Structural Characterization and Disulfide Assignment of Spider Peptide Phα1β by Mass Spectrometry View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-05

AUTHORS

Kelly L. Wormwood, Armand Gatien Ngounou Wetie, Marcus Vinicius Gomez, Yue Ju, Paul Kowalski, Marius Mihasan, Costel C. Darie

ABSTRACT

Native Phα1β is a peptide purified from the venom of the armed spider Phoneutria nigriventer that has been shown to have an extensive analgesic effect with fewer side effects than ω-conotoxin MVIIA. Recombinant Phα1β mimics the effects of the native Phα1β. Because of this, it has been suggested that Phα1β may have potential to be used as a therapeutic for controlling persistent pathological pain. The amino acid sequence of Phα1β is known; however, the exact structure and disulfide arrangement has yet to be determined. Determination of the disulfide linkages and exact structure could greatly assist in pharmacological analysis and determination of why this peptide is such an effective analgesic. Here, we used biochemical and mass spectrometry approaches to determine the disulfide linkages present in the recombinant Phα1β peptide. Using a combination of MALDI-MS, direct infusion ESI-MS, and nanoLC-MS/MS analysis of the undigested recombinant Phα1β peptide and digested with AspN, trypsin, or AspN/trypsin, we were able to identify and confirm all six disulfide linkages present in the peptide as Cys1-2, Cys3-4, Cys5-6, Cys7-8, Cys9-10, and Cys11-12. These results were also partially confirmed in the native Phα1β peptide. These experiments provide essential structural information about Phα1β and may assist in providing insight into the peptide's analgesic effect with very low side effects. Graphical Abstract ᅟ. More... »

PAGES

827-841

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13361-018-1904-3

DOI

http://dx.doi.org/10.1007/s13361-018-1904-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1103461055

