Purification of dual-functioning chitinases with hydrolytic and antifreeze activities from Hippophae rhamnoides seedlings View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-03

AUTHORS

Bhavana Sharma, Ravi Gupta, Dinabandhu Sahoo, Renu Deswal

ABSTRACT

Chitinases are glycosyl hydrolases which hydrolyse β-1,4-glycosidic bonds between N-acetylglucosamine residues of chitin. Seabuckthorn (Hippophae rhamnoides), a cold desert plant, is a storehouse of many cold-tolerant proteins including dual-functioning antifreeze proteins (AFPs) possessing both hydrolytic and antifreeze activities. Herein, we report the purification and characterization of antifreeze chitinases from seedlings grown in laboratory conditions. Chitin-affinity chromatography led to homogenous purification of two acidic chitinases HrS CHT1a (33 kDa) and HrS CHT1b (38 kDa) from seedlings. Antifreeze activity of purified AFPs was confirmed by the formation of hexagon-shaped ice crystals using nanolitre osmometer. Similarly, sucrose sandwich splat assay also confirmed their ice recrystallization inhibition activity (1.6-fold decrease in mean ice crystals). The chitinase activity of AFPs was confirmed by chitin hydrolytic assay where higher activity (1.8-fold) was observed in HrS CHT1b (500 U/mg) than HrS CHT1a (222 U/mg). MS identification showed homology of HrS CHT1b with provicilin while HrS CHT1a was identified as uncharacterized protein. In silico analysis showed that purified AFPs differ significantly in biochemical properties which suggests their different physiological roles. Protein association network analysis using string showed interaction of HrS CHT1b with enzymes involved majorly in pathogenic protection (pectinesterase, glycosyl hydrolase protein with chitinase domain). However, HrSCHT1a showed interaction with proteins associated with growth and energy regulation (glycine and purine synthesis, vitamin B metabolism) thereby indicating differential functional roles of both the chitinases. Conserved domain analysis also supported that these AFPs are multifunctional and exhibit differential regulatory roles in enabling the plant growth and defense responses. Further validation of these targets may open gates for commercial utilization of this plant growing abundantly in the Himalayan regions of India for protection of freeze-susceptible crops or biomedical applications. More... »

PAGES

69-81

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s42485-019-00007-9

DOI

http://dx.doi.org/10.1007/s42485-019-00007-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112159341


