Ontology type: schema:ScholarlyArticle
2021-07-28
AUTHORSJian-Miao Xu, Ming Wang, Yi-Hua Jin, Zhi-Qiang Liu, Yu-Guo Zheng
ABSTRACTMicrobial fermentation for enzyme production and then whole-cell catalysis for l-2-aminobutyric acid (l-ABA) production have huge potential for industrial application, but the catalytic capacities of cells are directly related to the fermentation process. Using a 50 L fermenter, the effects of initial glycerol concentration in the medium and rotating speed on cell catalytic capacity were investigated. Fermentation cells showed the best catalytic activity when the initial glycerol concentration was 12 g/L and the rotating speed was 250 rpm. Furthermore, we studied the difference between glycerol and glycerol mixtures as fed-batch media in pH–stat fed-batch fermentation. Results showed that glycerol had better catalytic activity than the glycerol mixture, and the effect of fed-batch fermentation was better than batch fermentation. Meanwhile, the enzyme activities of leucine dehydrogenase and formate dehydrogenase reached 129.87 U/g DCW and 437.02 U/g DCW, respectively, and the intracellular NAD(H) concentration reached 14.94 μmol/g DCW. Using the optimized fermentation parameters, amplified fermentation was then carried out in a 5000 L fermenter to demonstrate the industrial production of l-ABA by Escherichia coli BL21. More... »
PAGES387
http://scigraph.springernature.com/pub.10.1007/s13205-021-02937-y
DOIhttp://dx.doi.org/10.1007/s13205-021-02937-y
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1139988795
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/34350092
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Microbiology",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"id": "http://www.grid.ac/institutes/grid.469325.f",
"name": [
"Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
],
"type": "Organization"
},
"familyName": "Xu",
"givenName": "Jian-Miao",
"id": "sg:person.0661257657.83",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661257657.83"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"id": "http://www.grid.ac/institutes/grid.469325.f",
"name": [
"Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
],
"type": "Organization"
},
"familyName": "Wang",
"givenName": "Ming",
"id": "sg:person.011376201317.62",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011376201317.62"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"id": "http://www.grid.ac/institutes/grid.469325.f",
"name": [
"Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
],
"type": "Organization"
},
"familyName": "Jin",
"givenName": "Yi-Hua",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"id": "http://www.grid.ac/institutes/grid.469325.f",
"name": [
"Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
],
"type": "Organization"
},
"familyName": "Liu",
"givenName": "Zhi-Qiang",
"id": "sg:person.01256734541.85",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"id": "http://www.grid.ac/institutes/grid.469325.f",
"name": [
"Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China",
"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
],
"type": "Organization"
},
"familyName": "Zheng",
"givenName": "Yu-Guo",
"id": "sg:person.01144225405.06",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s00449-003-0347-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026602503",
"https://doi.org/10.1007/s00449-003-0347-8"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00253-011-3127-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023001854",
"https://doi.org/10.1007/s00253-011-3127-4"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10529-013-1424-y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038714378",
"https://doi.org/10.1007/s10529-013-1424-y"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s002030050482",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017448531",
"https://doi.org/10.1007/s002030050482"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10529-018-2607-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1107261511",
"https://doi.org/10.1007/s10529-018-2607-3"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00449-004-0361-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050202728",
"https://doi.org/10.1007/s00449-004-0361-5"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s002539900290",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001214899",
"https://doi.org/10.1007/s002539900290"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s12934-019-1095-z",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1112462281",
"https://doi.org/10.1186/s12934-019-1095-z"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35051736",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004489868",
"https://doi.org/10.1038/35051736"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1472-6750-13-39",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018751821",
"https://doi.org/10.1186/1472-6750-13-39"
],
"type": "CreativeWork"
}
],
"datePublished": "2021-07-28",
"datePublishedReg": "2021-07-28",
"description": "Microbial fermentation for enzyme production and then whole-cell catalysis for l-2-aminobutyric acid (l-ABA) production have huge potential for industrial application, but the catalytic capacities of cells are directly related to the fermentation process. Using a 50\u00a0L fermenter, the effects of initial glycerol concentration in the medium and rotating speed on cell catalytic capacity were investigated. Fermentation cells showed the best catalytic activity when the initial glycerol concentration was 12\u00a0g/L and the rotating speed was 250\u00a0rpm. Furthermore, we studied the difference between glycerol and glycerol mixtures as fed-batch media in pH\u2013stat fed-batch fermentation. Results showed that glycerol had better catalytic activity than the glycerol mixture, and the effect of fed-batch fermentation was better than batch fermentation. Meanwhile, the enzyme activities of leucine dehydrogenase and formate dehydrogenase reached 129.87 U/g DCW and 437.02 U/g DCW, respectively, and the intracellular NAD(H) concentration reached 14.94\u00a0\u03bcmol/g DCW. Using the optimized fermentation parameters, amplified fermentation was then carried out in a 5000\u00a0L fermenter to demonstrate the industrial production of l-ABA by Escherichia coli BL21.",
"genre": "article",
"id": "sg:pub.10.1007/s13205-021-02937-y",
"inLanguage": "en",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1045498",
"issn": [
"2190-572X",
"2190-5738"
],
"name": "3 Biotech",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "8",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "11"
}
],
"keywords": [
"fed-batch fermentation",
"Escherichia coli BL21",
"recombinant Escherichia coli",
"acid production",
"catalytic capacity",
"optimized fermentation parameters",
"microbial fermentation",
"coli BL21",
"formate dehydrogenase",
"Escherichia coli",
"leucine dehydrogenase",
"pH-stat fed-batch fermentation",
"enzyme production",
"fermentation strategy",
"enzyme activity",
"fermentation process",
"catalytic efficiency",
"whole-cell catalysis",
"fermentation",
"fermentation parameters",
"dehydrogenase",
"initial glycerol concentration",
"catalytic activity",
"DCW",
"cells",
"ABA",
"fermenter",
"good catalytic activity",
"BL21",
"production",
"coli",
"activity",
"intracellular",
"glycerol",
"glycerol concentration",
"industrial production",
"medium",
"concentration",
"catalysis",
"huge potential",
"industrial applications",
"capacity",
"development",
"effect",
"potential",
"process",
"strategies",
"glycerol mixtures",
"differences",
"mixture",
"results",
"efficiency",
"applications",
"speed",
"rpm",
"rotating speed",
"parameters",
"cell catalytic capacity",
"Fermentation cells",
"fed-batch media"
],
"name": "Development of a fermentation strategy to enhance the catalytic efficiency of recombinant Escherichia coli for l-2-aminobutyric acid production",
"pagination": "387",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1139988795"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s13205-021-02937-y"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"34350092"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s13205-021-02937-y",
"https://app.dimensions.ai/details/publication/pub.1139988795"
],
"sdDataset": "articles",
"sdDatePublished": "2022-01-01T18:59",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_908.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s13205-021-02937-y"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s13205-021-02937-y'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s13205-021-02937-y'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s13205-021-02937-y'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s13205-021-02937-y'
This table displays all metadata directly associated to this object as RDF triples.
191 TRIPLES
22 PREDICATES
96 URIs
78 LITERALS
7 BLANK NODES