De Novo Correction of Mass Measurement Error in Low Resolution Tandem MS Spectra for Shotgun Proteomics View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012-09-25

AUTHORS

Jarrett D. Egertson, Jimmy K. Eng, Michael S. Bereman, Edward J. Hsieh, Gennifer E. Merrihew, Michael J. MacCoss

ABSTRACT

We report an algorithm designed for the calibration of low resolution peptide mass spectra. Our algorithm is implemented in a program called FineTune, which corrects systematic mass measurement error in 1 min, with no input required besides the mass spectra themselves. The mass measurement accuracy for a set of spectra collected on an LTQ-Velos improved 20-fold from –0.1776 ± 0.0010 m/z to 0.0078 ± 0.0006 m/z after calibration (avg ± 95 % confidence interval). The precision in mass measurement was improved due to the correction of non-linear variation in mass measurement accuracy across the m/z range. More... »

PAGES

2075-2082

References to SciGraph publications

  • 2002-10. Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry in NATURE
  • 2003-09-01. Proteome analyses using accurate mass and elution time peptide tags with capillary LC time-of-flight mass spectrometry in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 2008-11-30. MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification in NATURE BIOTECHNOLOGY
  • 2000-05-01. Obtaining more accurate Fourier transform ion cyclotron resonance mass measurements without internal standards using multiply charged ions in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 2005-04-01. Improved mass accuracy for tandem mass spectrometry in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 1996-11-01. Search of sequence databases with uninterpreted high-energy collision-induced dissociation spectra of peptides in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 2007-10-21. Semi-supervised learning for peptide identification from shotgun proteomics datasets in NATURE METHODS
  • 2006-09-23. Analytical model of peptide mass cluster centres with applications in PROTEOME SCIENCE
  • 1994-11-01. An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 2000-10-01. A dual electrospray ionization source combined with hexapole accumulation to achieve high mass accuracy of biopolymers in Fourier transform ion cyclotron resonance mass spectrometry in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s13361-012-0482-z

    DOI

    http://dx.doi.org/10.1007/s13361-012-0482-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1049121323

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23007965


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