Methylglyoxal-Induced Protection Response and Toxicity: Role of Glutathione Reductase and Thioredoxin Systems View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-05-06

AUTHORS

Ariana Ern Schmitz, Luiz Felipe de Souza, Barbara dos Santos, Pamela Maher, Fernanda Martins Lopes, Giovana Ferreira Londero, Fabio Klamt, Alcir Luiz Dafre

ABSTRACT

Thioredoxin (Trx) and glyoxalase (Glo) systems have been suggested to be molecular targets of methylglyoxal (MGO). This highly reactive endogenous compound has been associated with the development of neurodegenerative pathologies and cell death. In the present study, the glutathione (GSH), Trx, and Glo systems were investigated to understand early events (0.5–3 h) that may determine cell fate. It is shown for the first time that MGO treatment induces an increase in glutathione reductase (GR) protein in hippocampal slices (1 h) and HT22 nerve cells (0.5 and 2.5 h). Thioredoxin interacting protein (Txnip), thioredoxin reductase (TrxR), Glo1, and Glo2 were markedly increased (2- to 4-fold) in hippocampal slices and 1.2- to 1.3-fold in HT22 cells. This increase in protein levels in hippocampal slices was followed by a corresponding increase in GR, TrxR, and Glo1 activities, but not in HT22 cells. In these cells, GR and TrxR activities were decreased by MGO. This result is in agreement with the idea that MGO can affect the Trx/TrxR reducing system, and now we show that GR and Txnip can also be affected by MGO. Impairment in the GR or TrxR reducing capacity can impair peroxide removal by glutathione peroxidase and peroxiredoxin, as both peroxidases depend on reduced GSH and Trx, respectively. In this regard, inhibition of GR and TrxR by 2-AAPA or auranofin, respectively, potentiated MGO toxicity in differentiated SH-SY5Y cells. Overall, MGO not only triggers a clear defense response in hippocampal slices and HT22 cells but also impairs the Trx/TrxR and GSH/GR reducing couples in HT22 cells. The increased MGO toxicity caused by inhibition of GR and TrxR with specific inhibitors, or their inhibition by MGO treatment, supports the notion that both reducing systems are relevant molecular targets of MGO. More... »

PAGES

340-350

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12640-017-9738-5

DOI

http://dx.doi.org/10.1007/s12640-017-9738-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1085198250

