Peak Height Precision in Hadamard Transform Time-of-Flight Mass Spectra View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2005-07

AUTHORS

Joel R. Kimmel, Oh Kyu Yoon, Ignacio A. Zuleta, Oliver Trapp, Richard N. Zare

ABSTRACT

Hadamard transform (HT) time-of-flight mass spectrometry (TOFMS) is a multiplexing technique that offers high duty cycle for the mass analysis of continuous ion sources. The multiplexing advantage is maximized when spectral noise is independent of signal intensity. For conditions in which shot noise predominates, the variance in each peak is a function of the population of all measured species. We develop expressions for the performance of a HT-TOF mass spectrometer based on Poissonian statistics for the arrival times of ions at the detector. These expressions and complementary probabilistic simulations are used to estimate the magnitude of the baseline noise as a function of mass spectral features and acquisition conditions. Experiment validates the predictions that noise depends on the total number of ions in the acquired spectrum, and the achieved signal-to-noise ratio for a given species depends on its relative population. We find that for HT-TOFMS experiments encoded with an n-order binary off-on sequence that contains N=2n−1 elements, the peak height precision, which is the peak intensity divided by its standard deviation, is greater than that of an equivalent conventional TOF experiment by a factor of √N/2 times the square root of the fractional abundance of the peak of interest. Thus, HT-TOFMS is superior to conventional TOF for all species whose fractional abundance Fi exceeds 2/N, which for a typical N value of 2047 corresponds to Fi > 0.001. HT-TOF mass spectra collected at 2500 per second demonstrates the method’s capability of monitoring transient processes not possible by conventional means. More... »

PAGES

1117-1130

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1016/j.jasms.2005.02.022

DOI

http://dx.doi.org/10.1016/j.jasms.2005.02.022

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https://app.dimensions.ai/details/publication/pub.1049811243


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