Inactivation of a transgene due to transposition of insertion sequence (IS136) of Agrobacterium tumefaciens View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-06

AUTHORS

Preeti Rawat, Sanjeev Kumar, Deepak Pental, Pradeep Kumar Burma

ABSTRACT

Agrobacterium strains harbour insertion sequences, which are known to transpose into genomes as well as into Ti plasmids. In this study we report the inactivation of a transgene due to transposition of the A. tumefaciens insertion sequence IS136. The transposition was discovered following transformation of plant tissues, although the fidelity of the binary vector was confirmed following transformation into Agrobacterium. Such transpositions are rare but can occur and it is thus important to check the fidelity of the binary vector at different times of Agrobacterium growth in order to avoid failure in achieving transgene expression. More... »

PAGES

199-202

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12038-009-0023-5

DOI

http://dx.doi.org/10.1007/s12038-009-0023-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013402836

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19550035


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