Metabolic engineering of E. coli for the production of O-succinyl-l-homoserine with high yield View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-07-09

AUTHORS

Jian-Feng Huang, Bo Zhang, Zhen-Yang Shen, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

O-succinyl-l-homoserine (OSH) is a promising platform chemical for the production of C4 chemicals with huge market potential which can be produced by fermentation from glucose. To construct a strain capable of producing OSH with high yield, the metJ (encodes transcriptional repressor) and metI (encodes a subunit of dl-methionine transporter) were deleted in Escherichia coli W3110 to obtain a strain E. coli ∆JI. Then, overexpression of metL (encodes bifunctional aspartate kinase/homoserine dehydrogenase II) and inactivation of metB (encodes cystathionine γ-synthase) were implemented in one step, and the OSH titer of the resulting strain E. coli ∆JIB* TrcmetL was dramatically increased to 7.30 g/L. The feedback regulation was further relieved by progressively overexpressing metAfbr (encodes homoserine O-succinyltransferase), yjeH (encodes l-methionine exporter), and thrAfbr (encodes bifunctional aspartate kinase/homoserine dehydrogenase I) to increase the metabolic flux from aspartate to OSH. The 100% rationally designed strain E. coli ∆JIB* TrcmetL/pTrc-metAfbr-Trc-thrAfbr-yjeH produced 9.31 g/L OSH from 20 g/L glucose (0.466 g/g glucose) in batch fermentation, which represents the highest OSH yield from glucose reported to date. The culture profiles of the newly constructed strains were recorded to investigate their productive properties. The effects of l-methionine addition on the fermentation process of the optimal strain were also studied. Our results demonstrate that tuning the expression level of metL, inactivation of metB, and attenuation of feedback resistance of the crucial enzymes in the biosynthetic pathway are the key factors that impact the OSH production in E. coli. More... »

PAGES

310

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13205-018-1332-x

DOI

http://dx.doi.org/10.1007/s13205-018-1332-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105434087

