In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’ View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-07-10

AUTHORS

Babak ValizadehKaji, Ahmad Ershadi, Masoud Tohidfar

ABSTRACT

An efficient in vitro propagation is described for Punica granatum L. using shoot tip and nodal explants. The influence of two basal medium, WPM and MS, and different plant growth regulators was investigated on micropropagation of the Iranian pomegranate cultivars, ‘Malas Saveh’ and ‘Yousef Khani’. For proliferation stage, media supplemented with different concentrations (2.3, 4.7, 9.2 and 18.4 μM) of kinetin along with 0.54 μM NAA was used. WPM proved to be more efficient medium compared to MS. The best concentrations of kinetin were 4.7 μM for ‘Malas Saveh’ and 9.2 μM for ‘Yousef Khani’, resulting in the highest number of shoots per explants, shoot length and leaf number. For both cultivars, half-strength WPM medium supplemented with 5.4 μM NAA was most effective for rooting of shoots. Rooted plantlets were successfully acclimatized and transferred into soil. The micropropagated plants were morphologically uniform and exhibited similar growth characteristics and vegetative morphology to the mother plants. More... »

PAGES

597-603

References to SciGraph publications

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  • 2007-04-24. Agrobacterium-mediated transformation of the dwarf pomegranate (Punica granatum L. var. nana) in PLANT CELL REPORTS
  • 2008-11-26. In vitro plant regeneration from seedling explants of Stereospermumpersonatum D.C.: a medicinal tree in TREES
  • 2012-10-24. Biotechnological advances in pomegranate (Punica granatum L.) in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2009-11-25. Comparison of in vitro regeneration pathways in Punica granatum L. in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s12298-013-0193-3

    DOI

    http://dx.doi.org/10.1007/s12298-013-0193-3

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1013102818

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24431529


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