Positive electrospray ion trap multistage mass spectrometric fragmentation of synthetic analogs of saccharide part of lipopolysaccharides of Vibrio cholerae O:1 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-06

AUTHORS

Vladimir Kováčik, Slavka Bekešová, Igor Tvaroška, Pavol Kováč

ABSTRACT

Oligosaccharides (mono- to hexamers) that mimic the terminal epitopes of O-antigens of Vibrio cholerae O:1, serotypes Ogawa and Inaba, have been studied by electrospray ion trap (ESI IT) mass spectrometry. Sodium or potassium-cationized adducts are characteristic ions under the conditions of ESI-MS analysis. The tentative pathways of fragmentation have been proven by multistage ion trap MS (MS(n), n = 1-3). The predominant pathway of fragmentation of the oligomers is the neutral loss of monosaccharide residue shortening the length of the oligosaccharide. In this way, conversion of the Ogawa to Inaba fragments takes place under the conditions of measurement. ESI MS/MS provided sufficient information about molecular mass, the number of saccharide residues, and the structure of saccharides, about the C (4)-amide of 3-deoxy-L-glycero-tetronic acid (DGT) of the compounds investigated, and allows to distinguish between Ogawa and Inaba serotypes. More... »

PAGES

749-756

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1016/j.jasms.2006.02.006

DOI

http://dx.doi.org/10.1016/j.jasms.2006.02.006

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019784825

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16600622


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