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30002999


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huang", 
        "givenName": "Jian-Feng", 
        "id": "sg:person.01112216671.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01112216671.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Bo", 
        "id": "sg:person.016106244405.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016106244405.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shen", 
        "givenName": "Zhen-Yang", 
        "id": "sg:person.01164160502.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164160502.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Zhi-Qiang", 
        "id": "sg:person.01256734541.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zheng", 
        "givenName": "Yu-Guo", 
        "id": "sg:person.01144225405.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1385/1-59259-847-1:179", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000897443", 
          "https://doi.org/10.1385/1-59259-847-1:179"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-016-8083-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050459052", 
          "https://doi.org/10.1007/s00253-016-8083-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13205-016-0365-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049871013", 
          "https://doi.org/10.1007/s13205-016-0365-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12866-017-0988-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084250158", 
          "https://doi.org/10.1186/s12866-017-0988-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/10_2016_30", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027991643", 
          "https://doi.org/10.1007/10_2016_30"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13205-014-0252-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045645337", 
          "https://doi.org/10.1007/s13205-014-0252-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-014-1499-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053708726", 
          "https://doi.org/10.1007/s10295-014-1499-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-014-6156-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012328554", 
          "https://doi.org/10.1007/s00253-014-6156-y"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-07-09", 
    "datePublishedReg": "2018-07-09", 
    "description": "O-succinyl-l-homoserine (OSH) is a promising platform chemical for the production of C4 chemicals with huge market potential which can be produced by fermentation from glucose. To construct a strain capable of producing OSH with high yield, the metJ (encodes transcriptional repressor) and metI (encodes a subunit of dl-methionine transporter) were deleted in Escherichia coli W3110 to obtain a strain E. coli \u2206JI. Then, overexpression of metL (encodes bifunctional aspartate kinase/homoserine dehydrogenase II) and inactivation of metB (encodes cystathionine \u03b3-synthase) were implemented in one step, and the OSH titer of the resulting strain E. coli \u2206JIB* TrcmetL was dramatically increased to 7.30\u00a0g/L. The feedback regulation was further relieved by progressively overexpressing metAfbr (encodes homoserine O-succinyltransferase), yjeH (encodes l-methionine exporter), and thrAfbr (encodes bifunctional aspartate kinase/homoserine dehydrogenase I) to increase the metabolic flux from aspartate to OSH. The 100% rationally designed strain E. coli \u2206JIB* TrcmetL/pTrc-metAfbr-Trc-thrAfbr-yjeH produced 9.31\u00a0g/L OSH from 20\u00a0g/L glucose (0.466\u00a0g/g glucose) in batch fermentation, which represents the highest OSH yield from glucose reported to date. The culture profiles of the newly constructed strains were recorded to investigate their productive properties. The effects of l-methionine addition on the fermentation process of the optimal strain were also studied. Our results demonstrate that tuning the expression level of metL, inactivation of metB, and attenuation of feedback resistance of the crucial enzymes in the biosynthetic pathway are the key factors that impact the OSH production in E. coli.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s13205-018-1332-x", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.8198364", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045498", 
        "issn": [
          "2190-572X", 
          "2190-5738"
        ], 
        "name": "3 Biotech", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "keywords": [
      "strain E. coli", 
      "Escherichia coli W3110", 
      "metabolic engineering", 
      "biosynthetic pathway", 
      "metabolic flux", 
      "coli W3110", 
      "crucial enzyme", 
      "feedback regulation", 
      "expression levels", 
      "coli", 
      "metB", 
      "promising platform chemical", 
      "homoserine", 
      "fermentation process", 
      "methionine addition", 
      "batch fermentation", 
      "C4 chemicals", 
      "inactivation", 
      "MetJ", 
      "fermentation", 
      "strains", 
      "optimal strain", 
      "metls", 
      "W3110", 
      "huge market potential", 
      "overexpression", 
      "high yield", 
      "platform chemicals", 
      "production", 
      "enzyme", 
      "regulation", 
      "pathway", 
      "yield", 
      "glucose", 
      "aspartate", 
      "chemicals", 
      "productive properties", 
      "key factors", 
      "market potential", 
      "resistance", 
      "feedback resistance", 
      "levels", 
      "date", 
      "engineering", 
      "addition", 
      "factors", 
      "potential", 
      "profile", 
      "step", 
      "METI", 
      "process", 
      "effect", 
      "flux", 
      "properties", 
      "titers", 
      "results", 
      "attenuation", 
      "OSH", 
      "culture profiles"
    ], 
    "name": "Metabolic engineering of E. coli for the production of O-succinyl-l-homoserine with high yield", 
    "pagination": "310", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1105434087"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s13205-018-1332-x"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30002999"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s13205-018-1332-x", 
      "https://app.dimensions.ai/details/publication/pub.1105434087"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:34", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_771.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s13205-018-1332-x"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s13205-018-1332-x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s13205-018-1332-x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s13205-018-1332-x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s13205-018-1332-x'


 

This table displays all metadata directly associated to this object as RDF triples.

