Expression of human growth hormone in the milk of transgenic rabbits with transgene mapped to the telomere region of chromosome ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012-08-17

AUTHORS

Daniel Lipinski, Joanna Zeyland, Marlena Szalata, Andrzej Plawski, Malgorzata Jarmuz, Jacek Jura, Aleksandra Korcz, Zdzislaw Smorag, Marek Pienkowski, Ryszard Slomski

ABSTRACT

The advent of transgenic technology has provided methods for the production of pharmaceuticals by the isolation of these proteins from transgenic animals. The mammary gland has been focused on as a bioreactor, since milk is easily collected from lactating animals and protein production can be expressed at very high levels, including hormones and enzymes. We demonstrate here the expression pattern of recombinant human growth hormone (rhGH) in transgenic rabbits carrying hGH genomic sequences driven by the rat whey acidic protein (WAP) promoter. The transgene was mapped to the q26-27 telomere region of chromosome 7q by fluorescence in situ hybridization (FISH). Nearly 30 % of the F1 generation demonstrated the presence of transgene. The recombinant growth hormone was detected in the milk of the transgenic rabbit females, but not in serum, up to the level of 10 μg/ml. Ectopic expression of the transgene in the brain, heart, kidney, liver, and salivary gland was not observed, indicating that a short sequence of rat WAP promoter (969 bp) contained essential sequences directing expression exclusively to the mammary gland. The biological activity of recombinant growth hormone was measured by immunoreactivity and the capability to stimulate growth of the hormone-dependent Nb211 cell line. More... »

PAGES

435-442

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13353-012-0110-4

DOI

http://dx.doi.org/10.1007/s13353-012-0110-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000428364