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Delhi", 
          "id": "https://www.grid.ac/institutes/grid.8195.5", 
          "name": [
            "Molecular Physiology and Proteomics Laboratory, Department of Botany, University of Delhi, 110007, Delhi, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sharma", 
        "givenName": "Bhavana", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pusan National University", 
          "id": "https://www.grid.ac/institutes/grid.262229.f", 
          "name": [
            "Department of Plant Bioscience, College of Natural Resources and Life Sciences, Pusan National University, Miryang, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gupta", 
        "givenName": "Ravi", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Bio-Resources and Sustainable Development", 
          "id": "https://www.grid.ac/institutes/grid.464584.f", 
          "name": [
            "Department of Biotechnology, Institute of Bioresources and Sustainable Development, Imphal, Manipur, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sahoo", 
        "givenName": "Dinabandhu", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Delhi", 
          "id": "https://www.grid.ac/institutes/grid.8195.5", 
          "name": [
            "Molecular Physiology and Proteomics Laboratory, Department of Botany, University of Delhi, 110007, Delhi, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Deswal", 
        "givenName": "Renu", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1104/pp.100.2.593", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002780043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.fbp.2010.04.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003003833"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cryobiol.2009.01.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003859926"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jipa.1999.4843", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005240131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10535-009-0126-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006336092", 
          "https://doi.org/10.1007/s10535-009-0126-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10535-009-0126-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006336092", 
          "https://doi.org/10.1007/s10535-009-0126-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0031-9422(03)00420-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008388845"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0031-9422(03)00420-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008388845"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1997.tb00556.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008741958"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0008654", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012059259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.109.3.879", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013938614"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-9452(99)00069-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015785125"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0063779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020737124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1271/bbb1961.35.1154", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020765446"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0091723", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021386981"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tplants.2004.06.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023767699"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1034/j.1399-3054.1999.105402.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024556141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0734-9750(95)02001-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027353573"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.124.3.1251", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028514161"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.106.081935", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028528390"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.84.19.6750", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029279002"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1014062714786", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030966275", 
          "https://doi.org/10.1023/a:1014062714786"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.plaphy.2011.10.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033586282"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0141-0229(00)00134-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034421903"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0969-2126(01)00229-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037221001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3400385", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037272367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3400385", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037272367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.btre.2017.01.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038227566"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/07352689.2011.616043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040891545"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.3.881", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042852976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2229-6-33", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046371540", 
          "https://doi.org/10.1186/1471-2229-6-33"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cryobiol.2005.06.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046803690"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10535-011-0019-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046896399", 
          "https://doi.org/10.1007/s10535-011-0019-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.126.3.1299", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050604942"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.106.081497", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050871980"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/cryo.2000.2289", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050935893"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/pr200944z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056292817"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.104.3.971", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060840292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1115145", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062452430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/psb.5.2.10336", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072309211"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077900462", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.plantsci.2017.03.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084102330"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-04518-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1090350962", 
          "https://doi.org/10.1038/s41598-017-04518-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.17.00433", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091932832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.17.00433", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091932832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jssc.201800553", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1106287423"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-03", 
    "datePublishedReg": "2019-03-01", 
    "description": "Chitinases are glycosyl hydrolases which hydrolyse \u03b2-1,4-glycosidic bonds between N-acetylglucosamine residues of chitin. Seabuckthorn (Hippophae rhamnoides), a cold desert plant, is a storehouse of many cold-tolerant proteins including dual-functioning antifreeze proteins (AFPs) possessing both hydrolytic and antifreeze activities. Herein, we report the purification and characterization of antifreeze chitinases from seedlings grown in laboratory conditions. Chitin-affinity chromatography led to homogenous purification of two acidic chitinases HrS CHT1a (33 kDa) and HrS CHT1b (38 kDa) from seedlings. Antifreeze activity of purified AFPs was confirmed by the formation of hexagon-shaped ice crystals using nanolitre osmometer. Similarly, sucrose sandwich splat assay also confirmed their ice recrystallization inhibition activity (1.6-fold decrease in mean ice crystals). The chitinase activity of AFPs was confirmed by chitin hydrolytic assay where higher activity (1.8-fold) was observed in HrS CHT1b (500 U/mg) than HrS CHT1a (222 U/mg). MS identification showed homology of HrS CHT1b with provicilin while HrS CHT1a was identified as uncharacterized protein. In silico analysis showed that purified AFPs differ significantly in biochemical properties which suggests their different physiological roles. Protein association network analysis using string showed interaction of HrS CHT1b with enzymes involved majorly in pathogenic protection (pectinesterase, glycosyl hydrolase protein with chitinase domain). However, HrSCHT1a showed interaction with proteins associated with growth and energy regulation (glycine and purine synthesis, vitamin B metabolism) thereby indicating differential functional roles of both the chitinases. Conserved domain analysis also supported that these AFPs are multifunctional and exhibit differential regulatory roles in enabling the plant growth and defense responses. Further validation of these targets may open gates for commercial utilization of this plant growing abundantly in the Himalayan regions of India for protection of freeze-susceptible crops or biomedical applications.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s42485-019-00007-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1285050", 
        "issn": [
          "0975-8151", 
          "2524-4663"
        ], 
        "name": "Journal of Proteins and Proteomics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "Purification of dual-functioning chitinases with hydrolytic and antifreeze activities from Hippophae rhamnoides seedlings", 
    "pagination": "69-81", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "107cf85710945e448a7359da441d0f5b419b3a8be677675e32acab603f450bfb"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s42485-019-00007-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112159341"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s42485-019-00007-9", 
      "https://app.dimensions.ai/details/publication/pub.1112159341"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000366_0000000366/records_112035_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs42485-019-00007-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s42485-019-00007-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s42485-019-00007-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s42485-019-00007-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s42485-019-00007-9'


 

This table displays all metadata directly associated to this object as RDF triples.

