A modified thrombin generation test for investigating very low levels of factor VIII activity in hemophilia A View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-11-25

AUTHORS

Tomoko Matsumoto, Keiji Nogami, Kenichi Ogiwara, Midori Shima

ABSTRACT

Discrepancies between low levels of FVIII:C and clinical symptoms in severe hemophilia A are well-known. We have recently demonstrated that levels of FVIII:C < 0.2 IU/dl were consistent with clinical phenotype by clot waveform analysis, suggesting that precise measurement of very low levels of FVIII:C was clinically important. Thrombin generation tests (TGTs) triggered by tissue factor (TF) have been recently utilized to monitor coagulation function in hemophilia A. We examined whether TGT was useful for evaluating hemophilia A patients with very low levels of FVIII:C. TGTs in 40 hemophilia A plasmas with FVIII:C < 0.2–17 IU/dl (measured by clot waveform analysis using MDA-II™) were performed using TF and/or ellagic acid (ELG). The lagtime in ELG-TGT at very low levels of FVIII:C was shortened dose-dependently, whilst this parameter in TF-TGT was not significantly affected. Other parameters (endogenous thrombin potential, peak thrombin, time to peak) correlated with FVIII:C levels to some extent in both assays (r = 0.4–0.7). Using a TF/ELG mixture in TGT, however, the correlation coefficients increased to ~0.85. TGT parameters correlated well with levels of FVIII:C > 0.2 IU/dl, although the lagtime was not especially informative. We conclude that modified TGT, using a TF/ELG mixture as the trigger, is useful for monitoring coagulation function at very low levels of FVIII:C in hemophilia A. More... »

PAGES

576-582

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12185-009-0450-y

DOI

http://dx.doi.org/10.1007/s12185-009-0450-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009175483

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19937483


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blood Coagulation Tests", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ellagic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Factor VIII", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hemophilia A", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thrombin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thromboplastin", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara634-8522, Nara, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410814.8", 
          "name": [
            "Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara634-8522, Nara, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matsumoto", 
        "givenName": "Tomoko", 
        "id": "sg:person.0742772162.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742772162.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara634-8522, Nara, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410814.8", 
          "name": [
            "Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara634-8522, Nara, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nogami", 
        "givenName": "Keiji", 
        "id": "sg:person.0603447532.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603447532.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara634-8522, Nara, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410814.8", 
          "name": [
            "Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara634-8522, Nara, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ogiwara", 
        "givenName": "Kenichi", 
        "id": "sg:person.01034124532.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034124532.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara634-8522, Nara, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410814.8", 
          "name": [
            "Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara634-8522, Nara, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shima", 
        "givenName": "Midori", 
        "id": "sg:person.0705772523.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0705772523.06"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2009-11-25", 
    "datePublishedReg": "2009-11-25", 
    "description": "Discrepancies between low levels of FVIII:C and clinical symptoms in severe hemophilia A are well-known. We have recently demonstrated that levels of FVIII:C < 0.2 IU/dl were consistent with clinical phenotype by clot waveform analysis, suggesting that precise measurement of very low levels of FVIII:C was clinically important. Thrombin generation tests (TGTs) triggered by tissue factor (TF) have been recently utilized to monitor coagulation function in hemophilia A. We examined whether TGT was useful for evaluating hemophilia A patients with very low levels of FVIII:C. TGTs in 40 hemophilia A plasmas with FVIII:C < 0.2\u201317 IU/dl (measured by clot waveform analysis using MDA-II\u2122) were performed using TF and/or ellagic acid (ELG). The lagtime in ELG-TGT at very low levels of FVIII:C was shortened dose-dependently, whilst this parameter in TF-TGT was not significantly affected. Other parameters (endogenous thrombin potential, peak thrombin, time to peak) correlated with FVIII:C levels to some extent in both assays (r = 0.4\u20130.7). Using a TF/ELG mixture in TGT, however, the correlation coefficients increased to ~0.85. TGT parameters correlated well with levels of FVIII:C > 0.2 IU/dl, although the lagtime was not especially informative. We conclude that modified TGT, using a TF/ELG mixture as the trigger, is useful for monitoring coagulation function at very low levels of FVIII:C in hemophilia A.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s12185-009-0450-y", 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6017438", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1076985", 
        "issn": [
          "0925-5710", 
          "1865-3774"
        ], 
        "name": "International Journal of Hematology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "90"
      }
    ], 
    "keywords": [
      "thrombin generation test", 
      "IU/dl", 
      "levels of FVIII", 
      "tissue factor", 
      "coagulation function", 
      "hemophilia A", 
      "severe haemophilia A", 
      "clot waveform analysis", 
      "factor VIII activity", 
      "ellagic acid", 
      "low levels", 
      "TGT parameters", 
      "clinical symptoms", 
      "generation test", 
      "clinical phenotype", 
      "hemophilia A.", 
      "VIII activity", 
      "FVIII", 
      "C levels", 
      "dl", 
      "hemophilia", 
      "waveform analysis", 
      "lagtime", 
      "levels", 
      "patients", 
      "symptoms", 
      "dose", 
      "correlation coefficient", 
      "phenotype", 
      "test", 
      "assays", 
      "triggers", 
      "function", 
      "factors", 
      "plasma", 
      "activity", 
      "A.", 
      "acid", 
      "extent", 
      "discrepancy", 
      "analysis", 
      "parameters", 
      "measurements", 
      "precise measurement", 
      "mixture", 
      "coefficient"
    ], 
    "name": "A modified thrombin generation test for investigating very low levels of factor VIII activity in hemophilia A", 
    "pagination": "576-582", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1009175483"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s12185-009-0450-y"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19937483"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s12185-009-0450-y", 
      "https://app.dimensions.ai/details/publication/pub.1009175483"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-08-04T16:57", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_479.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s12185-009-0450-y"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12185-009-0450-y'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12185-009-0450-y'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12185-009-0450-y'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12185-009-0450-y'


