AFLP studies on downy-mildew-resistant and downy-mildew-susceptible genotypes of opium poppy View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-02-22

AUTHORS

Mukesh K. Dubey, Ajit K. Shasany, Omp. Dhawan, Ashutosh K. Shukla, Suman P. S. Khanuja

ABSTRACT

Downy mildew (DM) caused by Peronospora arborescens, is a serious disease in opium poppy (Papaver somniferum), which has a world-wide spread. The establishment of DM-resistant cultivars appears to be a sustainable way to control the disease. In this paper, we present the results of a study aimed at the identification of amplified fragment length polymorphism (AFLP) markers for DM-resistance in opium poppy. Three opium poppy genotypes (inbred over about 10 years): Pps-1 (DM-resistant), Jawahar-16 (DM-susceptible) and H-9 (DM-susceptible) were crossed in a diallel manner and the F1 progeny along with the parents were subjected to AFLP analysis of chloroplast (cp) and nuclear DNA with seven and nine EcoRI / MseI primer combinations, respectively. cpDNA AFLP analysis identified 24 Pps-1 (DM-resistant)-specific unique fragments that were found to be maternally inherited in both the crosses, Pps-1 × Jawahar-16 and Pps-1 × H-9. In the case of nuclear DNA AFLP analysis, it was found that 17 fragments inherited from Pps-1 were common to the reciprocal crosses of both (i) Pps-1 and Jawahar-16 as well as (ii) Pps-1 and H-9. This is the first molecular investigation on the identification of polymorphism between DM-resistant and DM-susceptible opium poppy genotypes and development of DM-resistant opium poppy genotype-specific AFLP markers. These AFLP markers could be used in future genetic studies for analysis of linkage to the downy mildew resistance trait. More... »

PAGES

9-19

References to SciGraph publications

  • 1999-03. ISPMB Sixth Congress First Announcement in PLANT MOLECULAR BIOLOGY REPORTER
  • 2002-10-22. Identification of DNA markers of tobacco linked to bacterial wilt resistance in THEORETICAL AND APPLIED GENETICS
  • 2008-09-26. Downy mildew resistance in opium poppy: resistance sources, inheritance pattern, genetic variability and strategies for crop improvement in EUPHYTICA
  • 1992. Chloroplast DNA and the Study of Plant Phylogeny: Present Status and Future Prospects in MOLECULAR SYSTEMATICS OF PLANTS
  • 1999-03. Rapid Isolation of DNA from Dry and Fresh Samples of Plants Producing Large Amounts of Secondary Metabolites and Essential Oils in PLANT MOLECULAR BIOLOGY REPORTER
  • 2002-06-26. Cloning of AFLP markers linked to resistance to Peronosclerospora sorghi in maize in MOLECULAR GENETICS AND GENOMICS
  • 2004-11-13. Identification of molecular markers linked to the mildew resistance gene Pl-d in apple in THEORETICAL AND APPLIED GENETICS
  • 2004-08-21. Tagging and mapping of a rice gall midge resistance gene, Gm8, and development of SCARs for use in marker-aided selection and gene pyramiding in THEORETICAL AND APPLIED GENETICS
  • 1996-06. Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA mapping techniques in PLANT MOLECULAR BIOLOGY REPORTER
  • 1998. The Origin and Evolution of Plastids and Their Genomes in MOLECULAR SYSTEMATICS OF PLANTS II
  • 1999-05. A rapid method to score plastid haplotypes in red seaweeds and its use in determining parental inheritance of plastids in the red alga Bostrychia (Ceramiales) in HYDROBIOLOGIA
  • 1995-01. A high-resolution map of the vicinity of the R1 locus on chromosome V of potato based on RFLP and AFLP markers in MOLECULAR GENETICS AND GENOMICS
  • 1994-08. Chloroplast DNA as an evolutionary marker in thePhaseolus vulgaris complex in THEORETICAL AND APPLIED GENETICS
  • 1990-02. Paternal inheritance of plastids in the genus Daucus in MOLECULAR GENETICS AND GENOMICS
  • 1984-12. A rapid inexpensive method for the isolation of restrictable mitochondrial DNA from various plant sources in PLANT CELL REPORTS
  • 2000-01. Maternal inheritance of chloroplasts in the horsetail Equisetum variegatum (Schleich.) in CURRENT GENETICS
  • 2000-06. Association of AFLP markers with downy mildew resistance in autotetraploid alfalfa in MOLECULAR BREEDING
  • 1998-06. A Method for Isolation of Chloroplast DNA and Mitochondrial DNA from Sunflower in PLANT MOLECULAR BIOLOGY REPORTER
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s12041-010-0015-0