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29663255


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1115", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Pharmacology and Pharmaceutical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Clarkson University", 
          "id": "https://www.grid.ac/institutes/grid.254280.9", 
          "name": [
            "Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Ave., Box 5810, 13699, Potsdam, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wormwood", 
        "givenName": "Kelly L.", 
        "id": "sg:person.01172461122.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172461122.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Clarkson University", 
          "id": "https://www.grid.ac/institutes/grid.254280.9", 
          "name": [
            "Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Ave., Box 5810, 13699, Potsdam, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ngounou Wetie", 
        "givenName": "Armand Gatien", 
        "id": "sg:person.01124345722.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124345722.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Education and Research Santa Casa Belo Horizonte-Laboratory of Toxins, Rua Domingos Vieira 590, 30150-240, Belo Horizonte, Minas Gerais, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gomez", 
        "givenName": "Marcus Vinicius", 
        "id": "sg:person.01216302035.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216302035.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Bruker Daltonics, 40 Manning Road Manning Park, 01821, Billerica, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ju", 
        "givenName": "Yue", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Bruker Daltonics, 40 Manning Road Manning Park, 01821, Billerica, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kowalski", 
        "givenName": "Paul", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Alexandru Ioan Cuza University", 
          "id": "https://www.grid.ac/institutes/grid.8168.7", 
          "name": [
            "Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Ave., Box 5810, 13699, Potsdam, NY, USA", 
            "Department of Biology, Alexandru Ioan Cuza University of Iasi, Carol I Bvd. no 11, 700506, Iasi, Romania"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mihasan", 
        "givenName": "Marius", 
        "id": "sg:person.01023041231.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023041231.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Clarkson University", 
          "id": "https://www.grid.ac/institutes/grid.254280.9", 
          "name": [
            "Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Ave., Box 5810, 13699, Potsdam, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Darie", 
        "givenName": "Costel C.", 
        "id": "sg:person.0755276466.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755276466.88"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1177/2211068212454737", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005160089"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/2211068212454737", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005160089"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pro.3101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006666561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m506709200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012095634"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07599", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015034705", 
          "https://doi.org/10.1038/nature07599"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/bti125", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015432789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/jpet.105.087023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016849510"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2010.09.041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018954185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/mcp.m700387-mcp200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019948522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pmic.201000819", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021582280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.14.5523", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030352384"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2006.03.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032233091"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/cas.12209", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035152688"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1477-5956-10-47", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037357475", 
          "https://doi.org/10.1186/1477-5956-10-47"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1984.tb08428.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039793600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pain.2008.07.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041795450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pain.2008.07.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041795450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4103/0250-474x.102537", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043718100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.toxicon.2008.05.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043963287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btp033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044035298"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m304026200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045769798"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1110/ps.072819107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047566097"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0041-0101(93)90354-l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052446719"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0041-0101(93)90354-l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052446719"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/elps.201200182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052592948"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.74.082803.133039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052930254"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.74.082803.133039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052930254"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi048958k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055199464"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi048958k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055199464"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0495937", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055199850"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0495937", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055199850"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi049647c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055199886"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi049647c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055199886"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/138920306777452312", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069179120"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.toxicon.2017.05.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085420029"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-05", 
    "datePublishedReg": "2018-05-01", 
    "description": "Native Ph\u03b11\u03b2 is a peptide purified from the venom of the armed spider Phoneutria nigriventer that has been shown to have an extensive analgesic effect with fewer side effects than \u03c9-conotoxin MVIIA. Recombinant Ph\u03b11\u03b2 mimics the effects of the native Ph\u03b11\u03b2. Because of this, it has been suggested that Ph\u03b11\u03b2 may have potential to be used as a therapeutic for controlling persistent pathological pain. The amino acid sequence of Ph\u03b11\u03b2 is known; however, the exact structure and disulfide arrangement has yet to be determined. Determination of the disulfide linkages and exact structure could greatly assist in pharmacological analysis and determination of why this peptide is such an effective analgesic. Here, we used biochemical and mass spectrometry approaches to determine the disulfide linkages present in the recombinant Ph\u03b11\u03b2 peptide. Using a combination of MALDI-MS, direct infusion ESI-MS, and nanoLC-MS/MS analysis of the undigested recombinant Ph\u03b11\u03b2 peptide and digested with AspN, trypsin, or AspN/trypsin, we were able to identify and confirm all six disulfide linkages present in the peptide as Cys1-2, Cys3-4, Cys5-6, Cys7-8, Cys9-10, and Cys11-12. These results were also partially confirmed in the native Ph\u03b11\u03b2 peptide. These experiments provide essential structural information about Ph\u03b11\u03b2 and may assist in providing insight into the peptide's analgesic effect with very low side effects. Graphical Abstract \u115f.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s13361-018-1904-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1100508", 
        "issn": [
          "1044-0305", 
          "1879-1123"
        ], 
        "name": "Journal of The American Society for Mass Spectrometry", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "29"
      }
    ], 
    "name": "Structural Characterization and Disulfide Assignment of Spider Peptide Ph\u03b11\u03b2 by Mass Spectrometry", 
    "pagination": "827-841", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "5caf762882f07e01a5d0674b3a4e4d62aacf8a5f8ae6b5f890c4068778b5b2fb"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29663255"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9010412"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s13361-018-1904-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1103461055"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s13361-018-1904-3", 
      "https://app.dimensions.ai/details/publication/pub.1103461055"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:51", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89789_00000003.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs13361-018-1904-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s13361-018-1904-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s13361-018-1904-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s13361-018-1904-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s13361-018-1904-3'


 

This table displays all metadata directly associated to this object as RDF triples.

