Electrophysiology, immunophenotype, and gene expression characterization of senescent and cryopreserved human amniotic fluid stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-11

AUTHORS

Florin Iordache, Andrei Constantinescu, Eugen Andrei, Bogdan Amuzescu, Ferdinand Halitzchi, Lorand Savu, Horia Maniu

ABSTRACT

We characterized human amniotic fluid stem cells (AFSC) in senescent cultures (6 weeks) versus cryopreserved cells using whole-cell patch-clamp, immunophenotyping, and differential gene expression profiling for senescence genes. We evidenced five ion current components (outward rectifier, A-type, inward rectifier, and big conductance Ca2+-dependent K+ currents, fast voltage-dependent Na+ currents). Senescent AFSC showed reduced expression of CD90, CD44, CD133, over 500-fold increase of interferon gamma and telomerase reverse transcriptase genes, increased cycle-dependent kinase 4 inhibitors, p53-binding protein 1, and decreased calreticulin and CD44. HLA-ABC immune expression was similar, and HLA-DR expression very low in both cell types. A subset of cryopreserved AFSC featured large inward rectifier K+ currents, voltage-dependent Na+ currents, and neural progenitor markers evidenced by immunophenotyping and RT-PCR. In all AFSC, in both culture conditions, at patch rupture the outward currents were very low, and they increased progressively over several minutes upon cytoplasm dialysis with pipette solution. More... »

PAGES

463-476

References to SciGraph publications

  • 2012-10. Human amniotic fluid-derived c-kit+ and c-kit− stem cells: growth characteristics and some differentiation potential capacities comparison in CYTOTECHNOLOGY
  • 2004-12. Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4 in BMC NEUROSCIENCE
  • 2012-08. Potential antitumor therapeutic strategies of human amniotic membrane and amniotic fluid-derived stem cells in CANCER GENE THERAPY
  • 2014-07. Human amniotic fluid stem cells: neural differentiation in vitro and in vivo in CELL AND TISSUE RESEARCH
  • 2007-01. Isolation of amniotic stem cell lines with potential for therapy in NATURE BIOTECHNOLOGY
  • 2013-04. AF-MSCs fate can be regulated by culture conditions in CELL DEATH & DISEASE
  • 2000-09. Tissue-engineered bone regeneration in NATURE BIOTECHNOLOGY
  • 2014-06. Human placenta-derived neurospheres are susceptible to transformation after extensive in vitro expansion in STEM CELL RESEARCH & THERAPY
  • 2009-11. Adenovirus-mediated hypoxia-inducible factor 1α double-mutant promotes differentiation of bone marrow stem cells to cardiomyocytes in THE JOURNAL OF PHYSIOLOGICAL SCIENCES
  • 2016-05. Neural stem cells and neuro/gliogenesis in the central nervous system: understanding the structural and functional plasticity of the developing, mature, and diseased brain in THE JOURNAL OF PHYSIOLOGICAL SCIENCES
  • 2000-02. Modulation of A-type potassium channels by a family of calcium sensors in NATURE
  • 2010-03. Electrophysiological and contractile evidence of the ability of human mesenchymal stromal cells to correct vascular malfunction in rats after ionizing irradiation in THE JOURNAL OF PHYSIOLOGICAL SCIENCES
  • 2014-08. Amniotic fluid-derived mesenchymal stem cells: characteristics and therapeutic applications in ARCHIVES OF GYNECOLOGY AND OBSTETRICS
  • 2010-09. The heterogeneity of cell subtypes from a primary culture of human amniotic fluid in CELLULAR & MOLECULAR BIOLOGY LETTERS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s12576-016-0441-8

    DOI

    http://dx.doi.org/10.1007/s12576-016-0441-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1018533733

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27053101


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amniotic Fluid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Culture Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cryopreservation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Electrophysiological Phenomena", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Immunophenotyping", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stem Cells", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "name": [
                "Flow Cytometry and Cell Therapy Laboratory, \u201cN. Simionescu\u201d Institute of Cellular Biology and Pathology, B.P. Hasdeu Str. 8, 050568, Bucharest, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Iordache", 
            "givenName": "Florin", 
            "id": "sg:person.01154217362.11", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154217362.11"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Flow Cytometry and Cell Therapy Laboratory, \u201cN. Simionescu\u201d Institute of Cellular Biology and Pathology, B.P. Hasdeu Str. 8, 050568, Bucharest, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Constantinescu", 
            "givenName": "Andrei", 
            "id": "sg:person.0740056540.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0740056540.19"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Flow Cytometry and Cell Therapy Laboratory, \u201cN. Simionescu\u201d Institute of Cellular Biology and Pathology, B.P. Hasdeu Str. 8, 050568, Bucharest, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Andrei", 
