Verification of Electroporation Models Using the Potato Tuber as In Vitro Simulation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-04-28

AUTHORS

José Alvim Berkenbrock, Guilherme Brasil Pintarelli, Afrânio de Castro Antônio Júnior, Daniela Ota Hisayasu Suzuki

ABSTRACT

Electrochemotherapy (ECT) and Irreversible Electroporation are recent non-thermal techniques employed for the treatment of tumors. These therapies are based on the opening of pores in the cell membrane during application of sufficiently high electric fields. Over the past two decades, clinical trials have validated these therapies in humans. Moreover, ECT has been successfully applied in several species in veterinary clinics. As the therapy depends on the electric field distribution, numerical models and in vitro experiments have an important role in better understanding electric field distribution in biological tissues, especially in anatomically and physiologically complex structures. The potato tuber has emerged as an option for testing the effectiveness of the new electrodes. Potatoes present advantages for several reasons: they are compatible with the 3Rs’ concept for animal testing; they are simple to handle; and their electroporated areas become dark after 6–12 h. In this work, we simulated four numerical models from the literature and compared them to in vitro experiments. Our aim was to determine the model that best describes the observable affected area. This is an important point because the models were developed to properly describe tissue variation in electrical conductivity during electroporation, not to determine how electroporation is manifested macroscopically in potato tissue. The area measured with the Miklavčič–Ivorra–Suárez model was in closest agreement to the experiments; however, none of the models exactly represent our in vitro results. More... »

PAGES

224-229

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s40846-018-0408-8

DOI

http://dx.doi.org/10.1007/s40846-018-0408-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1103675789


