PIK3CD promoted proliferation in diffuse large B cell lymphoma through upregulation of c-myc View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-07-22

AUTHORS

Wenli Cui, Shutao Zheng, Xinxia Li, Yuqing Ma, Wei Sang, Ming Liu, Wei Zhang, Xiaoyan Zhou

ABSTRACT

Despite PIK3CD has been extensively reported in cancers, however, little evidence has been available regarding its role in the setting of diffuse large B cell lymphoma (DLBCL). In the present study, to investigate the role of PIK3CD in DLBCL, relevant experiments were carried out on both in vivo clinical tissue level and in vitro cell line level. Prognostic and clinicopathological significance were analyzed after immunohistochemical assay of PIK3CD expression on DLBCL tissue microarray. MTT assay and flow cytometry were employed to evaluate the proliferative variation, cell cycle, and apoptosis. Athymic nude mice xenografted with DLBCL cell line were employed to confirm the role of PIK3CD. It was found that there was a significant difference between expression of PIK3CD and international prognosis index (IPI), performance state (PS), and inferior overall prognosis. Furthermore, PIK3CD can promote proliferation and prevent apoptosis in DLBCL cells in vitro through upregulation of c-myc and p-AKT and in contrast downregulation of p21 and p27. In nude mice model, knock-down of PIK3CD was shown to be able to suppress the proliferation of DLBCL but not significantly compared with control group. Taken together, our study showed that PIK3CD can promote proliferation of DLBCL cells both in vitro and in vivo, suggesting that PIK3CD could be druggable in the therapy of DLBCL. More... »

PAGES

12767-12777

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13277-016-5225-5

DOI

http://dx.doi.org/10.1007/s13277-016-5225-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041524107

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27448819


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38 schema:description Despite PIK3CD has been extensively reported in cancers, however, little evidence has been available regarding its role in the setting of diffuse large B cell lymphoma (DLBCL). In the present study, to investigate the role of PIK3CD in DLBCL, relevant experiments were carried out on both in vivo clinical tissue level and in vitro cell line level. Prognostic and clinicopathological significance were analyzed after immunohistochemical assay of PIK3CD expression on DLBCL tissue microarray. MTT assay and flow cytometry were employed to evaluate the proliferative variation, cell cycle, and apoptosis. Athymic nude mice xenografted with DLBCL cell line were employed to confirm the role of PIK3CD. It was found that there was a significant difference between expression of PIK3CD and international prognosis index (IPI), performance state (PS), and inferior overall prognosis. Furthermore, PIK3CD can promote proliferation and prevent apoptosis in DLBCL cells in vitro through upregulation of c-myc and p-AKT and in contrast downregulation of p21 and p27. In nude mice model, knock-down of PIK3CD was shown to be able to suppress the proliferation of DLBCL but not significantly compared with control group. Taken together, our study showed that PIK3CD can promote proliferation of DLBCL cells both in vitro and in vivo, suggesting that PIK3CD could be druggable in the therapy of DLBCL.
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46 DLBCL
47 DLBCL cell lines
48 DLBCL cells
49 DLBCL tissue microarray
50 MTT assay
51 Myc
52 PIK3CD
53 PIK3CD expression
54 apoptosis
55 assays
56 athymic nude mice
57 cancer
58 cell cycle
59 cell line level
60 cell lines
61 cell lymphoma
62 cells
63 clinical tissue level
64 clinicopathological significance
65 contrast downregulation
66 control group
67 cycle
68 cytometry
69 differences
70 diffuse
71 downregulation
72 evidence
73 experiments
74 expression
75 group
76 index
77 inferior overall prognosis
78 international prognosis index
79 levels
80 line level
81 lines
82 little evidence
83 lymphoma
84 mice
85 microarrays
86 model
87 mouse model
88 nude mice
89 nude mouse model
90 overall prognosis
91 p21
92 p27
93 performance state
94 present study
95 prognosis
96 prognosis index
97 proliferation
98 proliferation of DLBCL
99 proliferative variation
100 relevant experiments
101 role
102 role of PIK3CD
103 setting
104 setting of diffuse
105 significance
106 significant differences
107 state
108 study
109 therapy
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111 tissue levels
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113 upregulation
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