Expression of human growth hormone in the milk of transgenic rabbits with transgene mapped to the telomere region of chromosome ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012-08-17

AUTHORS

Daniel Lipinski, Joanna Zeyland, Marlena Szalata, Andrzej Plawski, Malgorzata Jarmuz, Jacek Jura, Aleksandra Korcz, Zdzislaw Smorag, Marek Pienkowski, Ryszard Slomski

ABSTRACT

The advent of transgenic technology has provided methods for the production of pharmaceuticals by the isolation of these proteins from transgenic animals. The mammary gland has been focused on as a bioreactor, since milk is easily collected from lactating animals and protein production can be expressed at very high levels, including hormones and enzymes. We demonstrate here the expression pattern of recombinant human growth hormone (rhGH) in transgenic rabbits carrying hGH genomic sequences driven by the rat whey acidic protein (WAP) promoter. The transgene was mapped to the q26-27 telomere region of chromosome 7q by fluorescence in situ hybridization (FISH). Nearly 30 % of the F1 generation demonstrated the presence of transgene. The recombinant growth hormone was detected in the milk of the transgenic rabbit females, but not in serum, up to the level of 10 μg/ml. Ectopic expression of the transgene in the brain, heart, kidney, liver, and salivary gland was not observed, indicating that a short sequence of rat WAP promoter (969 bp) contained essential sequences directing expression exclusively to the mammary gland. The biological activity of recombinant growth hormone was measured by immunoreactivity and the capability to stimulate growth of the hormone-dependent Nb211 cell line. More... »

PAGES

435-442

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13353-012-0110-4

DOI

http://dx.doi.org/10.1007/s13353-012-0110-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000428364

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22898896


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66 hGH genomic sequences
67 heart
68 high levels
69 hormone
70 hormone-dependent Nb211 cell line
71 human growth hormone
72 hybridization
73 immunoreactivity
74 isolation
75 kidney
76 levels
77 lines
78 liver
79 mammary gland
80 method
81 milk
82 patterns
83 pharmaceuticals
84 presence
85 presence of transgenes
86 production
87 production of pharmaceuticals
88 promoter
89 protein
90 protein production
91 protein promoter
92 q26-27 telomere region
93 rabbit females
94 rabbits
95 rat WAP promoter
96 rats
97 recombinant growth hormone
98 recombinant human growth hormone
99 region
100 salivary glands
101 sequence
102 serum
103 short sequences
104 situ hybridization
105 technology
106 telomere regions
107 transgene
108 transgenic animals
109 transgenic rabbit females
110 transgenic rabbits
111 transgenic technology
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