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28478530


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dose-Response Relationship, Drug", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glutathione", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glutathione Reductase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hippocampus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neurons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neuroprotection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pyruvaldehyde", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thioredoxin-Disulfide Reductase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thioredoxins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tissue Culture Techniques", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biochemistry, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.411237.2", 
          "name": [
            "Department of Biochemistry, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schmitz", 
        "givenName": "Ariana Ern", 
        "id": "sg:person.01364145374.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01364145374.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biochemistry, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.411237.2", 
          "name": [
            "Department of Biochemistry, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Souza", 
        "givenName": "Luiz Felipe", 
        "id": "sg:person.0610466367.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610466367.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biochemistry, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.411237.2", 
          "name": [
            "Department of Biochemistry, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "dos Santos", 
        "givenName": "Barbara", 
        "id": "sg:person.07753475153.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07753475153.89"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cellular Neurobiology Laboratory, Salk Institute for Biological Studies, 92037, La Jolla, CA, USA", 
          "id": "http://www.grid.ac/institutes/grid.250671.7", 
          "name": [
            "Cellular Neurobiology Laboratory, Salk Institute for Biological Studies, 92037, La Jolla, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Maher", 
        "givenName": "Pamela", 
        "id": "sg:person.01033612564.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033612564.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Cellular Biochemistry, Post-Graduate Program in Biochemistry, Biochemistry Department, ICBS/UFRGS, 90035-003, Porto Alegre, Brazil", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Laboratory of Cellular Biochemistry, Post-Graduate Program in Biochemistry, Biochemistry Department, ICBS/UFRGS, 90035-003, Porto Alegre, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lopes", 
        "givenName": "Fernanda Martins", 
        "id": "sg:person.01330316535.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330316535.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Cellular Biochemistry, Post-Graduate Program in Biochemistry, Biochemistry Department, ICBS/UFRGS, 90035-003, Porto Alegre, Brazil", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Laboratory of Cellular Biochemistry, Post-Graduate Program in Biochemistry, Biochemistry Department, ICBS/UFRGS, 90035-003, Porto Alegre, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Londero", 
        "givenName": "Giovana Ferreira", 
        "id": "sg:person.0604712435.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604712435.90"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Cellular Biochemistry, Post-Graduate Program in Biochemistry, Biochemistry Department, ICBS/UFRGS, 90035-003, Porto Alegre, Brazil", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Laboratory of Cellular Biochemistry, Post-Graduate Program in Biochemistry, Biochemistry Department, ICBS/UFRGS, 90035-003, Porto Alegre, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Klamt", 
        "givenName": "Fabio", 
        "id": "sg:person.01237475752.68", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237475752.68"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biochemistry, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.411237.2", 
          "name": [
            "Department of Biochemistry, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dafre", 
        "givenName": "Alcir Luiz", 
        "id": "sg:person.01132774630.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132774630.03"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s12640-016-9699-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083410909", 
          "https://doi.org/10.1007/s12640-016-9699-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms8014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000989237", 
          "https://doi.org/10.1038/ncomms8014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/mp.2015.64", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048979493", 
          "https://doi.org/10.1038/mp.2015.64"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10719-016-9705-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021207648", 
          "https://doi.org/10.1007/s10719-016-9705-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11064-007-9402-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005747125", 
          "https://doi.org/10.1007/s11064-007-9402-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1756-0500-7-367", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007825549", 
          "https://doi.org/10.1186/1756-0500-7-367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12020-012-9795-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038477620", 
