Unyvero ITI® system for the clinical resolution of discrepancies in periprosthetic joint infection diagnosis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-10-08

AUTHORS

Silvia Zannoli, Andrea Sambri, Manuela Morotti, Martina Tassinari, Arianna Torri, Giuseppe Bianchi, Massimiliano De Paolis, Davide Maria Donati, Vittorio Sambri

ABSTRACT

ObjectivesThe Unyvero molecular assay was tested for the clinical resolution of discordant results, evaluating its role in prosthetic joint infection diagnosis.MethodsMultiplex PCR was performed on 45 samples from prosthesis treatment (either sonication or dithiothreitol). Analytical performance was compared to that of biofilm culture using Musculoskeletal Infection Society criteria as gold standard.ResultsUnyvero and biofilm culture showed similar agreement rates compared to the gold standard (34/43 and 32/43, respectively). Both methods showed six additional identifications compatible with true infection; five positive results from biofilm culture were deemed contaminations.ConclusionsThe Unyvero system showed good performances and a significantly shorter turnaround time compared to cultural methods, presenting an added value to PJI diagnosis even when performed following a composite approach. More... »

PAGES

39-42

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12306-019-00626-x

DOI

http://dx.doi.org/10.1007/s12306-019-00626-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1121577894

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/31595427


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