    DOI

    http://dx.doi.org/10.1007/s12041-010-0015-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1002889547

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20505242


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amplified Fragment Length Polymorphism Analysis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Nucleus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Crosses, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fatty Acids, Unsaturated", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Markers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genotype", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hybridization, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Immunity, Innate", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Inheritance Patterns", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Opium", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Papaver", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Peronospora", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plant Diseases", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Genetics and Plant Breeding Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Genetics and Plant Breeding Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dubey", 
            "givenName": "Mukesh K.", 
            "id": "sg:person.01346545715.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346545715.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shasany", 
            "givenName": "Ajit K.", 
            "id": "sg:person.01207061437.96", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207061437.96"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Genetics and Plant Breeding Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Genetics and Plant Breeding Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dhawan", 
            "givenName": "Omp.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shukla", 
            "givenName": "Ashutosh K.", 
            "id": "sg:person.0746716663.99", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746716663.99"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Khanuja", 
            "givenName": "Suman P. S.", 
            "id": "sg:person.010601233534.89", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010601233534.89"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/978-1-4615-5419-6_13", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033981105", 
              "https://doi.org/10.1007/978-1-4615-5419-6_13"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00269301", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005608283", 
              "https://doi.org/10.1007/bf00269301"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02684905", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028727120", 
              "https://doi.org/10.1007/bf02684905"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4615-3276-7_1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022795456", 
              "https://doi.org/10.1007/978-1-4615-3276-7_1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1007528101452", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020688090", 
              "https://doi.org/10.1023/a:1007528101452"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-004-1836-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035198430", 
              "https://doi.org/10.1007/s00122-004-1836-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00391760", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035153226", 
              "https://doi.org/10.1007/bf00391760"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1009672008702", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021074442", 
              "https://doi.org/10.1023/a:1009672008702"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1017213630972", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016322897", 
              "https://doi.org/10.1023/a:1017213630972"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-004-1774-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030858521", 
              "https://doi.org/10.1007/s00122-004-1774-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00438-002-0713-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001077092", 
              "https://doi.org/10.1007/s00438-002-0713-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1003706931897", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027333886", 
              "https://doi.org/10.1023/a:1003706931897"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1007487806583", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033208159", 
              "https://doi.org/10.1023/a:1007487806583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10681-008-9804-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012941598", 
              "https://doi.org/10.1007/s10681-008-9804-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01253966", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023839901", 