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22898896


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals, Genetically Modified", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosome Mapping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomes, Mammalian", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Human Growth Hormone", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunoradiometric Assay", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Situ Hybridization, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mammary Glands, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Milk", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Milk Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rabbits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Telomere", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transgenes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland", 
          "id": "http://www.grid.ac/institutes/grid.420230.7", 
          "name": [
            "Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland", 
            "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lipinski", 
        "givenName": "Daniel", 
        "id": "sg:person.01051357313.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051357313.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland", 
          "id": "http://www.grid.ac/institutes/grid.410688.3", 
          "name": [
            "Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zeyland", 
        "givenName": "Joanna", 
        "id": "sg:person.0110175064.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0110175064.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland", 
          "id": "http://www.grid.ac/institutes/grid.420230.7", 
          "name": [
            "Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland", 
            "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Szalata", 
        "givenName": "Marlena", 
        "id": "sg:person.01312761504.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312761504.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland", 
          "id": "http://www.grid.ac/institutes/grid.420230.7", 
          "name": [
            "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Plawski", 
        "givenName": "Andrzej", 
        "id": "sg:person.0622456075.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622456075.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland", 
          "id": "http://www.grid.ac/institutes/grid.420230.7", 
          "name": [
            "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jarmuz", 
        "givenName": "Malgorzata", 
        "id": "sg:person.0643104657.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643104657.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Animal Reproduction, National Research Institute of Animal Production, 32-083 Balice, Poland", 
          "id": "http://www.grid.ac/institutes/grid.419741.e", 
          "name": [
            "Department of Animal Reproduction, National Research Institute of Animal Production, 32-083 Balice, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jura", 
        "givenName": "Jacek", 
        "id": "sg:person.01011740643.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011740643.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland", 
          "id": "http://www.grid.ac/institutes/grid.420230.7", 
          "name": [
            "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Korcz", 
        "givenName": "Aleksandra", 
        "id": "sg:person.0763153744.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0763153744.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Animal Reproduction, National Research Institute of Animal Production, 32-083 Balice, Poland", 
          "id": "http://www.grid.ac/institutes/grid.419741.e", 
          "name": [
            "Department of Animal Reproduction, National Research Institute of Animal Production, 32-083 Balice, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Smorag", 
        "givenName": "Zdzislaw", 
        "id": "sg:person.01113366222.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113366222.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pien Gen Biomedical Corporation, Knoxville, TN USA", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Pien Gen Biomedical Corporation, Knoxville, TN USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pienkowski", 
        "givenName": "Marek", 
        "id": "sg:person.0577414666.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577414666.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland", 
          "id": "http://www.grid.ac/institutes/grid.420230.7", 
          "name": [
            "Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland", 
            "Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Slomski", 
        "givenName": "Ryszard", 
        "id": "sg:person.01245575772.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245575772.91"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf01974085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005704362", 
          "https://doi.org/10.1007/bf01974085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0502-484", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009100839", 
          "https://doi.org/10.1038/nbt0502-484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1018723712996", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036999619", 
          "https://doi.org/10.1023/a:1018723712996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/15067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047246568", 
          "https://doi.org/10.1038/15067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1008934912555", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050836022", 
          "https://doi.org/10.1023/a:1008934912555"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1008999622117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024381800", 
          "https://doi.org/10.1023/a:1008999622117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/6158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001847991", 
          "https://doi.org/10.1038/6158"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1018406611380", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013509920", 
          "https://doi.org/10.1023/a:1018406611380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0991-835", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034255207", 
          "https://doi.org/10.1038/nbt0991-835"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-08-17", 
    "datePublishedReg": "2012-08-17", 
    "description": "The advent of transgenic technology has provided methods for the production of pharmaceuticals by the isolation of these proteins from transgenic animals. The mammary gland has been focused on as a bioreactor, since milk is easily collected from lactating animals and protein production can be expressed at very high levels, including hormones and enzymes. We demonstrate here the expression pattern of recombinant human growth hormone (rhGH) in transgenic rabbits carrying hGH genomic sequences driven by the rat whey acidic protein (WAP) promoter. The transgene was mapped to the q26-27 telomere region of chromosome 7q by fluorescence in situ hybridization (FISH). Nearly 30 % of the F1 generation demonstrated the presence of transgene. The recombinant growth hormone was detected in the milk of the transgenic rabbit females, but not in serum, up to the level of 10 \u03bcg/ml. Ectopic expression of the transgene in the brain, heart, kidney, liver, and salivary gland was not observed, indicating that a short sequence of rat WAP promoter (969 bp) contained essential sequences directing expression exclusively to the mammary gland. The biological activity of recombinant growth hormone was measured by immunoreactivity and the capability to stimulate growth of the hormone-dependent Nb211 cell line.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s13353-012-0110-4", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1114642", 
        "issn": [
          "1234-1983", 
          "2190-3883"
        ], 
        "name": "Journal of Applied Genetics", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "53"
      }
    ], 
    "keywords": [
      "telomere regions", 
      "chromosome 7q", 
      "presence of transgenes", 
      "transgenic rabbits", 
      "WAP promoter", 
      "mammary gland", 
      "genomic sequences", 
      "transgenic technology", 
      "ectopic expression", 
      "expression patterns", 
      "protein production", 
      "transgenic animals", 
      "essential sequences", 
      "protein promoter", 
      "production of pharmaceuticals", 
      "acidic protein promoter", 
      "transgene", 
      "situ hybridization", 
      "F1 generation", 
      "short sequences", 
      "cell lines", 
      "promoter", 
      "rabbit females", 
      "sequence", 
      "human growth hormone", 
      "expression", 
      "biological activity", 
      "growth hormone", 
      "salivary glands", 
      "protein", 
      "enzyme", 
      "hybridization", 
      "animals", 
      "gland", 
      "hormone", 
      "high levels", 
      "production", 
      "region", 
      "isolation", 
      "fluorescence", 
      "growth", 
      "recombinant growth hormone", 
      "levels", 
      "lines", 
      "activity", 
      "females", 
      "recombinant human growth hormone", 
      "bioreactor", 
      "patterns", 
      "presence", 
      "rabbits", 
      "brain", 
      "pharmaceuticals", 
      "milk", 
      "generation", 
      "liver", 
      "immunoreactivity", 
      "kidney", 
      "heart", 
      "advent", 
      "serum", 
      "technology", 
      "capability", 
      "rats", 
      "method", 
      "hGH genomic sequences", 
      "q26-27 telomere region", 
      "transgenic rabbit females", 
      "rat WAP promoter", 
      "hormone-dependent Nb211 cell line", 
      "Nb211 cell line"
    ], 
    "name": "Expression of human growth hormone in the milk of transgenic rabbits with transgene mapped to the telomere region of chromosome 7q", 
    "pagination": "435-442", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1000428364"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s13353-012-0110-4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22898896"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s13353-012-0110-4", 
      "https://app.dimensions.ai/details/publication/pub.1000428364"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:28", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_584.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s13353-012-0110-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s13353-012-0110-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s13353-012-0110-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s13353-012-0110-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s13353-012-0110-4'


