Isolation of Novel Exo-type β-Agarase from Gilvimarinus chinensis and High-level Secretory Production in Corynebacterium glutamicum View Full Text


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Article Info

DATE

2019-02

AUTHORS

Yong Jun Jeong, Jae Woong Choi, Min Soo Cho, Ki Jun Jeong

ABSTRACT

Agar, a major cell wall component in marine red macroalgae, has recently gained high interest as a potential renewable biomass. By the activity of β-agarase which cleaves the β-1,4-glycosidic bond, agarose can be hydrolyzed into neoagarobioses which have great potential in the cosmetics, food, and medical industries. Here, based on sequence homology analysis, we isolated a novel exotype β-agarase (EXB3) from Gilvimarinus chinensis which have putative glycoside hydrolase (GH) 50 domain. The optimum pH and temperature for the activity of EXB3 were pH 7.0 and 30°C, respectively. The Km and Vmax for agarose were 26 mg/mL and 126.8 U/mg, respectively, and the Kcat/Km value was 3.7 × 105 s-1M-1. Under the optimal condition (30°C and pH 7), it was clearly confirmed that neoagarobiose (NA2) was produced as a major product directly from agarose. For the large-scale production of EXB3, we also developed a secretory production platform in Corynebacterium glutamicum. During the fed-batch cultivation in 2 L-scale bioreactor, EXB3 was successfully produced in the culture medium as high as 458.3 mg/L, and EXB3 was purified from the culture supernatant with high purity and recovery yield (24%). More... »

PAGES

250-257

References to SciGraph publications

  • 2017-12. Neoagaro-oligosaccharide monomers inhibit inflammation in LPS-stimulated macrophages through suppression of MAPK and NF-κB pathways in SCIENTIFIC REPORTS
  • 2017. Predicting Secretory Proteins with SignalP in PROTEIN FUNCTION PREDICTION
  • 2014-01. High-level secretory production of recombinant single-chain variable fragment (scFv) in Corynebacterium glutamicum in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2008-02. Purification and characterization of a novel β-agarase, AgaA34, from Agarivorans albus YKW-34 in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2010-03. Overexpression and molecular characterization of Aga50D from Saccharophagus degradans 2-40: an exo-type β-agarase producing neoagarobiose in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2018-02. A Novel 3,6-anhydro-L-galactose Dehydrogenase Produced by a Newly Isolated Raoultella ornithinolytica B6-JMP12 in BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
  • 2018-05. Metabolic engineering of Corynebacterium glutamicum for fermentative production of chemicals in biorefinery in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2018-09. Cloning, Expression, and Characterization of a Thermotolerant β-agarase from Simiduia sp. SH-4 in BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
  • 2011-11. Overexpression and biochemical characterization of DagA from Streptomyces coelicolor A3(2): an endo-type β-agarase producing neoagarotetraose and neoagarohexaose in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2017-08. In vitro and in vivo investigation for biological activities of neoagarooligosaccharides prepared by hydrolyzing agar with β-agarase in BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
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    http://scigraph.springernature.com/pub.10.1007/s12257-018-0362-x

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    http://dx.doi.org/10.1007/s12257-018-0362-x

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