Development of a fermentation strategy to enhance the catalytic efficiency of recombinant Escherichia coli for l-2-aminobutyric acid production View Full Text


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Article Info

DATE

2021-07-28

AUTHORS

Jian-Miao Xu, Ming Wang, Yi-Hua Jin, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

Microbial fermentation for enzyme production and then whole-cell catalysis for l-2-aminobutyric acid (l-ABA) production have huge potential for industrial application, but the catalytic capacities of cells are directly related to the fermentation process. Using a 50 L fermenter, the effects of initial glycerol concentration in the medium and rotating speed on cell catalytic capacity were investigated. Fermentation cells showed the best catalytic activity when the initial glycerol concentration was 12 g/L and the rotating speed was 250 rpm. Furthermore, we studied the difference between glycerol and glycerol mixtures as fed-batch media in pH–stat fed-batch fermentation. Results showed that glycerol had better catalytic activity than the glycerol mixture, and the effect of fed-batch fermentation was better than batch fermentation. Meanwhile, the enzyme activities of leucine dehydrogenase and formate dehydrogenase reached 129.87 U/g DCW and 437.02 U/g DCW, respectively, and the intracellular NAD(H) concentration reached 14.94 μmol/g DCW. Using the optimized fermentation parameters, amplified fermentation was then carried out in a 5000 L fermenter to demonstrate the industrial production of l-ABA by Escherichia coli BL21. More... »

PAGES

387

References to SciGraph publications

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  • 2019-02-28. Fermentative production of the unnatural amino acid l-2-aminobutyric acid based on metabolic engineering in MICROBIAL CELL FACTORIES
  • 2004-04-29. High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat in BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • 2018-09-27. Increased productivity of l-2-aminobutyric acid and total turnover number of NAD+/NADH in a one-pot system through enhanced thermostability of l-threonine deaminase in BIOTECHNOLOGY LETTERS
  • 2013-12-10. A one-pot system for production of l-2-aminobutyric acid from l-threonine by l-threonine deaminase and a NADH-regeneration system based on l-leucine dehydrogenase and formate dehydrogenase in BIOTECHNOLOGY LETTERS
  • 2011-02-09. Removal of l-alanine from the production of l-2-aminobutyric acid by introduction of alanine racemase and d-amino acid oxidase in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2001-01-11. Industrial biocatalysis today and tomorrow in NATURE
  • 2000-06. Investigating expression systems for the stable large-scale production of recombinant L-leucine-dehydrogenase from Bacillus cereus in Escherichia coli in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2004-02-19. High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat in BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • 2013-05-07. Achieving pH control in microalgal cultures through fed-batch addition of stoichiometrically-balanced growth media in BMC BIOTECHNOLOGY
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    http://scigraph.springernature.com/pub.10.1007/s13205-021-02937-y

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    http://dx.doi.org/10.1007/s13205-021-02937-y

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/34350092


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