205 TRIPLES      21 PREDICATES      57 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s13361-018-1904-3 schema:about anzsrc-for:11
2 anzsrc-for:1115
3 schema:author N337eaa728b9842d08e8acb0750649525
4 schema:citation sg:pub.10.1038/nature07599
5 sg:pub.10.1186/1477-5956-10-47
6 https://doi.org/10.1002/elps.201200182
7 https://doi.org/10.1002/pmic.201000819
8 https://doi.org/10.1002/pro.3101
9 https://doi.org/10.1016/0041-0101(93)90354-l
10 https://doi.org/10.1016/j.cell.2010.09.041
11 https://doi.org/10.1016/j.jmb.2006.03.017
12 https://doi.org/10.1016/j.pain.2008.07.014
13 https://doi.org/10.1016/j.toxicon.2008.05.019
14 https://doi.org/10.1016/j.toxicon.2017.05.018
15 https://doi.org/10.1021/bi048958k
16 https://doi.org/10.1021/bi0495937
17 https://doi.org/10.1021/bi049647c
18 https://doi.org/10.1073/pnas.87.14.5523
19 https://doi.org/10.1074/jbc.m304026200
20 https://doi.org/10.1074/jbc.m506709200
21 https://doi.org/10.1074/mcp.m700387-mcp200
22 https://doi.org/10.1093/bioinformatics/bti125
23 https://doi.org/10.1093/bioinformatics/btp033
24 https://doi.org/10.1110/ps.072819107
25 https://doi.org/10.1111/cas.12209
26 https://doi.org/10.1111/j.1432-1033.1984.tb08428.x
27 https://doi.org/10.1124/jpet.105.087023
28 https://doi.org/10.1146/annurev.biochem.74.082803.133039
29 https://doi.org/10.1177/2211068212454737
30 https://doi.org/10.2174/138920306777452312
31 https://doi.org/10.4103/0250-474x.102537
32 schema:datePublished 2018-05
33 schema:datePublishedReg 2018-05-01
34 schema:description Native Phα1β is a peptide purified from the venom of the armed spider Phoneutria nigriventer that has been shown to have an extensive analgesic effect with fewer side effects than ω-conotoxin MVIIA. Recombinant Phα1β mimics the effects of the native Phα1β. Because of this, it has been suggested that Phα1β may have potential to be used as a therapeutic for controlling persistent pathological pain. The amino acid sequence of Phα1β is known; however, the exact structure and disulfide arrangement has yet to be determined. Determination of the disulfide linkages and exact structure could greatly assist in pharmacological analysis and determination of why this peptide is such an effective analgesic. Here, we used biochemical and mass spectrometry approaches to determine the disulfide linkages present in the recombinant Phα1β peptide. Using a combination of MALDI-MS, direct infusion ESI-MS, and nanoLC-MS/MS analysis of the undigested recombinant Phα1β peptide and digested with AspN, trypsin, or AspN/trypsin, we were able to identify and confirm all six disulfide linkages present in the peptide as Cys1-2, Cys3-4, Cys5-6, Cys7-8, Cys9-10, and Cys11-12. These results were also partially confirmed in the native Phα1β peptide. These experiments provide essential structural information about Phα1β and may assist in providing insight into the peptide's analgesic effect with very low side effects. Graphical Abstract ᅟ.
35 schema:genre research_article
36 schema:inLanguage en
37 schema:isAccessibleForFree false
38 schema:isPartOf N45e4ca94be2e40e793d99a158cbb46cb
39 N6c8cc1e4f93243ee848d43bf63374c12
40 sg:journal.1100508
41 schema:name Structural Characterization and Disulfide Assignment of Spider Peptide Phα1β by Mass Spectrometry
42 schema:pagination 827-841
43 schema:productId N4c154c8f55d14753a19c4bacff7e59cb
44 N5471c0ee56854d2b9481cd1d4e2b69e1
45 Na800ad12423f4fcd88fd70ec528ca0de
46 Ndee7419fedb84ba7badc3c114d27c3f3
47 Nf6f261c9d1a24f8ba520cc20415ea7b2
48 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103461055
49 https://doi.org/10.1007/s13361-018-1904-3
50 schema:sdDatePublished 2019-04-11T09:51
51 schema:sdLicense https://scigraph.springernature.com/explorer/license/
52 schema:sdPublisher Ne26e7b552b5c4f889605d912c3d0b2c7
53 schema:url https://link.springer.com/10.1007%2Fs13361-018-1904-3
54 sgo:license sg:explorer/license/
55 sgo:sdDataset articles
56 rdf:type schema:ScholarlyArticle
57 N337eaa728b9842d08e8acb0750649525 rdf:first sg:person.01172461122.43
58 rdf:rest N768ea4e4d86240aab91e6fdeddf44b22
59 N36224232c7044f55b46c875827b90d1e schema:affiliation N42544007a3ff4f58a459d86dd94f0135
60 schema:familyName Kowalski
61 schema:givenName Paul
62 rdf:type schema:Person
63 N36a2c823caf74bbe8f73e081c7595146 rdf:first N36224232c7044f55b46c875827b90d1e
