Exosomes from docetaxel-resistant breast cancer cells alter chemosensitivity by delivering microRNAs View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-10

AUTHORS

Wei-xian Chen, Yan-qin Cai, Meng-meng Lv, Lin Chen, Shan-liang Zhong, Teng-fei Ma, Jian-hua Zhao, Jin-hai Tang

ABSTRACT

Breast cancer (BCa) remains chemo-unresponsive by inevitable progression of resistance to first-line treatment with docetaxel (doc). Emerging studies indicate that exosomes act as mediators of intercellular communication between heterogeneous populations of tumor cells, engendering a transmitted drug resistance for cancer development. Such modulatory effects have been related to the constant shuttle of biologically active molecules including microRNAs (miRNAs). Here, we aimed to investigate the relevance of exosome-mediated miRNA delivery in resistance transmission of BCa subpopulations. Using microarray and polymerase chain reaction, we found that exosomes from doc-resistant BCa cells (D/exo) loaded cellular miRNAs. Following D/exo transfer to the fluorescent sensitive cells (GFP-S), some miRNAs were significantly increased in recipient GFP-S. Target gene prediction and pathway analysis revealed the involvement of the top 20 most abundant miRNAs of D/exo in pathways implicated in therapy failure. Coculture assays showed that miRNA-containing D/exo increased the overall resistance of GFP-S to doc exposure. Moreover, D/exo was able to alter gene expression in GFP-S. Our results open up an intriguing possibility that drug-resistant BCa cells may spread chemoresistance to sensitive ones by releasing exosomes and that the effects could be partly attributed to the intercellular transfer of specific miRNAs. More... »

PAGES

9649-9659

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13277-014-2242-0

DOI

http://dx.doi.org/10.1007/s13277-014-2242-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029113516

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24969560


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s13277-014-2242-0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s13277-014-2242-0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s13277-014-2242-0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s13277-014-2242-0'


 

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302 schema:name Department of Thoracic Surgery, Nanjing Medical University Affiliated Huai’an First People’s Hospital, Huai’an, China
303 rdf:type schema:Organization
 




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