Establishment of adult mouse testis-derived multipotent germ line stem cells and comparison of lineage-specific differentiation potential View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-04

AUTHORS

Bang-Jin Kim, Yong-An Lee, Yong-Hee Kim, Ki-Jung Kim, Mi-Seon Jung, Seung-Jung Ha, Hyun-Gu Kang, Byung-Gak Kim, Jeong Tae Do, Hoe Saeng Yang, Buom-Yong Ryu

ABSTRACT

Primordial germ cells (PGCs) as well as fetal germ line stem cells (GSCs) are pluripotent cells. Their differentiation potential is similar to that of embryonic stem cells (ESCs), suggesting that germ line lineage may retain the potential to form pluripotent cells. Here we report successful establishment of multipotent germ line stem cells (mGSCs) from cells obtained from adult mouse testis. We obtained testes from Pou5f1 (Oct4)-GFP transgenic mice and demonstrate that the germ cells present in testes can be converted to pluripotent stem cell-like cells. The newly established mGSCs had similar morphology and growth characteristics as that of ESCs and expressed markers of pluripotency. To assess the differentiation potential of mGSCs in vivo and in vitro, we performed a teratoma formation assay and in vitro differentiation via embryoid body (EB) formation. Multipotent GSC-derived teratomas contained derivatives of all the three embryonic germ layers and differentiated into multiple lineages in vitro, including cardiomyocytes and neural cells. Using real-time qPCR analysis, we compared the gene expression pattern in the newly established mGSCs with those of pluripotent stem cells, germ cells, and testicular cells. The differentiation potential of the newly established mGSCs was comparable to that of ESCs and induced pluripotent stem cells (iPSCs). Multipotent GSCs derived from adult testes can be used for personalized cell-based therapies without the ethical and immunological problems. More... »

PAGES

121-130

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13770-014-0063-2

DOI

http://dx.doi.org/10.1007/s13770-014-0063-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027166082


