Enhanced L-methionine production by genetically engineered Escherichia coli through fermentation optimization View Full Text


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Article Info

DATE

2019-02-19

AUTHORS

Hai-Yan Zhou, Wang-Jie Wu, Kun Niu, Yue-Ying Xu, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

Microbial fermentation for L-methionine (L-Met) production based on natural renewable resources is attractive and challenging. In this work, the effects of medium composition and fermentation conditions were investigated to improve L-Met production by genetically engineered Escherichia coli MET-3. Statistical optimization techniques including Plackett–Burman (PB) design and Box–Behnken design (BBD) were adopted first to optimize the culture medium. Results of PB-designed experiments indicated that the culture medium components including glucose, yeast extract, KH2PO4, and MgSO4.7H2O had significant effects on L-Met biosynthesis. With their best-predicted concentration established by BBD (glucose 37.43 g/L, yeast extract 0.95 g/L, KH2PO4 1.82 g/L, and MgSO4.7H2O 4.51 g/L), L-Met titer was increased to 3.04 g/L from less than 2.0 g/L. For further enhancement of L-Met biosynthesis, the fermentation conditions of batch cultivation carried out in a 5-L fermentor were optimized, and the optimum results were obtained at an agitation rate of 300 rpm, medium pH of 7.0, and induction temperature of 28 °C. Based on the optimization parameters, fed-batch fermentation with the modified medium was conducted. As a result, great improvement of L-Met titer (12.80 g/L) and yield (0.13 mol/mol) were achieved, with an increase of 38.53% and 30.0% compared with those of the basal medium, respectively. Furthermore, higher L-Met productivity of 0.261 g/L/h was obtained, representing 2.13-fold higher in comparison to the original medium. The results may provide a helpful reference for further study on strain improvement and fermentation control. More... »

PAGES

96

References to SciGraph publications

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    http://scigraph.springernature.com/pub.10.1007/s13205-019-1609-8

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    http://dx.doi.org/10.1007/s13205-019-1609-8

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30800607


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    54 fermentation
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    66 medium
    67 medium components
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    74 optimization techniques
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    76 original medium
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    78 parameters
    79 production
    80 productivity
    81 rate
    82 reference
    83 renewable resources
    84 resources
    85 results
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    87 significant effect
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    89 strain improvement
    90 study
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    93 titers
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