Solid-state NMR sequential assignments of the amyloid core of full-length Sup35p View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-10

AUTHORS

Anne K. Schütz, Birgit Habenstein, Nina Luckgei, Luc Bousset, Yannick Sourigues, Anders B. Nielsen, Ronald Melki, Anja Böckmann, Beat H. Meier

ABSTRACT

Sup35p is a yeast prion and is responsible for the [PSI(+)] trait in Saccharomyces cerevisiae. With 685 amino acids, full-length soluble and fibrillar Sup35p are challenging targets for structural biology as they cannot be investigated by X-ray crystallography or NMR in solution. We present solid-state NMR studies of fibrils formed by the full-length Sup35 protein. We detect an ordered and rigid core of the protein that gives rise to narrow and strong peaks, while large parts of the protein show either static disorder or dynamics on time scales which interfere with dipolar polarization transfer or shorten the coherence lifetime. Thus, only a small subset of resonances is observed in 3D spectra. Here we describe in detail the sequential assignments of the 22 residues for which resonances are observed in 3D spectra: their chemical shifts mostly corresponding to β-sheet secondary structure. We suspect that these residues form the amyloid core of the fibril. More... »

PAGES

349-356

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12104-013-9515-1

DOI

http://dx.doi.org/10.1007/s12104-013-9515-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027673625

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23943018


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