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Bang-Jin", 
        "id": "sg:person.0706046135.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706046135.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National University of Singapore", 
          "id": "https://www.grid.ac/institutes/grid.4280.e", 
          "name": [
            "Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea", 
            "Department of Chemistry, National University of Singapore, Singapore, Republic of Singapore"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Yong-An", 
        "id": "sg:person.01326265344.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326265344.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Yong-Hee", 
        "id": "sg:person.0602661244.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602661244.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Ki-Jung", 
        "id": "sg:person.0730322707.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730322707.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jung", 
        "givenName": "Mi-Seon", 
        "id": "sg:person.012235725505.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012235725505.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ha", 
        "givenName": "Seung-Jung", 
        "id": "sg:person.01270352304.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270352304.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kang", 
        "givenName": "Hyun-Gu", 
        "id": "sg:person.016313412544.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016313412544.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Michigan State University", 
          "id": "https://www.grid.ac/institutes/grid.17088.36", 
          "name": [
            "Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea", 
            "Obstetrics, Gynecology & Reproductive Biology, Michigan State University, East Lansing, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Byung-Gak", 
        "id": "sg:person.01315223635.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315223635.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Konkuk University", 
          "id": "https://www.grid.ac/institutes/grid.258676.8", 
          "name": [
            "Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Do", 
        "givenName": "Jeong Tae", 
        "id": "sg:person.07651674305.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07651674305.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Dongguk University", 
          "id": "https://www.grid.ac/institutes/grid.255168.d", 
          "name": [
            "Department of Obstetrics and Gynecology, Dongguk University, Medical college Geongju, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Hoe Saeng", 
        "id": "sg:person.013127260705.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013127260705.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ryu", 
        "givenName": "Buom-Yong", 
        "id": "sg:person.01171073502.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171073502.48"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/343657a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001498814", 
          "https://doi.org/10.1038/343657a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.11.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001802823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod.103.017012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002372289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.10.5504", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002967475"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.9.17.2105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003744681"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0925-4773(98)00002-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004003754"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(02)00432-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004343393"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(91)90573-h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004492987"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(03)00392-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004929245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005967947", 
          "https://doi.org/10.1038/nature07404"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mod.2004.08.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006327361"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0506970102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008600171"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.97.15.8346", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009438925"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/stem.5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014011519"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04697", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014036272", 
          "https://doi.org/10.1038/nature04697"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04697", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014036272", 
          "https://doi.org/10.1038/nature04697"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04697", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014036272", 
          "https://doi.org/10.1038/nature04697"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014573758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1567-133x(03)00005-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014712446"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/74199", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018147371", 
          "https://doi.org/10.1038/74199"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/74199", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018147371", 
          "https://doi.org/10.1038/74199"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/344435a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018230461", 
          "https://doi.org/10.1038/344435a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/exnr.1999.7319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018311210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0905657107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021649064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2004.11.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024217468"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1438", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028911022", 
          "https://doi.org/10.1038/nrm1438"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1438", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028911022", 
          "https://doi.org/10.1038/nrm1438"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature06129", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030896342", 
          "https://doi.org/10.1038/nature06129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ydbio.2004.07.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032636156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(03)00393-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033478972"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod.107.066068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037408546"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2007-02-074120", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037729391"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2148-7-164", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038570894", 
          "https://doi.org/10.1186/1471-2148-7-164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2009.05.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039248669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/dvdy.20034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041414983"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(92)90550-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041949611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(90)90597-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043021105"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ar.1091690306", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046232846"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0407063101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046300934"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.97.26.14720", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046407009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1151526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048905674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1082940", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050452884"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/153623003321512193", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059214910"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.15.1.141", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062713624"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1530/rep-07-0479", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067716975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076896194", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/physrev.1972.52.1.198", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1080482667"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082886299", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-04", 
    "datePublishedReg": "2014-04-01", 
    "description": "Primordial germ cells (PGCs) as well as fetal germ line stem cells (GSCs) are pluripotent cells. Their differentiation potential is similar to that of embryonic stem cells (ESCs), suggesting that germ line lineage may retain the potential to form pluripotent cells. Here we report successful establishment of multipotent germ line stem cells (mGSCs) from cells obtained from adult mouse testis. We obtained testes from Pou5f1 (Oct4)-GFP transgenic mice and demonstrate that the germ cells present in testes can be converted to pluripotent stem cell-like cells. The newly established mGSCs had similar morphology and growth characteristics as that of ESCs and expressed markers of pluripotency. To assess the differentiation potential of mGSCs in vivo and in vitro, we performed a teratoma formation assay and in vitro differentiation via embryoid body (EB) formation. Multipotent GSC-derived teratomas contained derivatives of all the three embryonic germ layers and differentiated into multiple lineages in vitro, including cardiomyocytes and neural cells. Using real-time qPCR analysis, we compared the gene expression pattern in the newly established mGSCs with those of pluripotent stem cells, germ cells, and testicular cells. The differentiation potential of the newly established mGSCs was comparable to that of ESCs and induced pluripotent stem cells (iPSCs). Multipotent GSCs derived from adult testes can be used for personalized cell-based therapies without the ethical and immunological problems.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s13770-014-0063-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1040173", 
        "issn": [
          "1738-2696", 
          "2212-5469"
        ], 
        "name": "Tissue Engineering and Regenerative Medicine", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "11"
      }
    ], 
    "name": "Establishment of adult mouse testis-derived multipotent germ line stem cells and comparison of lineage-specific differentiation potential", 
    "pagination": "121-130", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "816899ab67a6fc8aa6ba90c3ff5b77c61532063fdc3de2489458f487fff3a857"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s13770-014-0063-2"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1027166082"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s13770-014-0063-2", 
      "https://app.dimensions.ai/details/publication/pub.1027166082"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T02:08", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000522.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs13770-014-0063-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s13770-014-0063-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s13770-014-0063-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s13770-014-0063-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s13770-014-0063-2'


 

This table displays all metadata directly associated to this object as RDF triples.