          "https://doi.org/10.1007/s12020-012-9795-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1477-5956-12-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021165248", 
          "https://doi.org/10.1186/1477-5956-12-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12964-015-0118-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052937633", 
          "https://doi.org/10.1186/s12964-015-0118-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11064-014-1481-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037598671", 
          "https://doi.org/10.1007/s11064-014-1481-1"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-05-06", 
    "datePublishedReg": "2017-05-06", 
    "description": "Thioredoxin (Trx) and glyoxalase (Glo) systems have been suggested to be molecular targets of methylglyoxal (MGO). This highly reactive endogenous compound has been associated with the development of neurodegenerative pathologies and cell death. In the present study, the glutathione (GSH), Trx, and Glo systems were investigated to understand early events (0.5\u20133\u00a0h) that may determine cell fate. It is shown for the first time that MGO treatment induces an increase in glutathione reductase (GR) protein in hippocampal slices (1\u00a0h) and HT22 nerve cells (0.5 and 2.5\u00a0h). Thioredoxin interacting protein (Txnip), thioredoxin reductase (TrxR), Glo1, and Glo2 were markedly increased (2- to 4-fold) in hippocampal slices and 1.2- to 1.3-fold in HT22 cells. This increase in protein levels in hippocampal slices was followed by a corresponding increase in GR, TrxR, and Glo1 activities, but not in HT22 cells. In these cells, GR and TrxR activities were decreased by MGO. This result is in agreement with the idea that MGO can affect the Trx/TrxR reducing system, and now we show that GR and Txnip can also be affected by MGO. Impairment in the GR or TrxR reducing capacity can impair peroxide removal by glutathione peroxidase and peroxiredoxin, as both peroxidases depend on reduced GSH and Trx, respectively. In this regard, inhibition of GR and TrxR by 2-AAPA or auranofin, respectively, potentiated MGO toxicity in differentiated SH-SY5Y cells. Overall, MGO not only triggers a clear defense response in hippocampal slices and HT22 cells but also impairs the Trx/TrxR and GSH/GR reducing couples in HT22 cells. The increased MGO toxicity caused by inhibition of GR and TrxR with specific inhibitors, or their inhibition by MGO treatment, supports the notion that both reducing systems are relevant molecular targets of MGO.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s12640-017-9738-5", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1022448", 
        "issn": [
          "1029-8428", 
          "1476-3524"
        ], 
        "name": "Neurotoxicity Research", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "32"
      }
    ], 
    "keywords": [
      "hippocampal slices", 
      "HT22 cells", 
      "inhibition of GR", 
      "MGO toxicity", 
      "MGO treatment", 
      "molecular targets", 
      "differentiated SH-SY5Y cells", 
      "Trx/TrxR", 
      "SH-SY5Y cells", 
      "relevant molecular targets", 
      "GLO1 activity", 
      "neurodegenerative pathologies", 
      "nerve cells", 
      "glutathione peroxidase", 
      "protein levels", 
      "reduced GSH", 
      "early events", 
      "cell death", 
      "specific inhibitor", 
      "endogenous compounds", 
      "inhibition", 
      "present study", 
      "toxicity", 
      "cells", 
      "treatment", 
      "glutathione reductase", 
      "slices", 
      "TrxR activity", 
      "methylglyoxal", 
      "glyoxalase system", 
      "pathology", 
      "GLO system", 
      "impairment", 
      "response", 
      "corresponding increase", 
      "death", 
      "increase", 
      "target", 
      "GR", 
      "peroxidase", 
      "TXNIP", 
      "auranofin", 
      "activity", 
      "inhibitors", 
      "protein", 
      "TrxR", 
      "protection response", 
      "Glo2", 
      "cell fate", 
      "GLO1", 
      "glutathione", 
      "thioredoxin reductase", 
      "GSH", 
      "reductase", 
      "Trx", 
      "levels", 
      "glutathione reductase protein", 
      "thioredoxin system", 
      "study", 
      "role", 
      "thioredoxin", 
      "events", 
      "first time", 
      "defense responses", 
      "regard", 
      "reductase protein", 
      "development", 
      "removal", 
      "peroxiredoxins", 
      "time", 
      "couples", 
      "results", 
      "system", 
      "capacity", 
      "compounds", 
      "fate", 
      "agreement", 
      "notion", 
      "idea", 
      "reactive endogenous compound", 
      "HT22 nerve cells", 
      "clear defense response", 
      "GSH/GR", 
      "Methylglyoxal-Induced Protection Response"
    ], 
    "name": "Methylglyoxal-Induced Protection Response and Toxicity: Role of Glutathione Reductase and Thioredoxin Systems", 
    "pagination": "340-350", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1085198250"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s12640-017-9738-5"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28478530"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s12640-017-9738-5", 
      "https://app.dimensions.ai/details/publication/pub.1085198250"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:43", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_725.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s12640-017-9738-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12640-017-9738-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12640-017-9738-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12640-017-9738-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12640-017-9738-5'


