BAC-end Sequence Analysis and a Draft Physical Map of the Common Bean (Phaseolus vulgaris L.) Genome View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-03

AUTHORS

Jessica A. Schlueter, Jose Luis Goicoechea, Kristi Collura, Navdeep Gill, Jer-Young Lin, Yeisoo Yu, Dave Kudrna, Andrea Zuccolo, C. Eduardo Vallejos, Monica Muñoz-Torres, Matthew W. Blair, Joe Tohme, Jeff Tomkins, Phillip McClean, Rod A. Wing, Scott A. Jackson

ABSTRACT

Common bean (Phaseolus vulgaris L.) is a legume that is an important source of dietary protein in developing countries throughout the world. Utilizing the G19833 BAC library for P. vulgaris from Clemson University, 89,017 BAC-end sequences were generated giving 62,588,675 base pairs of genomic sequence covering approximately 9.54% of the genome. Analysis of these sequences in combination with 1,404 shotgun sequences from the cultivar Bat7 revealed that approximately 49.2% of the genome contains repetitive sequence and 29.3% is genic. Compared to other legume BAC-end sequencing projects, it appears that P. vulgaris has higher predicted levels of repetitive sequence, but this may be due to a more intense identification strategy combining both similarity-based matches as well as de novo identification of repeats. In addition, fingerprints for 41,717 BACs were obtained and assembled into a draft physical map consisting of 1,183 clone contigs and 6,385 singletons with ~9x coverage of the genome. More... »

PAGES

40-48

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12042-007-9003-9

DOI

http://dx.doi.org/10.1007/s12042-007-9003-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034386325


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