16 Nbf23b23b6e934d3cab1293d9b291c4aa
    17 Ne8cdb63ff14240258b29e5450eba9e40
    18 anzsrc-for:06
    19 anzsrc-for:0601
    20 schema:author Nb270225b9fe7466f9a4a4532ee8a71ed
    21 schema:citation sg:pub.10.1007/s10555-005-1578-8
    22 sg:pub.10.1016/j.jasms.2004.12.018
    23 sg:pub.10.1016/j.jasms.2005.02.018
    24 sg:pub.10.1016/j.jasms.2005.08.004
    25 sg:pub.10.1365/s10337-006-0810-1
    26 https://doi.org/10.1016/0955-0674(90)90006-z
    27 https://doi.org/10.1016/j.ab.2005.02.008
    28 https://doi.org/10.1016/j.bbapap.2003.11.012
    29 https://doi.org/10.1016/j.bmcl.2003.12.022
    30 https://doi.org/10.1016/j.jconrel.2005.10.007
    31 https://doi.org/10.1016/s0955-0674(97)80058-0
    32 https://doi.org/10.1021/ac050135t
    33 https://doi.org/10.1021/ac051519m
    34 https://doi.org/10.1021/bm025532h
    35 https://doi.org/10.1042/bj20020786
    36 https://doi.org/10.1042/bj2830159
    37 https://doi.org/10.1042/bj2910329
    38 https://doi.org/10.1042/bj2940335
    39 https://doi.org/10.1097/00008390-200404000-00002
    40 https://doi.org/10.1615/critrevoncog.v5.i5.10
    41 https://doi.org/10.1677/erc.0.0100389
    42 https://doi.org/10.2116/analsci.17.277
    43 schema:datePublished 2007-01
    44 schema:datePublishedReg 2007-01-01
    45 schema:description A novel mass spectrometry-based assay system for determining protein kinase activity employing mass-tagged substrate peptide probes was used for the diagnosis of tumors. Two peptide probes (H-type and D-type) were synthesized containing the same substrate peptide sequence for protein kinase C (PKC). The molecular weights of the two probes differ because of the incorporation of deuterium into the acetyl groups of the D-type probe. The lysates of the normal and tumor tissue were prepared and reacted with the H- and D-type peptide probes, respectively. The PKC activities of the normal and tumor tissues can be compared simply and directly by calculating the phosphorylated ratio to each peptide probe, obtained from the peak intensity of the mass spectrum after mixing of the two reaction solutions. The phosphorylation ratio for the reaction of the H-type peptide probe with the tumor tissue lysate (B16 melanoma) was more than three times higher than that of the D type peptide probe with the normal skin tissue lysate. These results show that the novel assay system for detecting protein kinase activity using mass-tag technology can be a simple and useful means to profile protein kinase activity for cell or tissue lysate samples, and can be applied to the diagnosis of tumors.
    46 schema:genre research_article
    47 schema:inLanguage en
    48 schema:isAccessibleForFree true
    49 schema:isPartOf N79a4a9eb244f4dc986c5284d14fb7032
    50 N8408a7bc543642048cc32c6daf089b09
    51 sg:journal.1100508
    52 schema:name Mass-tag technology responding to intracellular signals as a novel assay system for the diagnosis of tumor
    53 schema:pagination 106-112
    54 schema:productId N1e98b7b7ad1c4c4ab7484265edb10148
    55 N21ae609096814fd1b824f51e0182cd4f
    56 N631a636ee570449f8d4abf7f94ea6f5d
    57 N7bdbd73521e64d23841834b0e71435a8
    58 Nf3cdb2699c284fb79c29eb3ad10b3b23
    59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010437314
    60 https://doi.org/10.1016/j.jasms.2006.09.004
    61 schema:sdDatePublished 2019-04-10T14:57
    62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    63 schema:sdPublisher N75d19191725f432eb0f35fca10bd61f8
    64 schema:url http://link.springer.com/10.1016/j.jasms.2006.09.004
    65 sgo:license sg:explorer/license/
    66 sgo:sdDataset articles
    67 rdf:type schema:ScholarlyArticle
    68 N0c535d6bda634423a7ce406a5b4cf024 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    69 schema:name Peptides
    70 rdf:type schema:DefinedTerm
    71 N0e18c7ee0b8a4b038c793b7ff015b149 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    72 schema:name Indoles
    73 rdf:type schema:DefinedTerm
    74 N13eeb14a69914137bc159c7760aa600f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    75 schema:name Enzyme Inhibitors
    76 rdf:type schema:DefinedTerm
    77 N1afb8bace62e4ef1b9c68f665a022ae0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    78 schema:name Protein Kinase C
    79 rdf:type schema:DefinedTerm
    80 N1e98b7b7ad1c4c4ab7484265edb10148 schema:name dimensions_id
    81 schema:value pub.1010437314
    82 rdf:type schema:PropertyValue
    83 N21ae609096814fd1b824f51e0182cd4f schema:name nlm_unique_id
    84 schema:value 9010412
    85 rdf:type schema:PropertyValue
    86 N2503ea26e3fb4cd2a5af36462cce4a94 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    87 schema:name Mice
    88 rdf:type schema:DefinedTerm
    89 N3318c00f25e0457aa57ace74321e8d7a rdf:first sg:person.0656131017.90
    90 rdf:rest N695201378dcf4cb2bc806a136810ec59
    91 N3612da43bef84221a8f6fdca5aa90529 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name Male
    93 rdf:type schema:DefinedTerm
    94 N397e6972407e4779b9f9d7511783d88d rdf:first sg:person.01240026312.83
    95 rdf:rest Nfc5b383da02046cba819f3d27dfa5630
    96 N4dc6e96e747848be8d07d873f36e8f11 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Phosphorylation