 

This table displays all metadata directly associated to this object as RDF triples.

305 TRIPLES      22 PREDICATES      136 URIs      118 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s12640-017-9738-5 schema:about N015142cba7eb4be780bfea67e2a09276
2 N03963e4a048941b585f907effde2fc69
3 N0b8487f4564449bab4186c7ee615d172
4 N10cd00cdba4a4368980685366b73e935
5 N15323dde2cad4672b976afe16f67e664
6 N3fd8f301708d4d8bb220150bb674ba6e
7 N448c8c7698e340ada5c57e69b027ed02
8 N595d0a4b42744806aa9fc142f4e7d978
9 N5beacf582c77458eae3bc80ee38f48bf
10 N5f594d6a97d641b49512a2febcd00e72
11 N6d1f8c59b232450493c7dc20433b4e0c
12 N829c9a9fb61943f4a325735d25550b6d
13 N966286cae360445e978d2f3b5c0e675c
14 Na4d25af3202745489e93d4309bec58da
15 Ne71a6c168e4645afbf430dbade2e1338
16 Nef25bd8166004156a491034186a8f0c3
17 anzsrc-for:06
18 anzsrc-for:0601
19 schema:author N35d7233a22644588bbc203ce692efbc5
20 schema:citation sg:pub.10.1007/s10719-016-9705-z
21 sg:pub.10.1007/s11064-007-9402-1
22 sg:pub.10.1007/s11064-014-1481-1
23 sg:pub.10.1007/s12020-012-9795-8
24 sg:pub.10.1007/s12640-016-9699-0
25 sg:pub.10.1038/mp.2015.64
26 sg:pub.10.1038/ncomms8014
27 sg:pub.10.1186/1477-5956-12-2
28 sg:pub.10.1186/1756-0500-7-367
29 sg:pub.10.1186/s12964-015-0118-6
30 schema:datePublished 2017-05-06
31 schema:datePublishedReg 2017-05-06
32 schema:description Thioredoxin (Trx) and glyoxalase (Glo) systems have been suggested to be molecular targets of methylglyoxal (MGO). This highly reactive endogenous compound has been associated with the development of neurodegenerative pathologies and cell death. In the present study, the glutathione (GSH), Trx, and Glo systems were investigated to understand early events (0.5–3 h) that may determine cell fate. It is shown for the first time that MGO treatment induces an increase in glutathione reductase (GR) protein in hippocampal slices (1 h) and HT22 nerve cells (0.5 and 2.5 h). Thioredoxin interacting protein (Txnip), thioredoxin reductase (TrxR), Glo1, and Glo2 were markedly increased (2- to 4-fold) in hippocampal slices and 1.2- to 1.3-fold in HT22 cells. This increase in protein levels in hippocampal slices was followed by a corresponding increase in GR, TrxR, and Glo1 activities, but not in HT22 cells. In these cells, GR and TrxR activities were decreased by MGO. This result is in agreement with the idea that MGO can affect the Trx/TrxR reducing system, and now we show that GR and Txnip can also be affected by MGO. Impairment in the GR or TrxR reducing capacity can impair peroxide removal by glutathione peroxidase and peroxiredoxin, as both peroxidases depend on reduced GSH and Trx, respectively. In this regard, inhibition of GR and TrxR by 2-AAPA or auranofin, respectively, potentiated MGO toxicity in differentiated SH-SY5Y cells. Overall, MGO not only triggers a clear defense response in hippocampal slices and HT22 cells but also impairs the Trx/TrxR and GSH/GR reducing couples in HT22 cells. The increased MGO toxicity caused by inhibition of GR and TrxR with specific inhibitors, or their inhibition by MGO treatment, supports the notion that both reducing systems are relevant molecular targets of MGO.
33 schema:genre article
34 schema:inLanguage en
35 schema:isAccessibleForFree false
36 schema:isPartOf N05cf2e5b735c447cb39490c6f3eb462d
37 Nf78d667683fc492692bcc3b94a324a53
38 sg:journal.1022448
39 schema:keywords GLO system
40 GLO1
41 GLO1 activity
42 GR
43 GSH
44 GSH/GR
45 Glo2
46 HT22 cells
47 HT22 nerve cells
48 MGO toxicity
49 MGO treatment
50 Methylglyoxal-Induced Protection Response
51 SH-SY5Y cells
52 TXNIP
53 Trx
54 Trx/TrxR
55 TrxR
56 TrxR activity
57 activity
58 agreement
59 auranofin
60 capacity
61 cell death
62 cell fate
63 cells
64 clear defense response
65 compounds
66 corresponding increase
67 couples
68 death
69 defense responses
70 development
71 differentiated SH-SY5Y cells
72 early events
73 endogenous compounds
74 events
75 fate
76 first time
77 glutathione
78 glutathione peroxidase
79 glutathione reductase
80 glutathione reductase protein
81 glyoxalase system
82 hippocampal slices
83 idea
84 impairment
85 increase
86 inhibition
87 inhibition of GR
88 inhibitors
89 levels
90 methylglyoxal
91 molecular targets
92 nerve cells
93 neurodegenerative pathologies
94 notion
95 pathology
96 peroxidase
97 peroxiredoxins
98 present study
99 protection response
100 protein
101 protein levels
102 reactive endogenous compound
103 reduced GSH
104 reductase
105 reductase protein
106 regard
107 relevant molecular targets
108 removal
109 response
110 results
111 role
112 slices
113 specific inhibitor
114 study
115 system
116 target
117 thioredoxin
118 thioredoxin reductase
119 thioredoxin system
120 time
121 toxicity
122 treatment
123 schema:name Methylglyoxal-Induced Protection Response and Toxicity: Role of Glutathione Reductase and Thioredoxin Systems
