Extended Culture of Encapsulated Human Blastocysts in Alginate Hydrogel Containing Decidualized Endometrial Stromal Cells in the Presence of Melatonin View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-10

AUTHORS

Fatemeh Arjmand, Manijeh Khanmohammadi, Shaghayegh Arasteh, Afsaneh Mohammadzadeh, Somaieh Kazemnejad, Mohammad-Mehdi Akhondi

ABSTRACT

Extended in vitro culture of human embryos beyond blastocyst stage could serve as a tool to explore the molecular and physiological mechanisms underlying embryo development and to identify factors regulating pregnancy outcomes. This study presents the first report on the maintenance of human embryo in vitro by alginate co-encapsulation of human blastocyst and decidualized endometrial stromal cells (EnSCs) under melatonin-fortified culture conditions. The effectiveness of the 3D culture system was studied through monitoring of embryo development in terms of survival time, viability, morphological changes, and production of the two hormones of 17b-oestradiol and human chorionic gonadotropin. The embryo structural integrity was preserved during alginate encapsulation; however, only 23 % of the encapsulated embryos could retain in the hydrogels over time and survived until day 4 post-encapsulation. The culture medium fortification with melatonin significantly elevated the maintenance rate of expanded embryos in alginate beads by 65 % and prolonged survival time of human embryos to day 5. Furthermore, embryo co-culture with EnSCs using melatonin-fortified medium increased the survival time of encapsulated embryos to 44 %. The levels of two measured hormones significantly rose at day 4 in comparison with day 2 post-encapsulation especially in the group co-encapsulated with EnSCs and cultivated in melatonin-fortified culture medium. These data are the first evidence representing in vitro development of human embryos until day 10 post-fertilization. This achievement can facilitate the investigation of the mechanisms regulating human embryo development. More... »

PAGES

684-694

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12033-016-9968-4

DOI

http://dx.doi.org/10.1007/s12033-016-9968-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051588060