 

This table displays all metadata directly associated to this object as RDF triples.

158 TRIPLES      20 PREDICATES      78 URIs      70 LITERALS      14 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s12185-009-0450-y schema:about N0325eda2d8ad40d4959041d942e3e46b
2 N371c72e35c5743f8bea2dec7f51bf032
3 N58703c255f4e429e8f3923bb718df569
4 N5d3d7fd07d1744ceb0cddca01f3f7f07
5 N7f0caf898b2d4641bdc289a66403d690
6 Nd7fbcb217e2c4cc6a31d8d1ad9c6f2cc
7 Nf2f02d05a65d472da52c830105a9c160
8 anzsrc-for:11
9 anzsrc-for:1103
10 schema:author N9a58f523da60463da71388ae6041d5f4
11 schema:datePublished 2009-11-25
12 schema:datePublishedReg 2009-11-25
13 schema:description Discrepancies between low levels of FVIII:C and clinical symptoms in severe hemophilia A are well-known. We have recently demonstrated that levels of FVIII:C < 0.2 IU/dl were consistent with clinical phenotype by clot waveform analysis, suggesting that precise measurement of very low levels of FVIII:C was clinically important. Thrombin generation tests (TGTs) triggered by tissue factor (TF) have been recently utilized to monitor coagulation function in hemophilia A. We examined whether TGT was useful for evaluating hemophilia A patients with very low levels of FVIII:C. TGTs in 40 hemophilia A plasmas with FVIII:C < 0.2–17 IU/dl (measured by clot waveform analysis using MDA-II™) were performed using TF and/or ellagic acid (ELG). The lagtime in ELG-TGT at very low levels of FVIII:C was shortened dose-dependently, whilst this parameter in TF-TGT was not significantly affected. Other parameters (endogenous thrombin potential, peak thrombin, time to peak) correlated with FVIII:C levels to some extent in both assays (r = 0.4–0.7). Using a TF/ELG mixture in TGT, however, the correlation coefficients increased to ~0.85. TGT parameters correlated well with levels of FVIII:C > 0.2 IU/dl, although the lagtime was not especially informative. We conclude that modified TGT, using a TF/ELG mixture as the trigger, is useful for monitoring coagulation function at very low levels of FVIII:C in hemophilia A.
14 schema:genre article
15 schema:isAccessibleForFree false
16 schema:isPartOf N6541c9e067c04ac8ac98c3bd392f10c8
17 Na95fbe36c1ef4890a5fdfe093ea88d9a
18 sg:journal.1076985
19 schema:keywords A.
20 C levels
21 FVIII
22 IU/dl
23 TGT parameters
24 VIII activity
25 acid
26 activity
27 analysis
28 assays
29 clinical phenotype
30 clinical symptoms
31 clot waveform analysis
32 coagulation function
33 coefficient
34 correlation coefficient
35 discrepancy
36 dl
37 dose
38 ellagic acid
39 extent
40 factor VIII activity
41 factors
42 function
43 generation test
44 hemophilia
45 hemophilia A
46 hemophilia A.
47 lagtime
48 levels
49 levels of FVIII
50 low levels
51 measurements
52 mixture
53 parameters
54 patients
55 phenotype
56 plasma
57 precise measurement
58 severe haemophilia A
59 symptoms
60 test
61 thrombin generation test
62 tissue factor
63 triggers
64 waveform analysis
65 schema:name A modified thrombin generation test for investigating very low levels of factor VIII activity in hemophilia A
66 schema:pagination 576-582
67 schema:productId N12c1a06e1016436a8faf8e20ec8baa2b
68 N399c87ebb45c42f6b77ddebec100e0a1
69 Nac7804308f004c09ba107b25665b5d03
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009175483
71 https://doi.org/10.1007/s12185-009-0450-y
72 schema:sdDatePublished 2022-08-04T16:57
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher N1278b8378cab4004a25f743c0f4d169b
75 schema:url https://doi.org/10.1007/s12185-009-0450-y
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N0325eda2d8ad40d4959041d942e3e46b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Hemophilia A
81 rdf:type schema:DefinedTerm