221 TRIPLES      21 PREDICATES      71 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s42485-019-00007-9 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N316da6a31b7e4431a551476d9f6909ca
4 schema:citation sg:pub.10.1007/s10535-009-0126-8
5 sg:pub.10.1007/s10535-011-0019-5
6 sg:pub.10.1023/a:1014062714786
7 sg:pub.10.1038/227680a0
8 sg:pub.10.1038/s41598-017-04518-7
9 sg:pub.10.1186/1471-2229-6-33
10 https://app.dimensions.ai/details/publication/pub.1077900462
11 https://doi.org/10.1002/jssc.201800553
12 https://doi.org/10.1006/cryo.2000.2289
13 https://doi.org/10.1006/jipa.1999.4843
14 https://doi.org/10.1016/0003-2697(76)90527-3
15 https://doi.org/10.1016/0734-9750(95)02001-j
16 https://doi.org/10.1016/j.btre.2017.01.001
17 https://doi.org/10.1016/j.cryobiol.2005.06.005
18 https://doi.org/10.1016/j.cryobiol.2009.01.007
19 https://doi.org/10.1016/j.fbp.2010.04.002
20 https://doi.org/10.1016/j.plantsci.2017.03.004
21 https://doi.org/10.1016/j.plaphy.2011.10.005
22 https://doi.org/10.1016/j.tplants.2004.06.007
23 https://doi.org/10.1016/s0031-9422(03)00420-5
24 https://doi.org/10.1016/s0141-0229(00)00134-4
25 https://doi.org/10.1016/s0168-9452(99)00069-2
26 https://doi.org/10.1016/s0969-2126(01)00229-5
27 https://doi.org/10.1021/pr200944z
28 https://doi.org/10.1034/j.1399-3054.1999.105402.x
29 https://doi.org/10.1042/bj3400385
30 https://doi.org/10.1073/pnas.84.19.6750
31 https://doi.org/10.1073/pnas.86.3.881
32 https://doi.org/10.1080/07352689.2011.616043
33 https://doi.org/10.1104/pp.100.2.593
34 https://doi.org/10.1104/pp.104.3.971
35 https://doi.org/10.1104/pp.106.081497
36 https://doi.org/10.1104/pp.106.081935
37 https://doi.org/10.1104/pp.109.3.879
38 https://doi.org/10.1104/pp.124.3.1251
39 https://doi.org/10.1104/pp.126.3.1299
40 https://doi.org/10.1105/tpc.17.00433
41 https://doi.org/10.1111/j.1399-3054.1997.tb00556.x
42 https://doi.org/10.1126/science.1115145
43 https://doi.org/10.1271/bbb1961.35.1154
44 https://doi.org/10.1371/journal.pone.0008654
45 https://doi.org/10.1371/journal.pone.0063779
46 https://doi.org/10.1371/journal.pone.0091723
47 https://doi.org/10.4161/psb.5.2.10336
48 schema:datePublished 2019-03
49 schema:datePublishedReg 2019-03-01
50 schema:description Chitinases are glycosyl hydrolases which hydrolyse β-1,4-glycosidic bonds between N-acetylglucosamine residues of chitin. Seabuckthorn (Hippophae rhamnoides), a cold desert plant, is a storehouse of many cold-tolerant proteins including dual-functioning antifreeze proteins (AFPs) possessing both hydrolytic and antifreeze activities. Herein, we report the purification and characterization of antifreeze chitinases from seedlings grown in laboratory conditions. Chitin-affinity chromatography led to homogenous purification of two acidic chitinases HrS CHT1a (33 kDa) and HrS CHT1b (38 kDa) from seedlings. Antifreeze activity of purified AFPs was confirmed by the formation of hexagon-shaped ice crystals using nanolitre osmometer. Similarly, sucrose sandwich splat assay also confirmed their ice recrystallization inhibition activity (1.6-fold decrease in mean ice crystals). The chitinase activity of AFPs was confirmed by chitin hydrolytic assay where higher activity (1.8-fold) was observed in HrS CHT1b (500 U/mg) than HrS CHT1a (222 U/mg). MS identification showed homology of HrS CHT1b with provicilin while HrS CHT1a was identified as uncharacterized protein. In silico analysis showed that purified AFPs differ significantly in biochemical properties which suggests their different physiological roles. Protein association network analysis using string showed interaction of HrS CHT1b with enzymes involved majorly in pathogenic protection (pectinesterase, glycosyl hydrolase protein with chitinase domain). However, HrSCHT1a showed interaction with proteins associated with growth and energy regulation (glycine and purine synthesis, vitamin B metabolism) thereby indicating differential functional roles of both the chitinases. Conserved domain analysis also supported that these AFPs are multifunctional and exhibit differential regulatory roles in enabling the plant growth and defense responses. Further validation of these targets may open gates for commercial utilization of this plant growing abundantly in the Himalayan regions of India for protection of freeze-susceptible crops or biomedical applications.
51 schema:genre research_article
52 schema:inLanguage en
53 schema:isAccessibleForFree false
54 schema:isPartOf N8b322438460049df90efe38884bda7ad
55 Nd2daa8b9106949a3bdb3aab79fa8c5ce
56 sg:journal.1285050
57 schema:name Purification of dual-functioning chitinases with hydrolytic and antifreeze activities from Hippophae rhamnoides seedlings
58 schema:pagination 69-81
59 schema:productId N15c77bb20ca949af8823d662400ac3e8
60 N45c6cf3682484d83b3dc0a387b30c556
61 Nbc5273d2acba45c3a6cd603e4a0bb397
62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112159341