 

This table displays all metadata directly associated to this object as RDF triples.

318 TRIPLES      22 PREDICATES      125 URIs      108 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s13353-012-0110-4 schema:about N082eede63ab1479bb6f4b5d7213839ec
2 N21e78e4d439848aa848b8eed0262dfed
3 N26c45e57e660428881d4a01fe5eb7044
4 N27db94d15ce64cecbcd11875c9b80dcc
5 N382e2d40aabf411d8670973266351961
6 N4574409fba5449d6944f146ede6e4ca4
7 N4c55ff31236b4cdcab3fd37905620d86
8 N4d83e637565b430082c6515fb3839a71
9 N507cf5cad059492e8dc699b5a21bdc7a
10 N583f650fdcfe4fe0abd5a627429c6811
11 N7bc38ccba0854e13870932d14fd443ac
12 N8aaff1e12a7e47ed893d97c4bbb2aed2
13 N95fd09782ef94ff69a8de5af104afcb9
14 N9636544511e2481d894ca6d97a8b7cf0
15 N9c4301a4f1d444828381b1b2cbdca6c0
16 N9e6b1fb407314056993459e25fe7a45d
17 Nad78e78581c044b0b50aa098e67f0e95
18 Nae1cfc31b46a47e0859d48160f3fc660
19 Nee6e39cffbe8438d832e871e2ff6502e
20 anzsrc-for:06
21 anzsrc-for:0601
22 schema:author N8f87e3ed68444a0da36657e546b44bff
23 schema:citation sg:pub.10.1007/bf01974085
24 sg:pub.10.1023/a:1008934912555
25 sg:pub.10.1023/a:1008999622117
26 sg:pub.10.1023/a:1018406611380
27 sg:pub.10.1023/a:1018723712996
28 sg:pub.10.1038/15067
29 sg:pub.10.1038/6158
30 sg:pub.10.1038/nbt0502-484
31 sg:pub.10.1038/nbt0991-835
32 schema:datePublished 2012-08-17
33 schema:datePublishedReg 2012-08-17
34 schema:description The advent of transgenic technology has provided methods for the production of pharmaceuticals by the isolation of these proteins from transgenic animals. The mammary gland has been focused on as a bioreactor, since milk is easily collected from lactating animals and protein production can be expressed at very high levels, including hormones and enzymes. We demonstrate here the expression pattern of recombinant human growth hormone (rhGH) in transgenic rabbits carrying hGH genomic sequences driven by the rat whey acidic protein (WAP) promoter. The transgene was mapped to the q26-27 telomere region of chromosome 7q by fluorescence in situ hybridization (FISH). Nearly 30 % of the F1 generation demonstrated the presence of transgene. The recombinant growth hormone was detected in the milk of the transgenic rabbit females, but not in serum, up to the level of 10 μg/ml. Ectopic expression of the transgene in the brain, heart, kidney, liver, and salivary gland was not observed, indicating that a short sequence of rat WAP promoter (969 bp) contained essential sequences directing expression exclusively to the mammary gland. The biological activity of recombinant growth hormone was measured by immunoreactivity and the capability to stimulate growth of the hormone-dependent Nb211 cell line.
35 schema:genre article
36 schema:inLanguage en
37 schema:isAccessibleForFree true
38 schema:isPartOf N3d400d035da64be98dd61a250d4d9bea
39 N4ce97e2a570e40df91e5551665e01a70
40 sg:journal.1114642
41 schema:keywords F1 generation
42 Nb211 cell line
43 WAP promoter
44 acidic protein promoter
45 activity
46 advent
47 animals
48 biological activity
49 bioreactor
50 brain
51 capability
52 cell lines
53 chromosome 7q
54 ectopic expression
55 enzyme
56 essential sequences
57 expression
58 expression patterns
59 females
60 fluorescence
61 generation
62 genomic sequences
63 gland
64 growth
65 growth hormone
66 hGH genomic sequences
67 heart
68 high levels
69 hormone
70 hormone-dependent Nb211 cell line
71 human growth hormone
72 hybridization
73 immunoreactivity
74 isolation
75 kidney
76 levels
77 lines
78 liver
79 mammary gland
80 method
81 milk
82 patterns
83 pharmaceuticals
84 presence
85 presence of transgenes
86 production
87 production of pharmaceuticals
88 promoter
89 protein