82 N0c9ed56597184308a7a40ee49486c3a5 rdf:first sg:person.01034124532.95
83 rdf:rest Nacfe01ff71a54df59301d537c88ba44f
84 N10e3c56172324a5dac60b43a8d1a12cf rdf:first sg:person.0603447532.27
85 rdf:rest N0c9ed56597184308a7a40ee49486c3a5
86 N1278b8378cab4004a25f743c0f4d169b schema:name Springer Nature - SN SciGraph project
87 rdf:type schema:Organization
88 N12c1a06e1016436a8faf8e20ec8baa2b schema:name pubmed_id
89 schema:value 19937483
90 rdf:type schema:PropertyValue
91 N371c72e35c5743f8bea2dec7f51bf032 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Thromboplastin
93 rdf:type schema:DefinedTerm
94 N399c87ebb45c42f6b77ddebec100e0a1 schema:name dimensions_id
95 schema:value pub.1009175483
96 rdf:type schema:PropertyValue
97 N58703c255f4e429e8f3923bb718df569 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Ellagic Acid
99 rdf:type schema:DefinedTerm
100 N5d3d7fd07d1744ceb0cddca01f3f7f07 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Humans
102 rdf:type schema:DefinedTerm
103 N6541c9e067c04ac8ac98c3bd392f10c8 schema:issueNumber 5
104 rdf:type schema:PublicationIssue
105 N7f0caf898b2d4641bdc289a66403d690 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Thrombin
107 rdf:type schema:DefinedTerm
108 N9a58f523da60463da71388ae6041d5f4 rdf:first sg:person.0742772162.09
109 rdf:rest N10e3c56172324a5dac60b43a8d1a12cf
110 Na95fbe36c1ef4890a5fdfe093ea88d9a schema:volumeNumber 90
111 rdf:type schema:PublicationVolume
112 Nac7804308f004c09ba107b25665b5d03 schema:name doi
113 schema:value 10.1007/s12185-009-0450-y
114 rdf:type schema:PropertyValue
115 Nacfe01ff71a54df59301d537c88ba44f rdf:first sg:person.0705772523.06
116 rdf:rest rdf:nil
117 Nd7fbcb217e2c4cc6a31d8d1ad9c6f2cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Blood Coagulation Tests
119 rdf:type schema:DefinedTerm
120 Nf2f02d05a65d472da52c830105a9c160 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Factor VIII
122 rdf:type schema:DefinedTerm
123 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
124 schema:name Medical and Health Sciences
125 rdf:type schema:DefinedTerm
126 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
127 schema:name Clinical Sciences
128 rdf:type schema:DefinedTerm
129 sg:grant.6017438 http://pending.schema.org/fundedItem sg:pub.10.1007/s12185-009-0450-y
130 rdf:type schema:MonetaryGrant
131 sg:journal.1076985 schema:issn 0925-5710
132 1865-3774
133 schema:name International Journal of Hematology
134 schema:publisher Springer Nature
135 rdf:type schema:Periodical
136 sg:person.01034124532.95 schema:affiliation grid-institutes:grid.410814.8
137 schema:familyName Ogiwara
138 schema:givenName Kenichi
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034124532.95
140 rdf:type schema:Person
141 sg:person.0603447532.27 schema:affiliation grid-institutes:grid.410814.8
142 schema:familyName Nogami
143 schema:givenName Keiji
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603447532.27
145 rdf:type schema:Person
146 sg:person.0705772523.06 schema:affiliation grid-institutes:grid.410814.8
147 schema:familyName Shima
148 schema:givenName Midori
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0705772523.06
150 rdf:type schema:Person
151 sg:person.0742772162.09 schema:affiliation grid-institutes:grid.410814.8
152 schema:familyName Matsumoto
153 schema:givenName Tomoko
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742772162.09
155 rdf:type schema:Person
156 grid-institutes:grid.410814.8 schema:alternateName Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara634-8522, Nara, Japan
157 schema:name Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara634-8522, Nara, Japan
158 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...