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27514657


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1114", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Paediatrics and Reproductive Medicine", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adult", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alginates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blastocyst", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Coculture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryo Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryonic Development", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endometrium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucuronic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hexuronic Acids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogel, Polyethylene Glycol Dimethacrylate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Melatonin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pregnancy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stromal Cells", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Academic Center for Education, Culture and Research", 
          "id": "https://www.grid.ac/institutes/grid.417689.5", 
          "name": [
            "Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, P.O. Box: 1177-19615, Tehran, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Arjmand", 
        "givenName": "Fatemeh", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Academic Center for Education, Culture and Research", 
          "id": "https://www.grid.ac/institutes/grid.417689.5", 
          "name": [
            "Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, P.O. Box: 1177-19615, Tehran, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Khanmohammadi", 
        "givenName": "Manijeh", 
        "id": "sg:person.01235511471.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235511471.73"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Academic Center for Education, Culture and Research", 
          "id": "https://www.grid.ac/institutes/grid.417689.5", 
          "name": [
            "Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, P.O. Box: 1177-19615, Tehran, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Arasteh", 
        "givenName": "Shaghayegh", 
        "id": "sg:person.01026002725.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026002725.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Academic Center for Education, Culture and Research", 
          "id": "https://www.grid.ac/institutes/grid.417689.5", 
          "name": [
            "Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, P.O. Box: 1177-19615, Tehran, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mohammadzadeh", 
        "givenName": "Afsaneh", 
        "id": "sg:person.01170560766.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170560766.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Academic Center for Education, Culture and Research", 
          "id": "https://www.grid.ac/institutes/grid.417689.5", 
          "name": [
            "Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, P.O. Box: 1177-19615, Tehran, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kazemnejad", 
        "givenName": "Somaieh", 
        "id": "sg:person.0641532123.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641532123.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Academic Center for Education, Culture and Research", 
          "id": "https://www.grid.ac/institutes/grid.417689.5", 
          "name": [
            "Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, P.O. Box: 1177-19615, Tehran, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Akhondi", 
        "givenName": "Mohammad-Mehdi", 
        "id": "sg:person.015610621747.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015610621747.52"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.theriogenology.2004.01.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001721839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.addr.2012.09.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002933541"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.theriogenology.2005.09.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005632149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1034/j.1600-079x.2000.280107.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006724137"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/deg072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007076790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1472-6483(10)61960-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010420843"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0142-9612(00)00201-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011223982"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0142-9612(98)00107-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013500192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-079x.2011.00916.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014528261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biomaterials.2005.06.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018183039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biomaterials.2005.06.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018183039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/deh750", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021087428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0015-0282(97)81382-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021456320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humupd/dmr006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021778497"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.theriogenology.2015.03.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024518281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1530/joe-14-0430", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025866094"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1071/rd13008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025922207"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/ma6041285", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030743945"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/den420", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030934621"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod.104.033480", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031785028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0806219105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033458679"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0093641", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033514894"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0119463", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033962553"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/jpi.12126", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038237906"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod.109.080630", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039689515"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0290(19991205)65:5<605::aid-bit14>3.0.co;2-c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039771163"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod27.4.925", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040664935"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod.103.025916", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042232525"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1439-0442.2002.00463.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042771024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0041424", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047327075"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0015-0282(16)60926-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050577956"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0967199411000086", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053954366"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/00220345010800111501", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063628609"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/00220345010800111501", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063628609"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079252817", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.humrep.a136971", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079346599"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-10", 
    "datePublishedReg": "2016-10-01", 
    "description": "Extended in vitro culture of human embryos beyond blastocyst stage could serve as a tool to explore the molecular and physiological mechanisms underlying embryo development and to identify factors regulating pregnancy outcomes. This study presents the first report on the maintenance of human embryo in vitro by alginate co-encapsulation of human blastocyst and decidualized endometrial stromal cells (EnSCs) under melatonin-fortified culture conditions. The effectiveness of the 3D culture system was studied through monitoring of embryo development in terms of survival time, viability, morphological changes, and production of the two hormones of 17b-oestradiol and human chorionic gonadotropin. The embryo structural integrity was preserved during alginate encapsulation; however, only 23\u00a0% of the encapsulated embryos could retain in the hydrogels over time and survived until day 4 post-encapsulation. The culture medium fortification with melatonin significantly elevated the maintenance rate of expanded embryos in alginate beads by 65\u00a0% and prolonged survival time of human embryos to day 5. Furthermore, embryo co-culture with EnSCs using melatonin-fortified medium increased the survival time of encapsulated embryos to 44\u00a0%. The levels of two measured hormones significantly rose at day 4 in comparison with day 2 post-encapsulation especially in the group co-encapsulated with EnSCs and cultivated in melatonin-fortified culture medium. These data are the first evidence representing in vitro development of human embryos until day 10 post-fertilization. This achievement can facilitate the investigation of the mechanisms regulating human embryo development. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s12033-016-9968-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1106201", 
        "issn": [
          "1073-6085", 
          "1559-0305"
        ], 
        "name": "Molecular Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "58"
      }
    ], 
    "name": "Extended Culture of Encapsulated Human Blastocysts in Alginate Hydrogel Containing Decidualized Endometrial Stromal Cells in the Presence of Melatonin", 
    "pagination": "684-694", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "f5bd96d95f02f39bec183c94b244ca2690955c5e7c3d23ae1c9897e2f4500635"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27514657"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9423533"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s12033-016-9968-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1051588060"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s12033-016-9968-4", 
      "https://app.dimensions.ai/details/publication/pub.1051588060"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:38", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000363_0000000363/records_70040_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs12033-016-9968-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12033-016-9968-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12033-016-9968-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12033-016-9968-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12033-016-9968-4'


 

This table displays all metadata directly associated to this object as RDF triples.