90 protein production
91 protein promoter
92 q26-27 telomere region
93 rabbit females
94 rabbits
95 rat WAP promoter
96 rats
97 recombinant growth hormone
98 recombinant human growth hormone
99 region
100 salivary glands
101 sequence
102 serum
103 short sequences
104 situ hybridization
105 technology
106 telomere regions
107 transgene
108 transgenic animals
109 transgenic rabbit females
110 transgenic rabbits
111 transgenic technology
112 schema:name Expression of human growth hormone in the milk of transgenic rabbits with transgene mapped to the telomere region of chromosome 7q
113 schema:pagination 435-442
114 schema:productId N02ad89a3a30d4085aa805e71ac18a5ce
115 N1f095089779f4cfab9d733db75df985e
116 Neabeb2e19de94e54bad06b5a7096fb86
117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000428364
118 https://doi.org/10.1007/s13353-012-0110-4
119 schema:sdDatePublished 2022-01-01T18:28
120 schema:sdLicense https://scigraph.springernature.com/explorer/license/
121 schema:sdPublisher N9f6b6586e030436d92eab964b2fe5529
122 schema:url https://doi.org/10.1007/s13353-012-0110-4
123 sgo:license sg:explorer/license/
124 sgo:sdDataset articles
125 rdf:type schema:ScholarlyArticle
126 N02ad89a3a30d4085aa805e71ac18a5ce schema:name doi
127 schema:value 10.1007/s13353-012-0110-4
128 rdf:type schema:PropertyValue
129 N082eede63ab1479bb6f4b5d7213839ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Humans
131 rdf:type schema:DefinedTerm
132 N0ebf5b36a80d42148b731b8305f5467c rdf:first sg:person.0763153744.16
133 rdf:rest Nf8ad8ec17f494957bca11ad7bfec3eb3
134 N1f095089779f4cfab9d733db75df985e schema:name dimensions_id
135 schema:value pub.1000428364
136 rdf:type schema:PropertyValue
137 N21e78e4d439848aa848b8eed0262dfed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Immunoradiometric Assay
139 rdf:type schema:DefinedTerm
140 N26c45e57e660428881d4a01fe5eb7044 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Promoter Regions, Genetic
142 rdf:type schema:DefinedTerm
143 N27db94d15ce64cecbcd11875c9b80dcc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name In Situ Hybridization, Fluorescence
145 rdf:type schema:DefinedTerm
146 N382e2d40aabf411d8670973266351961 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Rabbits
148 rdf:type schema:DefinedTerm
149 N3d400d035da64be98dd61a250d4d9bea schema:volumeNumber 53
150 rdf:type schema:PublicationVolume
151 N4574409fba5449d6944f146ede6e4ca4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Chromosome Mapping
153 rdf:type schema:DefinedTerm
154 N4c55ff31236b4cdcab3fd37905620d86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Female
156 rdf:type schema:DefinedTerm
157 N4ce97e2a570e40df91e5551665e01a70 schema:issueNumber 4
158 rdf:type schema:PublicationIssue
159 N4d83e637565b430082c6515fb3839a71 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Milk Proteins
161 rdf:type schema:DefinedTerm
162 N507cf5cad059492e8dc699b5a21bdc7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Animals, Genetically Modified
164 rdf:type schema:DefinedTerm
165 N551128e41de84e5b9cedbaa90747cf04 rdf:first sg:person.01312761504.60
166 rdf:rest Nf7ba926f05fe46089b5b61279e0f6f13
167 N583f650fdcfe4fe0abd5a627429c6811 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Milk
169 rdf:type schema:DefinedTerm
170 N5c7801fb3c684cf4950a47865983067b rdf:first sg:person.0577414666.55
171 rdf:rest Nc067181e959c492ea61f4feb6fc7d177
172 N6535ac6122334391967da81ef93cc9ec rdf:first sg:person.0643104657.75
173 rdf:rest Nd27800729f1441e4a9ec8c85c9b617bd
174 N7bc38ccba0854e13870932d14fd443ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name Human Growth Hormone
176 rdf:type schema:DefinedTerm
