Cyclooxygenase-2 inhibition by novel Bisaryl imidazolyl imidazole derivatives increases Bax/Bcl-2 ratio and upregulates Caspase-3 gene expression in Caco-2 colorectal cancer ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-04-16

AUTHORS

Reza Entezari Heravi, Farzin Hadizadeh, Mojtaba Sankian, Jalil Tavakol Afshari, Javad Behravan

ABSTRACT

Cyclooxygenase-2 (COX-2) inhibitors including celecoxib inhibit cell growth and induce apoptosis in cancer cells. In this study, the relation of Bax (an apoptosis promoter) to Bcl-2 (an apoptosis inhibitor) ratio with the apoptosis co-ordination enzyme, caspase-3 was investigated in correlation with the treatment of 4,5-bisaryl imidazolyl imidazoles as novel selective COX-2 inhibitors in Caco-2 colorectal cancer cells. Recently, the organic reactions under microwave irradiation attracted attention of scientists due to their high reaction rate, mild reaction conditions and the formation of clean products. Therefore, a microwave-assisted method was used to synthesize our compounds. The effects of these COX-2 inhibitors on the proliferation of Caco-2 cells were evaluated by MTT assay. cDNA microarray and clustering analysis were used to evaluate effects of our synthetic compounds on gene expression pattern of 112 genes involved in apoptosis pathways. Bax, Bcl-2 and caspase-3 mRNA expression and their relationship were analyzed by quantitative real-time PCR. Results indicated that proliferation of Caco-2 cells after treatment with 4,5-bisaryl imidazolyl imidazoles on Caco-2 cells were time and dose dependent. We conclude that increase in Bax/Bcl-2 ratio leads to an up-regulation in caspase-3 mRNA expression. More... »

PAGES

199-204

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13258-011-0168-0

DOI

http://dx.doi.org/10.1007/s13258-011-0168-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045853595