            "givenName": "Eugen", 
            "id": "sg:person.01006171740.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006171740.29"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Bucharest", 
              "id": "https://www.grid.ac/institutes/grid.5100.4", 
              "name": [
                "Department Biophysics and Physiology, Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, 050095, Bucharest, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Amuzescu", 
            "givenName": "Bogdan", 
            "id": "sg:person.01337534000.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337534000.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Bucharest", 
              "id": "https://www.grid.ac/institutes/grid.5100.4", 
              "name": [
                "Department Biophysics and Physiology, Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, 050095, Bucharest, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Halitzchi", 
            "givenName": "Ferdinand", 
            "id": "sg:person.01310475221.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310475221.17"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Genetic Lab SRL, Cpt. Av. N. Drossu Str. 9, 012071, Bucharest, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Savu", 
            "givenName": "Lorand", 
            "id": "sg:person.01130563524.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130563524.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Flow Cytometry and Cell Therapy Laboratory, \u201cN. Simionescu\u201d Institute of Cellular Biology and Pathology, B.P. Hasdeu Str. 8, 050568, Bucharest, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Maniu", 
            "givenName": "Horia", 
            "id": "sg:person.01054305140.91", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054305140.91"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1371/journal.pone.0053372", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006239776"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/79449", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007162451", 
              "https://doi.org/10.1038/79449"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/79449", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007162451", 
              "https://doi.org/10.1038/79449"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/stemcells.2004-0213", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007233184"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cgt.2012.30", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009806288", 
              "https://doi.org/10.1038/cgt.2012.30"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00441-014-1840-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011389023", 
              "https://doi.org/10.1007/s00441-014-1840-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00441-014-1840-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011389023", 
              "https://doi.org/10.1007/s00441-014-1840-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1152/ajpcell.00251.2009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011665653"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1113/jphysiol.1980.sp013113", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011811711"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1582-4934.2004.tb00320.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012020922"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.2478/s11658-010-0017-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012021568", 
              "https://doi.org/10.2478/s11658-010-0017-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.2478/s11658-010-0017-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012021568", 
              "https://doi.org/10.2478/s11658-010-0017-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1152/ajpcell.00326.2011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012294622"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00404-014-3231-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016583613", 
              "https://doi.org/10.1007/s00404-014-3231-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/scrt444", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021531310", 
              "https://doi.org/10.1186/scrt444"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/14653240902974024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022131095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/stemcells.2004-0149", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023081875"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2008.07.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024126337"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1274", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028121369", 
              "https://doi.org/10.1038/nbt1274"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1274", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028121369", 
