Ontology type: schema:ScholarlyArticle Open Access: True
2012-08-09
AUTHORSShilpi Paul, Madan M. Gupta, Suman P. S. Khanuja
ABSTRACTAn efficient protocol for maintaining the artemisinin content in tissue culture and high frequency of in vitro direct and indirect regenerations of multiple shoots of high artemisinin yielding genotypes of Artemisia annua has been developed and their comparison with field grown mother plant has been carried out. The eleven elite genotypes (containing more than 1% artemisinin) were tested on seven different modified media formulations. Modified half MS (Murashige and Skoog’s) media containing 100 mg L−1 myo-inositol, 0.5 g L−1 casine hydrolysate, 5 mg L−1 biotin, 2 mL L−1 RT (Revised Tobacco) vitamin stock, 0.5 mg L−1 BAP and 0.01 mg L−1 NAA showed best regeneration while, above modified MS medium containing 0.2 mg L−1 BAP and 0.2 mg L−1 NAA showed maximum shoot multiplication with maintained artemisinin content. Based on the chemical profiles of both the systems, minor difference was observed in their artemisinin content. A large scale culture of these plants maintained the normal growth index along with the artemisinin content and could be a better alternative to maintain the high artemisinin yielding genotypes with their genetic constraint in specific media combinations and also used as base material for further genetic improvement.Graphical abstract More... »
PAGES194-199
http://scigraph.springernature.com/pub.10.1007/s13659-012-0047-7
DOIhttp://dx.doi.org/10.1007/s13659-012-0047-7
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1020997047
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Plant Biology",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Central Institute of Medicinal and Aromatic Plants (CSIR), Lucknow, India",
"id": "http://www.grid.ac/institutes/grid.417631.6",
"name": [
"Central Institute of Medicinal and Aromatic Plants (CSIR), Lucknow, India"
],
"type": "Organization"
},
"familyName": "Paul",
"givenName": "Shilpi",
"id": "sg:person.0656636413.72",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656636413.72"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Central Institute of Medicinal and Aromatic Plants (CSIR), Lucknow, India",
"id": "http://www.grid.ac/institutes/grid.417631.6",
"name": [
"Central Institute of Medicinal and Aromatic Plants (CSIR), Lucknow, India"
],
"type": "Organization"
},
"familyName": "Gupta",
"givenName": "Madan M.",
"id": "sg:person.01242460441.07",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460441.07"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Central Institute of Medicinal and Aromatic Plants (CSIR), Lucknow, India",
"id": "http://www.grid.ac/institutes/grid.417631.6",
"name": [
"Central Institute of Medicinal and Aromatic Plants (CSIR), Lucknow, India"
],
"type": "Organization"
},
"familyName": "Khanuja",
"givenName": "Suman P. S.",
"id": "sg:person.010601233534.89",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010601233534.89"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/bf00029850",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012709986",
"https://doi.org/10.1007/bf00029850"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1475-2875-9-43",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029042490",
"https://doi.org/10.1186/1475-2875-9-43"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00048526",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033741604",
"https://doi.org/10.1007/bf00048526"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11627-011-9343-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032351842",
"https://doi.org/10.1007/s11627-011-9343-x"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1007528101452",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020688090",
"https://doi.org/10.1023/a:1007528101452"
],
"type": "CreativeWork"
}
],
"datePublished": "2012-08-09",
"datePublishedReg": "2012-08-09",