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Pharmaceutical Biotechnology, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran", 
            "Department of Pharmaceutical Biotechnology, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Heravi", 
        "givenName": "Reza Entezari", 
        "id": "sg:person.01153272413.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153272413.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medicinal Chemistry, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran", 
            "Department of Medicinal Chemistry, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hadizadeh", 
        "givenName": "Farzin", 
        "id": "sg:person.01342306074.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342306074.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sankian", 
        "givenName": "Mojtaba", 
        "id": "sg:person.01100253272.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100253272.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Afshari", 
        "givenName": "Jalil Tavakol", 
        "id": "sg:person.0677401417.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677401417.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pharmaceutical Biotechnology, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran", 
            "Department of Pharmaceutical Biotechnology, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Behravan", 
        "givenName": "Javad", 
        "id": "sg:person.01245756105.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245756105.18"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s00011-009-0034-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025968774", 
          "https://doi.org/10.1007/s00011-009-0034-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/348334a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005542034", 
          "https://doi.org/10.1038/348334a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1210113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025777644", 
          "https://doi.org/10.1038/sj.onc.1210113"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-04-16", 
    "datePublishedReg": "2012-04-16", 
    "description": "Cyclooxygenase-2 (COX-2) inhibitors including celecoxib inhibit cell growth and induce apoptosis in cancer cells. In this study, the relation of Bax (an apoptosis promoter) to Bcl-2 (an apoptosis inhibitor) ratio with the apoptosis co-ordination enzyme, caspase-3 was investigated in correlation with the treatment of 4,5-bisaryl imidazolyl imidazoles as novel selective COX-2 inhibitors in Caco-2 colorectal cancer cells. Recently, the organic reactions under microwave irradiation attracted attention of scientists due to their high reaction rate, mild reaction conditions and the formation of clean products. Therefore, a microwave-assisted method was used to synthesize our compounds. The effects of these COX-2 inhibitors on the proliferation of Caco-2 cells were evaluated by MTT assay. cDNA microarray and clustering analysis were used to evaluate effects of our synthetic compounds on gene expression pattern of 112 genes involved in apoptosis pathways. Bax, Bcl-2 and caspase-3 mRNA expression and their relationship were analyzed by quantitative real-time PCR. Results indicated that proliferation of Caco-2 cells after treatment with 4,5-bisaryl imidazolyl imidazoles on Caco-2 cells were time and dose dependent. We conclude that increase in Bax/Bcl-2 ratio leads to an up-regulation in caspase-3 mRNA expression.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s13258-011-0168-0", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1040407", 
        "issn": [
          "0254-5934", 
          "2092-9293"
        ], 
        "name": "Genes & Genomics", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "34"
      }
    ], 
    "keywords": [
      "Bax/Bcl-2 ratio", 
      "Bcl-2 ratio", 
      "Caco-2 colorectal cancer cells", 
      "gene expression patterns", 
      "Caco-2 cells", 
      "quantitative real-time PCR", 
      "cancer cells", 
      "caspase-3 mRNA expression", 
      "caspase-3 gene expression", 
      "cDNA microarray", 
      "Caco-2 colorectal cancer cell line", 
      "real-time PCR", 
      "expression patterns", 
      "gene expression", 
      "apoptosis pathway", 
      "colorectal cancer cells", 
      "cell growth", 
      "colorectal cancer cell lines", 
      "mRNA expression", 
      "cancer cell lines", 
      "cell lines", 
      "expression", 
      "cells", 
      "novel selective COX-2 inhibitor", 
      "attention of scientists", 
      "MTT assay", 
      "inhibitors", 
      "proliferation", 
      "synthetic compounds", 
      "genes", 
      "microarrays", 
      "enzyme", 
      "apoptosis", 
      "Bax", 
      "pathway", 
      "Bcl-2", 
      "caspase-3", 
      "PCR", 
      "assays", 
      "growth", 
      "inhibition", 
      "lines", 
      "compounds", 
      "patterns", 
      "formation", 
      "COX-2 inhibitors", 
      "effect", 
      "products", 
      "analysis", 
      "treatment", 
      "conditions", 
      "increase", 
      "cyclooxygenase-2 inhibitor", 
      "relationship", 
      "scientists", 
      "study", 
      "imidazole", 
      "selective COX-2 inhibitors", 
      "results", 
      "derivatives", 
      "ratio", 
      "rate", 
      "reaction", 
      "correlation", 
      "relation", 
      "time", 
      "higher reaction rate", 
      "cyclooxygenase-2 inhibition", 
      "celecoxib", 
      "imidazole derivatives", 
      "irradiation", 
      "attention", 
      "method", 
      "reaction rate", 
      "clean products", 
      "dose", 
      "reaction conditions", 
      "organic reactions", 
      "mild reaction conditions", 
      "microwave-assisted method", 
      "microwave irradiation", 
      "relation of Bax", 
      "apoptosis co-ordination enzyme", 
      "co-ordination enzyme", 
      "imidazolyl imidazoles", 
      "novel Bisaryl imidazolyl imidazole derivatives", 
      "Bisaryl imidazolyl imidazole derivatives", 
      "imidazolyl imidazole derivatives"
    ], 
    "name": "Cyclooxygenase-2 inhibition by novel Bisaryl imidazolyl imidazole derivatives increases Bax/Bcl-2 ratio and upregulates Caspase-3 gene expression in Caco-2 colorectal cancer cell line", 
    "pagination": "199-204", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1045853595"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s13258-011-0168-0"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s13258-011-0168-0", 
      "https://app.dimensions.ai/details/publication/pub.1045853595"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_562.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s13258-011-0168-0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s13258-011-0168-0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s13258-011-0168-0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s13258-011-0168-0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s13258-011-0168-0'


 

This table displays all metadata directly associated to this object as RDF triples.

