Expression and characterization of a recombinant 2,3-dihydroxybiphenyl-1,2-dioxygenase from Pseudomonas View Full Text


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Article Info

DATE

2012-12

AUTHORS

Fei Xiong, Jian-Jun Shuai, Xiao-Fen Jin, Jian Zhang, Jing Sun, Ri-He Peng, Quan-Hong Yao, Ai-Sheng Xiong

ABSTRACT

The 2, 3-dihydroxybiphenyl-1,2-dioxygenase, which can degrade the 2,3-dihydroxybiphenyl, was encoded by a synthesized PsbphCI gene. The PsbphCI gene was transformed into Escherichia coli and the encoding protein purified, had a molecular mass of ∼32 kDa as determined by SDS-PAGE. The optimum pH for the purified enzyme at 20°C was 9.0, and the optimal temperature at pH 8.0 was 30°C. Subsequently, the PsbphCI gene was transformed into Pseudomonas putida sp. to verify the degradation of 2,3-dihydroxybiphenyl by HPLC. The transgenic EG11 strain degraded 65.20% of the 2,3-DHBP after 2 minutes at 30°C, while the wild-type EG11 strain degraded only 37.75%. This study provides guidance for the cultivation of bioengineered biphenyl/PCBs-degrading bacteria which can be applied to the biodegradation of environmental biphenyl/PCBs contamination. More... »

PAGES

375-382

References to SciGraph publications

  • 2006-10-05. A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2007-05-24. Biphenyl-utilizing bacteria and their functional genes in a pine root zone contaminated with polychlorinated biphenyls (PCBs) in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2007-05-30. Characterization of multiple novel aerobic polychlorinated biphenyl (PCB)-utilizing bacterial strains indigenous to contaminated tropical African soils in BIODEGRADATION
  • 2010-04-20. Identification and Analysis of Polychlorinated Biphenyls (PCBs)-Biodegrading Bacterial Strains in Shanghai in CURRENT MICROBIOLOGY
  • 2006-03-20. Intensification of the aerobic bioremediation of an actual site soil historically contaminated by polychlorinated biphenyls (PCBs) through bioaugmentation with a non acclimated, complex source of microorganisms in MICROBIAL CELL FACTORIES
  • 1994-12. Molecular genetics and evolutionary relationship of PCB-degrading bacteria in BIODEGRADATION
  • 2007-12. Bioremediation of soils and sediments contaminated by polychlorinated biphenyls in MICROBIOLOGY
  • 2004-07-10. Strategies for bioremediation of polychlorinated biphenyls in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2006-07-13. PCR-based accurate synthesis of long DNA sequences in NATURE PROTOCOLS
  • 2000-02. Role of the reactor configuration in the biological detoxification of a dump site-polychlorobiphenyl-contaminated soil in lab-scale slurry phase conditions in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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    http://scigraph.springernature.com/pub.10.1007/s13273-012-0046-0

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    http://dx.doi.org/10.1007/s13273-012-0046-0

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