Ontology type: schema:ScholarlyArticle Open Access: True
2021-06-02
AUTHORSSuguru Nakashiki, Satoshi Miuma, Hiroyuki Mishima, Hiroshi Masumoto, Masaaki Hidaka, Akihiko Soyama, Yasuko Kanda, Masanori Fukushima, Masafumi Haraguchi, Ryu Sasaki, Hisamitsu Miyaaki, Tatsuki Ichikawa, Mitsuhisa Takatsuki, Susumu Eguchi, Koh-ichiro Yoshiura, Kazuhiko Nakao
ABSTRACTBackgroundExtracellular vesicles (EVs) have recently attracted attention as novel diagnostic biomarkers and therapeutic tools. Several reports have correlated blood EVs with liver diseases. However, blood EVs do not reflect the liver state as it contains other systemically circulating EVs. Therefore, we focused on bile EVs, which are secreted directly from the liver, for the identification of potential biomarkers of liver failure.MethodsBile samples were collected from liver transplant recipients (n = 21) diagnosed with end-stage liver disease (ESLD) and donors (normal liver, NL; n = 18) during transplantation. Bile EVs were extracted using ultracentrifugation.ResultsNanoparticle tracking analysis showed that bile EV concentration was significantly higher in recipients than in donors. Among recipients, bile EV concentration was remarkably higher in those with hepatocellular carcinoma. Next-generation sequencing revealed 461 and 465 types of microRNAs (miRNAs) in donor and recipient bile EVs, respectively, with no significant difference in diversity between the groups. Among 43 high-expression miRNAs, the expression of 86.0% of the miRNAs was higher in the bile EVs of recipients than in those of donors. Quantitative PCR validation showed that the levels of miR-17, miR-92a, miR-25, miR-423, and miR-451a significantly increased in bile EVs of recipients. Levels of miR-17 were remarkably higher in recipients with alcoholic ESLD.ConclusionsSecretion of EVs into the bile and their miRNA content increase in the ESLD state. Additionally, miRNA levels in bile EVs are not correlated with those in serum EVs. Bile EVs could be promising novel biomarkers for liver diseases. More... »
PAGES821-830
http://scigraph.springernature.com/pub.10.1007/s12072-021-10196-5
DOIhttp://dx.doi.org/10.1007/s12072-021-10196-5
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1138525347
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/34076850
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Clinical Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Bile",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Biomarkers",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "End Stage Liver Disease",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Extracellular Vesicles",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "MicroRNAs",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Nakashiki",
"givenName": "Suguru",
"id": "sg:person.013302353154.28",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013302353154.28"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Miuma",
"givenName": "Satoshi",
"id": "sg:person.01223547024.84",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01223547024.84"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Human Genetics, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Human Genetics, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Mishima",
"givenName": "Hiroyuki",
"id": "sg:person.0643355356.48",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643355356.48"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Biochemical Research Support Center, Nagasaki University School of Medicine, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Biochemical Research Support Center, Nagasaki University School of Medicine, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Masumoto",
"givenName": "Hiroshi",
"id": "sg:person.01073202740.31",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073202740.31"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8523, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8523, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Hidaka",
"givenName": "Masaaki",
"id": "sg:person.0615445574.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615445574.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8523, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8523, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Soyama",
"givenName": "Akihiko",
"id": "sg:person.0754357512.20",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754357512.20"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Kanda",
"givenName": "Yasuko",
"id": "sg:person.016640133647.51",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016640133647.51"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Fukushima",
"givenName": "Masanori",
"id": "sg:person.01343164751.24",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343164751.24"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Haraguchi",
"givenName": "Masafumi",
"id": "sg:person.0676623047.88",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0676623047.88"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Sasaki",
"givenName": "Ryu",
"id": "sg:person.0745303701.69",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0745303701.69"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Miyaaki",
"givenName": "Hisamitsu",
"id": "sg:person.0674221772.77",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674221772.77"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gastroenterology Shinti, Nagasaki Harbor Medical Center City Hospital, 6-39, 850-8555, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Department of Gastroenterology Shinti, Nagasaki Harbor Medical Center City Hospital, 6-39, 850-8555, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Ichikawa",
"givenName": "Tatsuki",
"id": "sg:person.01170332131.55",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170332131.55"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Digestive and General Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, 903-0215, Nishihara, Okinawa, Japan",
"id": "http://www.grid.ac/institutes/grid.267625.2",
"name": [
"Department of Digestive and General Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, 903-0215, Nishihara, Okinawa, Japan"
],
"type": "Organization"
},
"familyName": "Takatsuki",
"givenName": "Mitsuhisa",