    98 rdf:type schema:DefinedTerm
    99 N5307c891b48f43d3b67bb82b473c234f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Neoplasm Transplantation
    101 rdf:type schema:DefinedTerm
    102 N61e757ca7a5e4ddebe3dc0531d399182 rdf:first sg:person.01324313543.40
    103 rdf:rest Ncefeeeea82744656a6ca4172bf04c379
    104 N631a636ee570449f8d4abf7f94ea6f5d schema:name pubmed_id
    105 schema:value 17046276
    106 rdf:type schema:PropertyValue
    107 N695201378dcf4cb2bc806a136810ec59 rdf:first sg:person.01266160666.47
    108 rdf:rest N8968fdd03f084ef79cab02d612d54013
    109 N6f77bdd4bbbe4d11b4cd94b93e54ce53 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Animals
    111 rdf:type schema:DefinedTerm
    112 N75d19191725f432eb0f35fca10bd61f8 schema:name Springer Nature - SN SciGraph project
    113 rdf:type schema:Organization
    114 N79a4a9eb244f4dc986c5284d14fb7032 schema:volumeNumber 18
    115 rdf:type schema:PublicationVolume
    116 N7bdbd73521e64d23841834b0e71435a8 schema:name doi
    117 schema:value 10.1016/j.jasms.2006.09.004
    118 rdf:type schema:PropertyValue
    119 N7ce87953d2674c8e91741aa5c4a45d9c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Melanoma, Experimental
    121 rdf:type schema:DefinedTerm
    122 N831f34a44a1042d991cc8b8698a1545b rdf:first sg:person.01217553115.49
    123 rdf:rest Nd5925f81dc874eca84f866cdec8ba2d4
    124 N8408a7bc543642048cc32c6daf089b09 schema:issueNumber 1
    125 rdf:type schema:PublicationIssue
    126 N840949d2347944be9c90259c90f862e0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
    128 rdf:type schema:DefinedTerm
    129 N870a24a6554741129c2cc9bab3da6810 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Skin Neoplasms
    131 rdf:type schema:DefinedTerm
    132 N8759139e4d544285b646aef4a6c80094 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Protein Kinases
    134 rdf:type schema:DefinedTerm
    135 N8968fdd03f084ef79cab02d612d54013 rdf:first sg:person.0646211241.31
    136 rdf:rest rdf:nil
    137 N898ac70a952d4a6bb7a742e23fe9f8c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Maleimides
    139 rdf:type schema:DefinedTerm
    140 Nb270225b9fe7466f9a4a4532ee8a71ed rdf:first sg:person.0760576056.02
    141 rdf:rest N831f34a44a1042d991cc8b8698a1545b
    142 Nbc37aa2e950a44fcbb9ffb005ad03ffa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Mice, Inbred Strains
    144 rdf:type schema:DefinedTerm
    145 Nbf23b23b6e934d3cab1293d9b291c4aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    146 schema:name Skin
    147 rdf:type schema:DefinedTerm
    148 Ncefeeeea82744656a6ca4172bf04c379 rdf:first sg:person.01174667253.69
    149 rdf:rest N397e6972407e4779b9f9d7511783d88d
    150 Nd5925f81dc874eca84f866cdec8ba2d4 rdf:first sg:person.01123243142.22
    151 rdf:rest N61e757ca7a5e4ddebe3dc0531d399182
    152 Ne8cdb63ff14240258b29e5450eba9e40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Cell Line, Tumor
    154 rdf:type schema:DefinedTerm
    155 Nf3cdb2699c284fb79c29eb3ad10b3b23 schema:name readcube_id
    156 schema:value 31b93d4bbc5a6c4bfc6ce3c80d3ce2e3ae409c76d249a4e803c5980e6ba7a734
    157 rdf:type schema:PropertyValue
    158 Nfc5b383da02046cba819f3d27dfa5630 rdf:first sg:person.01105566503.21
    159 rdf:rest N3318c00f25e0457aa57ace74321e8d7a
    160 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    161 schema:name Biological Sciences
    162 rdf:type schema:DefinedTerm
    163 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    164 schema:name Biochemistry and Cell Biology
    165 rdf:type schema:DefinedTerm
    166 sg:journal.1100508 schema:issn 1044-0305
    167 1879-1123
    168 schema:name Journal of The American Society for Mass Spectrometry
    169 rdf:type schema:Periodical
    170 sg:person.01105566503.21 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
    171 schema:familyName Toita
    172 schema:givenName Riki
    173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105566503.21
    174 rdf:type schema:Person
    175 sg:person.01123243142.22 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
    176 schema:familyName Han
    177 schema:givenName Aishan
    178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123243142.22
    179 rdf:type schema:Person
    180 sg:person.01174667253.69 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
    181 schema:familyName Oishi
    182 schema:givenName Jun
    183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174667253.69
    184 rdf:type schema:Person
    185 sg:person.01217553115.49 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
    186 schema:familyName Katayama
    187 schema:givenName Yoshiki
    188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01217553115.49
    189 rdf:type schema:Person
    190 sg:person.01240026312.83 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
    191 schema:familyName Kawamura
    192 schema:givenName Kenji
    193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240026312.83
    194 rdf:type schema:Person
    195 sg:person.01266160666.47 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
    196 schema:familyName Mori
    197 schema:givenName Takeshi
    198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266160666.47