184 TRIPLES      22 PREDICATES      93 URIs      77 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s13205-018-1332-x schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N0c68b21d76f7471c89d67f33b66f5e96
4 schema:citation sg:pub.10.1007/10_2016_30
5 sg:pub.10.1007/s00253-014-6156-y
6 sg:pub.10.1007/s00253-016-8083-6
7 sg:pub.10.1007/s10295-014-1499-z
8 sg:pub.10.1007/s13205-014-0252-7
9 sg:pub.10.1007/s13205-016-0365-2
10 sg:pub.10.1186/s12866-017-0988-4
11 sg:pub.10.1385/1-59259-847-1:179
12 schema:datePublished 2018-07-09
13 schema:datePublishedReg 2018-07-09
14 schema:description O-succinyl-l-homoserine (OSH) is a promising platform chemical for the production of C4 chemicals with huge market potential which can be produced by fermentation from glucose. To construct a strain capable of producing OSH with high yield, the metJ (encodes transcriptional repressor) and metI (encodes a subunit of dl-methionine transporter) were deleted in Escherichia coli W3110 to obtain a strain E. coli ∆JI. Then, overexpression of metL (encodes bifunctional aspartate kinase/homoserine dehydrogenase II) and inactivation of metB (encodes cystathionine γ-synthase) were implemented in one step, and the OSH titer of the resulting strain E. coli ∆JIB* TrcmetL was dramatically increased to 7.30 g/L. The feedback regulation was further relieved by progressively overexpressing metAfbr (encodes homoserine O-succinyltransferase), yjeH (encodes l-methionine exporter), and thrAfbr (encodes bifunctional aspartate kinase/homoserine dehydrogenase I) to increase the metabolic flux from aspartate to OSH. The 100% rationally designed strain E. coli ∆JIB* TrcmetL/pTrc-metAfbr-Trc-thrAfbr-yjeH produced 9.31 g/L OSH from 20 g/L glucose (0.466 g/g glucose) in batch fermentation, which represents the highest OSH yield from glucose reported to date. The culture profiles of the newly constructed strains were recorded to investigate their productive properties. The effects of l-methionine addition on the fermentation process of the optimal strain were also studied. Our results demonstrate that tuning the expression level of metL, inactivation of metB, and attenuation of feedback resistance of the crucial enzymes in the biosynthetic pathway are the key factors that impact the OSH production in E. coli.
15 schema:genre article
16 schema:inLanguage en
17 schema:isAccessibleForFree true
18 schema:isPartOf N319a11f391254d4ea1c90dfa91938acb
19 N79af26a940e542a1879f4ac42a35973f
20 sg:journal.1045498
21 schema:keywords C4 chemicals
22 Escherichia coli W3110
23 METI
24 MetJ
25 OSH
26 W3110
27 addition
28 aspartate
29 attenuation
30 batch fermentation
31 biosynthetic pathway
32 chemicals
33 coli
34 coli W3110
35 crucial enzyme
36 culture profiles
37 date
38 effect
39 engineering
40 enzyme
41 expression levels
42 factors
43 feedback regulation
44 feedback resistance
45 fermentation
46 fermentation process
47 flux
48 glucose
49 high yield
50 homoserine
51 huge market potential
52 inactivation
53 key factors
54 levels
55 market potential
56 metB
57 metabolic engineering
58 metabolic flux
59 methionine addition
60 metls
61 optimal strain
62 overexpression
63 pathway
64 platform chemicals
65 potential
66 process
67 production
68 productive properties
69 profile
70 promising platform chemical
71 properties
72 regulation
73 resistance
74 results
75 step
76 strain E. coli
77 strains
78 titers
79 yield
80 schema:name Metabolic engineering of E. coli for the production of O-succinyl-l-homoserine with high yield
81 schema:pagination 310
82 schema:productId N25d06740f88b454fba3b2f39dba444b6
83 N9ca87368481d46c2a11899c473dae25a
84 Ne50d3c7b09c24c08816c9200b3ea52af
85 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105434087
86 https://doi.org/10.1007/s13205-018-1332-x
87 schema:sdDatePublished 2022-05-20T07:34
88 schema:sdLicense https://scigraph.springernature.com/explorer/license/
89 schema:sdPublisher Nfd42b5ef31194c749d110db44057ef69
90 schema:url https://doi.org/10.1007/s13205-018-1332-x
91 sgo:license sg:explorer/license/
92 sgo:sdDataset articles
93 rdf:type schema:ScholarlyArticle
94 N0c68b21d76f7471c89d67f33b66f5e96 rdf:first sg:person.01112216671.83
95 rdf:rest Na81059b5a1a64e568a59ed77904ad069
96 N25d06740f88b454fba3b2f39dba444b6 schema:name dimensions_id
97 schema:value pub.1105434087
98 rdf:type schema:PropertyValue
99 N319a11f391254d4ea1c90dfa91938acb schema:volumeNumber 8