              "https://doi.org/10.1038/nbt1274"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.tibtech.2015.04.003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028729000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12576-015-0421-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030208980", 
              "https://doi.org/10.1007/s12576-015-0421-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cddis.2013.93", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031106058", 
              "https://doi.org/10.1038/cddis.2013.93"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35000592", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036144012", 
              "https://doi.org/10.1038/35000592"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35000592", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036144012", 
              "https://doi.org/10.1038/35000592"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12576-009-0050-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037892926", 
              "https://doi.org/10.1007/s12576-009-0050-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12576-009-0050-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037892926", 
              "https://doi.org/10.1007/s12576-009-0050-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0073816", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038913386"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10616-012-9441-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038923544", 
              "https://doi.org/10.1007/s10616-012-9441-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12576-009-0080-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041084858", 
              "https://doi.org/10.1007/s12576-009-0080-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12576-009-0080-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041084858", 
              "https://doi.org/10.1007/s12576-009-0080-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/humrep/deh279", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042246019"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.aaa5619", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042259520"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/cyto.a.22402", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045598329"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jacc.2007.07.041", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048138798"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4061/2010/369578", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051071261"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2202-5-33", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053265151", 
              "https://doi.org/10.1186/1471-2202-5-33"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1095/biolreprod.105.046029", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060111035"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1387/ijdb.092935md", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1067191121"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2174/138920111794295710", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069177780"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/mmr.2011.499", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1071520138"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/org.22426", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072306763"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.5966/sctm.2013-0081", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1073577423"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1200/jco.2000.18.2.307", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1074577133"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2016-11", 
        "datePublishedReg": "2016-11-01", 
        "description": "We characterized human amniotic fluid stem cells (AFSC) in senescent cultures (6\u00a0weeks) versus cryopreserved cells using whole-cell patch-clamp, immunophenotyping, and differential gene expression profiling for senescence genes. We evidenced five ion current components (outward rectifier, A-type, inward rectifier, and big conductance Ca2+-dependent K+ currents, fast voltage-dependent Na+ currents). Senescent AFSC showed reduced expression of CD90, CD44, CD133, over 500-fold increase of interferon gamma and telomerase reverse transcriptase genes, increased cycle-dependent kinase 4 inhibitors, p53-binding protein 1, and decreased calreticulin and CD44. HLA-ABC immune expression was similar, and HLA-DR expression very low in both cell types. A subset of cryopreserved AFSC featured large inward rectifier K+ currents, voltage-dependent Na+ currents, and neural progenitor markers evidenced by immunophenotyping and RT-PCR. In all AFSC, in both culture conditions, at patch rupture the outward currents were very low, and they increased progressively over several minutes upon cytoplasm dialysis with pipette solution.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s12576-016-0441-8", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1294916", 