"description": "An efficient protocol for maintaining the artemisinin content in tissue culture and high frequency of in vitro direct and indirect regenerations of multiple shoots of high artemisinin yielding genotypes of Artemisia annua has been developed and their comparison with field grown mother plant has been carried out. The eleven elite genotypes (containing more than 1% artemisinin) were tested on seven different modified media formulations. Modified half MS (Murashige and Skoog\u2019s) media containing 100 mg L\u22121 myo-inositol, 0.5 g L\u22121 casine hydrolysate, 5 mg L\u22121 biotin, 2 mL L\u22121 RT (Revised Tobacco) vitamin stock, 0.5 mg L\u22121 BAP and 0.01 mg L\u22121 NAA showed best regeneration while, above modified MS medium containing 0.2 mg L\u22121 BAP and 0.2 mg L\u22121 NAA showed maximum shoot multiplication with maintained artemisinin content. Based on the chemical profiles of both the systems, minor difference was observed in their artemisinin content. A large scale culture of these plants maintained the normal growth index along with the artemisinin content and could be a better alternative to maintain the high artemisinin yielding genotypes with their genetic constraint in specific media combinations and also used as base material for further genetic improvement.Graphical abstract",
"genre": "article",
"id": "sg:pub.10.1007/s13659-012-0047-7",
"inLanguage": "en",
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1046357",
"issn": [
"2192-2195",
"2192-2209"
],
"name": "Natural Products and Bioprospecting",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "5",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "2"
}
],
"keywords": [
"artemisinin content",
"mother plant",
"high artemisinin",
"field grown mother plants",
"MS medium",
"half MS medium",
"further genetic improvement",
"genetic constraints",
"maximum shoot multiplication",
"genetic improvement",
"elite genotypes",
"Artemisia annua L.",
"indirect regeneration",
"multiple shoots",
"shoot multiplication",
"large-scale culture",
"plants",
"Artemisia annua",
"assessment of variation",
"tissue culture",
"scale culture",
"genotypes",
"medium formulation",
"myo-inositol",
"growth index",
"regeneration",
"BAP",
"chemical profiles",
"shoots",
"NAA",
"efficient protocol",
"annua",
"good regeneration",
"minor differences",
"media combinations",
"L.",
"culture",
"artemisinin",
"biotin",
"Graphical Abstract",
"content",
"hydrolysate",
"high frequency",
"medium",
"stocks",
"variation",
"good alternative",
"multiplication",
"profile",
"combination",
"Abstract",
"differences",
"comparison",
"index",
"alternative",
"protocol",
"system",
"improvement",
"frequency",
"constraints",
"assessment",
"field",
"formulation",
"mL",
"materials",
"base material"
],
"name": "Maintaining the artemisinin content through direct and indirect in vitro regeneration and their assessment of variations with the field grown mother plants of Artemisia annua L.",
"pagination": "194-199",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1020997047"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s13659-012-0047-7"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s13659-012-0047-7",
"https://app.dimensions.ai/details/publication/pub.1020997047"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:27",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_555.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s13659-012-0047-7"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s13659-012-0047-7'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s13659-012-0047-7'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s13659-012-0047-7'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s13659-012-0047-7'
This table displays all metadata directly associated to this object as RDF triples.
158 TRIPLES
22 PREDICATES
96 URIs
83 LITERALS
6 BLANK NODES
Subject | Predicate | Object | |
---|---|---|---|
1 | sg:pub.10.1007/s13659-012-0047-7 | schema:about | anzsrc-for:06 |
2 | ″ | ″ | anzsrc-for:0607 |
3 | ″ | schema:author | Na8a87fb5e9474bbf860cd8c236038401 |
4 | ″ | schema:citation | sg:pub.10.1007/bf00029850 |
5 | ″ | ″ | sg:pub.10.1007/bf00048526 |
6 | ″ | ″ | sg:pub.10.1007/s11627-011-9343-x |
7 | ″ | ″ | sg:pub.10.1023/a:1007528101452 |
8 | ″ | ″ | sg:pub.10.1186/1475-2875-9-43 |