268 TRIPLES      21 PREDICATES      79 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s12033-016-9968-4 schema:about N12b6b49f13204d4aa48b70a2a3601f9e
2 N29e2d12f3a8848b2bcde34d44bcf75f0
3 N3e20629a1dd14239a86da8adb4dd647f
4 N4264c59406004b20859e1e8a3a2296d1
5 N44ee1f499df6492eb6666f3f7fdec116
6 N5d19fbc845d14707853d5b7e66e3e177
7 N7f3bb3a5afbb4e8e8eab2282bf7bdce4
8 N84f4a86ba0144b0a880abd691e54b20e
9 N9d934c02ad9045488b8d86246aa05cb7
10 Na87a5694dc364476afb9c4ff3791b200
11 Nb3567feca2764e0192f7f038bceea2c2
12 Nca51f01306d04813924bcf1457009678
13 Nccc9d165559f4b789f87e140ec7014a9
14 Nd6ed36cc5ccb4fae9648a23ed264cb64
15 Nf7a5dd8186fe47e39f4b8eafcc685868
16 Nff35e8b47f2a4332b3a5c46183ee9813
17 anzsrc-for:11
18 anzsrc-for:1114
19 schema:author N2fd18b279e98478fb8de273aacd25ec4
20 schema:citation https://app.dimensions.ai/details/publication/pub.1079252817
21 https://doi.org/10.1002/(sici)1097-0290(19991205)65:5<605::aid-bit14>3.0.co;2-c
22 https://doi.org/10.1016/j.addr.2012.09.007
23 https://doi.org/10.1016/j.biomaterials.2005.06.016
24 https://doi.org/10.1016/j.theriogenology.2004.01.007
25 https://doi.org/10.1016/j.theriogenology.2005.09.021
26 https://doi.org/10.1016/j.theriogenology.2015.03.011
27 https://doi.org/10.1016/s0015-0282(16)60926-3
28 https://doi.org/10.1016/s0015-0282(97)81382-9
29 https://doi.org/10.1016/s0142-9612(00)00201-5
30 https://doi.org/10.1016/s0142-9612(98)00107-0
31 https://doi.org/10.1016/s1472-6483(10)61960-8
32 https://doi.org/10.1017/s0967199411000086
33 https://doi.org/10.1034/j.1600-079x.2000.280107.x
34 https://doi.org/10.1046/j.1439-0442.2002.00463.x
35 https://doi.org/10.1071/rd13008
36 https://doi.org/10.1073/pnas.0806219105
37 https://doi.org/10.1093/humrep/deg072
38 https://doi.org/10.1093/humrep/deh750
39 https://doi.org/10.1093/humrep/den420
40 https://doi.org/10.1093/humupd/dmr006
41 https://doi.org/10.1093/oxfordjournals.humrep.a136971
42 https://doi.org/10.1095/biolreprod.103.025916
43 https://doi.org/10.1095/biolreprod.104.033480
44 https://doi.org/10.1095/biolreprod.109.080630
45 https://doi.org/10.1095/biolreprod27.4.925
46 https://doi.org/10.1111/j.1600-079x.2011.00916.x
47 https://doi.org/10.1111/jpi.12126
48 https://doi.org/10.1177/00220345010800111501
49 https://doi.org/10.1371/journal.pone.0041424
50 https://doi.org/10.1371/journal.pone.0093641
51 https://doi.org/10.1371/journal.pone.0119463
52 https://doi.org/10.1530/joe-14-0430
53 https://doi.org/10.3390/ma6041285
54 schema:datePublished 2016-10
55 schema:datePublishedReg 2016-10-01
56 schema:description Extended in vitro culture of human embryos beyond blastocyst stage could serve as a tool to explore the molecular and physiological mechanisms underlying embryo development and to identify factors regulating pregnancy outcomes. This study presents the first report on the maintenance of human embryo in vitro by alginate co-encapsulation of human blastocyst and decidualized endometrial stromal cells (EnSCs) under melatonin-fortified culture conditions. The effectiveness of the 3D culture system was studied through monitoring of embryo development in terms of survival time, viability, morphological changes, and production of the two hormones of 17b-oestradiol and human chorionic gonadotropin. The embryo structural integrity was preserved during alginate encapsulation; however, only 23 % of the encapsulated embryos could retain in the hydrogels over time and survived until day 4 post-encapsulation. The culture medium fortification with melatonin significantly