Electrophysiology, immunophenotype, and gene expression characterization of senescent and cryopreserved human amniotic fluid stem cells View Full Text


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Article Info

DATE

2016-11

AUTHORS

Florin Iordache, Andrei Constantinescu, Eugen Andrei, Bogdan Amuzescu, Ferdinand Halitzchi, Lorand Savu, Horia Maniu

ABSTRACT

We characterized human amniotic fluid stem cells (AFSC) in senescent cultures (6 weeks) versus cryopreserved cells using whole-cell patch-clamp, immunophenotyping, and differential gene expression profiling for senescence genes. We evidenced five ion current components (outward rectifier, A-type, inward rectifier, and big conductance Ca2+-dependent K+ currents, fast voltage-dependent Na+ currents). Senescent AFSC showed reduced expression of CD90, CD44, CD133, over 500-fold increase of interferon gamma and telomerase reverse transcriptase genes, increased cycle-dependent kinase 4 inhibitors, p53-binding protein 1, and decreased calreticulin and CD44. HLA-ABC immune expression was similar, and HLA-DR expression very low in both cell types. A subset of cryopreserved AFSC featured large inward rectifier K+ currents, voltage-dependent Na+ currents, and neural progenitor markers evidenced by immunophenotyping and RT-PCR. In all AFSC, in both culture conditions, at patch rupture the outward currents were very low, and they increased progressively over several minutes upon cytoplasm dialysis with pipette solution. More... »

PAGES

463-476

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s12576-016-0441-8

    DOI

    http://dx.doi.org/10.1007/s12576-016-0441-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1018533733

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27053101


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    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12576-016-0441-8'

    Turtle is a human-readable linked data format.

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    RDF/XML is a standard XML format for linked data.

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