Presynaptic Release of Glutamate by Heteroexchange Under Altered Gravity Conditions View Full Text


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Article Info

DATE

2008-07-15

AUTHORS

Tatiana Borisova, Natalia Krisanova

ABSTRACT

The study focused on the release of glutamate by reversal of Na + -dependent glutamate transporters that was investigated in cortical synaptosomes isolated from Wistar rats subjected to centrifuge-induced hypergravity of 10×g for 1 h. Transportable competitive inhibitor of glutamate transporters—DL-threo-beta-hydroxyaspartate (DL-THA) induced the release of L-[14C]glutamate via heteroexchange that consisted of 22.5 ± 1.7% of total label in normal gravity and 23.7 ± 1.7% in altered gravity conditions. Inhibition of the ability of synaptic vesicles to accumulate the neuromediator by preliminary treatment of synaptosomes with the protonophore (FCCP) augmented the release of cytosolic L-[14C]glutamate evoked by DL-THA from 44.0 ± 2.0% to 52.0 ± 2.3% of total label for G-loaded animals as compared to controls. Thus, combined application of DL-THA and FCCP revealed the increase in transporter-mediated release of glutamate that might be evidence for hypoxic injury of neurons. More... »

PAGES

197

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12217-008-9049-9

DOI

http://dx.doi.org/10.1007/s12217-008-9049-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042097159


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