9 | ″ | schema:datePublished | 2012-08-09 |
10 | ″ | schema:datePublishedReg | 2012-08-09 |
11 | ″ | schema:description | An efficient protocol for maintaining the artemisinin content in tissue culture and high frequency of in vitro direct and indirect regenerations of multiple shoots of high artemisinin yielding genotypes of Artemisia annua has been developed and their comparison with field grown mother plant has been carried out. The eleven elite genotypes (containing more than 1% artemisinin) were tested on seven different modified media formulations. Modified half MS (Murashige and Skoog’s) media containing 100 mg L−1 myo-inositol, 0.5 g L−1 casine hydrolysate, 5 mg L−1 biotin, 2 mL L−1 RT (Revised Tobacco) vitamin stock, 0.5 mg L−1 BAP and 0.01 mg L−1 NAA showed best regeneration while, above modified MS medium containing 0.2 mg L−1 BAP and 0.2 mg L−1 NAA showed maximum shoot multiplication with maintained artemisinin content. Based on the chemical profiles of both the systems, minor difference was observed in their artemisinin content. A large scale culture of these plants maintained the normal growth index along with the artemisinin content and could be a better alternative to maintain the high artemisinin yielding genotypes with their genetic constraint in specific media combinations and also used as base material for further genetic improvement.Graphical abstract |
12 | ″ | schema:genre | article |
13 | ″ | schema:inLanguage | en |
14 | ″ | schema:isAccessibleForFree | true |
15 | ″ | schema:isPartOf | N8360c69cb6834a2daae10a011186c41c |
16 | ″ | ″ | Nb58ee93bc4ce4ea99ab61376759c31ed |
17 | ″ | ″ | sg:journal.1046357 |
18 | ″ | schema:keywords | Abstract |
19 | ″ | ″ | Artemisia annua |
20 | ″ | ″ | Artemisia annua L. |
21 | ″ | ″ | BAP |
22 | ″ | ″ | Graphical Abstract |
23 | ″ | ″ | L. |
24 | ″ | ″ | MS medium |
25 | ″ | ″ | NAA |
26 | ″ | ″ | alternative |
27 | ″ | ″ | annua |
28 | ″ | ″ | artemisinin |
29 | ″ | ″ | artemisinin content |
30 | ″ | ″ | assessment |
31 | ″ | ″ | assessment of variation |
32 | ″ | ″ | base material |
33 | ″ | ″ | biotin |
34 | ″ | ″ | chemical profiles |
35 | ″ | ″ | combination |
36 | ″ | ″ | comparison |
37 | ″ | ″ | constraints |
38 | ″ | ″ | content |
39 | ″ | ″ | culture |
40 | ″ | ″ | differences |
41 | ″ | ″ | efficient protocol |
42 | ″ | ″ | elite genotypes |
43 | ″ | ″ | field |
44 | ″ | ″ | field grown mother plants |
45 | ″ | ″ | formulation |
46 | ″ | ″ | frequency |
47 | ″ | ″ | further genetic improvement |
48 | ″ | ″ | genetic constraints |
49 | ″ | ″ | genetic improvement |
50 | ″ | ″ | genotypes |
51 | ″ | ″ | good alternative |
52 | ″ | ″ | good regeneration |
53 | ″ | ″ | growth index |
54 | ″ | ″ | half MS medium |
55 | ″ | ″ | high artemisinin |
56 | ″ | ″ | high frequency |
57 | ″ | ″ | hydrolysate |
58 | ″ | ″ | improvement |
59 | ″ | ″ | index |
60 | ″ | ″ | indirect regeneration |
61 | ″ | ″ | large-scale culture |
62 | ″ | ″ | mL |
63 | ″ | ″ | materials |
64 | ″ | ″ | maximum shoot multiplication |
65 | ″ | ″ | media combinations |
66 | ″ | ″ | medium |
67 | ″ | ″ | medium formulation |
68 | ″ | ″ | minor differences |
69 | ″ | ″ | mother plant |
70 | ″ | ″ | multiple shoots |
71 | ″ | ″ | multiplication |
72 | ″ | ″ | myo-inositol |
73 | ″ | ″ | plants |
74 | ″ | ″ | profile |
75 | ″ | ″ | protocol |
76 | ″ | ″ | regeneration |
77 | ″ | ″ | scale culture |
78 | ″ | ″ | shoot multiplication |
79 | ″ | ″ | shoots |
80 | ″ | ″ | stocks |
81 | ″ | ″ | system |
82 | ″ | ″ | tissue culture |
83 | ″ | ″ | variation |
84 | ″ | schema:name | Maintaining the artemisinin content through direct and indirect in vitro regeneration and their assessment of variations with the field grown mother plants of Artemisia annua L. |
85 | ″ | schema:pagination | 194-199 |
86 | ″ | schema:productId | N264c5fc203814a6392c9eea3ed80d34e |
87 | ″ | ″ | Nec3b1a470aee4a8ea31c8f31e4a0f8a2 |
88 | ″ | schema:sameAs | https://app.dimensions.ai/details/publication/pub.1020997047 |
89 | ″ | ″ | https://doi.org/10.1007/s13659-012-0047-7 |
90 | ″ | schema:sdDatePublished | 2022-05-20T07:27 |
91 | ″ | schema:sdLicense | https://scigraph.springernature.com/explorer/license/ |