              "https://doi.org/10.1007/bf01253966"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002940050008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048144039", 
              "https://doi.org/10.1007/s002940050008"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-002-1096-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052498237", 
              "https://doi.org/10.1007/s00122-002-1096-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00290238", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001933298", 
              "https://doi.org/10.1007/bf00290238"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2010-02-22", 
        "datePublishedReg": "2010-02-22", 
        "description": "Downy mildew (DM) caused by Peronospora arborescens, is a serious disease in opium poppy (Papaver somniferum), which has a world-wide spread. The establishment of DM-resistant cultivars appears to be a sustainable way to control the disease. In this paper, we present the results of a study aimed at the identification of amplified fragment length polymorphism (AFLP) markers for DM-resistance in opium poppy. Three opium poppy genotypes (inbred over about 10 years): Pps-1 (DM-resistant), Jawahar-16 (DM-susceptible) and H-9 (DM-susceptible) were crossed in a diallel manner and the F1 progeny along with the parents were subjected to AFLP analysis of chloroplast (cp) and nuclear DNA with seven and nine EcoRI / MseI primer combinations, respectively. cpDNA AFLP analysis identified 24 Pps-1 (DM-resistant)-specific unique fragments that were found to be maternally inherited in both the crosses, Pps-1 \u00d7 Jawahar-16 and Pps-1 \u00d7 H-9. In the case of nuclear DNA AFLP analysis, it was found that 17 fragments inherited from Pps-1 were common to the reciprocal crosses of both (i) Pps-1 and Jawahar-16 as well as (ii) Pps-1 and H-9. This is the first molecular investigation on the identification of polymorphism between DM-resistant and DM-susceptible opium poppy genotypes and development of DM-resistant opium poppy genotype-specific AFLP markers. These AFLP markers could be used in future genetic studies for analysis of linkage to the downy mildew resistance trait.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s12041-010-0015-0", 
        "inLanguage": "en", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1077130", 
            "issn": [
              "0022-1333", 
              "0973-7731"
            ], 
            "name": "Journal of Genetics", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "89"
          }
        ], 
        "keywords": [
          "downy mildew", 
          "opium poppy genotypes", 
          "AFLP analysis", 
          "AFLP markers", 
          "EcoRI/MseI primer combinations", 
          "opium poppy", 
          "fragment length polymorphism (AFLP) markers", 
          "first molecular investigation", 
          "length polymorphism markers", 
          "MseI primer combinations", 
          "identification of polymorphisms", 
          "resistance traits", 
          "susceptible genotypes", 
          "Peronospora arborescens", 
          "polymorphism markers", 
          "F1 progeny", 
          "sustainable way", 
          "primer combinations", 
          "reciprocal crosses", 
          "serious disease", 
          "future genetic studies", 
          "genotypes", 
          "cross", 
          "poppy", 
          "molecular investigations", 
          "mildew", 
          "cultivars", 
          "AFLP study", 
          "world-wide spread", 
          "analysis of linkage", 
          "genetic studies", 
          "traits", 
          "progeny", 
          "arborescens", 
          "markers", 
          "establishment", 
          "unique fragments", 
          "spread", 
          "identification", 
          "polymorphism", 
          "parents", 
          "disease", 
          "study", 
          "fragments", 
          "combination", 
          "chloroplasts", 
          "development", 
          "opium", 
          "analysis", 
          "linkage", 
          "DNA", 
          "nuclear DNA", 
          "results", 
          "PP-1", 
          "way", 
          "investigation", 
          "cases", 
          "manner", 
          "paper"
        ], 
        "name": "AFLP studies on downy-mildew-resistant and downy-mildew-susceptible genotypes of opium poppy", 
        "pagination": "9-19", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1002889547"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s12041-010-0015-0"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "20505242"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s12041-010-0015-0", 
          "https://app.dimensions.ai/details/publication/pub.1002889547"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-05-20T07:26", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_521.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s12041-010-0015-0"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12041-010-0015-0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12041-010-0015-0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12041-010-0015-0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12041-010-0015-0'