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/08", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Information and Computing Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/09", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0801", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Artificial Intelligence and Image Processing", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0903", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biomedical Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0906", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Electrical and Electronic Engineering", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Electrical and Electronics Engineering, Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), 88040-370, Florian\u00f3polis, Santa Catarina, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.411237.2", 
          "name": [
            "Department of Electrical and Computer Engineering, University of Saskatchewan (U of S), S7N 5A9, Saskatoon, Saskatchewan, Canada", 
            "Department of Electrical and Electronics Engineering, Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), 88040-370, Florian\u00f3polis, Santa Catarina, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Berkenbrock", 
        "givenName": "Jos\u00e9 Alvim", 
        "id": "sg:person.07510260715.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07510260715.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Electrical and Electronics Engineering, Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), 88040-370, Florian\u00f3polis, Santa Catarina, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.411237.2", 
          "name": [
            "Department of Electrical and Electronics Engineering, Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), 88040-370, Florian\u00f3polis, Santa Catarina, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brasil Pintarelli", 
        "givenName": "Guilherme", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Electrical and Electronics Engineering, Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), 88040-370, Florian\u00f3polis, Santa Catarina, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.411237.2", 
          "name": [
            "Department of Electrical and Electronics Engineering, Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), 88040-370, Florian\u00f3polis, Santa Catarina, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Castro Ant\u00f4nio J\u00fanior", 
        "givenName": "Afr\u00e2nio", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Electrical and Electronics Engineering, Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), 88040-370, Florian\u00f3polis, Santa Catarina, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.411237.2", 
          "name": [
            "Department of Electrical and Electronics Engineering, Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), 88040-370, Florian\u00f3polis, Santa Catarina, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Suzuki", 
        "givenName": "Daniela Ota Hisayasu", 
        "id": "sg:person.0575513366.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0575513366.69"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1385/1-59259-409-3:55", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051045198", 
          "https://doi.org/10.1385/1-59259-409-3:55"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-981-287-817-5_90", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036231894", 
          "https://doi.org/10.1007/978-981-287-817-5_90"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/268438a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009232519", 
          "https://doi.org/10.1038/268438a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00232-010-9281-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022510733", 
          "https://doi.org/10.1007/s00232-010-9281-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10439-005-8981-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025136074", 
          "https://doi.org/10.1007/s10439-005-8981-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1479-5876-9-152", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039203892", 
          "https://doi.org/10.1186/1479-5876-9-152"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-04-28", 
    "datePublishedReg": "2018-04-28", 
    "description": "Electrochemotherapy (ECT) and Irreversible Electroporation are recent non-thermal techniques employed for the treatment of tumors. These therapies are based on the opening of pores in the cell membrane during application of sufficiently high electric fields. Over the past two decades, clinical trials have validated these therapies in humans. Moreover, ECT has been successfully applied in several species in veterinary clinics. As the therapy depends on the electric field distribution, numerical models and in vitro experiments have an important role in better understanding electric field distribution in biological tissues, especially in anatomically and physiologically complex structures. The potato tuber has emerged as an option for testing the effectiveness of the new electrodes. Potatoes present advantages for several reasons: they are compatible with the 3Rs\u2019 concept for animal testing; they are simple to handle; and their electroporated areas become dark after 6\u201312\u00a0h. In this work, we simulated four numerical models from the literature and compared them to in vitro experiments. Our aim was to determine the model that best describes the observable affected area. This is an important point because the models were developed to properly describe tissue variation in electrical conductivity during electroporation, not to determine how electroporation is manifested macroscopically in potato tissue. The area measured with the Miklav\u010di\u010d\u2013Ivorra\u2013Su\u00e1rez model was in closest agreement to the experiments; however, none of the models exactly represent our in vitro results.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s40846-018-0408-8", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1294980", 
        "issn": [
          "1609-0985", 
          "2199-4757"
        ], 
        "name": "Journal of Medical and Biological Engineering", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "39"
      }
    ], 
    "keywords": [
      "electric field distribution", 
      "numerical model", 
      "field distribution", 
      "high electric field", 
      "electric field", 
      "electrical conductivity", 
      "non-thermal techniques", 
      "electroporation model", 
      "biological tissues", 
      "new electrode", 
      "close agreement", 
      "present advantages", 
      "complex structure", 
      "opening of pores", 
      "conductivity", 
      "potato tissue", 
      "vitro simulation", 
      "pores", 
      "simulations", 
      "experiments", 
      "electrode", 
      "model", 
      "distribution", 
      "verification", 
      "applications", 
      "irreversible electroporation", 
      "area", 
      "field", 
      "advantages", 
      "agreement", 
      "structure", 
      "technique", 
      "affected area", 
      "work", 
      "important point", 
      "testing", 
      "effectiveness", 
      "variation", 
      "results", 
      "point", 
      "tissue variations", 
      "opening", 
      "membrane", 
      "concept", 
      "important role", 
      "electroporation", 
      "electrochemotherapy", 
      "animal testing", 
      "reasons", 
      "options", 
      "decades", 
      "literature", 
      "aim", 
      "tissue", 
      "treatment of tumors", 
      "treatment", 
      "cell membrane", 
      "potato tubers", 
      "role", 
      "tubers", 
      "species", 
      "humans", 
      "trials", 
      "tumors", 
      "veterinary clinics", 
      "therapy", 
      "clinic", 
      "clinical trials", 
      "recent non-thermal techniques", 
      "Potatoes present advantages", 
      "observable affected area", 
      "Miklav\u010di\u010d\u2013Ivorra", 
      "Su\u00e1rez model"
    ], 
    "name": "Verification of Electroporation Models Using the Potato Tuber as In Vitro Simulation", 
    "pagination": "224-229", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1103675789"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s40846-018-0408-8"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s40846-018-0408-8", 
      "https://app.dimensions.ai/details/publication/pub.1103675789"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:42", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_790.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s40846-018-0408-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s40846-018-0408-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s40846-018-0408-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s40846-018-0408-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s40846-018-0408-8'


 

This table displays all metadata directly associated to this object as RDF triples.

