The role of miR-145 in stem cell characteristics of human laryngeal squamous cell carcinoma Hep-2 cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-10-22

AUTHORS

Omer Faruk Karatas, Ilknur Suer, Betul Yuceturk, Mehmet Yilmaz, Yusif Hajiyev, Chad J. Creighton, Michael Ittmann, Mustafa Ozen

ABSTRACT

The cancer stem-like cells (CSLCs) are tumorigenic cells promoting initiation, progression, and spread of the tumor. Accumulating evidences suggested the presence of CSLCs in distinct tumors including laryngeal squamous cell carcinoma (LSCC). MicroRNAs have been proposed as significant regulators of carcinogenesis, and several of them have been demonstrated to have direct roles in survival of CSLCs. In this study, we aimed to explore the role of miR-145, which is downregulated in LSCC, on cancer stem cell potency of laryngeal cancer cells. We initially showed the downregulation of miR-145 expression in tumor tissue samples and in CD133-enriched CSLCs. Quantitative reverse-transcription PCR (qRT-PCR) analysis of miR-145-transfected Hep-2 cells demonstrated the inhibitory role of miR-145 on stem cell markers like SOX2, OCT4, KLF4, and ABCG2. We, then, investigated the stem cell features of miR-145-overexpressing Hep-2 cells by sphere formation assay, single-cell cloning assay, and aldehyde dehydrogenase (ALDH) assay, which all demonstrated the inhibition of stem cell potency upon miR-145 overexpression. Further qRT-PCR analysis demonstrated altered expression of epithelial to mesenchymal transition markers in miR-145-overexpressing Hep-2 cells. In conclusion, we demonstrated the regulatory role of miR-145 in stem cell characteristics of Hep-2 cells. Based on these results, we propose that miR-145 might carry crucial roles in LSCC tumorigenesis, prognosis, metastasis, chemoresistance, and recurrence through regulating stem cell properties of tumor cells. More... »

PAGES

4183-4192

References to SciGraph publications

  • 2003-09-28. Vertebrate neurogenesis is counteracted by Sox1–3 activity in NATURE NEUROSCIENCE
  • 2006-12-19. Beyond tumorigenesis: cancer stem cells in metastasis in CELL RESEARCH
  • 2009-01-24. EMT, the cytoskeleton, and cancer cell invasion in CANCER AND METASTASIS REVIEWS
  • 2007-01. Signaling Pathways in Cancer and Embryonic Stem Cells in STEM CELL REVIEWS AND REPORTS
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    http://scigraph.springernature.com/pub.10.1007/s13277-015-4219-z

    DOI

    http://dx.doi.org/10.1007/s13277-015-4219-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1006034022

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26490990


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