     

    This table displays all metadata directly associated to this object as RDF triples.

    274 TRIPLES      22 PREDICATES      116 URIs      90 LITERALS      20 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s12041-010-0015-0 schema:about N1396fee9a73045658246a039dd99c5d9
    2 N1e79ca462a2d4bd890590a14bed365c0
    3 N58f6a9f33ab343d0b60e1c52199db77b
    4 N609274ce8ff44c84979d24a7df701fa1
    5 N65560e71326d43918c232758920ad57f
    6 N6ec708575fe346f9815e7e26fa00b534
    7 N74f12c33b89049deae34ea73427788fb
    8 N888ac1c1a9c445f5844d54b3b40b1280
    9 Na31b216d95f1445893f0d0e1169842fb
    10 Nbb94e5b45970491da66b76be8741af4c
    11 Nd76d7bd92e18473c8c260953ea6f8893
    12 Nd8d1cadd1a4742ccb4c9cbe93cc21895
    13 Nedbd775de8864a18807d84020869319e
    14 anzsrc-for:06
    15 anzsrc-for:0604
    16 schema:author N846c740fbf0e47859f25b360a852d965
    17 schema:citation sg:pub.10.1007/978-1-4615-3276-7_1
    18 sg:pub.10.1007/978-1-4615-5419-6_13
    19 sg:pub.10.1007/bf00269301
    20 sg:pub.10.1007/bf00290238
    21 sg:pub.10.1007/bf00391760
    22 sg:pub.10.1007/bf01253966
    23 sg:pub.10.1007/bf02684905
    24 sg:pub.10.1007/s00122-002-1096-9
    25 sg:pub.10.1007/s00122-004-1774-x
    26 sg:pub.10.1007/s00122-004-1836-0
    27 sg:pub.10.1007/s002940050008
    28 sg:pub.10.1007/s00438-002-0713-2
    29 sg:pub.10.1007/s10681-008-9804-4
    30 sg:pub.10.1023/a:1003706931897
    31 sg:pub.10.1023/a:1007487806583
    32 sg:pub.10.1023/a:1007528101452
    33 sg:pub.10.1023/a:1009672008702
    34 sg:pub.10.1023/a:1017213630972
    35 schema:datePublished 2010-02-22
    36 schema:datePublishedReg 2010-02-22
    37 schema:description Downy mildew (DM) caused by Peronospora arborescens, is a serious disease in opium poppy (Papaver somniferum), which has a world-wide spread. The establishment of DM-resistant cultivars appears to be a sustainable way to control the disease. In this paper, we present the results of a study aimed at the identification of amplified fragment length polymorphism (AFLP) markers for DM-resistance in opium poppy. Three opium poppy genotypes (inbred over about 10 years): Pps-1 (DM-resistant), Jawahar-16 (DM-susceptible) and H-9 (DM-susceptible) were crossed in a diallel manner and the F1 progeny along with the parents were subjected to AFLP analysis of chloroplast (cp) and nuclear DNA with seven and nine EcoRI / MseI primer combinations, respectively. cpDNA AFLP analysis identified 24 Pps-1 (DM-resistant)-specific unique fragments that were found to be maternally inherited in both the crosses, Pps-1 × Jawahar-16 and Pps-1 × H-9. In the case of nuclear DNA AFLP analysis, it was found that 17 fragments inherited from Pps-1 were common to the reciprocal crosses of both (i) Pps-1 and Jawahar-16 as well as (ii) Pps-1 and H-9. This is the first molecular investigation on the identification of polymorphism between DM-resistant and DM-susceptible opium poppy genotypes and development of DM-resistant opium poppy genotype-specific AFLP markers. These AFLP markers could be used in future genetic studies for analysis of linkage to the downy mildew resistance trait.
    38 schema:genre article
    39 schema:inLanguage en
    40 schema:isAccessibleForFree false
    41 schema:isPartOf N11758e9ff2eb4b1791440c95f6181e82
    42 Na1033ddac234482daa0fe7c4234169e6
    43 sg:journal.1077130
    44 schema:keywords AFLP analysis
    45 AFLP markers
    46 AFLP study
    47 DNA
    48 EcoRI/MseI primer combinations
    49 F1 progeny
    50 MseI primer combinations
    51 PP-1
    52 Peronospora arborescens
    53 analysis
    54 analysis of linkage
    55 arborescens
    56 cases
    57 chloroplasts
    58 combination
    59 cross
    60 cultivars
    61 development
    62 disease
    63 downy mildew
    64 establishment
    65 first molecular investigation
    66 fragment length polymorphism (AFLP) markers
    67 fragments
    68 future genetic studies
    69 genetic studies
    70 genotypes
    71 identification
    72 identification of polymorphisms
    73 investigation
    74 length polymorphism markers
    75 linkage
    76 manner
    77 markers
    78 mildew
    79 molecular investigations
    80 nuclear DNA
    81 opium
    82 opium poppy
    83 opium poppy genotypes
    84 paper
    85 parents
    86 polymorphism
    87 polymorphism markers
    88 poppy
    89 primer combinations
    90 progeny
    91 reciprocal crosses
    92 resistance traits
    93 results
    94 serious disease
    95 spread
    96 study
    97 susceptible genotypes
    98 sustainable way
    99 traits
    100 unique fragments
    101 way
    102 world-wide spread
    103 schema:name AFLP studies on downy-mildew-resistant and downy-mildew-susceptible genotypes of opium poppy