elevated the maintenance rate of expanded embryos in alginate beads by 65 % and prolonged survival time of human embryos to day 5. Furthermore, embryo co-culture with EnSCs using melatonin-fortified medium increased the survival time of encapsulated embryos to 44 %. The levels of two measured hormones significantly rose at day 4 in comparison with day 2 post-encapsulation especially in the group co-encapsulated with EnSCs and cultivated in melatonin-fortified culture medium. These data are the first evidence representing in vitro development of human embryos until day 10 post-fertilization. This achievement can facilitate the investigation of the mechanisms regulating human embryo development.
57 schema:genre research_article
58 schema:inLanguage en
59 schema:isAccessibleForFree false
60 schema:isPartOf N9ee794eb08084e198fde11f7e18ef83e
61 Nf7855ea67917407aa3dac7661eb4170a
62 sg:journal.1106201
63 schema:name Extended Culture of Encapsulated Human Blastocysts in Alginate Hydrogel Containing Decidualized Endometrial Stromal Cells in the Presence of Melatonin
64 schema:pagination 684-694
65 schema:productId N30da9e54ef64403680316bc64321dcdf
66 N5d89fb867e104e4787c267fe47014495
67 Na8fd23c02ada4824a0b3525750ecf5bc
68 Nada16a19172645caa680d6af757e827e
69 Ncb307c19be2e4d6aad6f0cd9da1943f7
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051588060
71 https://doi.org/10.1007/s12033-016-9968-4
72 schema:sdDatePublished 2019-04-11T12:38
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher Nb7403b977679455abf411612f442baae
75 schema:url https://link.springer.com/10.1007%2Fs12033-016-9968-4
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N12b6b49f13204d4aa48b70a2a3601f9e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Stromal Cells
81 rdf:type schema:DefinedTerm
82 N186d222fec9b47c1b33f7dfe4112df12 rdf:first sg:person.01026002725.63
83 rdf:rest N55d7e1ea2ef8477ab44ba5d1e86e3f3e
84 N29e2d12f3a8848b2bcde34d44bcf75f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Endometrium
86 rdf:type schema:DefinedTerm
87 N2fd18b279e98478fb8de273aacd25ec4 rdf:first N4dddcfb7f22a487e914d4386f95864a6
88 rdf:rest Nd0eb3ab6f11a445f9cf0b33c809a6477
89 N30da9e54ef64403680316bc64321dcdf schema:name readcube_id
90 schema:value f5bd96d95f02f39bec183c94b244ca2690955c5e7c3d23ae1c9897e2f4500635
91 rdf:type schema:PropertyValue
92 N3e20629a1dd14239a86da8adb4dd647f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Hexuronic Acids
94 rdf:type schema:DefinedTerm
95 N4264c59406004b20859e1e8a3a2296d1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Embryo Culture Techniques
97 rdf:type schema:DefinedTerm
98 N44ee1f499df6492eb6666f3f7fdec116 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Alginates
100 rdf:type schema:DefinedTerm
101 N4dddcfb7f22a487e914d4386f95864a6 schema:affiliation https://www.grid.ac/institutes/grid.417689.5
102 schema:familyName Arjmand
103 schema:givenName Fatemeh
104 rdf:type schema:Person
105 N55d7e1ea2ef8477ab44ba5d1e86e3f3e rdf:first sg:person.01170560766.30
106 rdf:rest Nab61c5a7ef0b4f819a2706d5e4b846c4
107 N5d19fbc845d14707853d5b7e66e3e177 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Blastocyst
109 rdf:type schema:DefinedTerm
110 N5d89fb867e104e4787c267fe47014495 schema:name pubmed_id
111 schema:value 27514657
112 rdf:type schema:PropertyValue
113 N7f3bb3a5afbb4e8e8eab2282bf7bdce4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Female
115 rdf:type schema:DefinedTerm
116 N84f4a86ba0144b0a880abd691e54b20e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Coculture Techniques