283 TRIPLES      21 PREDICATES      71 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s13770-014-0063-2 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author N775a1fca9a7042d58c1a20d9f94a0b1e
4 schema:citation sg:pub.10.1038/343657a0
5 sg:pub.10.1038/344435a0
6 sg:pub.10.1038/74199
7 sg:pub.10.1038/nature04697
8 sg:pub.10.1038/nature06129
9 sg:pub.10.1038/nature07404
10 sg:pub.10.1038/nrm1438
11 sg:pub.10.1186/1471-2148-7-164
12 https://app.dimensions.ai/details/publication/pub.1076896194
13 https://app.dimensions.ai/details/publication/pub.1082886299
14 https://doi.org/10.1002/ar.1091690306
15 https://doi.org/10.1002/dvdy.20034
16 https://doi.org/10.1002/stem.5
17 https://doi.org/10.1006/exnr.1999.7319
18 https://doi.org/10.1016/0092-8674(90)90597-8
19 https://doi.org/10.1016/0092-8674(91)90573-h
20 https://doi.org/10.1016/0092-8674(92)90550-v
21 https://doi.org/10.1016/j.cell.2004.11.011
22 https://doi.org/10.1016/j.cell.2006.07.024
23 https://doi.org/10.1016/j.cell.2006.11.016
24 https://doi.org/10.1016/j.mod.2004.08.008
25 https://doi.org/10.1016/j.stem.2009.05.025
26 https://doi.org/10.1016/j.ydbio.2004.07.004
27 https://doi.org/10.1016/s0092-8674(03)00392-1
28 https://doi.org/10.1016/s0092-8674(03)00393-3
29 https://doi.org/10.1016/s0925-4773(98)00002-1
30 https://doi.org/10.1016/s0960-9822(02)00432-3
31 https://doi.org/10.1016/s1567-133x(03)00005-x
32 https://doi.org/10.1073/pnas.0407063101
33 https://doi.org/10.1073/pnas.0506970102
34 https://doi.org/10.1073/pnas.0905657107
35 https://doi.org/10.1073/pnas.96.10.5504
36 https://doi.org/10.1073/pnas.97.15.8346
37 https://doi.org/10.1073/pnas.97.26.14720
38 https://doi.org/10.1089/153623003321512193
39 https://doi.org/10.1095/biolreprod.103.017012
40 https://doi.org/10.1095/biolreprod.107.066068
41 https://doi.org/10.1101/gad.9.17.2105
42 https://doi.org/10.1126/science.1082940
43 https://doi.org/10.1126/science.1151526
44 https://doi.org/10.1128/mcb.15.1.141
45 https://doi.org/10.1152/physrev.1972.52.1.198
46 https://doi.org/10.1182/blood-2007-02-074120
47 https://doi.org/10.1530/rep-07-0479
48 schema:datePublished 2014-04
49 schema:datePublishedReg 2014-04-01
50 schema:description Primordial germ cells (PGCs) as well as fetal germ line stem cells (GSCs) are pluripotent cells. Their differentiation potential is similar to that of embryonic stem cells (ESCs), suggesting that germ line lineage may retain the potential to form pluripotent cells. Here we report successful establishment of multipotent germ line stem cells (mGSCs) from cells obtained from adult mouse testis. We obtained testes from Pou5f1 (Oct4)-GFP transgenic mice and demonstrate that the germ cells present in testes can be converted to pluripotent stem cell-like cells. The newly established mGSCs had similar morphology and growth characteristics as that of ESCs and expressed markers of pluripotency. To assess the differentiation potential of mGSCs in vivo and in vitro, we performed a teratoma formation assay and in vitro differentiation via embryoid body (EB) formation. Multipotent GSC-derived teratomas contained derivatives of all the three embryonic germ layers and differentiated into multiple lineages in vitro, including cardiomyocytes and neural cells. Using real-time qPCR analysis, we compared the gene expression pattern in the newly established mGSCs with those of pluripotent stem cells, germ cells, and testicular cells. The differentiation potential of the newly established mGSCs was comparable to that of ESCs and induced pluripotent stem cells (iPSCs). Multipotent GSCs derived from adult testes can be used for personalized cell-based therapies without the ethical and immunological problems.
51 schema:genre research_article
52 schema:inLanguage en
53 schema:isAccessibleForFree false
54 schema:isPartOf N1ad07366bd594756a562a355a5e31ab3
55 Nbc994b188df444cb9a5ea9c57a611ab0
56 sg:journal.1040173
57 schema:name Establishment of adult mouse testis-derived multipotent germ line stem cells and comparison of lineage-specific differentiation potential
58 schema:pagination 121-130
59 schema:productId N186914d3fcfd4bc5b7ad9c67e4156e73
60 Nb75460f61b1f4eb396ebda411c04e256
61 Nd0261e41299545fd8df948f523835a24
62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027166082
63 https://doi.org/10.1007/s13770-014-0063-2
64 schema:sdDatePublished 2019-04-11T02:08
65 schema:sdLicense https://scigraph.springernature.com/explorer/license/
66 schema:sdPublisher N575fe38387d6480a91126c3b08c59860
67 schema:url http://link.springer.com/10.1007%2Fs13770-014-0063-2
68 sgo:license sg:explorer/license/
69 sgo:sdDataset articles
70 rdf:type schema:ScholarlyArticle
71 N16b496f09ee84eae956444e23d19fb42 rdf:first sg:person.0602661244.23
72 rdf:rest Nfa6c7e6065584daa8fa3190598c1b3fb
73 N186914d3fcfd4bc5b7ad9c67e4156e73 schema:name readcube_id
74 schema:value 816899ab67a6fc8aa6ba90c3ff5b77c61532063fdc3de2489458f487fff3a857
75 rdf:type schema:PropertyValue
76 N1ad07366bd594756a562a355a5e31ab3 schema:issueNumber 2
77 rdf:type schema:PublicationIssue
78 N24ece8c495ab4dfe93296f3d5fdbbd41 rdf:first sg:person.01171073502.48
79 rdf:rest rdf:nil
80 N2ccf6e38acc44ca3a0cd0c5702a11be2 rdf:first sg:person.012235725505.25
81 rdf:rest N4e5a3bbf1fcb4cd68aaa48e896d53d19
82 N4c164f52a2b4437bbf3e7c4c63b4d8fc rdf:first sg:person.013127260705.20
83 rdf:rest N24ece8c495ab4dfe93296f3d5fdbbd41
84 N4e5a3bbf1fcb4cd68aaa48e896d53d19 rdf:first sg:person.01270352304.00
85 rdf:rest N4ee2bd62c36d4acd8d7453321f95aaee
86 N4ee2bd62c36d4acd8d7453321f95aaee rdf:first sg:person.016313412544.37
87 rdf:rest Na510e8671cca4d6aa2305e32e4808d30
88 N575fe38387d6480a91126c3b08c59860 schema:name Springer Nature - SN SciGraph project
