Free amino acids profile of Polish and Slovak honeys based on LC–MS/MS method without the prior derivatisation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-09-07

AUTHORS

Stanisław Kowalski, Mária Kopuncová, Zuzana Ciesarová, Kristína Kukurová

ABSTRACT

LC–MS/MS method was applied for determination of free amino acids in honey without derivatisation steps. Twenty free amino acids including aspartic acid, asparagine, glutamic acid, glutamine, alanine, arginine, glycine, leucine, histidine, hydroxyproline, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, valine and ornithine were analyzed in thirty honey samples from Poland and Slovakia. The analysis covered: acacia, lime, rape, multifloral and forest types of honey. Applied method was characterized to had good sensitivity with limit of detection ranged from 3.0 ng/cm3 for valine to 13.0 ng/cm3 for hydroxyproline. Average content of proline (main amino acid component in honey) ranged from 151.46 μg/g (rape honey from Slovakia) to 389.66 μg/g for forest honey (honeydew honey) from Poland. In analyzed honeys large quantities of glutamine, glutamic acid, lysine, phenylalanine, asparagine, alanine, and valine were also found. More... »

PAGES

3716-3723

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s13197-017-2838-7

DOI

http://dx.doi.org/10.1007/s13197-017-2838-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1091500641

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29051667


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