Effect of Different Pretreatment Conditions on Saccharum spontaneum for Cellulase Production by B. subtilis K-18 Through Box–Bhenken Design View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-12-21

AUTHORS

Maria Ghazanfar, Muhammad Irfan, Fouzia Tabssum, Hafiz Abbdullah Shakir, Javed Iqbal Qazi

ABSTRACT

In the present investigation, dilute sulphuric acid pretreatment conditions were optimized for Saccharum spontaneum through Box–Bhenken design of response surface methodology for cellulase production. Cellulase production was carried out in 250 ml capacity Erlenmeyer flask using pretreated S. spontaneum as substrate by Bacillus subtilis K-18 incubated at 50 °C for 24 h of fermentation period. Results revealed that sulphuric acid followed by steam favored cellulase production as compared to sulphuric acid treatment. Maximum FPase production (1.389 IU/ml/min) was achieved under pretreatment condition of 1% H2SO4 concentration, 10% substrate concentration with residence time of 4 h at room temperature followed by steam at 121 °C for 15 min and 15 psi. The proposed model was found significant as revealed by F value, P value and coefficient of determination. The cellulase produced could effectively hydrolyze pretreated substrate releasing total sugars of 12.71 mg/ml after 20 h of incubation at 50 °C. These results suggested that the enzyme could effectively be used in industrial process especially in biofuel production from lignocellulosic biomass. More... »

PAGES

313-320

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s40995-017-0463-y

DOI

http://dx.doi.org/10.1007/s40995-017-0463-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1099731781


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