177 N87f216e76afe4bcd858a8d3c5ff66795 rdf:first sg:person.0110175064.01
178 rdf:rest N551128e41de84e5b9cedbaa90747cf04
179 N8aaff1e12a7e47ed893d97c4bbb2aed2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Rats
181 rdf:type schema:DefinedTerm
182 N8f87e3ed68444a0da36657e546b44bff rdf:first sg:person.01051357313.97
183 rdf:rest N87f216e76afe4bcd858a8d3c5ff66795
184 N95fd09782ef94ff69a8de5af104afcb9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name Transgenes
186 rdf:type schema:DefinedTerm
187 N9636544511e2481d894ca6d97a8b7cf0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name Telomere
189 rdf:type schema:DefinedTerm
190 N9c4301a4f1d444828381b1b2cbdca6c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name Chromosomes, Mammalian
192 rdf:type schema:DefinedTerm
193 N9e6b1fb407314056993459e25fe7a45d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
194 schema:name Gene Expression Regulation
195 rdf:type schema:DefinedTerm
196 N9f6b6586e030436d92eab964b2fe5529 schema:name Springer Nature - SN SciGraph project
197 rdf:type schema:Organization
198 Nad78e78581c044b0b50aa098e67f0e95 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
199 schema:name Mammary Glands, Animal
200 rdf:type schema:DefinedTerm
201 Nae1cfc31b46a47e0859d48160f3fc660 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
202 schema:name Animals
203 rdf:type schema:DefinedTerm
204 Nc067181e959c492ea61f4feb6fc7d177 rdf:first sg:person.01245575772.91
205 rdf:rest rdf:nil
206 Nd27800729f1441e4a9ec8c85c9b617bd rdf:first sg:person.01011740643.04
207 rdf:rest N0ebf5b36a80d42148b731b8305f5467c
208 Neabeb2e19de94e54bad06b5a7096fb86 schema:name pubmed_id
209 schema:value 22898896
210 rdf:type schema:PropertyValue
211 Nee6e39cffbe8438d832e871e2ff6502e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
212 schema:name Male
213 rdf:type schema:DefinedTerm
214 Nf7ba926f05fe46089b5b61279e0f6f13 rdf:first sg:person.0622456075.97
215 rdf:rest N6535ac6122334391967da81ef93cc9ec
216 Nf8ad8ec17f494957bca11ad7bfec3eb3 rdf:first sg:person.01113366222.95
217 rdf:rest N5c7801fb3c684cf4950a47865983067b
218 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
219 schema:name Biological Sciences
220 rdf:type schema:DefinedTerm
221 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
222 schema:name Biochemistry and Cell Biology
223 rdf:type schema:DefinedTerm
224 sg:journal.1114642 schema:issn 1234-1983
225 2190-3883
226 schema:name Journal of Applied Genetics
227 schema:publisher Springer Nature
228 rdf:type schema:Periodical
229 sg:person.01011740643.04 schema:affiliation grid-institutes:grid.419741.e
230 schema:familyName Jura
231 schema:givenName Jacek
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011740643.04
233 rdf:type schema:Person
234 sg:person.01051357313.97 schema:affiliation grid-institutes:grid.420230.7
235 schema:familyName Lipinski
236 schema:givenName Daniel
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051357313.97
238 rdf:type schema:Person
239 sg:person.0110175064.01 schema:affiliation grid-institutes:grid.410688.3
240 schema:familyName Zeyland
241 schema:givenName Joanna
242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0110175064.01
243 rdf:type schema:Person
244 sg:person.01113366222.95 schema:affiliation grid-institutes:grid.419741.e
245 schema:familyName Smorag
246 schema:givenName Zdzislaw
247 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113366222.95
248 rdf:type schema:Person
249 sg:person.01245575772.91 schema:affiliation grid-institutes:grid.420230.7
250 schema:familyName Slomski
251 schema:givenName Ryszard
252 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245575772.91
253 rdf:type schema:Person
