Identification and validation of the wound and insect bite early inducible promoter from Arabidopsis thaliana View Full Text


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Article Info

DATE

2022-02-21

AUTHORS

Devesh Kumar Mishra, Rakesh Srivastava, Bhoopendra K. Pandey, Praveen Chandra Verma, Samir Vishwanath Sawant

ABSTRACT

A wound-inducible promoter facilitates the regulated gene expression at the targeted site during the time of mechanical stress or infestation by the pathogen. The present work has aimed to identify a wound-inducible promoter that expresses at early time points preceding wound-stress treatment in Arabidopsis thaliana. The computational analysis of microarray data (GSE5627) resulted in the identification of five early inducible genes, viz., AT1G17380, AT1G80440, AT2G43530, AT3G48360, and AT5G13220. The RT-PCR analysis showed AT5G13220 (JASMONATE-ASSOCIATED 1) gene induced at a significantly higher level post 30 min of wounding. Thus, the promoter of the highly induced and early expressed wound-inducible gene, AT5G13220 (named PW220), was characterized by fusing with β-glucuronidase (gusA) reporter or Cry1EC genes. The fluorometric analysis and histochemical staining of the gusA gene and quantitative estimation of Cry1EC protein in Nicotiana tabacum transgenic lines confirmed wound-induced expression characteristic of the selected promoter. Insect bioassay suggested that wound-inducible and constitutive expression of Cry1EC protein in transgenic lines showed a similar level of protection against different instar Spodoptera litura larvae. Furthermore, we identified that abscisic acid influenced the wound-specific expression of the selected PW220 promoter in the transgenic lines, which correlates with the presence of conserved cis-regulatory elements associated with dehydration and abscisic acid responses. Altogether, our results suggested that the wound-inducible promoter PW220 provides an excellent alternative for developing insect-tolerant transgenic crops in the future. More... »

PAGES

74

References to SciGraph publications

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  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 2000-02. Rapid systemic accumulation of transcripts encoding a tobacco WRKY transcription factor upon wounding in MOLECULAR GENETICS AND GENOMICS
  • 2004-08-31. Problems in monitoring horizontal gene transfer in field trials of transgenic plants in NATURE BIOTECHNOLOGY
  • 1986-11. Regulation of synthesis of proteinase inhibitors I and II mRNAs in leaves of wounded tomato plants in PLANTA
  • 2017-12-01. Plant Biosynthetic Engineering Through Transcription Regulation: An Insight into Molecular Mechanisms During Environmental Stress in BIOSYNTHETIC TECHNOLOGY AND ENVIRONMENTAL CHALLENGES
  • 2006-08. Expression of the cry9Aa2 B.t. gene in tobacco chloroplasts confers resistance to potato tuber moth in TRANSGENIC RESEARCH
  • 2004-12. Characterization and activity enhancement of the phloem-specific pumpkin PP2 gene promoter in TRANSGENIC RESEARCH
  • 2011-10-09. Efficacy of genetically modified Bt toxins against insects with different genetic mechanisms of resistance in NATURE BIOTECHNOLOGY
  • 2007-02-24. Characterization of vascular-specific RSs1 and rolC promoters for their utilization in engineering plants to develop resistance against hemipteran insect pests in PLANTA
  • 2007-03-13. New Analysis of a Rat Feeding Study with a Genetically Modified Maize Reveals Signs of Hepatorenal Toxicity in ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
  • 2007-08-02. Testing of a heterologous, wound- and insect-inducible promoter for functional genomics studies in conifer defense in PLANT CELL REPORTS
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    http://dx.doi.org/10.1007/s13205-022-03143-0

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/35251877


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