Ontology type: schema:ScholarlyArticle
2011-07-30
AUTHORSPratibha Misra, Dibya Dolridge Toppo, Manoj Kumar Mishra, Syed Saema, Gaurav Singh
ABSTRACTIn the present study, Agrobacterium tumefaciens-mediated transformation protocol has been optimized for Jatropha curcas—an important biofuel plant using leaf segments (LS) and hypocotyl segments (HS) from in vitro-seedlings. Among different accessions used, ‘GJ-Ran-C2’ was selected as the best for transformation. A. tumefaciens strain EHA101, containing the binary vector pIG121Hm was used for transformation, along with the GUS reporter gene containing intron and CaMV 35S as promoter. Various parameters were optimized to get the highest transient transformation expression (TTE). Augmentin® was used in the medium to control A. tumefaciens. Selection of transgenic shoots was done in the presence of kanamycin. Integration of GUS gene was confirmed by PCR analysis, whereas the expression of GUS transgene was confirmed by RT-PCR, using actin gene as internal control. GUS histochemical analysis of explants just after cocultivation showed blue color in approximately all the LS and HS but stable transformation efficiency was 5% in case of LS, whereas 4% in HS. More... »
PAGES128-133
http://scigraph.springernature.com/pub.10.1007/s13562-011-0072-3
DOIhttp://dx.doi.org/10.1007/s13562-011-0072-3
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1023162600
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Technology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1001",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Agricultural Biotechnology",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow, India",
"id": "http://www.grid.ac/institutes/grid.417642.2",
"name": [
"National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow, India"
],
"type": "Organization"
},
"familyName": "Misra",
"givenName": "Pratibha",
"id": "sg:person.01260725315.01",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260725315.01"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow, India",
"id": "http://www.grid.ac/institutes/grid.417642.2",
"name": [
"National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow, India"
],
"type": "Organization"
},
"familyName": "Toppo",
"givenName": "Dibya Dolridge",
"id": "sg:person.010133134726.67",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010133134726.67"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow, India",
"id": "http://www.grid.ac/institutes/grid.417642.2",
"name": [
"National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow, India"
],
"type": "Organization"
},
"familyName": "Mishra",
"givenName": "Manoj Kumar",
"id": "sg:person.015462507513.58",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015462507513.58"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow, India",
"id": "http://www.grid.ac/institutes/grid.417642.2",
"name": [
"National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow, India"
],
"type": "Organization"
},
"familyName": "Saema",
"givenName": "Syed",
"id": "sg:person.014165422346.03",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014165422346.03"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow, India",
"id": "http://www.grid.ac/institutes/grid.417642.2",
"name": [
"National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow, India"
],
"type": "Organization"
},
"familyName": "Singh",
"givenName": "Gaurav",
"id": "sg:person.0712215217.38",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0712215217.38"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s00299-010-0900-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018506287",
"https://doi.org/10.1007/s00299-010-0900-2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11816-008-0042-y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012983254",
"https://doi.org/10.1007/s11816-008-0042-y"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11240-005-0416-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036877569",
"https://doi.org/10.1007/s11240-005-0416-6"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11240-007-9320-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024386320",
"https://doi.org/10.1007/s11240-007-9320-6"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10725-005-0859-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011397396",
"https://doi.org/10.1007/s10725-005-0859-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11240-009-9636-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049296655",
"https://doi.org/10.1007/s11240-009-9636-5"
],
"type": "CreativeWork"
}
],
"datePublished": "2011-07-30",
"datePublishedReg": "2011-07-30",
"description": "In the present study, Agrobacterium tumefaciens-mediated transformation protocol has been optimized for Jatropha curcas\u2014an important biofuel plant using leaf segments (LS) and hypocotyl segments (HS) from in vitro-seedlings. Among different accessions used, \u2018GJ-Ran-C2\u2019 was selected as the best for transformation. A. tumefaciens strain EHA101, containing the binary vector pIG121Hm was used for transformation, along with the GUS reporter gene containing intron and CaMV 35S as promoter. Various parameters were optimized to get the highest transient transformation expression (TTE). Augmentin\u00ae was used in the medium to control A. tumefaciens. Selection of transgenic shoots was done in the presence of kanamycin. Integration of GUS gene was confirmed by PCR analysis, whereas the expression of GUS transgene was confirmed by RT-PCR, using actin gene as internal control. GUS histochemical analysis of explants just after cocultivation showed blue color in approximately all the LS and HS but stable transformation efficiency was 5% in case of LS, whereas 4% in HS.",
"genre": "article",
"id": "sg:pub.10.1007/s13562-011-0072-3",
"inLanguage": "en",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1114927",
"issn": [
"0971-7811",
"0974-1275"
],
"name": "Journal of Plant Biochemistry and Biotechnology",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "21"
}
],
"keywords": [
"Agrobacterium tumefaciens-mediated transformation protocol",
"transformation protocol",
"GUS histochemical analysis",
"binary vector pIG121Hm",
"stable transformation efficiency",
"presence of kanamycin",
"A. tumefaciens",
"transgenic shoots",
"transformation efficiency",
"GUS transgene",
"GUS gene",
"CaMV 35S",
"GUS reporter gene",
"tumefaciens",
"hypocotyl segments",
"biofuel plants",
"protocol",
"EHA101",
"pIG121Hm",
"reporter gene",
"blue color",
"leaf segments",
"transgene",
"important biofuel plant",
"Jatropha curcas L.",
"PCR analysis",
"efficiency",
"actin gene",
"cocultivation",
"kanamycin",
"different accessions",
"integration",
"genes",
"internal control",
"Jatropha curcas",
"histochemical analysis",
"RT-PCR",
"transformation",
"expression",
"introns",
"medium",
"promoter",
"seedlings",
"accessions",
"shoots",
"plants",
"leaves",
"curcas",
"explants",
"L.",
"parameters",
"selection",
"presence",
"analysis",
"color",
"segments",
"present study",
"control",
"C2",
"study",
"Augmentin",
"cases of LS",
"cases",
"transformation expressions"
],
"name": "Agrobacterium tumefaciens-mediated transformation protocol of Jatropha curcas L. using leaf and hypocotyl segments",
"pagination": "128-133",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1023162600"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s13562-011-0072-3"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s13562-011-0072-3",
"https://app.dimensions.ai/details/publication/pub.1023162600"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:27",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_550.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s13562-011-0072-3"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s13562-011-0072-3'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s13562-011-0072-3'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s13562-011-0072-3'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s13562-011-0072-3'
This table displays all metadata directly associated to this object as RDF triples.
174 TRIPLES
22 PREDICATES
95 URIs
81 LITERALS
6 BLANK NODES