Molecular characterization of carbapenemases of clinical Acinetobacter baumannii–calcoaceticus complex isolates from a University Hospital in Tunisia View Full Text


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Article Info

DATE

2018-07

AUTHORS

Hadhemi Ben Cheikh, Sara Domingues, Eduarda Silveira, Yosr Kadri, Natasha Rosário, Maha Mastouri, Gabriela Jorge Da Silva

ABSTRACT

The aim of this study was to identify the carbapenemases from clinical carbapenem-resistant Acinetobacter baumannii-calcoaceticus complex (CRABC) isolates and to assess their potential dissemination by conjugation and natural transformation. CRABC (n = 101) were collected consecutively from inpatients of the University Hospital of Monastir, Tunisia, from 2013 to 2016. Antimicrobial susceptibility was determined by the disk diffusion method and E-test. Carbapenemase-encoding genes were screened by PCR. Genotyping was performed by Pasteur MLST scheme. Isolates were resistant to all beta-lactams, fluoroquinolones and aminoglycosides while 80 and 90% were susceptible to tigecycline and colistin, respectively. Resistance and intermediate resistance to imipenem were 87 and 13%, respectively. The genes blaOXA-24-like, blaOXA-58-like, blaOXA-143-like, blaOXA-48-like, blaVIM, blaIMP, and blaKPC were not found. The blaOXA-51-like and blaOXA-23-like genes were present in 100 and 82.17% isolates, respectively. One isolate (< 1%) carried blaNDM-1 and blaOXA-51-like and belonged to Sequence Type 85 (ST85). Absence of transconjugants suggests a chromosomal location of NDM-1 determinant. The blaNDM-1 gene was inserted in a truncated form of Tn125, which may explain the absence of blaNDM-1 carrier-transformants. To our knowledge, this is the first report of the finding of NDM-positive A. baumannii in Tunisian territory. The study shows that despite the low prevalence and potential spread of NDM-1 enzyme among CRABC, continuous regional antimicrobial resistance surveillance and improved infection control measures are required in Tunisia to prevent further dissemination. More... »

PAGES

297

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13205-018-1310-3

DOI

http://dx.doi.org/10.1007/s13205-018-1310-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105143859

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29963357


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47 schema:description The aim of this study was to identify the carbapenemases from clinical carbapenem-resistant <i>Acinetobacter baumannii-calcoaceticus</i> complex (CRABC) isolates and to assess their potential dissemination by conjugation and natural transformation. CRABC (<i>n</i> = 101) were collected consecutively from inpatients of the University Hospital of Monastir, Tunisia, from 2013 to 2016. Antimicrobial susceptibility was determined by the disk diffusion method and E-test. Carbapenemase-encoding genes were screened by PCR. Genotyping was performed by Pasteur MLST scheme. Isolates were resistant to all beta-lactams, fluoroquinolones and aminoglycosides while 80 and 90% were susceptible to tigecycline and colistin, respectively. Resistance and intermediate resistance to imipenem were 87 and 13%, respectively. The genes <i>bla</i><sub>OXA-24</sub>-like, <i>bla</i><sub>OXA-58</sub>-like, <i>bla</i><sub>OXA-143</sub>-like, <i>bla</i><sub>OXA-48</sub>-like, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>IMP,</sub> and <i>bla</i><sub>KPC</sub> were not found. The <i>bla</i><sub>OXA-51</sub>-like and <i>bla</i><sub>OXA-23</sub>-like genes were present in 100 and 82.17% isolates, respectively. One isolate (&lt; 1%) carried <i>bla</i><sub>NDM-1</sub> and <i>bla</i><sub>OXA-51</sub>-like and belonged to Sequence Type 85 (ST85). Absence of transconjugants suggests a chromosomal location of NDM-1 determinant. The <i>bla</i><sub>NDM-1</sub> gene was inserted in a truncated form of Tn<i>125</i>, which may explain the absence of <i>bla</i><sub>NDM-1</sub> carrier-transformants. To our knowledge, this is the first report of the finding of NDM-positive <i>A. baumannii</i> in Tunisian territory. The study shows that despite the low prevalence and potential spread of NDM-1 enzyme among CRABC, continuous regional antimicrobial resistance surveillance and improved infection control measures are required in Tunisia to prevent further dissemination.
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