Enhancement of gibberellic acid production from Fusarium fujikuroi by mutation breeding and glycerol addition View Full Text


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Article Info

DATE

2020-06-20

AUTHORS

Xiao-Lun Peng, Wei-Jun Zhao, Yuan-Shan Wang, Ke-Lei Dai, Yu-Ke Cen, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

Gibberellic acid (GA3) is a natural plant growth hormone that has been widely used in agriculture and horticulture. To obtain higher GA3 producing strains, the method of screening the strains for resistance to simvastatin was used after treatment with nitrosoguanidine (NTG) and gamma rays. The rationale for the strategy was that mutants showing simvastatin resistance were likely to be high GA3 producers, as their activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is relatively more effective. GA3 yield of mutant S109 increased by 14.2% than that of the original strain. The GA3 production ability in mutant S109 remained relatively stable after ten generations. With the addition of 0.4 g glycerol on the 5th day during the fermentation process in Erlenmeyer flask, maximum GA3 production of 2.7 g/L was achieved by this mutant, exhibiting 28.6% increase compared with original strain. Furthermore, we also achieved 2.8 g/L GA3 and had a 33.3% increase with addition 20 g glycerol on the 5th day during the fermentation process in a 5-L bioreactor. Our results indicated efficient GA3 production could be achieved on the condition that the supply of glycerol at the suitable conditions. This study would lay a foundation for industrial production of GA3. More... »

PAGES

312

References to SciGraph publications

  • 2012-12-05. Optimization of lipid production by the oleaginous yeast Lipomyces starkeyi by random mutagenesis coupled to cerulenin screening in AMB EXPRESS
  • 2017-12-04. Secondary metabolism in Fusarium fujikuroi: strategies to unravel the function of biosynthetic pathways in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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  • 1997-01. 3-Hydroxy-3-methylglutaryl-CoA reductase gene of Gibberella fujikuroi: isolation and characterization in CURRENT GENETICS
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  • 2002-06-11. A carotenoid biosynthesis gene cluster in Fusarium fujikuroi: the genes carB and carRA in MOLECULAR GENETICS AND GENOMICS
  • 2016-10-03. Toward glycerol biorefinery: metabolic engineering for the production of biofuels and chemicals from glycerol in BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
  • 2004-02-13. Biotechnological advantages of laboratory-scale solid-state fermentation with fungi in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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  • 2016-09-19. Kinetic analysis of the uptake of glucose and corn oil used as carbon sources in batch cultures of Gibberella fujikuroi in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 2013-01-25. Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli in MICROBIAL CELL FACTORIES
  • 2013-05-01. Characterization of novel mutants with an altered gibberellin spectrum in comparison to different wild-type strains of Fusarium fujikuroi in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1999-02. Isolation, characterization and disruption of the areA nitrogen regulatory gene of Gibberella fujikuroi in MOLECULAR GENETICS AND GENOMICS
  • <error retrieving object. in <ERROR RETRIEVING OBJECT
  • 2010-11-11. Inductive effect produced by a mixture of carbon source in the production of gibberellic acid by Gibberella fujikuroi in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 2018-08-01. Current advances in gibberellic acid (GA3) production, patented technologies and potential applications in PLANTA
  • 2001-12. Gibberellin Biosynthesis in Plants and Fungi: A Case of Convergent Evolution? in JOURNAL OF PLANT GROWTH REGULATION
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s13205-020-02303-4

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    http://dx.doi.org/10.1007/s13205-020-02303-4

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/32582509


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    61 growth hormone
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    63 hormone
    64 horticulture
    65 increase
    66 industrial production
    67 method
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    70 natural plant growth hormones
    71 nitrosoguanidine
    72 original strain
    73 plant growth hormones
    74 process
    75 producers
    76 production
    77 production ability
    78 rationale
    79 rays
    80 reductase
    81 resistance
    82 results
    83 simvastatin
    84 simvastatin resistance
    85 strains
    86 strategies
    87 study
    88 suitable conditions
    89 supply
    90 supply of glycerol
    91 treatment
    92 yield
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