64 rdf:rest Na48185888b4e4271a4ead2870b327d2e
65 N42544007a3ff4f58a459d86dd94f0135 schema:name Bruker Daltonics, 40 Manning Road Manning Park, 01821, Billerica, MA, USA
66 rdf:type schema:Organization
67 N45e4ca94be2e40e793d99a158cbb46cb schema:volumeNumber 29
68 rdf:type schema:PublicationVolume
69 N4c154c8f55d14753a19c4bacff7e59cb schema:name doi
70 schema:value 10.1007/s13361-018-1904-3
71 rdf:type schema:PropertyValue
72 N5471c0ee56854d2b9481cd1d4e2b69e1 schema:name readcube_id
73 schema:value 5caf762882f07e01a5d0674b3a4e4d62aacf8a5f8ae6b5f890c4068778b5b2fb
74 rdf:type schema:PropertyValue
75 N5cbcaad4d6314f969dd5802bd12422e0 schema:affiliation N742aea33f5474e2c88cbdaa79f7f9a92
76 schema:familyName Ju
77 schema:givenName Yue
78 rdf:type schema:Person
79 N6c8cc1e4f93243ee848d43bf63374c12 schema:issueNumber 5
80 rdf:type schema:PublicationIssue
81 N742aea33f5474e2c88cbdaa79f7f9a92 schema:name Bruker Daltonics, 40 Manning Road Manning Park, 01821, Billerica, MA, USA
82 rdf:type schema:Organization
83 N768ea4e4d86240aab91e6fdeddf44b22 rdf:first sg:person.01124345722.84
84 rdf:rest N85561c7a3c484bb0afcb4aa5aba7e051
85 N8130752ccb6a4d1b92718cf2de176a39 rdf:first sg:person.0755276466.88
86 rdf:rest rdf:nil
87 N85561c7a3c484bb0afcb4aa5aba7e051 rdf:first sg:person.01216302035.42
88 rdf:rest Na86d9410a02e4964be3a35e97a238185
89 Na48185888b4e4271a4ead2870b327d2e rdf:first sg:person.01023041231.56
90 rdf:rest N8130752ccb6a4d1b92718cf2de176a39
91 Na800ad12423f4fcd88fd70ec528ca0de schema:name pubmed_id
92 schema:value 29663255
93 rdf:type schema:PropertyValue
94 Na86d9410a02e4964be3a35e97a238185 rdf:first N5cbcaad4d6314f969dd5802bd12422e0
95 rdf:rest N36a2c823caf74bbe8f73e081c7595146
96 Ndee7419fedb84ba7badc3c114d27c3f3 schema:name nlm_unique_id
97 schema:value 9010412
98 rdf:type schema:PropertyValue
99 Ne26e7b552b5c4f889605d912c3d0b2c7 schema:name Springer Nature - SN SciGraph project
100 rdf:type schema:Organization
101 Nf09614440c1d41769b074efdf7e84b07 schema:name Institute of Education and Research Santa Casa Belo Horizonte-Laboratory of Toxins, Rua Domingos Vieira 590, 30150-240, Belo Horizonte, Minas Gerais, Brazil
102 rdf:type schema:Organization
103 Nf6f261c9d1a24f8ba520cc20415ea7b2 schema:name dimensions_id
104 schema:value pub.1103461055
105 rdf:type schema:PropertyValue
106 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
107 schema:name Medical and Health Sciences
108 rdf:type schema:DefinedTerm
109 anzsrc-for:1115 schema:inDefinedTermSet anzsrc-for:
110 schema:name Pharmacology and Pharmaceutical Sciences
111 rdf:type schema:DefinedTerm
112 sg:journal.1100508 schema:issn 1044-0305
113 1879-1123
114 schema:name Journal of The American Society for Mass Spectrometry
115 rdf:type schema:Periodical
116 sg:person.01023041231.56 schema:affiliation https://www.grid.ac/institutes/grid.8168.7
117 schema:familyName Mihasan
118 schema:givenName Marius
119 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023041231.56
120 rdf:type schema:Person
121 sg:person.01124345722.84 schema:affiliation https://www.grid.ac/institutes/grid.254280.9
122 schema:familyName Ngounou Wetie
123 schema:givenName Armand Gatien
124 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124345722.84
125 rdf:type schema:Person
126 sg:person.01172461122.43 schema:affiliation https://www.grid.ac/institutes/grid.254280.9
127 schema:familyName Wormwood
128 schema:givenName Kelly L.
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172461122.43
130 rdf:type schema:Person
131 sg:person.01216302035.42 schema:affiliation Nf09614440c1d41769b074efdf7e84b07
132 schema:familyName Gomez
133 schema:givenName Marcus Vinicius
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216302035.42
135 rdf:type schema:Person
136 sg:person.0755276466.88 schema:affiliation https://www.grid.ac/institutes/grid.254280.9
137 schema:familyName Darie
138 schema:givenName Costel C.
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755276466.88
140 rdf:type schema:Person
141 sg:pub.10.1038/nature07599 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015034705
142 https://doi.org/10.1038/nature07599
143 rdf:type schema:CreativeWork
144 sg:pub.10.1186/1477-5956-10-47 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037357475