            "issn": [
              "1880-6546", 
              "1880-6562"
            ], 
            "name": "The Journal of Physiological Sciences", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "6", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "66"
          }
        ], 
        "name": "Electrophysiology, immunophenotype, and gene expression characterization of senescent and cryopreserved human amniotic fluid stem cells", 
        "pagination": "463-476", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "584e493a7e3949df65bb973f3bef1eaa1b90f0f4efb40acf19b4fa0038fad4be"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "27053101"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101262417"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s12576-016-0441-8"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1018533733"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s12576-016-0441-8", 
          "https://app.dimensions.ai/details/publication/pub.1018533733"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T14:12", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000521.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs12576-016-0441-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12576-016-0441-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12576-016-0441-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12576-016-0441-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12576-016-0441-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    282 TRIPLES      21 PREDICATES      75 URIs      30 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s12576-016-0441-8 schema:about N0ed8405247444a55a3553d8b69ae8c70
    2 N2534a2a2e9aa448da61c42ccd58f11e1
    3 N2a9add5c52084baea14083fec8de036f
    4 N3e76552c8fd1495da76e05a6fad98f89
    5 N640800ddec654e54afd9f5a98e5757b9
    6 N7ad1bb7826984556b36318ed06cb4c7b
    7 N9683ddbd8847447db60208eceb47157b
    8 Nb347eb939506439cb10cb1a91ecdf32d
    9 Nf4550b211b9e4315a3ed46be88c195dd
    10 anzsrc-for:06
    11 anzsrc-for:0601
    12 schema:author N8117ffb0cebd4a56a6ba3680eab76021
    13 schema:citation sg:pub.10.1007/s00404-014-3231-7
    14 sg:pub.10.1007/s00441-014-1840-x
    15 sg:pub.10.1007/s10616-012-9441-6
    16 sg:pub.10.1007/s12576-009-0050-x
    17 sg:pub.10.1007/s12576-009-0080-4
    18 sg:pub.10.1007/s12576-015-0421-4
    19 sg:pub.10.1038/35000592
    20 sg:pub.10.1038/79449
    21 sg:pub.10.1038/cddis.2013.93
    22 sg:pub.10.1038/cgt.2012.30
    23 sg:pub.10.1038/nbt1274
    24 sg:pub.10.1186/1471-2202-5-33
    25 sg:pub.10.1186/scrt444
    26 sg:pub.10.2478/s11658-010-0017-1
    27 https://doi.org/10.1002/cyto.a.22402
    28 https://doi.org/10.1016/j.jacc.2007.07.041
    29 https://doi.org/10.1016/j.stem.2008.07.001
    30 https://doi.org/10.1016/j.tibtech.2015.04.003
    31 https://doi.org/10.1080/14653240902974024
    32 https://doi.org/10.1093/humrep/deh279
    33 https://doi.org/10.1095/biolreprod.105.046029
    34 https://doi.org/10.1111/j.1582-4934.2004.tb00320.x
    35 https://doi.org/10.1113/jphysiol.1980.sp013113
    36 https://doi.org/10.1126/science.aaa5619
    37 https://doi.org/10.1152/ajpcell.00251.2009
    38 https://doi.org/10.1152/ajpcell.00326.2011
    39 https://doi.org/10.1200/jco.2000.18.2.307
    40 https://doi.org/10.1371/journal.pone.0053372
    41 https://doi.org/10.1371/journal.pone.0073816
    42 https://doi.org/10.1387/ijdb.092935md
    43 https://doi.org/10.1634/stemcells.2004-0149
    44 https://doi.org/10.1634/stemcells.2004-0213
    45 https://doi.org/10.2174/138920111794295710
    46 https://doi.org/10.3892/mmr.2011.499
    47 https://doi.org/10.4061/2010/369578
    48 https://doi.org/10.4161/org.22426
    49 https://doi.org/10.5966/sctm.2013-0081
    50 schema:datePublished 2016-11
    51 schema:datePublishedReg 2016-11-01
    52 schema:description We characterized human amniotic fluid stem cells (AFSC) in senescent cultures (6 weeks) versus cryopreserved cells using whole-cell patch-clamp, immunophenotyping, and differential gene expression profiling for senescence genes. We evidenced five ion current components (outward rectifier, A-type, inward rectifier, and big conductance Ca<sup>2+</sup>-dependent K<sup>+</sup> currents, fast voltage-dependent Na<sup>+</sup> currents). Senescent AFSC showed reduced expression of CD90, CD44, CD133, over 500-fold increase of interferon gamma and telomerase reverse transcriptase genes, increased cycle-dependent kinase 4 inhibitors, p53-binding protein 1, and decreased calreticulin and CD44. HLA-ABC immune expression was similar, and HLA-DR expression very low in both cell types. A subset of cryopreserved AFSC featured large inward rectifier K<sup>+</sup> currents, voltage-dependent Na<sup>+</sup> currents, and neural progenitor markers evidenced by immunophenotyping and RT-PCR. In all AFSC, in both culture conditions, at patch rupture the outward currents were very low, and they increased progressively over several minutes upon cytoplasm dialysis with pipette solution.