63 https://doi.org/10.1007/s42485-019-00007-9
64 schema:sdDatePublished 2019-04-11T13:04
65 schema:sdLicense https://scigraph.springernature.com/explorer/license/
66 schema:sdPublisher Ncf22971f8c804a33948fd486b5167ba3
67 schema:url https://link.springer.com/10.1007%2Fs42485-019-00007-9
68 sgo:license sg:explorer/license/
69 sgo:sdDataset articles
70 rdf:type schema:ScholarlyArticle
71 N061a44975e8c40b89772cd9f7e53435b schema:affiliation https://www.grid.ac/institutes/grid.8195.5
72 schema:familyName Sharma
73 schema:givenName Bhavana
74 rdf:type schema:Person
75 N15c77bb20ca949af8823d662400ac3e8 schema:name doi
76 schema:value 10.1007/s42485-019-00007-9
77 rdf:type schema:PropertyValue
78 N316da6a31b7e4431a551476d9f6909ca rdf:first N061a44975e8c40b89772cd9f7e53435b
79 rdf:rest Nc54e5fb4c60e4631aa75d105a90df1e3
80 N45c6cf3682484d83b3dc0a387b30c556 schema:name readcube_id
81 schema:value 107cf85710945e448a7359da441d0f5b419b3a8be677675e32acab603f450bfb
82 rdf:type schema:PropertyValue
83 N6e396e9167074eafa3447909cd839513 rdf:first Nd150fc58e57e416d8a77d2ac5f5a578e
84 rdf:rest rdf:nil
85 N8b322438460049df90efe38884bda7ad schema:issueNumber 1
86 rdf:type schema:PublicationIssue
87 N93b0a60a68134a9b84563a6eca876267 schema:affiliation https://www.grid.ac/institutes/grid.262229.f
88 schema:familyName Gupta
89 schema:givenName Ravi
90 rdf:type schema:Person
91 N9471a5d78f7e4b3886771160e78cfa78 schema:affiliation https://www.grid.ac/institutes/grid.464584.f
92 schema:familyName Sahoo
93 schema:givenName Dinabandhu
94 rdf:type schema:Person
95 Nbc5273d2acba45c3a6cd603e4a0bb397 schema:name dimensions_id
96 schema:value pub.1112159341
97 rdf:type schema:PropertyValue
98 Nbfd41f3bb4ec404cb4f7d10e31a1d6f2 rdf:first N9471a5d78f7e4b3886771160e78cfa78
99 rdf:rest N6e396e9167074eafa3447909cd839513
100 Nc54e5fb4c60e4631aa75d105a90df1e3 rdf:first N93b0a60a68134a9b84563a6eca876267
101 rdf:rest Nbfd41f3bb4ec404cb4f7d10e31a1d6f2
102 Ncf22971f8c804a33948fd486b5167ba3 schema:name Springer Nature - SN SciGraph project
103 rdf:type schema:Organization
104 Nd150fc58e57e416d8a77d2ac5f5a578e schema:affiliation https://www.grid.ac/institutes/grid.8195.5
105 schema:familyName Deswal
106 schema:givenName Renu
107 rdf:type schema:Person
108 Nd2daa8b9106949a3bdb3aab79fa8c5ce schema:volumeNumber 10
109 rdf:type schema:PublicationVolume
110 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
111 schema:name Biological Sciences
112 rdf:type schema:DefinedTerm
113 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
114 schema:name Biochemistry and Cell Biology
115 rdf:type schema:DefinedTerm
116 sg:journal.1285050 schema:issn 0975-8151
117 2524-4663
118 schema:name Journal of Proteins and Proteomics
119 rdf:type schema:Periodical
120 sg:pub.10.1007/s10535-009-0126-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006336092
121 https://doi.org/10.1007/s10535-009-0126-8
122 rdf:type schema:CreativeWork
123 sg:pub.10.1007/s10535-011-0019-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046896399
124 https://doi.org/10.1007/s10535-011-0019-5
125 rdf:type schema:CreativeWork
126 sg:pub.10.1023/a:1014062714786 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030966275
127 https://doi.org/10.1023/a:1014062714786
128 rdf:type schema:CreativeWork
129 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
130 https://doi.org/10.1038/227680a0
131 rdf:type schema:CreativeWork
132 sg:pub.10.1038/s41598-017-04518-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090350962
133 https://doi.org/10.1038/s41598-017-04518-7
134 rdf:type schema:CreativeWork
135 sg:pub.10.1186/1471-2229-6-33 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046371540
136 https://doi.org/10.1186/1471-2229-6-33
137 rdf:type schema:CreativeWork
138 https://app.dimensions.ai/details/publication/pub.1077900462 schema:CreativeWork
139 https://doi.org/10.1002/jssc.201800553 schema:sameAs https://app.dimensions.ai/details/publication/pub.1106287423
140 rdf:type schema:CreativeWork
141 https://doi.org/10.1006/cryo.2000.2289 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050935893
142 rdf:type schema:CreativeWork
143 https://doi.org/10.1006/jipa.1999.4843 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005240131
144 rdf:type schema:CreativeWork
145 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
146 rdf:type schema:CreativeWork
147 https://doi.org/10.1016/0734-9750(95)02001-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1027353573
148 rdf:type schema:CreativeWork
149 https://doi.org/10.1016/j.btre.2017.01.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038227566
150 rdf:type schema:CreativeWork
151 https://doi.org/10.1016/j.cryobiol.2005.06.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046803690
152 rdf:type schema:CreativeWork
153 https://doi.org/10.1016/j.cryobiol.2009.01.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003859926