145 https://doi.org/10.1186/1477-5956-10-47
146 rdf:type schema:CreativeWork
147 https://doi.org/10.1002/elps.201200182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052592948
148 rdf:type schema:CreativeWork
149 https://doi.org/10.1002/pmic.201000819 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021582280
150 rdf:type schema:CreativeWork
151 https://doi.org/10.1002/pro.3101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006666561
152 rdf:type schema:CreativeWork
153 https://doi.org/10.1016/0041-0101(93)90354-l schema:sameAs https://app.dimensions.ai/details/publication/pub.1052446719
154 rdf:type schema:CreativeWork
155 https://doi.org/10.1016/j.cell.2010.09.041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018954185
156 rdf:type schema:CreativeWork
157 https://doi.org/10.1016/j.jmb.2006.03.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032233091
158 rdf:type schema:CreativeWork
159 https://doi.org/10.1016/j.pain.2008.07.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041795450
160 rdf:type schema:CreativeWork
161 https://doi.org/10.1016/j.toxicon.2008.05.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043963287
162 rdf:type schema:CreativeWork
163 https://doi.org/10.1016/j.toxicon.2017.05.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085420029
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1021/bi048958k schema:sameAs https://app.dimensions.ai/details/publication/pub.1055199464
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1021/bi0495937 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055199850
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1021/bi049647c schema:sameAs https://app.dimensions.ai/details/publication/pub.1055199886
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1073/pnas.87.14.5523 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030352384
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1074/jbc.m304026200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045769798
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1074/jbc.m506709200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012095634
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1074/mcp.m700387-mcp200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019948522
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1093/bioinformatics/bti125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015432789
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1093/bioinformatics/btp033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044035298
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1110/ps.072819107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047566097
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1111/cas.12209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035152688
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1111/j.1432-1033.1984.tb08428.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1039793600
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1124/jpet.105.087023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016849510
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1146/annurev.biochem.74.082803.133039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052930254
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1177/2211068212454737 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005160089
194 rdf:type schema:CreativeWork
195 https://doi.org/10.2174/138920306777452312 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069179120
196 rdf:type schema:CreativeWork
197 https://doi.org/10.4103/0250-474x.102537 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043718100
198 rdf:type schema:CreativeWork
199 https://www.grid.ac/institutes/grid.254280.9 schema:alternateName Clarkson University
200 schema:name Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Ave., Box 5810, 13699, Potsdam, NY, USA
201 rdf:type schema:Organization
202 https://www.grid.ac/institutes/grid.8168.7 schema:alternateName Alexandru Ioan Cuza University
203 schema:name Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Ave., Box 5810, 13699, Potsdam, NY, USA
204 Department of Biology, Alexandru Ioan Cuza University of Iasi, Carol I Bvd. no 11, 700506, Iasi, Romania
205 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...