Analysis of Helicobacter pylori genotypes in clinical gastric wash samples View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-01-29

AUTHORS

Shuichi Miyamoto, Yoshiyuki Watanabe, Ritsuko Oikawa, Shoko Ono, Katsuhiro Mabe, Takahiko Kudo, Hiroyuki Yamamoto, Fumio Itoh, Mototsugu Kato, Naoya Sakamoto

ABSTRACT

Helicobacter pylori is a key factor in the development of gastric cancer; indeed, clearance of H. pylori helps prevent gastric cancer. However, the relationship between gastric cancer and the abundance and diversity of H. pylori genotypes in the stomach remains unknown. Here, we present, for the first time, a quantitative analysis of H. pylori genotypes in gastric washes. A method was first developed to assess diversity and abundance by pyrosequencing and analysis of single nucleotide polymorphisms in 23S ribosomal RNA (rRNA), a gene associated with clarithromycin resistance. This method was then validated using arbitrarily mixed plasmids carrying 23S rRNA with single nucleotide polymorphisms. Multiple strains were detected in many of 34 clinical samples, with frequency 24.3 ± 24.2 and 26.3 ± 33.8 % for the A2143G and A2144G strains, respectively. Importantly, results obtained from gastric washes were similar to those obtained from biopsy samples. The method provides opportunities to investigate drug resistance in H. pylori and assess potential biomarkers of gastric cancer risk, and should thus be validated in large-scale clinical trials. More... »

PAGES

10123-10132

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13277-016-4886-4

DOI

http://dx.doi.org/10.1007/s13277-016-4886-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022784722

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26825980


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37 schema:description Helicobacter pylori is a key factor in the development of gastric cancer; indeed, clearance of H. pylori helps prevent gastric cancer. However, the relationship between gastric cancer and the abundance and diversity of H. pylori genotypes in the stomach remains unknown. Here, we present, for the first time, a quantitative analysis of H. pylori genotypes in gastric washes. A method was first developed to assess diversity and abundance by pyrosequencing and analysis of single nucleotide polymorphisms in 23S ribosomal RNA (rRNA), a gene associated with clarithromycin resistance. This method was then validated using arbitrarily mixed plasmids carrying 23S rRNA with single nucleotide polymorphisms. Multiple strains were detected in many of 34 clinical samples, with frequency 24.3 ± 24.2 and 26.3 ± 33.8 % for the A2143G and A2144G strains, respectively. Importantly, results obtained from gastric washes were similar to those obtained from biopsy samples. The method provides opportunities to investigate drug resistance in H. pylori and assess potential biomarkers of gastric cancer risk, and should thus be validated in large-scale clinical trials.
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45 H. pylori
46 H. pylori genotypes
47 Helicobacter pylori
48 Helicobacter pylori genotypes
49 RNA
50 abundance
51 analysis
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53 biopsy samples
54 cancer
55 cancer risk
56 clarithromycin resistance
57 clearance
58 clinical samples
59 clinical trials
60 development
61 diversity
62 drug resistance
63 factors
64 first time
65 gastric cancer
66 gastric cancer risk
67 gastric washes
68 genes
69 genotypes
70 key factors
71 large-scale clinical trials
72 method
73 mixed plasmids
74 multiple strains
75 nucleotide polymorphisms
76 opportunities
77 plasmid
78 polymorphism
79 potential biomarkers
80 pylori
81 pyrosequencing
82 quantitative analysis
83 relationship
84 resistance
85 results
86 ribosomal RNA
87 risk
88 samples
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