195 TRIPLES      22 PREDICATES      117 URIs      105 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s13258-011-0168-0 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 anzsrc-for:0604
4 schema:author N38f25cd197934937bb425ca462e452f6
5 schema:citation sg:pub.10.1007/s00011-009-0034-6
6 sg:pub.10.1038/348334a0
7 sg:pub.10.1038/sj.onc.1210113
8 schema:datePublished 2012-04-16
9 schema:datePublishedReg 2012-04-16
10 schema:description Cyclooxygenase-2 (COX-2) inhibitors including celecoxib inhibit cell growth and induce apoptosis in cancer cells. In this study, the relation of Bax (an apoptosis promoter) to Bcl-2 (an apoptosis inhibitor) ratio with the apoptosis co-ordination enzyme, caspase-3 was investigated in correlation with the treatment of 4,5-bisaryl imidazolyl imidazoles as novel selective COX-2 inhibitors in Caco-2 colorectal cancer cells. Recently, the organic reactions under microwave irradiation attracted attention of scientists due to their high reaction rate, mild reaction conditions and the formation of clean products. Therefore, a microwave-assisted method was used to synthesize our compounds. The effects of these COX-2 inhibitors on the proliferation of Caco-2 cells were evaluated by MTT assay. cDNA microarray and clustering analysis were used to evaluate effects of our synthetic compounds on gene expression pattern of 112 genes involved in apoptosis pathways. Bax, Bcl-2 and caspase-3 mRNA expression and their relationship were analyzed by quantitative real-time PCR. Results indicated that proliferation of Caco-2 cells after treatment with 4,5-bisaryl imidazolyl imidazoles on Caco-2 cells were time and dose dependent. We conclude that increase in Bax/Bcl-2 ratio leads to an up-regulation in caspase-3 mRNA expression.
11 schema:genre article
12 schema:inLanguage en
13 schema:isAccessibleForFree false
14 schema:isPartOf N8e5e9ae45353415e915b59d0c7465722
15 Ne17fc76b63a843efaf5343b1d607eba2
16 sg:journal.1040407
17 schema:keywords Bax
18 Bax/Bcl-2 ratio
19 Bcl-2
20 Bcl-2 ratio
21 Bisaryl imidazolyl imidazole derivatives
22 COX-2 inhibitors
23 Caco-2 cells
24 Caco-2 colorectal cancer cell line
25 Caco-2 colorectal cancer cells
26 MTT assay
27 PCR
28 analysis
29 apoptosis
30 apoptosis co-ordination enzyme
31 apoptosis pathway
32 assays
33 attention
34 attention of scientists
35 cDNA microarray
36 cancer cell lines
37 cancer cells
38 caspase-3
39 caspase-3 gene expression
40 caspase-3 mRNA expression
41 celecoxib
42 cell growth
43 cell lines
44 cells
45 clean products
46 co-ordination enzyme
47 colorectal cancer cell lines
48 colorectal cancer cells
49 compounds
50 conditions
51 correlation
52 cyclooxygenase-2 inhibition
53 cyclooxygenase-2 inhibitor
54 derivatives
55 dose
56 effect
57 enzyme
58 expression
59 expression patterns
60 formation
61 gene expression
62 gene expression patterns
63 genes
64 growth
65 higher reaction rate
66 imidazole
67 imidazole derivatives
68 imidazolyl imidazole derivatives
69 imidazolyl imidazoles
70 increase
71 inhibition
72 inhibitors
73 irradiation
74 lines
75 mRNA expression
76 method
77 microarrays
78 microwave irradiation
79 microwave-assisted method
80 mild reaction conditions
81 novel Bisaryl imidazolyl imidazole derivatives
82 novel selective COX-2 inhibitor
83 organic reactions
84 pathway
85 patterns
86 products
87 proliferation
88 quantitative real-time PCR
89 rate
90 ratio
91 reaction
92 reaction conditions
93 reaction rate
94 real-time PCR
95 relation
96 relation of Bax
97 relationship
98 results
99 scientists
100 selective COX-2 inhibitors
101 study
102 synthetic compounds
103 time
104 treatment
105 schema:name Cyclooxygenase-2 inhibition by novel Bisaryl imidazolyl imidazole derivatives increases Bax/Bcl-2 ratio and upregulates Caspase-3 gene expression in Caco-2 colorectal cancer cell line
106 schema:pagination 199-204
107 schema:productId N8b0c7644374546ef90ad09d151c585ed
108 Nbdd93f1d602e4782a9ffbd753db05973
109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045853595
110 https://doi.org/10.1007/s13258-011-0168-0
111 schema:sdDatePublished 2022-01-01T18:27
112 schema:sdLicense https://scigraph.springernature.com/explorer/license/
113 schema:sdPublisher Ne535c7c4dee3456898a19310a35ed315