"id": "sg:person.01300545517.89",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300545517.89"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8523, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8523, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Eguchi",
"givenName": "Susumu",
"id": "sg:person.01224623274.12",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224623274.12"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Human Genetics, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Human Genetics, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Yoshiura",
"givenName": "Koh-ichiro",
"id": "sg:person.01304011603.19",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304011603.19"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan",
"id": "http://www.grid.ac/institutes/grid.174567.6",
"name": [
"Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan"
],
"type": "Organization"
},
"familyName": "Nakao",
"givenName": "Kazuhiko",
"id": "sg:person.07616434412.60",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07616434412.60"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s00441-012-1428-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010042378",
"https://doi.org/10.1007/s00441-012-1428-2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s12072-019-09993-w",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1122003008",
"https://doi.org/10.1007/s12072-019-09993-w"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s12916-014-0145-y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009310059",
"https://doi.org/10.1186/s12916-014-0145-y"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s12072-012-9341-z",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016636482",
"https://doi.org/10.1007/s12072-012-9341-z"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s12072-018-9897-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1107497260",
"https://doi.org/10.1007/s12072-018-9897-3"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s12967-015-0623-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044034054",
"https://doi.org/10.1186/s12967-015-0623-9"
],
"type": "CreativeWork"
}
],
"datePublished": "2021-06-02",
"datePublishedReg": "2021-06-02",
"description": "BackgroundExtracellular vesicles (EVs) have recently attracted attention as novel diagnostic biomarkers and therapeutic tools. Several reports have correlated blood EVs with liver diseases. However, blood EVs do not reflect the liver state as it contains other systemically circulating EVs. Therefore, we focused on bile EVs, which are secreted directly from the liver, for the identification of potential biomarkers of liver failure.MethodsBile samples were collected from liver transplant recipients (n\u2009=\u200921) diagnosed with end-stage liver disease (ESLD) and donors (normal liver, NL; n\u2009=\u200918) during transplantation. Bile EVs were extracted using ultracentrifugation.ResultsNanoparticle tracking analysis showed that bile EV concentration was significantly higher in recipients than in donors. Among recipients, bile EV concentration was remarkably higher in those with hepatocellular carcinoma. Next-generation sequencing revealed 461 and 465 types of microRNAs (miRNAs) in donor and recipient bile EVs, respectively, with no significant difference in diversity between the groups. Among 43 high-expression miRNAs, the expression of 86.0% of the miRNAs was higher in the bile EVs of recipients than in those of donors. Quantitative PCR validation showed that the levels of miR-17, miR-92a, miR-25, miR-423, and miR-451a significantly increased in bile EVs of recipients. Levels of miR-17 were remarkably higher in recipients with alcoholic ESLD.ConclusionsSecretion of EVs into the bile and their miRNA content increase in the ESLD state. Additionally, miRNA levels in bile EVs are not correlated with those in serum EVs. Bile EVs could be promising novel biomarkers for liver diseases.",
"genre": "article",
"id": "sg:pub.10.1007/s12072-021-10196-5",
"inLanguage": "en",
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1038556",
"issn": [
"1936-0533",
"1936-0541"
],
"name": "Hepatology International",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "3",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "15"
}
],
"keywords": [
"end-stage liver disease",
"liver disease",
"alcoholic end-stage liver disease",
"end-stage liver disease patients",
"liver transplant recipients",
"liver disease patients",
"EV concentration",
"novel diagnostic biomarkers",
"miR-17",
"MethodsBile samples",
"transplant recipients",
"liver failure",
"disease patients",
"types of microRNAs",
"hepatocellular carcinoma",
"novel biomarkers",
"serum EVs",
"high-expression miRNAs",
"diagnostic biomarkers",
"quantitative PCR validation",
"potential biomarkers",
"miR-25",
"miR-451a",
"therapeutic tool",
"miR-92a",
"miR-423",
"liver state",
"recipients",
"disease",
"next-generation sequencing",
"biomarkers",
"significant differences",
"extracellular vesicles",
"miRNA levels",
"PCR validation",
"donors",
"transplantation",
"patients",
"carcinoma",
"bile",
"levels",
"liver",
"miRNAs",
"tracking analysis",
"report",
"vesicles",
"microRNAs",
"failure",
"group",
"expression",
"concentration",
"differences",
"sequencing",
"increase",
"ultracentrifugation",
"identification",
"samples",
"types",
"analysis",
"validation",
"tool",
"state",
"attention",
"content",
"diversity",
"content increases",
"eV"
],
"name": "Bile extracellular vesicles from end-stage liver disease patients show altered microRNA content",
"pagination": "821-830",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1138525347"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s12072-021-10196-5"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"34076850"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s12072-021-10196-5",
"https://app.dimensions.ai/details/publication/pub.1138525347"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:38",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_895.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s12072-021-10196-5"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12072-021-10196-5'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12072-021-10196-5'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12072-021-10196-5'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12072-021-10196-5'
This table displays all metadata directly associated to this object as RDF triples.
294 TRIPLES
22 PREDICATES
105 URIs
91 LITERALS
13 BLANK NODES