Bioconversion of acrylonitrile to acrylamide using polyacrylamide entrapped cells of Rhodococcus rhodochrous PA-34 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-09

AUTHORS

J. Raj, S. Prasad, N. N. Sharma, T. C. Bhalla

ABSTRACT

The nitrile hydratase (NHase) of Rhodococcus rhodochrous PA-34 catalyzed the conversion of acrylonitrile to acrylamide. The resting cells (having NHase activity) (8 %; 1 mL corresponds to 22 mg dry cell mass, DCM) were immobilized in polyacrylamide gel containing 12.5 % acrylamide, 0.6 % bisacrylamide, 0.2 % diammonium persulfate and 0.4 % TEMED. The polyacrylamide entrapped cells (1.12 mg DCM/mL) completely converted acrylonitrile in 3 h at 10 °C, using 0.1 mol/L potassium phosphate buffer. In a partitioned fed batch reactor, 432 g/L acrylamide was accumulated after 1 d. The polyacrylamide discs were recycled up to 3×; 405, 210 and 170 g/L acrylamide was produced in 1st, 2nd and 3rd recycling reactions. In four cycles, a total of 1217 g acrylamide was produced by recycling the same mass of entrapped cells. More... »

PAGES

442-446

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12223-010-0074-x

DOI

http://dx.doi.org/10.1007/s12223-010-0074-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050549112

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20941578


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