    199 rdf:type schema:Person
    200 sg:person.01324313543.40 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
    201 schema:familyName Shigaki
    202 schema:givenName Shuhei
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324313543.40
    204 rdf:type schema:Person
    205 sg:person.0646211241.31 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
    206 schema:familyName Niidome
    207 schema:givenName Takuro
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646211241.31
    209 rdf:type schema:Person
    210 sg:person.0656131017.90 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
    211 schema:familyName Han
    212 schema:givenName Xiao Ming
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656131017.90
    214 rdf:type schema:Person
    215 sg:person.0760576056.02 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
    216 schema:familyName Kang
    217 schema:givenName Jeong -Hun
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760576056.02
    219 rdf:type schema:Person
    220 sg:pub.10.1007/s10555-005-1578-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015867464
    221 https://doi.org/10.1007/s10555-005-1578-8
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1016/j.jasms.2004.12.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000219791
    224 https://doi.org/10.1016/j.jasms.2004.12.018
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1016/j.jasms.2005.02.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014903416
    227 https://doi.org/10.1016/j.jasms.2005.02.018
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1016/j.jasms.2005.08.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015022515
    230 https://doi.org/10.1016/j.jasms.2005.08.004
    231 rdf:type schema:CreativeWork
    232 sg:pub.10.1365/s10337-006-0810-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028813380
    233 https://doi.org/10.1365/s10337-006-0810-1
    234 rdf:type schema:CreativeWork
    235 https://doi.org/10.1016/0955-0674(90)90006-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1022246267
    236 rdf:type schema:CreativeWork
    237 https://doi.org/10.1016/j.ab.2005.02.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023665894
    238 rdf:type schema:CreativeWork
    239 https://doi.org/10.1016/j.bbapap.2003.11.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008331220
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1016/j.bmcl.2003.12.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007173983
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1016/j.jconrel.2005.10.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035015382
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1016/s0955-0674(97)80058-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014146189
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1021/ac050135t schema:sameAs https://app.dimensions.ai/details/publication/pub.1054996837
    248 rdf:type schema:CreativeWork
    249 https://doi.org/10.1021/ac051519m schema:sameAs https://app.dimensions.ai/details/publication/pub.1054997464
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.1021/bm025532h schema:sameAs https://app.dimensions.ai/details/publication/pub.1055219708
    252 rdf:type schema:CreativeWork
    253 https://doi.org/10.1042/bj20020786 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030698407
    254 rdf:type schema:CreativeWork
    255 https://doi.org/10.1042/bj2830159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007339140
    256 rdf:type schema:CreativeWork
    257 https://doi.org/10.1042/bj2910329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051994416
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.1042/bj2940335 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005284465
    260 rdf:type schema:CreativeWork
    261 https://doi.org/10.1097/00008390-200404000-00002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031557583
    262 rdf:type schema:CreativeWork
    263 https://doi.org/10.1615/critrevoncog.v5.i5.10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068132616
    264 rdf:type schema:CreativeWork
    265 https://doi.org/10.1677/erc.0.0100389 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001145868
    266 rdf:type schema:CreativeWork
    267 https://doi.org/10.2116/analsci.17.277 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045016300
    268 rdf:type schema:CreativeWork
    269 https://www.grid.ac/institutes/grid.177174.3 schema:alternateName Kyushu University
    270 schema:name CREST, Japan Science and Technology Corporation, Kawaguchi, Japan
    271 Center for Future Chemistry, Kyushu University, Fukuoka, Japan
    272 Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, 819-0395, Fukuoka, Japan
    273 rdf:type schema:Organization
    274 https://www.grid.ac/institutes/grid.419082.6 schema:alternateName Japan Science and Technology Agency
    275 schema:name CREST, Japan Science and Technology Corporation, Kawaguchi, Japan
    276 rdf:type schema:Organization
    277 https://www.grid.ac/institutes/grid.7597.c schema:alternateName RIKEN
    278 schema:name Riken, Wako, Japan
    279 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...