124 schema:pagination 340-350
125 schema:productId N3a9b98090cbe4441b88b7bab76825f1f
126 N49b1299e8748430d85271f891d3dca5a
127 Nfb128632118f4a21aa917a7bd31e3370
128 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085198250
129 https://doi.org/10.1007/s12640-017-9738-5
130 schema:sdDatePublished 2022-01-01T18:43
131 schema:sdLicense https://scigraph.springernature.com/explorer/license/
132 schema:sdPublisher N8c1eddbbe08f4be1b58906fdf98d985d
133 schema:url https://doi.org/10.1007/s12640-017-9738-5
134 sgo:license sg:explorer/license/
135 sgo:sdDataset articles
136 rdf:type schema:ScholarlyArticle
137 N015142cba7eb4be780bfea67e2a09276 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Glutathione
139 rdf:type schema:DefinedTerm
140 N03963e4a048941b585f907effde2fc69 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Tissue Culture Techniques
142 rdf:type schema:DefinedTerm
143 N05cf2e5b735c447cb39490c6f3eb462d schema:issueNumber 3
144 rdf:type schema:PublicationIssue
145 N0b8487f4564449bab4186c7ee615d172 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Dose-Response Relationship, Drug
147 rdf:type schema:DefinedTerm
148 N10cd00cdba4a4368980685366b73e935 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Thioredoxin-Disulfide Reductase
150 rdf:type schema:DefinedTerm
151 N15323dde2cad4672b976afe16f67e664 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Neurons
153 rdf:type schema:DefinedTerm
154 N27e250fa4df544409100398098171609 rdf:first sg:person.0604712435.90
155 rdf:rest Nffc1cf9b196748d4ad6b1f3e2b5bee3a
156 N35d7233a22644588bbc203ce692efbc5 rdf:first sg:person.01364145374.03
157 rdf:rest Nfdcd0b452a7f4b1b92c47df3e206826e
158 N3a9b98090cbe4441b88b7bab76825f1f schema:name pubmed_id
159 schema:value 28478530
160 rdf:type schema:PropertyValue
161 N3fd8f301708d4d8bb220150bb674ba6e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Glutathione Reductase
163 rdf:type schema:DefinedTerm
164 N448c8c7698e340ada5c57e69b027ed02 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Mice
166 rdf:type schema:DefinedTerm
167 N49b1299e8748430d85271f891d3dca5a schema:name dimensions_id
168 schema:value pub.1085198250
169 rdf:type schema:PropertyValue
170 N595d0a4b42744806aa9fc142f4e7d978 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Animals
172 rdf:type schema:DefinedTerm
173 N5beacf582c77458eae3bc80ee38f48bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
174 schema:name Cell Line
175 rdf:type schema:DefinedTerm
176 N5f594d6a97d641b49512a2febcd00e72 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Pyruvaldehyde
178 rdf:type schema:DefinedTerm
179 N6d1f8c59b232450493c7dc20433b4e0c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Neuroprotection
181 rdf:type schema:DefinedTerm
182 N829c9a9fb61943f4a325735d25550b6d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
183 schema:name Hippocampus
184 rdf:type schema:DefinedTerm
185 N8c1eddbbe08f4be1b58906fdf98d985d schema:name Springer Nature - SN SciGraph project
186 rdf:type schema:Organization
187 N966286cae360445e978d2f3b5c0e675c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name Humans
189 rdf:type schema:DefinedTerm
190 Na4d25af3202745489e93d4309bec58da schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name Female
192 rdf:type schema:DefinedTerm
193 Nbd48b38d42c0442bbdcd98d6c3300472 rdf:first sg:person.07753475153.89
194 rdf:rest Nf1dfbb17c1b0417792ed777a4cfb25b2
195 Nbf42474e4f5240fcba57e07fcd7cf384 rdf:first sg:person.01330316535.78
196 rdf:rest N27e250fa4df544409100398098171609
197 Ncb0d354d09bb416da8f22ac270322757 rdf:first sg:person.01132774630.03
198 rdf:rest rdf:nil
199 Ne71a6c168e4645afbf430dbade2e1338 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
200 schema:name Cell Survival
201 rdf:type schema:DefinedTerm
202 Nef25bd8166004156a491034186a8f0c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
203 schema:name Thioredoxins
204 rdf:type schema:DefinedTerm
205 Nf1dfbb17c1b0417792ed777a4cfb25b2 rdf:first sg:person.01033612564.03
206 rdf:rest Nbf42474e4f5240fcba57e07fcd7cf384
207 Nf78d667683fc492692bcc3b94a324a53 schema:volumeNumber 32
208 rdf:type schema:PublicationVolume
209 Nfb128632118f4a21aa917a7bd31e3370 schema:name doi
210 schema:value 10.1007/s12640-017-9738-5
211 rdf:type schema:PropertyValue
212 Nfdcd0b452a7f4b1b92c47df3e206826e rdf:first sg:person.0610466367.78
213 rdf:rest Nbd48b38d42c0442bbdcd98d6c3300472
214 Nffc1cf9b196748d4ad6b1f3e2b5bee3a rdf:first sg:person.01237475752.68
215 rdf:rest Ncb0d354d09bb416da8f22ac270322757
216 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
217 schema:name Biological Sciences
218 rdf:type schema:DefinedTerm
219 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
220 schema:name Biochemistry and Cell Biology
