Andrographolide induce human embryonic stem cell apoptosis by oxidative stress response View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-03-29

AUTHORS

Huarong Huang, Huanhuan Cao, Chun Xing, Yunfen Hua, Ming Zhang, Lifang Jin

ABSTRACT

BackgroundsThe anti-inflammatory effect of andrographolide is widely accepted; however, its exact role in reproductive toxicity requires further elucidation. The embryonic stem cell test (EST) is a promising system for detecting the reproductive toxicity of drugs in vitro. In this study, we applied a prediction model to our EST data after classifying andrographolide according to published criteria. The possible mechanism of andrographolide reproductive toxicity was also studied.MethodsReproductive toxicity of andrographolide was evaluated in vitro EST model and in vivo mouse model. Human embryonic stem cells (ESCs) were cultured with different concentrations of andrographolide with or without N-acetyl-L-cysteine (NAC). Cell viability was assessed with MTT assay, and reactive oxygen species (ROS) level was measured with DCFH-DA assay. Gene and protein expression levels were measured with qRT-PCR and western-blot, respectively.ResultsResults showed that andrographolide exhibited strong reproductive toxicity according to the prediction model of the EST and mouse studies. An increase in ROS levels, damage to mitochondrial membrane potential, and induction of caspase-3 were observed in the andrographolide-treated human ESCs. Scavenging of andrographolide-induced ROS by NAC blocked these activities. Western blot and qRT-PCR analysis revealed that the nuclear factor erythroid-2-related factor 2 (Nrf2) protein and its target antioxidant genes were up-regulated after andrographolide treatment at certain concentrations. Furthermore, NAC treatment significantly increased the activity of the Nrf2 signaling pathway.ConclusionWe demonstrated that andrographolide is a drug with strong reproductive toxicity, which resulted from ROS-mediated oxidative stress. In addition, the Nrf2 pathway appears to be involved in the NAC protection of human ESCs against andrographolide-induced cell apoptosis. More... »

PAGES

209-219

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13273-019-0024-x

DOI

http://dx.doi.org/10.1007/s13273-019-0024-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1113116051