187 TRIPLES      22 PREDICATES      107 URIs      90 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s40846-018-0408-8 schema:about anzsrc-for:08
2 anzsrc-for:0801
3 anzsrc-for:09
4 anzsrc-for:0903
5 anzsrc-for:0906
6 schema:author Nac3b3345002643f9a00e0fd169ffde67
7 schema:citation sg:pub.10.1007/978-981-287-817-5_90
8 sg:pub.10.1007/s00232-010-9281-2
9 sg:pub.10.1007/s10439-005-8981-8
10 sg:pub.10.1038/268438a0
11 sg:pub.10.1186/1479-5876-9-152
12 sg:pub.10.1385/1-59259-409-3:55
13 schema:datePublished 2018-04-28
14 schema:datePublishedReg 2018-04-28
15 schema:description Electrochemotherapy (ECT) and Irreversible Electroporation are recent non-thermal techniques employed for the treatment of tumors. These therapies are based on the opening of pores in the cell membrane during application of sufficiently high electric fields. Over the past two decades, clinical trials have validated these therapies in humans. Moreover, ECT has been successfully applied in several species in veterinary clinics. As the therapy depends on the electric field distribution, numerical models and in vitro experiments have an important role in better understanding electric field distribution in biological tissues, especially in anatomically and physiologically complex structures. The potato tuber has emerged as an option for testing the effectiveness of the new electrodes. Potatoes present advantages for several reasons: they are compatible with the 3Rs’ concept for animal testing; they are simple to handle; and their electroporated areas become dark after 6–12 h. In this work, we simulated four numerical models from the literature and compared them to in vitro experiments. Our aim was to determine the model that best describes the observable affected area. This is an important point because the models were developed to properly describe tissue variation in electrical conductivity during electroporation, not to determine how electroporation is manifested macroscopically in potato tissue. The area measured with the Miklavčič–Ivorra–Suárez model was in closest agreement to the experiments; however, none of the models exactly represent our in vitro results.
16 schema:genre article
17 schema:inLanguage en
18 schema:isAccessibleForFree false
19 schema:isPartOf N068ddad73b624b539045b5b0df4e32b4
20 Nce0daf4998644ad4ba28bb4cb5385e22
21 sg:journal.1294980
22 schema:keywords Miklavčič–Ivorra
23 Potatoes present advantages
24 Suárez model
25 advantages
26 affected area
27 agreement
28 aim
29 animal testing
30 applications
31 area
32 biological tissues
33 cell membrane
34 clinic
35 clinical trials
36 close agreement
37 complex structure
38 concept
39 conductivity
40 decades
41 distribution
42 effectiveness
43 electric field
44 electric field distribution
45 electrical conductivity
46 electrochemotherapy
47 electrode
48 electroporation
49 electroporation model
50 experiments
51 field
52 field distribution
53 high electric field
54 humans
55 important point
56 important role
57 irreversible electroporation
58 literature
59 membrane
60 model
61 new electrode
62 non-thermal techniques
63 numerical model
64 observable affected area
65 opening
66 opening of pores
67 options
68 point
69 pores
70 potato tissue
71 potato tubers
72 present advantages
73 reasons
74 recent non-thermal techniques
75 results
76 role
77 simulations
78 species
79 structure
80 technique
81 testing
82 therapy
83 tissue
84 tissue variations
85 treatment
86 treatment of tumors
87 trials
88 tubers
89 tumors
90 variation
91 verification
92 veterinary clinics
93 vitro simulation
94 work
95 schema:name Verification of Electroporation Models Using the Potato Tuber as In Vitro Simulation
96 schema:pagination 224-229
97 schema:productId N00e9cc72251a4f95bd90047612dfcbc2
98 N8a8508666bce4c548cd25fa5efc76c81
99 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103675789
100 https://doi.org/10.1007/s40846-018-0408-8
101 schema:sdDatePublished 2021-12-01T19:42
102 schema:sdLicense https://scigraph.springernature.com/explorer/license/
103 schema:sdPublisher Nd2115d4126da48eab29f60584fffe1e5
104 schema:url https://doi.org/10.1007/s40846-018-0408-8
105 sgo:license sg:explorer/license/
106 sgo:sdDataset articles
107 rdf:type schema:ScholarlyArticle
108 N001c44b675064569bd59bd3424958f93 schema:affiliation grid-institutes:grid.411237.2