221 rdf:type schema:DefinedTerm
222 sg:journal.1022448 schema:issn 1029-8428
223 1476-3524
224 schema:name Neurotoxicity Research
225 schema:publisher Springer Nature
226 rdf:type schema:Periodical
227 sg:person.01033612564.03 schema:affiliation grid-institutes:grid.250671.7
228 schema:familyName Maher
229 schema:givenName Pamela
230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033612564.03
231 rdf:type schema:Person
232 sg:person.01132774630.03 schema:affiliation grid-institutes:grid.411237.2
233 schema:familyName Dafre
234 schema:givenName Alcir Luiz
235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132774630.03
236 rdf:type schema:Person
237 sg:person.01237475752.68 schema:affiliation grid-institutes:None
238 schema:familyName Klamt
239 schema:givenName Fabio
240 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237475752.68
241 rdf:type schema:Person
242 sg:person.01330316535.78 schema:affiliation grid-institutes:None
243 schema:familyName Lopes
244 schema:givenName Fernanda Martins
245 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330316535.78
246 rdf:type schema:Person
247 sg:person.01364145374.03 schema:affiliation grid-institutes:grid.411237.2
248 schema:familyName Schmitz
249 schema:givenName Ariana Ern
250 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01364145374.03
251 rdf:type schema:Person
252 sg:person.0604712435.90 schema:affiliation grid-institutes:None
253 schema:familyName Londero
254 schema:givenName Giovana Ferreira
255 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604712435.90
256 rdf:type schema:Person
257 sg:person.0610466367.78 schema:affiliation grid-institutes:grid.411237.2
258 schema:familyName de Souza
259 schema:givenName Luiz Felipe
260 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610466367.78
261 rdf:type schema:Person
262 sg:person.07753475153.89 schema:affiliation grid-institutes:grid.411237.2
263 schema:familyName dos Santos
264 schema:givenName Barbara
265 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07753475153.89
266 rdf:type schema:Person
267 sg:pub.10.1007/s10719-016-9705-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1021207648
268 https://doi.org/10.1007/s10719-016-9705-z
269 rdf:type schema:CreativeWork
270 sg:pub.10.1007/s11064-007-9402-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005747125
271 https://doi.org/10.1007/s11064-007-9402-1
272 rdf:type schema:CreativeWork
273 sg:pub.10.1007/s11064-014-1481-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037598671
274 https://doi.org/10.1007/s11064-014-1481-1
275 rdf:type schema:CreativeWork
276 sg:pub.10.1007/s12020-012-9795-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038477620
277 https://doi.org/10.1007/s12020-012-9795-8
278 rdf:type schema:CreativeWork
279 sg:pub.10.1007/s12640-016-9699-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083410909
280 https://doi.org/10.1007/s12640-016-9699-0
281 rdf:type schema:CreativeWork
282 sg:pub.10.1038/mp.2015.64 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048979493
283 https://doi.org/10.1038/mp.2015.64
284 rdf:type schema:CreativeWork
285 sg:pub.10.1038/ncomms8014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000989237
286 https://doi.org/10.1038/ncomms8014
287 rdf:type schema:CreativeWork
288 sg:pub.10.1186/1477-5956-12-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021165248
289 https://doi.org/10.1186/1477-5956-12-2
290 rdf:type schema:CreativeWork
291 sg:pub.10.1186/1756-0500-7-367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007825549
292 https://doi.org/10.1186/1756-0500-7-367
293 rdf:type schema:CreativeWork
294 sg:pub.10.1186/s12964-015-0118-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052937633
295 https://doi.org/10.1186/s12964-015-0118-6
296 rdf:type schema:CreativeWork
297 grid-institutes:None schema:alternateName Laboratory of Cellular Biochemistry, Post-Graduate Program in Biochemistry, Biochemistry Department, ICBS/UFRGS, 90035-003, Porto Alegre, Brazil
298 schema:name Laboratory of Cellular Biochemistry, Post-Graduate Program in Biochemistry, Biochemistry Department, ICBS/UFRGS, 90035-003, Porto Alegre, Brazil
299 rdf:type schema:Organization
300 grid-institutes:grid.250671.7 schema:alternateName Cellular Neurobiology Laboratory, Salk Institute for Biological Studies, 92037, La Jolla, CA, USA
301 schema:name Cellular Neurobiology Laboratory, Salk Institute for Biological Studies, 92037, La Jolla, CA, USA
302 rdf:type schema:Organization
303 grid-institutes:grid.411237.2 schema:alternateName Department of Biochemistry, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil
304 schema:name Department of Biochemistry, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil
305 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...