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Hangzhou Precision Medicine Research Center, Hangzhou, China", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, China", 
            "Hangzhou Precision Medicine Research Center, Hangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huang", 
        "givenName": "Huarong", 
        "id": "sg:person.011062006441.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011062006441.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hangzhou Economic and Technology Development Area Biomedical Public Platform, Hangzhou, China", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Hangzhou Precision Medicine Research Center, Hangzhou, China", 
            "Hangzhou Economic and Technology Development Area Biomedical Public Platform, Hangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cao", 
        "givenName": "Huanhuan", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, China", 
          "id": "http://www.grid.ac/institutes/grid.410595.c", 
          "name": [
            "College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xing", 
        "givenName": "Chun", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "College of pharmacy pharmaceutical science, Zhejiang University of Technology, Hangzhou, China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Hangzhou Precision Medicine Research Center, Hangzhou, China", 
            "College of pharmacy pharmaceutical science, Zhejiang University of Technology, Hangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hua", 
        "givenName": "Yunfen", 
        "id": "sg:person.0775225771.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0775225771.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "College of Life Science, Zhejiang University, Hangzhou, China", 
          "id": "http://www.grid.ac/institutes/grid.13402.34", 
          "name": [
            "Hangzhou Precision Medicine Research Center, Hangzhou, China", 
            "College of Life Science, Zhejiang University, Hangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Ming", 
        "id": "sg:person.015442651441.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015442651441.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hangzhou Precision Medicine Research Center, Hangzhou, China", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "College of Life Science, Shaoxing University, Shaoxing, Zhejiang, China", 
            "Hangzhou Precision Medicine Research Center, Hangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jin", 
        "givenName": "Lifang", 
        "id": "sg:person.01210022734.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210022734.76"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/sj.onc.1207534", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041233854", 
          "https://doi.org/10.1038/sj.onc.1207534"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4401231", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040097797", 
          "https://doi.org/10.1038/sj.cdd.4401231"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cr.2010.178", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003912984", 
          "https://doi.org/10.1038/cr.2010.178"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2011.348", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042547608", 
          "https://doi.org/10.1038/nprot.2011.348"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12929-016-0257-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007269938", 
          "https://doi.org/10.1186/s12929-016-0257-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cr.2016.59", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033727244", 
          "https://doi.org/10.1038/cr.2016.59"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-03-29", 
    "datePublishedReg": "2019-03-29", 
    "description": "BackgroundsThe anti-inflammatory effect of andrographolide is widely accepted; however, its exact role in reproductive toxicity requires further elucidation. The embryonic stem cell test (EST) is a promising system for detecting the reproductive toxicity of drugs in vitro. In this study, we applied a prediction model to our EST data after classifying andrographolide according to published criteria. The possible mechanism of andrographolide reproductive toxicity was also studied.MethodsReproductive toxicity of andrographolide was evaluated in vitro EST model and in vivo mouse model. Human embryonic stem cells (ESCs) were cultured with different concentrations of andrographolide with or without N-acetyl-L-cysteine (NAC). Cell viability was assessed with MTT assay, and reactive oxygen species (ROS) level was measured with DCFH-DA assay. Gene and protein expression levels were measured with qRT-PCR and western-blot, respectively.ResultsResults showed that andrographolide exhibited strong reproductive toxicity according to the prediction model of the EST and mouse studies. An increase in ROS levels, damage to mitochondrial membrane potential, and induction of caspase-3 were observed in the andrographolide-treated human ESCs. Scavenging of andrographolide-induced ROS by NAC blocked these activities. Western blot and qRT-PCR analysis revealed that the nuclear factor erythroid-2-related factor 2 (Nrf2) protein and its target antioxidant genes were up-regulated after andrographolide treatment at certain concentrations. Furthermore, NAC treatment significantly increased the activity of the Nrf2 signaling pathway.ConclusionWe demonstrated that andrographolide is a drug with strong reproductive toxicity, which resulted from ROS-mediated oxidative stress. In addition, the Nrf2 pathway appears to be involved in the NAC protection of human ESCs against andrographolide-induced cell apoptosis.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s13273-019-0024-x", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1040680", 
        "issn": [
          "1738-642X", 
          "2092-8467"
        ], 
        "name": "Molecular & Cellular Toxicology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "keywords": [
      "human embryonic stem cells", 
      "embryonic stem cells", 
      "embryonic stem cell test", 
      "strong reproductive toxicity", 
      "oxidative stress response", 
      "cell apoptosis", 
      "qRT-PCR analysis", 
      "mitochondrial membrane potential", 
      "factor 2 (Nrf2) protein", 
      "stem cell apoptosis", 
      "EST data", 
      "reproductive toxicity", 
      "oxygen species levels", 
      "species level", 
      "target antioxidant genes", 
      "protein expression levels", 
      "stress response", 
      "antioxidant genes", 
      "DCFH-DA assay", 
      "stem cells", 
      "qRT-PCR", 
      "ROS levels", 
      "caspase-3", 
      "expression levels", 
      "nuclear factor", 
      "membrane potential", 
      "genes", 
      "vivo mouse model", 
      "cell viability", 
      "oxidative stress", 
      "Western blot", 
      "Nrf2 pathway", 
      "further elucidation", 
      "apoptosis", 
      "NAC protection", 
      "pathway", 
      "MTT assay", 
      "ROS", 
      "exact role", 
      "mouse studies", 
      "andrographolide treatment", 
      "assays", 
      "mouse model", 
      "protein", 
      "EST model", 
      "cysteine", 
      "possible mechanism", 
      "toxicity", 
      "Nrf2", 
      "elucidation", 
      "blot", 
      "activity", 
      "cells", 
      "induction", 
      "anti-inflammatory effects", 
      "andrographolide", 
      "different concentrations", 
      "viability", 
      "levels", 
      "NAC treatment", 
      "certain concentration", 
      "acetyl", 
      "NAC", 
      "promising system", 
      "mechanism", 
      "ConclusionWe", 
      "role", 
      "stress", 
      "response", 
      "damage", 
      "concentration", 
      "cell test", 
      "drugs", 
      "study", 
      "treatment", 
      "potential", 
      "factors", 
      "addition", 
      "protection", 
      "analysis", 
      "increase", 
      "effect", 
      "ResultsResults", 
      "model", 
      "data", 
      "system", 
      "prediction model", 
      "criteria", 
      "test", 
      "stem cell test", 
      "andrographolide reproductive toxicity", 
      "MethodsReproductive toxicity", 
      "andrographolide-treated human ESCs", 
      "andrographolide-induced ROS", 
      "andrographolide-induced cell apoptosis", 
      "human embryonic stem cell apoptosis", 
      "embryonic stem cell apoptosis"
    ], 
    "name": "Andrographolide induce human embryonic stem cell apoptosis by oxidative stress response", 
    "pagination": "209-219", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1113116051"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s13273-019-0024-x"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s13273-019-0024-x", 
      "https://app.dimensions.ai/details/publication/pub.1113116051"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:45", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_815.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s13273-019-0024-x"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s13273-019-0024-x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s13273-019-0024-x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s13273-019-0024-x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s13273-019-0024-x'