114 schema:url https://doi.org/10.1007/s13258-011-0168-0
115 sgo:license sg:explorer/license/
116 sgo:sdDataset articles
117 rdf:type schema:ScholarlyArticle
118 N38f25cd197934937bb425ca462e452f6 rdf:first sg:person.01153272413.66
119 rdf:rest N8fb4df74e11e4bf4a27684d17ae629af
120 N8b0c7644374546ef90ad09d151c585ed schema:name doi
121 schema:value 10.1007/s13258-011-0168-0
122 rdf:type schema:PropertyValue
123 N8e5e9ae45353415e915b59d0c7465722 schema:issueNumber 2
124 rdf:type schema:PublicationIssue
125 N8fb4df74e11e4bf4a27684d17ae629af rdf:first sg:person.01342306074.30
126 rdf:rest Nb51957276fcf43849f1831669890a38e
127 Na2f6d5c62a5040109790a20516382694 rdf:first sg:person.01245756105.18
128 rdf:rest rdf:nil
129 Nb20939f71bb0476db558a7b143902c34 rdf:first sg:person.0677401417.27
130 rdf:rest Na2f6d5c62a5040109790a20516382694
131 Nb51957276fcf43849f1831669890a38e rdf:first sg:person.01100253272.52
132 rdf:rest Nb20939f71bb0476db558a7b143902c34
133 Nbdd93f1d602e4782a9ffbd753db05973 schema:name dimensions_id
134 schema:value pub.1045853595
135 rdf:type schema:PropertyValue
136 Ne17fc76b63a843efaf5343b1d607eba2 schema:volumeNumber 34
137 rdf:type schema:PublicationVolume
138 Ne535c7c4dee3456898a19310a35ed315 schema:name Springer Nature - SN SciGraph project
139 rdf:type schema:Organization
140 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
141 schema:name Biological Sciences
142 rdf:type schema:DefinedTerm
143 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
144 schema:name Biochemistry and Cell Biology
145 rdf:type schema:DefinedTerm
146 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
147 schema:name Genetics
148 rdf:type schema:DefinedTerm
149 sg:journal.1040407 schema:issn 0254-5934
150 2092-9293
151 schema:name Genes & Genomics
152 schema:publisher Springer Nature
153 rdf:type schema:Periodical
154 sg:person.01100253272.52 schema:affiliation grid-institutes:grid.411583.a
155 schema:familyName Sankian
156 schema:givenName Mojtaba
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100253272.52
158 rdf:type schema:Person
159 sg:person.01153272413.66 schema:affiliation grid-institutes:grid.411583.a
160 schema:familyName Heravi
161 schema:givenName Reza Entezari
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153272413.66
163 rdf:type schema:Person
164 sg:person.01245756105.18 schema:affiliation grid-institutes:grid.411583.a
165 schema:familyName Behravan
166 schema:givenName Javad
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245756105.18
168 rdf:type schema:Person
169 sg:person.01342306074.30 schema:affiliation grid-institutes:grid.411583.a
170 schema:familyName Hadizadeh
171 schema:givenName Farzin
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342306074.30
173 rdf:type schema:Person
174 sg:person.0677401417.27 schema:affiliation grid-institutes:grid.411583.a
175 schema:familyName Afshari
176 schema:givenName Jalil Tavakol
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677401417.27
178 rdf:type schema:Person
179 sg:pub.10.1007/s00011-009-0034-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025968774
180 https://doi.org/10.1007/s00011-009-0034-6
181 rdf:type schema:CreativeWork
182 sg:pub.10.1038/348334a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005542034
183 https://doi.org/10.1038/348334a0
184 rdf:type schema:CreativeWork
185 sg:pub.10.1038/sj.onc.1210113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025777644
186 https://doi.org/10.1038/sj.onc.1210113
187 rdf:type schema:CreativeWork
188 grid-institutes:grid.411583.a schema:alternateName Department of Medicinal Chemistry, Mashhad University of Medical Sciences, Mashhad, Iran
189 Department of Pharmaceutical Biotechnology, Mashhad University of Medical Sciences, Mashhad, Iran
190 Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
191 schema:name Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
192 Department of Medicinal Chemistry, Mashhad University of Medical Sciences, Mashhad, Iran
193 Department of Pharmaceutical Biotechnology, Mashhad University of Medical Sciences, Mashhad, Iran
194 Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
195 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...