100 rdf:type schema:PublicationVolume
101 N79af26a940e542a1879f4ac42a35973f schema:issueNumber 7
102 rdf:type schema:PublicationIssue
103 N9ca87368481d46c2a11899c473dae25a schema:name pubmed_id
104 schema:value 30002999
105 rdf:type schema:PropertyValue
106 Na81059b5a1a64e568a59ed77904ad069 rdf:first sg:person.016106244405.13
107 rdf:rest Ncb9b979d44654e7f86a857934c9ca1fd
108 Naf7f24b3a28546a684cd7da9150106ff rdf:first sg:person.01256734541.85
109 rdf:rest Nd98b3eba02eb42e69a734592b5ed5349
110 Ncb9b979d44654e7f86a857934c9ca1fd rdf:first sg:person.01164160502.99
111 rdf:rest Naf7f24b3a28546a684cd7da9150106ff
112 Nd98b3eba02eb42e69a734592b5ed5349 rdf:first sg:person.01144225405.06
113 rdf:rest rdf:nil
114 Ne50d3c7b09c24c08816c9200b3ea52af schema:name doi
115 schema:value 10.1007/s13205-018-1332-x
116 rdf:type schema:PropertyValue
117 Nfd42b5ef31194c749d110db44057ef69 schema:name Springer Nature - SN SciGraph project
118 rdf:type schema:Organization
119 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
120 schema:name Biological Sciences
121 rdf:type schema:DefinedTerm
122 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
123 schema:name Biochemistry and Cell Biology
124 rdf:type schema:DefinedTerm
125 sg:grant.8198364 http://pending.schema.org/fundedItem sg:pub.10.1007/s13205-018-1332-x
126 rdf:type schema:MonetaryGrant
127 sg:journal.1045498 schema:issn 2190-572X
128 2190-5738
129 schema:name 3 Biotech
130 schema:publisher Springer Nature
131 rdf:type schema:Periodical
132 sg:person.01112216671.83 schema:affiliation grid-institutes:grid.469325.f
133 schema:familyName Huang
134 schema:givenName Jian-Feng
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01112216671.83
136 rdf:type schema:Person
137 sg:person.01144225405.06 schema:affiliation grid-institutes:grid.469325.f
138 schema:familyName Zheng
139 schema:givenName Yu-Guo
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06
141 rdf:type schema:Person
142 sg:person.01164160502.99 schema:affiliation grid-institutes:grid.469325.f
143 schema:familyName Shen
144 schema:givenName Zhen-Yang
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164160502.99
146 rdf:type schema:Person
147 sg:person.01256734541.85 schema:affiliation grid-institutes:grid.469325.f
148 schema:familyName Liu
149 schema:givenName Zhi-Qiang
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85
151 rdf:type schema:Person
152 sg:person.016106244405.13 schema:affiliation grid-institutes:grid.469325.f
153 schema:familyName Zhang
154 schema:givenName Bo
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016106244405.13
156 rdf:type schema:Person
157 sg:pub.10.1007/10_2016_30 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027991643
158 https://doi.org/10.1007/10_2016_30
159 rdf:type schema:CreativeWork
160 sg:pub.10.1007/s00253-014-6156-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1012328554
161 https://doi.org/10.1007/s00253-014-6156-y
162 rdf:type schema:CreativeWork
163 sg:pub.10.1007/s00253-016-8083-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050459052
164 https://doi.org/10.1007/s00253-016-8083-6
165 rdf:type schema:CreativeWork
166 sg:pub.10.1007/s10295-014-1499-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1053708726
167 https://doi.org/10.1007/s10295-014-1499-z
168 rdf:type schema:CreativeWork
169 sg:pub.10.1007/s13205-014-0252-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045645337
170 https://doi.org/10.1007/s13205-014-0252-7
171 rdf:type schema:CreativeWork
172 sg:pub.10.1007/s13205-016-0365-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049871013
173 https://doi.org/10.1007/s13205-016-0365-2
174 rdf:type schema:CreativeWork
175 sg:pub.10.1186/s12866-017-0988-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084250158
176 https://doi.org/10.1186/s12866-017-0988-4
177 rdf:type schema:CreativeWork
178 sg:pub.10.1385/1-59259-847-1:179 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000897443
179 https://doi.org/10.1385/1-59259-847-1:179
180 rdf:type schema:CreativeWork
181 grid-institutes:grid.469325.f schema:alternateName Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China
182 schema:name Engineering Research Center of Bioconversion and Bio-purification, Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China
183 Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China
184 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...