254 sg:person.01312761504.60 schema:affiliation grid-institutes:grid.420230.7
255 schema:familyName Szalata
256 schema:givenName Marlena
257 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312761504.60
258 rdf:type schema:Person
259 sg:person.0577414666.55 schema:affiliation grid-institutes:None
260 schema:familyName Pienkowski
261 schema:givenName Marek
262 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577414666.55
263 rdf:type schema:Person
264 sg:person.0622456075.97 schema:affiliation grid-institutes:grid.420230.7
265 schema:familyName Plawski
266 schema:givenName Andrzej
267 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622456075.97
268 rdf:type schema:Person
269 sg:person.0643104657.75 schema:affiliation grid-institutes:grid.420230.7
270 schema:familyName Jarmuz
271 schema:givenName Malgorzata
272 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643104657.75
273 rdf:type schema:Person
274 sg:person.0763153744.16 schema:affiliation grid-institutes:grid.420230.7
275 schema:familyName Korcz
276 schema:givenName Aleksandra
277 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0763153744.16
278 rdf:type schema:Person
279 sg:pub.10.1007/bf01974085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005704362
280 https://doi.org/10.1007/bf01974085
281 rdf:type schema:CreativeWork
282 sg:pub.10.1023/a:1008934912555 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050836022
283 https://doi.org/10.1023/a:1008934912555
284 rdf:type schema:CreativeWork
285 sg:pub.10.1023/a:1008999622117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024381800
286 https://doi.org/10.1023/a:1008999622117
287 rdf:type schema:CreativeWork
288 sg:pub.10.1023/a:1018406611380 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013509920
289 https://doi.org/10.1023/a:1018406611380
290 rdf:type schema:CreativeWork
291 sg:pub.10.1023/a:1018723712996 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036999619
292 https://doi.org/10.1023/a:1018723712996
293 rdf:type schema:CreativeWork
294 sg:pub.10.1038/15067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047246568
295 https://doi.org/10.1038/15067
296 rdf:type schema:CreativeWork
297 sg:pub.10.1038/6158 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001847991
298 https://doi.org/10.1038/6158
299 rdf:type schema:CreativeWork
300 sg:pub.10.1038/nbt0502-484 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009100839
301 https://doi.org/10.1038/nbt0502-484
302 rdf:type schema:CreativeWork
303 sg:pub.10.1038/nbt0991-835 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034255207
304 https://doi.org/10.1038/nbt0991-835
305 rdf:type schema:CreativeWork
306 grid-institutes:None schema:alternateName Pien Gen Biomedical Corporation, Knoxville, TN USA
307 schema:name Pien Gen Biomedical Corporation, Knoxville, TN USA
308 rdf:type schema:Organization
309 grid-institutes:grid.410688.3 schema:alternateName Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland
310 schema:name Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland
311 rdf:type schema:Organization
312 grid-institutes:grid.419741.e schema:alternateName Department of Animal Reproduction, National Research Institute of Animal Production, 32-083 Balice, Poland
313 schema:name Department of Animal Reproduction, National Research Institute of Animal Production, 32-083 Balice, Poland
314 rdf:type schema:Organization
315 grid-institutes:grid.420230.7 schema:alternateName Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland
316 schema:name Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland
317 Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland
318 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...