Biosynthesis of l-phosphinothricin with enzymes from chromosomal integrated expression in E. coli View Full Text


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Article Info

DATE

2021-10-28

AUTHORS

Yu-Ke Cen, Huo Gong, Ya-Ping Xue, Yu-Guo Zheng

ABSTRACT

Phosphinothricin (PPT) is one of the most prevalently using herbicides. The commercial phosphinothricin products are generally in the form of a racemic mixture, of which only the l-phosphinothricin (L-PPT) gives herbicidal function. Synthesis of optically pure L-PPT by deracemization of D/L-PPT is a promising way to cut down the environmental burden and manufacturing cost. To convert D/L-PPT to L-PPT, we expressed the catalytic enzymes by genomic integration in E. coli. The whole production was implemented in two steps in one pot using four catalytic enzymes, namely d-amino acid oxidase, catalase, glutamate dehydrogenase, and glucose dehydrogenase. Finally, after a series of process optimization, the results showed that with our system the overall L-PPT yield reached 86%. Our study demonstrated a new strategy for L-PPT synthesis, based on enzymes from chromosomal integrated expression, which does not depend on antibiotic selection, and shows a high potential for future industrial application. More... »

PAGES

477

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13205-021-03037-7

DOI

http://dx.doi.org/10.1007/s13205-021-03037-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1142237408

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/34777934


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