Cellulase production using natural medium and its application on enzymatic hydrolysis of thermo chemically pretreated biomass View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-06-21

AUTHORS

Shivani Sharma, Vinay Sharma, Arindam Kuila

ABSTRACT

Lignocellulosic bioethanol is an important renewable fuel for transportation purpose. Commercial production of lignocellulosic bioethanol mainly depends on cost of cellulase production, efficient pretreatment and enzymatic hydrolysis process. In the present study cellulase production from Aspergillus niger under submerged fermentation (SmF) was optimized using coconut water as natural medium. Maximum cellulase production (0.53 IU/mL) was achieved within 3 days of incubation using 8 % (w/v) waste paper and 0.07 % (w/v) glucose. The produced cellulase was applied for enzymatic hydrolysis of thermo chemically (dilute acid and alkaline) pretreated biomass (equal mixture of wheat straw and cotton stalk). Optimization of dilute acid and dilute alkaline pretreatment showed dilute alkaline pretreatment was more effective for higher reducing sugar production. Maximum reducing sugar yield of 398.0 mg/g dry biomass was obtained from dilute alkaline pretreated biomass (using 0.5 M sodium hydroxide, 8 % substrate concentration, 120 °C temperature and 20 min of incubation time). The presence of difference sugars (glucose, xylose, mannose, maltose) in the saccharified sample was confirmed by thin layer chromatographic analysis. The effectiveness of dilute alkaline pretreatment was further confirmed by biochemical composition (cellulose, hemicelluloses and lignin) and structural (furrier transformed infrared spectroscopic and scanning electron microscopic) analysis. The above result can be useful for commercial production of lignocellulosic bioethanol. More... »

PAGES

139

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13205-016-0465-z

DOI

http://dx.doi.org/10.1007/s13205-016-0465-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032088924