89 rdf:type schema:Organization
90 N775a1fca9a7042d58c1a20d9f94a0b1e rdf:first sg:person.0706046135.57
91 rdf:rest Ndad242cf5a514358a36dd569f01d452d
92 N8a1daecf59be46d4b07a0db6ca149714 rdf:first sg:person.07651674305.71
93 rdf:rest N4c164f52a2b4437bbf3e7c4c63b4d8fc
94 Na510e8671cca4d6aa2305e32e4808d30 rdf:first sg:person.01315223635.05
95 rdf:rest N8a1daecf59be46d4b07a0db6ca149714
96 Nb75460f61b1f4eb396ebda411c04e256 schema:name dimensions_id
97 schema:value pub.1027166082
98 rdf:type schema:PropertyValue
99 Nbc994b188df444cb9a5ea9c57a611ab0 schema:volumeNumber 11
100 rdf:type schema:PublicationVolume
101 Nd0261e41299545fd8df948f523835a24 schema:name doi
102 schema:value 10.1007/s13770-014-0063-2
103 rdf:type schema:PropertyValue
104 Ndad242cf5a514358a36dd569f01d452d rdf:first sg:person.01326265344.33
105 rdf:rest N16b496f09ee84eae956444e23d19fb42
106 Nfa6c7e6065584daa8fa3190598c1b3fb rdf:first sg:person.0730322707.27
107 rdf:rest N2ccf6e38acc44ca3a0cd0c5702a11be2
108 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
109 schema:name Biological Sciences
110 rdf:type schema:DefinedTerm
111 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
112 schema:name Genetics
113 rdf:type schema:DefinedTerm
114 sg:journal.1040173 schema:issn 1738-2696
115 2212-5469
116 schema:name Tissue Engineering and Regenerative Medicine
117 rdf:type schema:Periodical
118 sg:person.01171073502.48 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
119 schema:familyName Ryu
120 schema:givenName Buom-Yong
121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171073502.48
122 rdf:type schema:Person
123 sg:person.012235725505.25 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
124 schema:familyName Jung
125 schema:givenName Mi-Seon
126 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012235725505.25
127 rdf:type schema:Person
128 sg:person.01270352304.00 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
129 schema:familyName Ha
130 schema:givenName Seung-Jung
131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270352304.00
132 rdf:type schema:Person
133 sg:person.013127260705.20 schema:affiliation https://www.grid.ac/institutes/grid.255168.d
134 schema:familyName Yang
135 schema:givenName Hoe Saeng
136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013127260705.20
137 rdf:type schema:Person
138 sg:person.01315223635.05 schema:affiliation https://www.grid.ac/institutes/grid.17088.36
139 schema:familyName Kim
140 schema:givenName Byung-Gak
141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315223635.05
142 rdf:type schema:Person
143 sg:person.01326265344.33 schema:affiliation https://www.grid.ac/institutes/grid.4280.e
144 schema:familyName Lee
145 schema:givenName Yong-An
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326265344.33
147 rdf:type schema:Person
148 sg:person.016313412544.37 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
149 schema:familyName Kang
150 schema:givenName Hyun-Gu
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016313412544.37
152 rdf:type schema:Person
153 sg:person.0602661244.23 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
154 schema:familyName Kim
155 schema:givenName Yong-Hee
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602661244.23
157 rdf:type schema:Person
158 sg:person.0706046135.57 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
159 schema:familyName Kim
160 schema:givenName Bang-Jin
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706046135.57
162 rdf:type schema:Person
163 sg:person.0730322707.27 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
164 schema:familyName Kim
165 schema:givenName Ki-Jung
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730322707.27
167 rdf:type schema:Person
168 sg:person.07651674305.71 schema:affiliation https://www.grid.ac/institutes/grid.258676.8
169 schema:familyName Do
170 schema:givenName Jeong Tae
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07651674305.71
172 rdf:type schema:Person
173 sg:pub.10.1038/343657a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001498814
174 https://doi.org/10.1038/343657a0
175 rdf:type schema:CreativeWork
176 sg:pub.10.1038/344435a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018230461
177 https://doi.org/10.1038/344435a0
178 rdf:type schema:CreativeWork
179 sg:pub.10.1038/74199 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018147371
180 https://doi.org/10.1038/74199
181 rdf:type schema:CreativeWork
182 sg:pub.10.1038/nature04697 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014036272
183 https://doi.org/10.1038/nature04697
184 rdf:type schema:CreativeWork
185 sg:pub.10.1038/nature06129 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030896342
186 https://doi.org/10.1038/nature06129
187 rdf:type schema:CreativeWork
188 sg:pub.10.1038/nature07404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005967947
189 https://doi.org/10.1038/nature07404
190 rdf:type schema:CreativeWork
191 sg:pub.10.1038/nrm1438 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028911022
192 https://doi.org/10.1038/nrm1438
193 rdf:type schema:CreativeWork
194 sg:pub.10.1186/1471-2148-7-164 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038570894
195 https://doi.org/10.1186/1471-2148-7-164
196 rdf:type schema:CreativeWork
197 https://app.dimensions.ai/details/publication/pub.1076896194 schema:CreativeWork
198 https://app.dimensions.ai/details/publication/pub.1082886299 schema:CreativeWork
199 https://doi.org/10.1002/ar.1091690306 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046232846
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1002/dvdy.20034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041414983