 

This table displays all metadata directly associated to this object as RDF triples.

227 TRIPLES      22 PREDICATES      128 URIs      114 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s13273-019-0024-x schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N32aee4e8c14247a48dae2226197b16f0
4 schema:citation sg:pub.10.1038/cr.2010.178
5 sg:pub.10.1038/cr.2016.59
6 sg:pub.10.1038/nprot.2011.348
7 sg:pub.10.1038/sj.cdd.4401231
8 sg:pub.10.1038/sj.onc.1207534
9 sg:pub.10.1186/s12929-016-0257-0
10 schema:datePublished 2019-03-29
11 schema:datePublishedReg 2019-03-29
12 schema:description BackgroundsThe anti-inflammatory effect of andrographolide is widely accepted; however, its exact role in reproductive toxicity requires further elucidation. The embryonic stem cell test (EST) is a promising system for detecting the reproductive toxicity of drugs in vitro. In this study, we applied a prediction model to our EST data after classifying andrographolide according to published criteria. The possible mechanism of andrographolide reproductive toxicity was also studied.MethodsReproductive toxicity of andrographolide was evaluated in vitro EST model and in vivo mouse model. Human embryonic stem cells (ESCs) were cultured with different concentrations of andrographolide with or without N-acetyl-L-cysteine (NAC). Cell viability was assessed with MTT assay, and reactive oxygen species (ROS) level was measured with DCFH-DA assay. Gene and protein expression levels were measured with qRT-PCR and western-blot, respectively.ResultsResults showed that andrographolide exhibited strong reproductive toxicity according to the prediction model of the EST and mouse studies. An increase in ROS levels, damage to mitochondrial membrane potential, and induction of caspase-3 were observed in the andrographolide-treated human ESCs. Scavenging of andrographolide-induced ROS by NAC blocked these activities. Western blot and qRT-PCR analysis revealed that the nuclear factor erythroid-2-related factor 2 (Nrf2) protein and its target antioxidant genes were up-regulated after andrographolide treatment at certain concentrations. Furthermore, NAC treatment significantly increased the activity of the Nrf2 signaling pathway.ConclusionWe demonstrated that andrographolide is a drug with strong reproductive toxicity, which resulted from ROS-mediated oxidative stress. In addition, the Nrf2 pathway appears to be involved in the NAC protection of human ESCs against andrographolide-induced cell apoptosis.
13 schema:genre article
14 schema:inLanguage en
15 schema:isAccessibleForFree false
16 schema:isPartOf N0979c033d8044682967ae7f7739b392f
17 Na875fdb7d7774fc8984670f0a46e7590
18 sg:journal.1040680
19 schema:keywords ConclusionWe
20 DCFH-DA assay
21 EST data
22 EST model
23 MTT assay
24 MethodsReproductive toxicity
25 NAC
26 NAC protection
27 NAC treatment
28 Nrf2
29 Nrf2 pathway
30 ROS
31 ROS levels
32 ResultsResults
33 Western blot
34 acetyl
35 activity
36 addition
37 analysis
38 andrographolide
39 andrographolide reproductive toxicity
40 andrographolide treatment
41 andrographolide-induced ROS
42 andrographolide-induced cell apoptosis
43 andrographolide-treated human ESCs
44 anti-inflammatory effects
45 antioxidant genes
46 apoptosis
47 assays
48 blot
49 caspase-3
50 cell apoptosis
51 cell test
52 cell viability
53 cells
54 certain concentration
55 concentration
56 criteria
57 cysteine
58 damage
59 data
60 different concentrations
61 drugs
62 effect
63 elucidation
64 embryonic stem cell apoptosis
65 embryonic stem cell test
66 embryonic stem cells
67 exact role
68 expression levels
69 factor 2 (Nrf2) protein
70 factors
71 further elucidation
72 genes
73 human embryonic stem cell apoptosis
74 human embryonic stem cells
75 increase
76 induction
77 levels
78 mechanism
79 membrane potential
80 mitochondrial membrane potential
81 model
82 mouse model
83 mouse studies
84 nuclear factor
85 oxidative stress
86 oxidative stress response
87 oxygen species levels
88 pathway
89 possible mechanism
90 potential
91 prediction model
92 promising system
93 protection
94 protein
95 protein expression levels
96 qRT-PCR
97 qRT-PCR analysis
98 reproductive toxicity
99 response
100 role
101 species level
102 stem cell apoptosis
103 stem cell test
104 stem cells
105 stress
106 stress response
107 strong reproductive toxicity
108 study
109 system
110 target antioxidant genes
111 test
112 toxicity
113 treatment
114 viability
115 vivo mouse model
116 schema:name Andrographolide induce human embryonic stem cell apoptosis by oxidative stress response
117 schema:pagination 209-219
118 schema:productId N1f8cf56c042c41e3bb3c561c36ddbb0f
119 Nedbf93c8b1254dca9f7bb6298956b75d
120 schema:sameAs https://app.dimensions.ai/details/publication/pub.1113116051
121 https://doi.org/10.1007/s13273-019-0024-x
122 schema:sdDatePublished 2021-12-01T19:45
123 schema:sdLicense https://scigraph.springernature.com/explorer/license/
124 schema:sdPublisher N323a6a3e36b64d0abab2c8e3c63ea4ce
125 schema:url https://doi.org/10.1007/s13273-019-0024-x
126 sgo:license sg:explorer/license/
127 sgo:sdDataset articles
128 rdf:type schema:ScholarlyArticle
129 N0979c033d8044682967ae7f7739b392f schema:volumeNumber 15
130 rdf:type schema:PublicationVolume
131 N0cb0aa33250c426fbb06acaab741c2be schema:affiliation grid-institutes:None
