RNA interference for the control of whiteflies (Bemisia tabaci) by oral route View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-03

AUTHORS

Santosh Kumar Upadhyay, K Chandrashekar, Nidhi Thakur, Praveen Chandra Verma, J Francis Borgio, Pradhyumna Kumar Singh, Rakesh Tuli

ABSTRACT

RNA interference (RNAi)-mediated gene silencing was explored for the control of sap-sucking pest Bemisia tabaci, commonly known as whitefly. dsRNAs and siRNAs were synthesized from five different genes – actin ortholog, ADP/ATP translocase, α-tubulin, ribosomal protein L9 (RPL9) and V-ATPase A subunit. A simplified insect bioassay method was developed for the delivery of ds/siRNA through the oral route, and efficacy was evaluated. ds/siRNA caused 29–97% mortality after 6 days of feeding. Each insect ingested nearly 150 nl of insect diet per day, which contained a maximum of 6 ng of RNA. Knocking down the expression of RPL9 and V-ATPase A caused higher mortality with LC50 11.21 and 3.08 μg/ml, respectively, as compared to other genes. Semi-quantitative PCR of the treated insects showed significant decrease in the level of RPL9 and V-ATPase A transcripts. siRNAs were found stable in the insect diet for at least 7 days at the room temperature. Phloem-specific expression of dsRNAs of RPL9 and V-ATPase A in transgenic plants for the protection against whiteflies might be an interesting application of this technology. More... »

PAGES

153-161

Journal

TITLE

Journal of Biosciences

ISSUE

1

VOLUME

36

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12038-011-9009-1

DOI

http://dx.doi.org/10.1007/s12038-011-9009-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015221730

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21451256


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