A silk fibroin/decellularized extract of Wharton’s jelly hydrogel intended for cartilage tissue engineering View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-03

AUTHORS

Arefeh Basiri, Mehdi Farokhi, Mahmoud Azami, Somayeh Ebrahimi-Barough, Abdolreza Mohamadnia, Morteza Rashtbar, Elham Hasanzadeh, Narges Mahmoodi, Mohamadreza Baghaban Eslaminejad, Jafar Ai

ABSTRACT

A hybrid hydrogel was obtained from decellularized extract from Wharton's jelly (DEWJ) and silk fibroin (SF) and characterized for cartilage tissue engineering. Wharton's jelly was used due to its similarity with articular cartilage in extracellular matrix composition. Also, silk fibroin has good mechanical properties which make this construct appropriate for cartilage repair. Decellularization of Wharton's jelly was verified by DAPI staining, DNA quantification, and PCR analysis. Then, the biochemical composition of DEWJ was determined by ELISA kits for total proteins, collagens, sulfated glycosaminoglycans (sGAG), and transforming growth factor β1 (TGF-β1). After fabricating pure SF and SF/DEWJ hybrid hydrogels, their physical and mechanical properties were characterized by FESEM, Fourier-transform infrared spectroscopy (FTIR) and rheological assays (amplitude and frequency sweeps). Furthermore, cell viability and proliferation were assessed by MTT assay. The results have shown that DEWJ in hybrid hydrogels enhances mechanical properties of the construct relative to pure SF hydrogels. Also, this extract at its 40% concentration in culture media and 20% or 40% concentrations in SF/DEWJ hybrid hydrogels significantly increases population of the cells compared to control and pure SF hydrogel after 7 days. In conclusion, this study proposes the potential of SF/DEWJ hybrid hydrogels for cartilage tissue engineering applications. More... »

PAGES

31-42

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s40204-019-0108-7

DOI

http://dx.doi.org/10.1007/s40204-019-0108-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111824491

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30706299


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