132 schema:familyName Cao
133 schema:givenName Huanhuan
134 rdf:type schema:Person
135 N1f8cf56c042c41e3bb3c561c36ddbb0f schema:name dimensions_id
136 schema:value pub.1113116051
137 rdf:type schema:PropertyValue
138 N20e9d1f4e18c426aabf4fbfeb80f8edc rdf:first sg:person.015442651441.49
139 rdf:rest N542a58216a54452ba9f6c98977f8ebe4
140 N323a6a3e36b64d0abab2c8e3c63ea4ce schema:name Springer Nature - SN SciGraph project
141 rdf:type schema:Organization
142 N32aee4e8c14247a48dae2226197b16f0 rdf:first sg:person.011062006441.35
143 rdf:rest Nc0ef7e3dfe654594a3c4fd0cac2edf8b
144 N3d9a124b4c554fa58a0f61a9d5ee0a10 rdf:first Nea3e7133963542c880b3f04667849c44
145 rdf:rest Nbdde23c9afe0486b9d2e3d63171c1c55
146 N542a58216a54452ba9f6c98977f8ebe4 rdf:first sg:person.01210022734.76
147 rdf:rest rdf:nil
148 Na875fdb7d7774fc8984670f0a46e7590 schema:issueNumber 2
149 rdf:type schema:PublicationIssue
150 Nbdde23c9afe0486b9d2e3d63171c1c55 rdf:first sg:person.0775225771.61
151 rdf:rest N20e9d1f4e18c426aabf4fbfeb80f8edc
152 Nc0ef7e3dfe654594a3c4fd0cac2edf8b rdf:first N0cb0aa33250c426fbb06acaab741c2be
153 rdf:rest N3d9a124b4c554fa58a0f61a9d5ee0a10
154 Nea3e7133963542c880b3f04667849c44 schema:affiliation grid-institutes:grid.410595.c
155 schema:familyName Xing
156 schema:givenName Chun
157 rdf:type schema:Person
158 Nedbf93c8b1254dca9f7bb6298956b75d schema:name doi
159 schema:value 10.1007/s13273-019-0024-x
160 rdf:type schema:PropertyValue
161 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
162 schema:name Biological Sciences
163 rdf:type schema:DefinedTerm
164 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
165 schema:name Biochemistry and Cell Biology
166 rdf:type schema:DefinedTerm
167 sg:journal.1040680 schema:issn 1738-642X
168 2092-8467
169 schema:name Molecular & Cellular Toxicology
170 schema:publisher Springer Nature
171 rdf:type schema:Periodical
172 sg:person.011062006441.35 schema:affiliation grid-institutes:None
173 schema:familyName Huang
174 schema:givenName Huarong
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011062006441.35
176 rdf:type schema:Person
177 sg:person.01210022734.76 schema:affiliation grid-institutes:None
178 schema:familyName Jin
179 schema:givenName Lifang
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210022734.76
181 rdf:type schema:Person
182 sg:person.015442651441.49 schema:affiliation grid-institutes:grid.13402.34
183 schema:familyName Zhang
184 schema:givenName Ming
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015442651441.49
186 rdf:type schema:Person
187 sg:person.0775225771.61 schema:affiliation grid-institutes:grid.469325.f
188 schema:familyName Hua
189 schema:givenName Yunfen
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0775225771.61
191 rdf:type schema:Person
192 sg:pub.10.1038/cr.2010.178 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003912984
193 https://doi.org/10.1038/cr.2010.178
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/cr.2016.59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033727244
196 https://doi.org/10.1038/cr.2016.59
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/nprot.2011.348 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042547608
199 https://doi.org/10.1038/nprot.2011.348
200 rdf:type schema:CreativeWork
201 sg:pub.10.1038/sj.cdd.4401231 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040097797
202 https://doi.org/10.1038/sj.cdd.4401231
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/sj.onc.1207534 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041233854
205 https://doi.org/10.1038/sj.onc.1207534
206 rdf:type schema:CreativeWork
207 sg:pub.10.1186/s12929-016-0257-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007269938
208 https://doi.org/10.1186/s12929-016-0257-0
209 rdf:type schema:CreativeWork
210 grid-institutes:None schema:alternateName Hangzhou Economic and Technology Development Area Biomedical Public Platform, Hangzhou, China
211 Hangzhou Precision Medicine Research Center, Hangzhou, China
212 schema:name College of Life Science, Shaoxing University, Shaoxing, Zhejiang, China
213 College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, China
214 Hangzhou Economic and Technology Development Area Biomedical Public Platform, Hangzhou, China
215 Hangzhou Precision Medicine Research Center, Hangzhou, China
216 rdf:type schema:Organization
217 grid-institutes:grid.13402.34 schema:alternateName College of Life Science, Zhejiang University, Hangzhou, China
218 schema:name College of Life Science, Zhejiang University, Hangzhou, China
219 Hangzhou Precision Medicine Research Center, Hangzhou, China
220 rdf:type schema:Organization
221 grid-institutes:grid.410595.c schema:alternateName College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, China
222 schema:name College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, China
223 rdf:type schema:Organization
224 grid-institutes:grid.469325.f schema:alternateName College of pharmacy pharmaceutical science, Zhejiang University of Technology, Hangzhou, China
225 schema:name College of pharmacy pharmaceutical science, Zhejiang University of Technology, Hangzhou, China
226 Hangzhou Precision Medicine Research Center, Hangzhou, China
227 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...