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1002/stem.5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014011519
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1006/exnr.1999.7319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018311210
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/0092-8674(90)90597-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043021105
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/0092-8674(91)90573-h schema:sameAs https://app.dimensions.ai/details/publication/pub.1004492987
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/0092-8674(92)90550-v schema:sameAs https://app.dimensions.ai/details/publication/pub.1041949611
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/j.cell.2004.11.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024217468
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/j.cell.2006.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014573758
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/j.cell.2006.11.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001802823
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/j.mod.2004.08.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006327361
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/j.stem.2009.05.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039248669
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/j.ydbio.2004.07.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032636156
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/s0092-8674(03)00392-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004929245
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/s0092-8674(03)00393-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033478972
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/s0925-4773(98)00002-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004003754
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1016/s0960-9822(02)00432-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004343393
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/s1567-133x(03)00005-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014712446
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1073/pnas.0407063101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046300934
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1073/pnas.0506970102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008600171
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1073/pnas.0905657107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021649064
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1073/pnas.96.10.5504 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002967475
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1073/pnas.97.15.8346 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009438925
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1073/pnas.97.26.14720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046407009
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1089/153623003321512193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059214910
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1095/biolreprod.103.017012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002372289
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1095/biolreprod.107.066068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037408546
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1101/gad.9.17.2105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003744681
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1126/science.1082940 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050452884
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1126/science.1151526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048905674
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1128/mcb.15.1.141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062713624
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1152/physrev.1972.52.1.198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1080482667
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1182/blood-2007-02-074120 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037729391
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1530/rep-07-0479 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067716975
266 rdf:type schema:CreativeWork
267 https://www.grid.ac/institutes/grid.17088.36 schema:alternateName Michigan State University
268 schema:name Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea
269 Obstetrics, Gynecology & Reproductive Biology, Michigan State University, East Lansing, USA
270 rdf:type schema:Organization
271 https://www.grid.ac/institutes/grid.254224.7 schema:alternateName Chung-Ang University
272 schema:name Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea
273 rdf:type schema:Organization
274 https://www.grid.ac/institutes/grid.255168.d schema:alternateName Dongguk University
275 schema:name Department of Obstetrics and Gynecology, Dongguk University, Medical college Geongju, Korea
276 rdf:type schema:Organization
277 https://www.grid.ac/institutes/grid.258676.8 schema:alternateName Konkuk University
278 schema:name Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, Korea
279 rdf:type schema:Organization
280 https://www.grid.ac/institutes/grid.4280.e schema:alternateName National University of Singapore
281 schema:name Department of Animal Science & Technology, Chung-Ang University, Ansung, Korea
282 Department of Chemistry, National University of Singapore, Singapore, Republic of Singapore
283 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...