PIK3CD promoted proliferation in diffuse large B cell lymphoma through upregulation of c-myc View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-07-22

AUTHORS

Wenli Cui, Shutao Zheng, Xinxia Li, Yuqing Ma, Wei Sang, Ming Liu, Wei Zhang, Xiaoyan Zhou

ABSTRACT

Despite PIK3CD has been extensively reported in cancers, however, little evidence has been available regarding its role in the setting of diffuse large B cell lymphoma (DLBCL). In the present study, to investigate the role of PIK3CD in DLBCL, relevant experiments were carried out on both in vivo clinical tissue level and in vitro cell line level. Prognostic and clinicopathological significance were analyzed after immunohistochemical assay of PIK3CD expression on DLBCL tissue microarray. MTT assay and flow cytometry were employed to evaluate the proliferative variation, cell cycle, and apoptosis. Athymic nude mice xenografted with DLBCL cell line were employed to confirm the role of PIK3CD. It was found that there was a significant difference between expression of PIK3CD and international prognosis index (IPI), performance state (PS), and inferior overall prognosis. Furthermore, PIK3CD can promote proliferation and prevent apoptosis in DLBCL cells in vitro through upregulation of c-myc and p-AKT and in contrast downregulation of p21 and p27. In nude mice model, knock-down of PIK3CD was shown to be able to suppress the proliferation of DLBCL but not significantly compared with control group. Taken together, our study showed that PIK3CD can promote proliferation of DLBCL cells both in vitro and in vivo, suggesting that PIK3CD could be druggable in the therapy of DLBCL. More... »

PAGES

12767-12777

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13277-016-5225-5

DOI

http://dx.doi.org/10.1007/s13277-016-5225-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041524107

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27448819


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38 schema:description Despite PIK3CD has been extensively reported in cancers, however, little evidence has been available regarding its role in the setting of diffuse large B cell lymphoma (DLBCL). In the present study, to investigate the role of PIK3CD in DLBCL, relevant experiments were carried out on both in vivo clinical tissue level and in vitro cell line level. Prognostic and clinicopathological significance were analyzed after immunohistochemical assay of PIK3CD expression on DLBCL tissue microarray. MTT assay and flow cytometry were employed to evaluate the proliferative variation, cell cycle, and apoptosis. Athymic nude mice xenografted with DLBCL cell line were employed to confirm the role of PIK3CD. It was found that there was a significant difference between expression of PIK3CD and international prognosis index (IPI), performance state (PS), and inferior overall prognosis. Furthermore, PIK3CD can promote proliferation and prevent apoptosis in DLBCL cells in vitro through upregulation of c-myc and p-AKT and in contrast downregulation of p21 and p27. In nude mice model, knock-down of PIK3CD was shown to be able to suppress the proliferation of DLBCL but not significantly compared with control group. Taken together, our study showed that PIK3CD can promote proliferation of DLBCL cells both in vitro and in vivo, suggesting that PIK3CD could be druggable in the therapy of DLBCL.
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51 apoptosis
52 assays
53 athymic nude mice
54 c-Myc
55 cancer
56 cell cycle
57 cell line level
58 cell lines
59 cell lymphoma
60 cells
61 clinicopathological significance
62 control group
63 cycle
64 cytometry
65 differences
66 diffuse large B-cell lymphoma
67 downregulation
68 evidence
69 experiments
70 expression
71 group
72 index
73 inferior overall prognosis
74 international prognosis index
75 large B-cell lymphoma
76 levels
77 line level
78 lines
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80 lymphoma
81 mice
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85 nude mice
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87 overall prognosis
88 p-Akt
89 p21
90 p27
91 performance state
92 present study
93 prognosis
94 prognosis index
95 proliferation
96 relevant experiments
97 role
98 setting
99 significance
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101 state
102 study
103 therapy
104 tissue levels
105 tissue microarray
106 upregulation
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