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28330211


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Bioscience and Biotechnology Department, Banasthali University, 304022, Banasthali, Rajasthan, India", 
          "id": "http://www.grid.ac/institutes/grid.440551.1", 
          "name": [
            "Bioscience and Biotechnology Department, Banasthali University, 304022, Banasthali, Rajasthan, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sharma", 
        "givenName": "Shivani", 
        "id": "sg:person.010375433167.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010375433167.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Bioscience and Biotechnology Department, Banasthali University, 304022, Banasthali, Rajasthan, India", 
          "id": "http://www.grid.ac/institutes/grid.440551.1", 
          "name": [
            "Bioscience and Biotechnology Department, Banasthali University, 304022, Banasthali, Rajasthan, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sharma", 
        "givenName": "Vinay", 
        "id": "sg:person.01010037420.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010037420.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Bioscience and Biotechnology Department, Banasthali University, 304022, Banasthali, Rajasthan, India", 
          "id": "http://www.grid.ac/institutes/grid.440551.1", 
          "name": [
            "Bioscience and Biotechnology Department, Banasthali University, 304022, Banasthali, Rajasthan, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kuila", 
        "givenName": "Arindam", 
        "id": "sg:person.012565754567.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012565754567.67"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/s13068-014-0179-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035594727", 
          "https://doi.org/10.1186/s13068-014-0179-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13068-015-0419-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030058694", 
          "https://doi.org/10.1186/s13068-015-0419-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10068-011-0178-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045267153", 
          "https://doi.org/10.1007/s10068-011-0178-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/2193-1801-3-92", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023090777", 
          "https://doi.org/10.1186/2193-1801-3-92"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1754-6834-6-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001199959", 
          "https://doi.org/10.1186/1754-6834-6-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/2193-1801-3-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020248080", 
          "https://doi.org/10.1186/2193-1801-3-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1754-6834-6-170", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013521029", 
          "https://doi.org/10.1186/1754-6834-6-170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1754-6834-6-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039804556", 
          "https://doi.org/10.1186/1754-6834-6-8"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-06-21", 
    "datePublishedReg": "2016-06-21", 
    "description": "Lignocellulosic bioethanol is an important renewable fuel for transportation purpose. Commercial production of lignocellulosic bioethanol mainly depends on cost of cellulase production, efficient pretreatment and enzymatic hydrolysis process. In the present study cellulase production from Aspergillus niger under submerged fermentation (SmF) was optimized using coconut water as natural medium. Maximum cellulase production (0.53\u00a0IU/mL) was achieved within 3\u00a0days of incubation using 8\u00a0% (w/v) waste paper and 0.07\u00a0% (w/v) glucose. The produced cellulase was applied for enzymatic hydrolysis of thermo chemically (dilute acid and alkaline) pretreated biomass (equal mixture of wheat straw and cotton stalk). Optimization of dilute acid and dilute alkaline pretreatment showed dilute alkaline pretreatment was more effective for higher reducing sugar production. Maximum reducing sugar yield of 398.0\u00a0mg/g dry biomass was obtained from dilute alkaline pretreated biomass (using 0.5\u00a0M sodium hydroxide, 8\u00a0% substrate concentration, 120\u00a0\u00b0C temperature and 20\u00a0min of incubation time). The presence of difference sugars (glucose, xylose, mannose, maltose) in the saccharified sample was confirmed by thin layer chromatographic analysis. The effectiveness of dilute alkaline pretreatment was further confirmed by biochemical composition (cellulose, hemicelluloses and lignin) and structural (furrier transformed infrared spectroscopic and scanning electron microscopic) analysis. The above result can be useful for commercial production of lignocellulosic bioethanol.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s13205-016-0465-z", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045498", 
        "issn": [
          "2190-572X", 
          "2190-5738"
        ], 
        "name": "3 Biotech", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "6"
      }
    ], 
    "keywords": [
      "dilute alkaline pretreatment", 
      "lignocellulosic bioethanol", 
      "alkaline pretreatment", 
      "important renewable fuel", 
      "waste paper", 
      "renewable fuels", 
      "enzymatic hydrolysis process", 
      "transportation purposes", 
      "efficient pretreatment", 
      "hydrolysis process", 
      "commercial production", 
      "thermo", 
      "natural media", 
      "bioethanol", 
      "dilute alkaline", 
      "enzymatic hydrolysis", 
      "fuel", 
      "dilute acid", 
      "sugar production", 
      "sugar yield", 
      "structural analysis", 
      "biomass", 
      "cellulase production", 
      "saccharified samples", 
      "optimization", 
      "dry biomass", 
      "water", 
      "applications", 
      "production", 
      "coconut water", 
      "above results", 
      "cost", 
      "process", 
      "submerged fermentation", 
      "medium", 
      "analysis", 
      "composition", 
      "alkaline", 
      "pretreatment", 
      "effectiveness", 
      "chromatographic analysis", 
      "results", 
      "hydrolysis", 
      "days of incubation", 
      "fermentation", 
      "samples", 
      "yield", 
      "maximum cellulase production", 
      "Aspergillus niger", 
      "purpose", 
      "presence", 
      "cellulase", 
      "biochemical composition", 
      "acid", 
      "thin-layer chromatographic analysis", 
      "sugars", 
      "niger", 
      "days", 
      "glucose", 
      "incubation", 
      "paper"
    ], 
    "name": "Cellulase production using natural medium and its application on enzymatic hydrolysis of thermo chemically pretreated biomass", 
    "pagination": "139", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1032088924"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s13205-016-0465-z"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28330211"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s13205-016-0465-z", 
      "https://app.dimensions.ai/details/publication/pub.1032088924"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:41", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_697.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s13205-016-0465-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s13205-016-0465-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s13205-016-0465-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s13205-016-0465-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s13205-016-0465-z'


 

This table displays all metadata directly associated to this object as RDF triples.