92 | ″ | schema:sdPublisher | N9dc0dc49606b4792b00b36da6b481bfb |
93 | ″ | schema:url | https://doi.org/10.1007/s13659-012-0047-7 |
94 | ″ | sgo:license | sg:explorer/license/ |
95 | ″ | sgo:sdDataset | articles |
96 | ″ | rdf:type | schema:ScholarlyArticle |
97 | N264c5fc203814a6392c9eea3ed80d34e | schema:name | dimensions_id |
98 | ″ | schema:value | pub.1020997047 |
99 | ″ | rdf:type | schema:PropertyValue |
100 | N6fd0a249625e43afb992be9ecffda40c | rdf:first | sg:person.01242460441.07 |
101 | ″ | rdf:rest | Nb1a702b9081d432999c0d3f17acbf2b4 |
102 | N8360c69cb6834a2daae10a011186c41c | schema:issueNumber | 5 |
103 | ″ | rdf:type | schema:PublicationIssue |
104 | N9dc0dc49606b4792b00b36da6b481bfb | schema:name | Springer Nature - SN SciGraph project |
105 | ″ | rdf:type | schema:Organization |
106 | Na8a87fb5e9474bbf860cd8c236038401 | rdf:first | sg:person.0656636413.72 |
107 | ″ | rdf:rest | N6fd0a249625e43afb992be9ecffda40c |
108 | Nb1a702b9081d432999c0d3f17acbf2b4 | rdf:first | sg:person.010601233534.89 |
109 | ″ | rdf:rest | rdf:nil |
110 | Nb58ee93bc4ce4ea99ab61376759c31ed | schema:volumeNumber | 2 |
111 | ″ | rdf:type | schema:PublicationVolume |
112 | Nec3b1a470aee4a8ea31c8f31e4a0f8a2 | schema:name | doi |
113 | ″ | schema:value | 10.1007/s13659-012-0047-7 |
114 | ″ | rdf:type | schema:PropertyValue |
115 | anzsrc-for:06 | schema:inDefinedTermSet | anzsrc-for: |
116 | ″ | schema:name | Biological Sciences |
117 | ″ | rdf:type | schema:DefinedTerm |
118 | anzsrc-for:0607 | schema:inDefinedTermSet | anzsrc-for: |
119 | ″ | schema:name | Plant Biology |
120 | ″ | rdf:type | schema:DefinedTerm |
121 | sg:journal.1046357 | schema:issn | 2192-2195 |
122 | ″ | ″ | 2192-2209 |
123 | ″ | schema:name | Natural Products and Bioprospecting |
124 | ″ | schema:publisher | Springer Nature |
125 | ″ | rdf:type | schema:Periodical |
126 | sg:person.010601233534.89 | schema:affiliation | grid-institutes:grid.417631.6 |
127 | ″ | schema:familyName | Khanuja |
128 | ″ | schema:givenName | Suman P. S. |
129 | ″ | schema:sameAs | https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010601233534.89 |
130 | ″ | rdf:type | schema:Person |
131 | sg:person.01242460441.07 | schema:affiliation | grid-institutes:grid.417631.6 |
132 | ″ | schema:familyName | Gupta |
133 | ″ | schema:givenName | Madan M. |
134 | ″ | schema:sameAs | https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460441.07 |
135 | ″ | rdf:type | schema:Person |
136 | sg:person.0656636413.72 | schema:affiliation | grid-institutes:grid.417631.6 |
137 | ″ | schema:familyName | Paul |
138 | ″ | schema:givenName | Shilpi |
139 | ″ | schema:sameAs | https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656636413.72 |
140 | ″ | rdf:type | schema:Person |
141 | sg:pub.10.1007/bf00029850 | schema:sameAs | https://app.dimensions.ai/details/publication/pub.1012709986 |
142 | ″ | ″ | https://doi.org/10.1007/bf00029850 |
143 | ″ | rdf:type | schema:CreativeWork |
144 | sg:pub.10.1007/bf00048526 | schema:sameAs | https://app.dimensions.ai/details/publication/pub.1033741604 |
145 | ″ | ″ | https://doi.org/10.1007/bf00048526 |
146 | ″ | rdf:type | schema:CreativeWork |
147 | sg:pub.10.1007/s11627-011-9343-x | schema:sameAs | https://app.dimensions.ai/details/publication/pub.1032351842 |
148 | ″ | ″ | https://doi.org/10.1007/s11627-011-9343-x |
149 | ″ | rdf:type | schema:CreativeWork |
150 | sg:pub.10.1023/a:1007528101452 | schema:sameAs | https://app.dimensions.ai/details/publication/pub.1020688090 |
151 | ″ | ″ | https://doi.org/10.1023/a:1007528101452 |
152 | ″ | rdf:type | schema:CreativeWork |
153 | sg:pub.10.1186/1475-2875-9-43 | schema:sameAs | https://app.dimensions.ai/details/publication/pub.1029042490 |
154 | ″ | ″ | https://doi.org/10.1186/1475-2875-9-43 |
155 | ″ | rdf:type | schema:CreativeWork |
156 | grid-institutes:grid.417631.6 | schema:alternateName | Central Institute of Medicinal and Aromatic Plants (CSIR), Lucknow, India |
157 | ″ | schema:name | Central Institute of Medicinal and Aromatic Plants (CSIR), Lucknow, India |
158 | ″ | rdf:type | schema:Organization |