Cloning, Expression, and Characterization of a Thermotolerant β-agarase from Simiduia sp. SH-4 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-09

AUTHORS

Jae-Deog Kim, Dong-Geun Lee, Sang-Hyeon Lee

ABSTRACT

The gene coding for a thermotolerant β-agarase from an isolated Simiduia sp. SH-4 was cloned, recombinantly expressed, and characterized after purification. This gene was sequenced after cassette mediated polymerase chain reaction and composed of an open reading frame of 1,809 base pairs, encoding a protein of 66.2 kilodaltons comprising of 602 amino acid residues. The amino acids sequence showed 74% homology with β-agarase of Simiduia agarivorans. A new β-agarase gene corresponding to mature protein of 577 amino acids was recombinantly expressed and purified by chitin bead column to homogeneity. The maximal specific activity was 505.07 U/mg at 50oC in Tris/HCl (pH 6.0) buffer. Recombinant β-agarase hydrolyzed agar into neoagarotetraose (57%) and neoagarohexaose (43%). It generated products from melted and non-melted powder agar and agarose at 30-50oC, meaning cheap agar materials could be used with energy- and costsavings. Thus, recombinant β-agarase could be used for industrial production of neoagarotetraose and neoagarohexaose. More... »

PAGES

525-531

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12257-018-0072-4

DOI

http://dx.doi.org/10.1007/s12257-018-0072-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1109806979


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