168 TRIPLES      21 PREDICATES      94 URIs      78 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s13205-016-0465-z schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N727819d571fd4ed99ea5cc85e0cf68c9
4 schema:citation sg:pub.10.1007/s10068-011-0178-3
5 sg:pub.10.1186/1754-6834-6-1
6 sg:pub.10.1186/1754-6834-6-170
7 sg:pub.10.1186/1754-6834-6-8
8 sg:pub.10.1186/2193-1801-3-1
9 sg:pub.10.1186/2193-1801-3-92
10 sg:pub.10.1186/s13068-014-0179-6
11 sg:pub.10.1186/s13068-015-0419-4
12 schema:datePublished 2016-06-21
13 schema:datePublishedReg 2016-06-21
14 schema:description Lignocellulosic bioethanol is an important renewable fuel for transportation purpose. Commercial production of lignocellulosic bioethanol mainly depends on cost of cellulase production, efficient pretreatment and enzymatic hydrolysis process. In the present study cellulase production from Aspergillus niger under submerged fermentation (SmF) was optimized using coconut water as natural medium. Maximum cellulase production (0.53 IU/mL) was achieved within 3 days of incubation using 8 % (w/v) waste paper and 0.07 % (w/v) glucose. The produced cellulase was applied for enzymatic hydrolysis of thermo chemically (dilute acid and alkaline) pretreated biomass (equal mixture of wheat straw and cotton stalk). Optimization of dilute acid and dilute alkaline pretreatment showed dilute alkaline pretreatment was more effective for higher reducing sugar production. Maximum reducing sugar yield of 398.0 mg/g dry biomass was obtained from dilute alkaline pretreated biomass (using 0.5 M sodium hydroxide, 8 % substrate concentration, 120 °C temperature and 20 min of incubation time). The presence of difference sugars (glucose, xylose, mannose, maltose) in the saccharified sample was confirmed by thin layer chromatographic analysis. The effectiveness of dilute alkaline pretreatment was further confirmed by biochemical composition (cellulose, hemicelluloses and lignin) and structural (furrier transformed infrared spectroscopic and scanning electron microscopic) analysis. The above result can be useful for commercial production of lignocellulosic bioethanol.
15 schema:genre article
16 schema:isAccessibleForFree true
17 schema:isPartOf N3602bc0321e747c1a534230f1303b990
18 N4f1a3f8c35c648f99ab542691a437d0f
19 sg:journal.1045498
20 schema:keywords Aspergillus niger
21 above results
22 acid
23 alkaline
24 alkaline pretreatment
25 analysis
26 applications
27 biochemical composition
28 bioethanol
29 biomass
30 cellulase
31 cellulase production
32 chromatographic analysis
33 coconut water
34 commercial production
35 composition
36 cost
37 days
38 days of incubation
39 dilute acid
40 dilute alkaline
41 dilute alkaline pretreatment
42 dry biomass
43 effectiveness
44 efficient pretreatment
45 enzymatic hydrolysis
46 enzymatic hydrolysis process
47 fermentation
48 fuel
49 glucose
50 hydrolysis
51 hydrolysis process
52 important renewable fuel
53 incubation
54 lignocellulosic bioethanol
55 maximum cellulase production
56 medium
57 natural media
58 niger
59 optimization
60 paper
61 presence
62 pretreatment
63 process
64 production
65 purpose
66 renewable fuels
67 results
68 saccharified samples
69 samples
70 structural analysis
71 submerged fermentation
72 sugar production
73 sugar yield
74 sugars
75 thermo
76 thin-layer chromatographic analysis
77 transportation purposes
78 waste paper
79 water
80 yield
81 schema:name Cellulase production using natural medium and its application on enzymatic hydrolysis of thermo chemically pretreated biomass
82 schema:pagination 139
83 schema:productId N1f2ed2dd56b744208aea10b1f40d7fe9
84 Na1ebb566002945c6b640bfb5dd96029a
85 Nd916884f4fa74ac18e25856ca1171f37
86 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032088924
87 https://doi.org/10.1007/s13205-016-0465-z
88 schema:sdDatePublished 2022-10-01T06:41
89 schema:sdLicense https://scigraph.springernature.com/explorer/license/
90 schema:sdPublisher N20f62bc5e44f4f089f287f8503baa370