    104 schema:pagination 9-19
    105 schema:productId N6bc24bece0ac497f987f0c25d4a8aa4d
    106 Na4fb5c494c13431b9493518d0f0eb0fa
    107 Nc122b394f8db4974a35d6480adb09656
    108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002889547
    109 https://doi.org/10.1007/s12041-010-0015-0
    110 schema:sdDatePublished 2022-05-20T07:26
    111 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    112 schema:sdPublisher N7ef1636e97e5487f9c557ba07dc84a9f
    113 schema:url https://doi.org/10.1007/s12041-010-0015-0
    114 sgo:license sg:explorer/license/
    115 sgo:sdDataset articles
    116 rdf:type schema:ScholarlyArticle
    117 N11758e9ff2eb4b1791440c95f6181e82 schema:volumeNumber 89
    118 rdf:type schema:PublicationVolume
    119 N1396fee9a73045658246a039dd99c5d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Genetic Markers
    121 rdf:type schema:DefinedTerm
    122 N1e79ca462a2d4bd890590a14bed365c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Peronospora
    124 rdf:type schema:DefinedTerm
    125 N3dba9678246c4e2a85189b4e84c40a7b rdf:first sg:person.010601233534.89
    126 rdf:rest rdf:nil
    127 N58f6a9f33ab343d0b60e1c52199db77b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Immunity, Innate
    129 rdf:type schema:DefinedTerm
    130 N609274ce8ff44c84979d24a7df701fa1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    131 schema:name Genotype
    132 rdf:type schema:DefinedTerm
    133 N65560e71326d43918c232758920ad57f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    134 schema:name Plant Diseases
    135 rdf:type schema:DefinedTerm
    136 N6bc24bece0ac497f987f0c25d4a8aa4d schema:name doi
    137 schema:value 10.1007/s12041-010-0015-0
    138 rdf:type schema:PropertyValue
    139 N6ec708575fe346f9815e7e26fa00b534 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Papaver
    141 rdf:type schema:DefinedTerm
    142 N74f12c33b89049deae34ea73427788fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Fatty Acids, Unsaturated
    144 rdf:type schema:DefinedTerm
    145 N7ef1636e97e5487f9c557ba07dc84a9f schema:name Springer Nature - SN SciGraph project
    146 rdf:type schema:Organization
    147 N846c740fbf0e47859f25b360a852d965 rdf:first sg:person.01346545715.70
    148 rdf:rest Naf99cfe37adb4a3b8e5872184b4319f9
    149 N858c649aaf3d40f38b9387d01968804e schema:affiliation grid-institutes:grid.417631.6
    150 schema:familyName Dhawan
    151 schema:givenName Omp.
    152 rdf:type schema:Person
    153 N888ac1c1a9c445f5844d54b3b40b1280 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    154 schema:name Hybridization, Genetic
    155 rdf:type schema:DefinedTerm
    156 N96d8f4172dd9422dbfe66c3e05f3c9c2 rdf:first N858c649aaf3d40f38b9387d01968804e
    157 rdf:rest Nb379300348e74301906197155632d419
    158 Na1033ddac234482daa0fe7c4234169e6 schema:issueNumber 1
    159 rdf:type schema:PublicationIssue
    160 Na31b216d95f1445893f0d0e1169842fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name Opium
    162 rdf:type schema:DefinedTerm
    163 Na4fb5c494c13431b9493518d0f0eb0fa schema:name pubmed_id
    164 schema:value 20505242
    165 rdf:type schema:PropertyValue
    166 Naf99cfe37adb4a3b8e5872184b4319f9 rdf:first sg:person.01207061437.96
    167 rdf:rest N96d8f4172dd9422dbfe66c3e05f3c9c2
    168 Nb379300348e74301906197155632d419 rdf:first sg:person.0746716663.99
    169 rdf:rest N3dba9678246c4e2a85189b4e84c40a7b
    170 Nbb94e5b45970491da66b76be8741af4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    171 schema:name Amplified Fragment Length Polymorphism Analysis
    172 rdf:type schema:DefinedTerm
    173 Nc122b394f8db4974a35d6480adb09656 schema:name dimensions_id
    174 schema:value pub.1002889547
    175 rdf:type schema:PropertyValue
    176 Nd76d7bd92e18473c8c260953ea6f8893 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name Inheritance Patterns
    178 rdf:type schema:DefinedTerm
    179 Nd8d1cadd1a4742ccb4c9cbe93cc21895 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    180 schema:name Crosses, Genetic
    181 rdf:type schema:DefinedTerm
    182 Nedbd775de8864a18807d84020869319e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    183 schema:name Cell Nucleus
    184 rdf:type schema:DefinedTerm
    185 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    186 schema:name Biological Sciences
    187 rdf:type schema:DefinedTerm
    188 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    189 schema:name Genetics
    190 rdf:type schema:DefinedTerm
    191 sg:journal.1077130 schema:issn 0022-1333
    192 0973-7731
    193 schema:name Journal of Genetics
    194 schema:publisher Springer Nature
    195 rdf:type schema:Periodical
    196 sg:person.010601233534.89 schema:affiliation grid-institutes:grid.417631.6