    53 schema:genre research_article
    54 schema:inLanguage en
    55 schema:isAccessibleForFree false
    56 schema:isPartOf N45da319d90b24fde85bb3e202a316819
    57 Nb0ac3ace45734b508c03e6273891682c
    58 sg:journal.1294916
    59 schema:name Electrophysiology, immunophenotype, and gene expression characterization of senescent and cryopreserved human amniotic fluid stem cells
    60 schema:pagination 463-476
    61 schema:productId N0b7e97d2419b43009328a316870e6ffd
    62 N11c3b710c39049df90bff2c79528cdd0
    63 Nd03aaf9ecf134cc9adce38e6edc92221
    64 Nf98f421afc734d27821d489544e9c70c
    65 Nf9c529c150ea46c48a0a4945e22d76d1
    66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018533733
    67 https://doi.org/10.1007/s12576-016-0441-8
    68 schema:sdDatePublished 2019-04-10T14:12
    69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    70 schema:sdPublisher N34095962610b4642bf468c97d70b1ff0
    71 schema:url http://link.springer.com/10.1007%2Fs12576-016-0441-8
    72 sgo:license sg:explorer/license/
    73 sgo:sdDataset articles
    74 rdf:type schema:ScholarlyArticle
    75 N0b7e97d2419b43009328a316870e6ffd schema:name dimensions_id
    76 schema:value pub.1018533733
    77 rdf:type schema:PropertyValue
    78 N0ed8405247444a55a3553d8b69ae8c70 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    79 schema:name Gene Expression Regulation
    80 rdf:type schema:DefinedTerm
    81 N11c3b710c39049df90bff2c79528cdd0 schema:name nlm_unique_id
    82 schema:value 101262417
    83 rdf:type schema:PropertyValue
    84 N11f92184870544ad8e1b4ada9d785a2c schema:name Genetic Lab SRL, Cpt. Av. N. Drossu Str. 9, 012071, Bucharest, Romania
    85 rdf:type schema:Organization
    86 N1400f228e78d434fa54ec04d43ea2891 rdf:first sg:person.01054305140.91
    87 rdf:rest rdf:nil
    88 N2534a2a2e9aa448da61c42ccd58f11e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name Amniotic Fluid
    90 rdf:type schema:DefinedTerm
    91 N2a9add5c52084baea14083fec8de036f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name Cells, Cultured
    93 rdf:type schema:DefinedTerm
    94 N34095962610b4642bf468c97d70b1ff0 schema:name Springer Nature - SN SciGraph project
    95 rdf:type schema:Organization
    96 N3e76552c8fd1495da76e05a6fad98f89 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Humans
    98 rdf:type schema:DefinedTerm
    99 N45da319d90b24fde85bb3e202a316819 schema:issueNumber 6
    100 rdf:type schema:PublicationIssue
    101 N640800ddec654e54afd9f5a98e5757b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Immunophenotyping
    103 rdf:type schema:DefinedTerm
    104 N67664bbd83994eddbb687235b7938511 rdf:first sg:person.01337534000.32
    105 rdf:rest N69711dbc48e240c3b0eb991eefd27b03
    106 N69711dbc48e240c3b0eb991eefd27b03 rdf:first sg:person.01310475221.17
    107 rdf:rest N6aa2ddbf3de74962b0cb15147819c758
    108 N6aa2ddbf3de74962b0cb15147819c758 rdf:first sg:person.01130563524.34
    109 rdf:rest N1400f228e78d434fa54ec04d43ea2891
    110 N7ad1bb7826984556b36318ed06cb4c7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Stem Cells
    112 rdf:type schema:DefinedTerm
    113 N8117ffb0cebd4a56a6ba3680eab76021 rdf:first sg:person.01154217362.11
    114 rdf:rest N965627f031ef4fb79d30946b5bedbfc0
    115 N8de5a45d581d4b5c99470412eaceed02 schema:name Flow Cytometry and Cell Therapy Laboratory, “N. Simionescu” Institute of Cellular Biology and Pathology, B.P. Hasdeu Str. 8, 050568, Bucharest, Romania
    116 rdf:type schema:Organization
    117 N93102be470e54a2e844a4110117f234d schema:name Flow Cytometry and Cell Therapy Laboratory, “N. Simionescu” Institute of Cellular Biology and Pathology, B.P. Hasdeu Str. 8, 050568, Bucharest, Romania
    118 rdf:type schema:Organization
    119 N965627f031ef4fb79d30946b5bedbfc0 rdf:first sg:person.0740056540.19
    120 rdf:rest Nd2825f14c9944f80a1b551b7398cd9e3
    121 N9683ddbd8847447db60208eceb47157b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Electrophysiological Phenomena
    123 rdf:type schema:DefinedTerm
    124 Na310f0d3c0384970897c01d467c19b8b schema:name Flow Cytometry and Cell Therapy Laboratory, “N. Simionescu” Institute of Cellular Biology and Pathology, B.P. Hasdeu Str. 8, 050568, Bucharest, Romania
    125 rdf:type schema:Organization
    126 Nb0ac3ace45734b508c03e6273891682c schema:volumeNumber 66
    127 rdf:type schema:PublicationVolume
    128 Nb347eb939506439cb10cb1a91ecdf32d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Cell Culture Techniques
    130 rdf:type schema:DefinedTerm
    131 Nd03aaf9ecf134cc9adce38e6edc92221 schema:name pubmed_id
