Transformation of the symbiotic alga Oophila amblystomatis: a new tool for animal-algae symbiosis studies View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2022-06

AUTHORS

Baptiste Genot, John A. Burns

ABSTRACT

The ability to conduct reverse genetic studies in symbiotic systems is enabled by transgene expression and transformation of at least one partner. The symbiotic relationship between the yellow spotted salamander, Ambystoma maculatum, and the green alga, Oophila amblystomatis, is a unique model of vertebrate-algae symbiosis. Despite over 130 years of scientific study, there are still many open questions in this symbiosis. Transgene expression in one partner will accelerate research into the symbiotic relationship. In this paper we describe a tool and method for expression of foreign DNA in, and presumed transformation of, the alga O. amblystomatis. We successfully introduced heritable antibiotic resistance to algal cultures, and observed expression of a green fluorescent reporter protein in all transfected and presumably transformed algal populations. The outcomes of this work enable genetic manipulation of the symbiotic alga O. amblystomatis, allowing direct testing of hypotheses derived from gene expression or genomic studies that will usher in a deeper understanding of the A. maculatum-O. amblystomatis symbiotic system. More... »

PAGES

143-151

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13199-022-00861-0

DOI

http://dx.doi.org/10.1007/s13199-022-00861-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1149994623


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