    197 schema:familyName Khanuja
    198 schema:givenName Suman P. S.
    199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010601233534.89
    200 rdf:type schema:Person
    201 sg:person.01207061437.96 schema:affiliation grid-institutes:grid.417631.6
    202 schema:familyName Shasany
    203 schema:givenName Ajit K.
    204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207061437.96
    205 rdf:type schema:Person
    206 sg:person.01346545715.70 schema:affiliation grid-institutes:grid.417631.6
    207 schema:familyName Dubey
    208 schema:givenName Mukesh K.
    209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346545715.70
    210 rdf:type schema:Person
    211 sg:person.0746716663.99 schema:affiliation grid-institutes:grid.417631.6
    212 schema:familyName Shukla
    213 schema:givenName Ashutosh K.
    214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746716663.99
    215 rdf:type schema:Person
    216 sg:pub.10.1007/978-1-4615-3276-7_1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022795456
    217 https://doi.org/10.1007/978-1-4615-3276-7_1
    218 rdf:type schema:CreativeWork
    219 sg:pub.10.1007/978-1-4615-5419-6_13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033981105
    220 https://doi.org/10.1007/978-1-4615-5419-6_13
    221 rdf:type schema:CreativeWork
    222 sg:pub.10.1007/bf00269301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005608283
    223 https://doi.org/10.1007/bf00269301
    224 rdf:type schema:CreativeWork
    225 sg:pub.10.1007/bf00290238 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001933298
    226 https://doi.org/10.1007/bf00290238
    227 rdf:type schema:CreativeWork
    228 sg:pub.10.1007/bf00391760 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035153226
    229 https://doi.org/10.1007/bf00391760
    230 rdf:type schema:CreativeWork
    231 sg:pub.10.1007/bf01253966 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023839901
    232 https://doi.org/10.1007/bf01253966
    233 rdf:type schema:CreativeWork
    234 sg:pub.10.1007/bf02684905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028727120
    235 https://doi.org/10.1007/bf02684905
    236 rdf:type schema:CreativeWork
    237 sg:pub.10.1007/s00122-002-1096-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052498237
    238 https://doi.org/10.1007/s00122-002-1096-9
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1007/s00122-004-1774-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030858521
    241 https://doi.org/10.1007/s00122-004-1774-x
    242 rdf:type schema:CreativeWork
    243 sg:pub.10.1007/s00122-004-1836-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035198430
    244 https://doi.org/10.1007/s00122-004-1836-0
    245 rdf:type schema:CreativeWork
    246 sg:pub.10.1007/s002940050008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048144039
    247 https://doi.org/10.1007/s002940050008
    248 rdf:type schema:CreativeWork
    249 sg:pub.10.1007/s00438-002-0713-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001077092
    250 https://doi.org/10.1007/s00438-002-0713-2
    251 rdf:type schema:CreativeWork
    252 sg:pub.10.1007/s10681-008-9804-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012941598
    253 https://doi.org/10.1007/s10681-008-9804-4
    254 rdf:type schema:CreativeWork
    255 sg:pub.10.1023/a:1003706931897 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027333886
    256 https://doi.org/10.1023/a:1003706931897
    257 rdf:type schema:CreativeWork
    258 sg:pub.10.1023/a:1007487806583 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033208159
    259 https://doi.org/10.1023/a:1007487806583
    260 rdf:type schema:CreativeWork
    261 sg:pub.10.1023/a:1007528101452 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020688090
    262 https://doi.org/10.1023/a:1007528101452
    263 rdf:type schema:CreativeWork
    264 sg:pub.10.1023/a:1009672008702 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021074442
    265 https://doi.org/10.1023/a:1009672008702
    266 rdf:type schema:CreativeWork
    267 sg:pub.10.1023/a:1017213630972 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016322897
    268 https://doi.org/10.1023/a:1017213630972
    269 rdf:type schema:CreativeWork
    270 grid-institutes:grid.417631.6 schema:alternateName Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India
    271 Genetics and Plant Breeding Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India
    272 schema:name Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India
    273 Genetics and Plant Breeding Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226 015, Lucknow, India
    274 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...