91 schema:url https://doi.org/10.1007/s13205-016-0465-z
92 sgo:license sg:explorer/license/
93 sgo:sdDataset articles
94 rdf:type schema:ScholarlyArticle
95 N1f2ed2dd56b744208aea10b1f40d7fe9 schema:name doi
96 schema:value 10.1007/s13205-016-0465-z
97 rdf:type schema:PropertyValue
98 N20f62bc5e44f4f089f287f8503baa370 schema:name Springer Nature - SN SciGraph project
99 rdf:type schema:Organization
100 N3602bc0321e747c1a534230f1303b990 schema:volumeNumber 6
101 rdf:type schema:PublicationVolume
102 N4f1a3f8c35c648f99ab542691a437d0f schema:issueNumber 2
103 rdf:type schema:PublicationIssue
104 N727819d571fd4ed99ea5cc85e0cf68c9 rdf:first sg:person.010375433167.50
105 rdf:rest Nf3990175a7fc470a8d16b5bbf9c136af
106 Na1ebb566002945c6b640bfb5dd96029a schema:name dimensions_id
107 schema:value pub.1032088924
108 rdf:type schema:PropertyValue
109 Naf0e3a15bbf1493297c2eb609e3a7bc4 rdf:first sg:person.012565754567.67
110 rdf:rest rdf:nil
111 Nd916884f4fa74ac18e25856ca1171f37 schema:name pubmed_id
112 schema:value 28330211
113 rdf:type schema:PropertyValue
114 Nf3990175a7fc470a8d16b5bbf9c136af rdf:first sg:person.01010037420.16
115 rdf:rest Naf0e3a15bbf1493297c2eb609e3a7bc4
116 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
117 schema:name Biological Sciences
118 rdf:type schema:DefinedTerm
119 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
120 schema:name Biochemistry and Cell Biology
121 rdf:type schema:DefinedTerm
122 sg:journal.1045498 schema:issn 2190-572X
123 2190-5738
124 schema:name 3 Biotech
125 schema:publisher Springer Nature
126 rdf:type schema:Periodical
127 sg:person.01010037420.16 schema:affiliation grid-institutes:grid.440551.1
128 schema:familyName Sharma
129 schema:givenName Vinay
130 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010037420.16
131 rdf:type schema:Person
132 sg:person.010375433167.50 schema:affiliation grid-institutes:grid.440551.1
133 schema:familyName Sharma
134 schema:givenName Shivani
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010375433167.50
136 rdf:type schema:Person
137 sg:person.012565754567.67 schema:affiliation grid-institutes:grid.440551.1
138 schema:familyName Kuila
139 schema:givenName Arindam
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012565754567.67
141 rdf:type schema:Person
142 sg:pub.10.1007/s10068-011-0178-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045267153
143 https://doi.org/10.1007/s10068-011-0178-3
144 rdf:type schema:CreativeWork
145 sg:pub.10.1186/1754-6834-6-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001199959
146 https://doi.org/10.1186/1754-6834-6-1
147 rdf:type schema:CreativeWork
148 sg:pub.10.1186/1754-6834-6-170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013521029
149 https://doi.org/10.1186/1754-6834-6-170
150 rdf:type schema:CreativeWork
151 sg:pub.10.1186/1754-6834-6-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039804556
152 https://doi.org/10.1186/1754-6834-6-8
153 rdf:type schema:CreativeWork
154 sg:pub.10.1186/2193-1801-3-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020248080
155 https://doi.org/10.1186/2193-1801-3-1
156 rdf:type schema:CreativeWork
157 sg:pub.10.1186/2193-1801-3-92 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023090777
158 https://doi.org/10.1186/2193-1801-3-92
159 rdf:type schema:CreativeWork
160 sg:pub.10.1186/s13068-014-0179-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035594727
161 https://doi.org/10.1186/s13068-014-0179-6
162 rdf:type schema:CreativeWork
163 sg:pub.10.1186/s13068-015-0419-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030058694
164 https://doi.org/10.1186/s13068-015-0419-4
165 rdf:type schema:CreativeWork
166 grid-institutes:grid.440551.1 schema:alternateName Bioscience and Biotechnology Department, Banasthali University, 304022, Banasthali, Rajasthan, India
167 schema:name Bioscience and Biotechnology Department, Banasthali University, 304022, Banasthali, Rajasthan, India
168 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...