    132 schema:value 27053101
    133 rdf:type schema:PropertyValue
    134 Nd2825f14c9944f80a1b551b7398cd9e3 rdf:first sg:person.01006171740.29
    135 rdf:rest N67664bbd83994eddbb687235b7938511
    136 Nf4550b211b9e4315a3ed46be88c195dd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Cryopreservation
    138 rdf:type schema:DefinedTerm
    139 Nf98f421afc734d27821d489544e9c70c schema:name readcube_id
    140 schema:value 584e493a7e3949df65bb973f3bef1eaa1b90f0f4efb40acf19b4fa0038fad4be
    141 rdf:type schema:PropertyValue
    142 Nf9c529c150ea46c48a0a4945e22d76d1 schema:name doi
    143 schema:value 10.1007/s12576-016-0441-8
    144 rdf:type schema:PropertyValue
    145 Nfdf2974e0f2b4342954b90ac9fbc1ea0 schema:name Flow Cytometry and Cell Therapy Laboratory, “N. Simionescu” Institute of Cellular Biology and Pathology, B.P. Hasdeu Str. 8, 050568, Bucharest, Romania
    146 rdf:type schema:Organization
    147 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    148 schema:name Biological Sciences
    149 rdf:type schema:DefinedTerm
    150 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    151 schema:name Biochemistry and Cell Biology
    152 rdf:type schema:DefinedTerm
    153 sg:journal.1294916 schema:issn 1880-6546
    154 1880-6562
    155 schema:name The Journal of Physiological Sciences
    156 rdf:type schema:Periodical
    157 sg:person.01006171740.29 schema:affiliation Nfdf2974e0f2b4342954b90ac9fbc1ea0
    158 schema:familyName Andrei
    159 schema:givenName Eugen
    160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006171740.29
    161 rdf:type schema:Person
    162 sg:person.01054305140.91 schema:affiliation Na310f0d3c0384970897c01d467c19b8b
    163 schema:familyName Maniu
    164 schema:givenName Horia
    165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054305140.91
    166 rdf:type schema:Person
    167 sg:person.01130563524.34 schema:affiliation N11f92184870544ad8e1b4ada9d785a2c
    168 schema:familyName Savu
    169 schema:givenName Lorand
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130563524.34
    171 rdf:type schema:Person
    172 sg:person.01154217362.11 schema:affiliation N8de5a45d581d4b5c99470412eaceed02
    173 schema:familyName Iordache
    174 schema:givenName Florin
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154217362.11
    176 rdf:type schema:Person
    177 sg:person.01310475221.17 schema:affiliation https://www.grid.ac/institutes/grid.5100.4
    178 schema:familyName Halitzchi
    179 schema:givenName Ferdinand
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310475221.17
    181 rdf:type schema:Person
    182 sg:person.01337534000.32 schema:affiliation https://www.grid.ac/institutes/grid.5100.4
    183 schema:familyName Amuzescu
    184 schema:givenName Bogdan
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337534000.32
    186 rdf:type schema:Person
    187 sg:person.0740056540.19 schema:affiliation N93102be470e54a2e844a4110117f234d
    188 schema:familyName Constantinescu
    189 schema:givenName Andrei
    190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0740056540.19
    191 rdf:type schema:Person
    192 sg:pub.10.1007/s00404-014-3231-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016583613
    193 https://doi.org/10.1007/s00404-014-3231-7
    194 rdf:type schema:CreativeWork
    195 sg:pub.10.1007/s00441-014-1840-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1011389023
    196 https://doi.org/10.1007/s00441-014-1840-x
    197 rdf:type schema:CreativeWork
    198 sg:pub.10.1007/s10616-012-9441-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038923544
    199 https://doi.org/10.1007/s10616-012-9441-6
    200 rdf:type schema:CreativeWork
    201 sg:pub.10.1007/s12576-009-0050-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037892926
    202 https://doi.org/10.1007/s12576-009-0050-x
    203 rdf:type schema:CreativeWork
    204 sg:pub.10.1007/s12576-009-0080-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041084858
    205 https://doi.org/10.1007/s12576-009-0080-4
    206 rdf:type schema:CreativeWork
    207 sg:pub.10.1007/s12576-015-0421-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030208980
    208 https://doi.org/10.1007/s12576-015-0421-4
    209 rdf:type schema:CreativeWork
    210 sg:pub.10.1038/35000592 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036144012
    211 https://doi.org/10.1038/35000592
    212 rdf:type schema:CreativeWork
    213 sg:pub.10.1038/79449 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007162451
    214 https://doi.org/10.1038/79449