109 schema:familyName de Castro Antônio Júnior
110 schema:givenName Afrânio
111 rdf:type schema:Person
112 N00e9cc72251a4f95bd90047612dfcbc2 schema:name doi
113 schema:value 10.1007/s40846-018-0408-8
114 rdf:type schema:PropertyValue
115 N068ddad73b624b539045b5b0df4e32b4 schema:volumeNumber 39
116 rdf:type schema:PublicationVolume
117 N38cdc3c775b24f27a6b5a7ac7940b4a8 rdf:first N001c44b675064569bd59bd3424958f93
118 rdf:rest N3e1005486cee4c53ad54f48a9b433ab3
119 N3e1005486cee4c53ad54f48a9b433ab3 rdf:first sg:person.0575513366.69
120 rdf:rest rdf:nil
121 N648156bc7b72408eadacd1d501cfd00a schema:affiliation grid-institutes:grid.411237.2
122 schema:familyName Brasil Pintarelli
123 schema:givenName Guilherme
124 rdf:type schema:Person
125 N8a8508666bce4c548cd25fa5efc76c81 schema:name dimensions_id
126 schema:value pub.1103675789
127 rdf:type schema:PropertyValue
128 Nac3b3345002643f9a00e0fd169ffde67 rdf:first sg:person.07510260715.15
129 rdf:rest Nf75ab7fe2e574b0791cdd66205b3f8cb
130 Nce0daf4998644ad4ba28bb4cb5385e22 schema:issueNumber 2
131 rdf:type schema:PublicationIssue
132 Nd2115d4126da48eab29f60584fffe1e5 schema:name Springer Nature - SN SciGraph project
133 rdf:type schema:Organization
134 Nf75ab7fe2e574b0791cdd66205b3f8cb rdf:first N648156bc7b72408eadacd1d501cfd00a
135 rdf:rest N38cdc3c775b24f27a6b5a7ac7940b4a8
136 anzsrc-for:08 schema:inDefinedTermSet anzsrc-for:
137 schema:name Information and Computing Sciences
138 rdf:type schema:DefinedTerm
139 anzsrc-for:0801 schema:inDefinedTermSet anzsrc-for:
140 schema:name Artificial Intelligence and Image Processing
141 rdf:type schema:DefinedTerm
142 anzsrc-for:09 schema:inDefinedTermSet anzsrc-for:
143 schema:name Engineering
144 rdf:type schema:DefinedTerm
145 anzsrc-for:0903 schema:inDefinedTermSet anzsrc-for:
146 schema:name Biomedical Engineering
147 rdf:type schema:DefinedTerm
148 anzsrc-for:0906 schema:inDefinedTermSet anzsrc-for:
149 schema:name Electrical and Electronic Engineering
150 rdf:type schema:DefinedTerm
151 sg:journal.1294980 schema:issn 1609-0985
152 2199-4757
153 schema:name Journal of Medical and Biological Engineering
154 schema:publisher Springer Nature
155 rdf:type schema:Periodical
156 sg:person.0575513366.69 schema:affiliation grid-institutes:grid.411237.2
157 schema:familyName Suzuki
158 schema:givenName Daniela Ota Hisayasu
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0575513366.69
160 rdf:type schema:Person
161 sg:person.07510260715.15 schema:affiliation grid-institutes:grid.411237.2
162 schema:familyName Berkenbrock
163 schema:givenName José Alvim
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07510260715.15
165 rdf:type schema:Person
166 sg:pub.10.1007/978-981-287-817-5_90 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036231894
167 https://doi.org/10.1007/978-981-287-817-5_90
168 rdf:type schema:CreativeWork
169 sg:pub.10.1007/s00232-010-9281-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022510733
170 https://doi.org/10.1007/s00232-010-9281-2
171 rdf:type schema:CreativeWork
172 sg:pub.10.1007/s10439-005-8981-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025136074
173 https://doi.org/10.1007/s10439-005-8981-8
174 rdf:type schema:CreativeWork
175 sg:pub.10.1038/268438a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009232519
176 https://doi.org/10.1038/268438a0
177 rdf:type schema:CreativeWork
178 sg:pub.10.1186/1479-5876-9-152 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039203892
179 https://doi.org/10.1186/1479-5876-9-152
180 rdf:type schema:CreativeWork
181 sg:pub.10.1385/1-59259-409-3:55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051045198
182 https://doi.org/10.1385/1-59259-409-3:55
183 rdf:type schema:CreativeWork
184 grid-institutes:grid.411237.2 schema:alternateName Department of Electrical and Electronics Engineering, Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), 88040-370, Florianópolis, Santa Catarina, Brazil
185 schema:name Department of Electrical and Computer Engineering, University of Saskatchewan (U of S), S7N 5A9, Saskatoon, Saskatchewan, Canada
186 Department of Electrical and Electronics Engineering, Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), 88040-370, Florianópolis, Santa Catarina, Brazil
187 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...