154 rdf:type schema:CreativeWork
155 https://doi.org/10.1016/j.fbp.2010.04.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003003833
156 rdf:type schema:CreativeWork
157 https://doi.org/10.1016/j.plantsci.2017.03.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084102330
158 rdf:type schema:CreativeWork
159 https://doi.org/10.1016/j.plaphy.2011.10.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033586282
160 rdf:type schema:CreativeWork
161 https://doi.org/10.1016/j.tplants.2004.06.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023767699
162 rdf:type schema:CreativeWork
163 https://doi.org/10.1016/s0031-9422(03)00420-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008388845
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1016/s0141-0229(00)00134-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034421903
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1016/s0168-9452(99)00069-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015785125
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1016/s0969-2126(01)00229-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037221001
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1021/pr200944z schema:sameAs https://app.dimensions.ai/details/publication/pub.1056292817
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1034/j.1399-3054.1999.105402.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024556141
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1042/bj3400385 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037272367
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1073/pnas.84.19.6750 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029279002
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1073/pnas.86.3.881 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042852976
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1080/07352689.2011.616043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040891545
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1104/pp.100.2.593 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002780043
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1104/pp.104.3.971 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060840292
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1104/pp.106.081497 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050871980
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1104/pp.106.081935 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028528390
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1104/pp.109.3.879 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013938614
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1104/pp.124.3.1251 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028514161
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1104/pp.126.3.1299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050604942
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1105/tpc.17.00433 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091932832
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1111/j.1399-3054.1997.tb00556.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1008741958
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1126/science.1115145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062452430
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1271/bbb1961.35.1154 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020765446
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1371/journal.pone.0008654 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012059259
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1371/journal.pone.0063779 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020737124
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1371/journal.pone.0091723 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021386981
210 rdf:type schema:CreativeWork
211 https://doi.org/10.4161/psb.5.2.10336 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072309211
212 rdf:type schema:CreativeWork
213 https://www.grid.ac/institutes/grid.262229.f schema:alternateName Pusan National University
214 schema:name Department of Plant Bioscience, College of Natural Resources and Life Sciences, Pusan National University, Miryang, South Korea
215 rdf:type schema:Organization
216 https://www.grid.ac/institutes/grid.464584.f schema:alternateName Institute of Bio-Resources and Sustainable Development
217 schema:name Department of Biotechnology, Institute of Bioresources and Sustainable Development, Imphal, Manipur, India
218 rdf:type schema:Organization
219 https://www.grid.ac/institutes/grid.8195.5 schema:alternateName University of Delhi
220 schema:name Molecular Physiology and Proteomics Laboratory, Department of Botany, University of Delhi, 110007, Delhi, India
221 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...