    215 rdf:type schema:CreativeWork
    216 sg:pub.10.1038/cddis.2013.93 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031106058
    217 https://doi.org/10.1038/cddis.2013.93
    218 rdf:type schema:CreativeWork
    219 sg:pub.10.1038/cgt.2012.30 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009806288
    220 https://doi.org/10.1038/cgt.2012.30
    221 rdf:type schema:CreativeWork
    222 sg:pub.10.1038/nbt1274 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028121369
    223 https://doi.org/10.1038/nbt1274
    224 rdf:type schema:CreativeWork
    225 sg:pub.10.1186/1471-2202-5-33 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053265151
    226 https://doi.org/10.1186/1471-2202-5-33
    227 rdf:type schema:CreativeWork
    228 sg:pub.10.1186/scrt444 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021531310
    229 https://doi.org/10.1186/scrt444
    230 rdf:type schema:CreativeWork
    231 sg:pub.10.2478/s11658-010-0017-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012021568
    232 https://doi.org/10.2478/s11658-010-0017-1
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1002/cyto.a.22402 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045598329
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1016/j.jacc.2007.07.041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048138798
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1016/j.stem.2008.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024126337
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1016/j.tibtech.2015.04.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028729000
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1080/14653240902974024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022131095
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1093/humrep/deh279 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042246019
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1095/biolreprod.105.046029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060111035
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1111/j.1582-4934.2004.tb00320.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1012020922
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1113/jphysiol.1980.sp013113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011811711
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1126/science.aaa5619 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042259520
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1152/ajpcell.00251.2009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011665653
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1152/ajpcell.00326.2011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012294622
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1200/jco.2000.18.2.307 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074577133
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1371/journal.pone.0053372 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006239776
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1371/journal.pone.0073816 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038913386
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1387/ijdb.092935md schema:sameAs https://app.dimensions.ai/details/publication/pub.1067191121
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1634/stemcells.2004-0149 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023081875
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1634/stemcells.2004-0213 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007233184
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.2174/138920111794295710 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069177780
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.3892/mmr.2011.499 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071520138
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.4061/2010/369578 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051071261
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.4161/org.22426 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072306763
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.5966/sctm.2013-0081 schema:sameAs https://app.dimensions.ai/details/publication/pub.1073577423
    279 rdf:type schema:CreativeWork
    280 https://www.grid.ac/institutes/grid.5100.4 schema:alternateName University of Bucharest
    281 schema:name Department Biophysics and Physiology, Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, 050095, Bucharest, Romania
    282 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...