118 rdf:type schema:DefinedTerm
119 N9d934c02ad9045488b8d86246aa05cb7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Hydrogel, Polyethylene Glycol Dimethacrylate
121 rdf:type schema:DefinedTerm
122 N9ee794eb08084e198fde11f7e18ef83e schema:issueNumber 10
123 rdf:type schema:PublicationIssue
124 Na63e274ad79d4ad7aa56df2b8e55b736 rdf:first sg:person.015610621747.52
125 rdf:rest rdf:nil
126 Na87a5694dc364476afb9c4ff3791b200 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Pregnancy
128 rdf:type schema:DefinedTerm
129 Na8fd23c02ada4824a0b3525750ecf5bc schema:name dimensions_id
130 schema:value pub.1051588060
131 rdf:type schema:PropertyValue
132 Nab61c5a7ef0b4f819a2706d5e4b846c4 rdf:first sg:person.0641532123.05
133 rdf:rest Na63e274ad79d4ad7aa56df2b8e55b736
134 Nada16a19172645caa680d6af757e827e schema:name nlm_unique_id
135 schema:value 9423533
136 rdf:type schema:PropertyValue
137 Nb3567feca2764e0192f7f038bceea2c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Embryonic Development
139 rdf:type schema:DefinedTerm
140 Nb7403b977679455abf411612f442baae schema:name Springer Nature - SN SciGraph project
141 rdf:type schema:Organization
142 Nca51f01306d04813924bcf1457009678 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Humans
144 rdf:type schema:DefinedTerm
145 Ncb307c19be2e4d6aad6f0cd9da1943f7 schema:name doi
146 schema:value 10.1007/s12033-016-9968-4
147 rdf:type schema:PropertyValue
148 Nccc9d165559f4b789f87e140ec7014a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Glucuronic Acid
150 rdf:type schema:DefinedTerm
151 Nd0eb3ab6f11a445f9cf0b33c809a6477 rdf:first sg:person.01235511471.73
152 rdf:rest N186d222fec9b47c1b33f7dfe4112df12
153 Nd6ed36cc5ccb4fae9648a23ed264cb64 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Melatonin
155 rdf:type schema:DefinedTerm
156 Nf7855ea67917407aa3dac7661eb4170a schema:volumeNumber 58
157 rdf:type schema:PublicationVolume
158 Nf7a5dd8186fe47e39f4b8eafcc685868 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Culture Media
160 rdf:type schema:DefinedTerm
161 Nff35e8b47f2a4332b3a5c46183ee9813 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Adult
163 rdf:type schema:DefinedTerm
164 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
165 schema:name Medical and Health Sciences
166 rdf:type schema:DefinedTerm
167 anzsrc-for:1114 schema:inDefinedTermSet anzsrc-for:
168 schema:name Paediatrics and Reproductive Medicine
169 rdf:type schema:DefinedTerm
170 sg:journal.1106201 schema:issn 1073-6085
171 1559-0305
172 schema:name Molecular Biotechnology
173 rdf:type schema:Periodical
174 sg:person.01026002725.63 schema:affiliation https://www.grid.ac/institutes/grid.417689.5
175 schema:familyName Arasteh
176 schema:givenName Shaghayegh
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026002725.63
178 rdf:type schema:Person
179 sg:person.01170560766.30 schema:affiliation https://www.grid.ac/institutes/grid.417689.5
180 schema:familyName Mohammadzadeh
181 schema:givenName Afsaneh
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170560766.30
183 rdf:type schema:Person
184 sg:person.01235511471.73 schema:affiliation https://www.grid.ac/institutes/grid.417689.5
185 schema:familyName Khanmohammadi
186 schema:givenName Manijeh
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235511471.73
188 rdf:type schema:Person
189 sg:person.015610621747.52 schema:affiliation https://www.grid.ac/institutes/grid.417689.5
190 schema:familyName Akhondi
191 schema:givenName Mohammad-Mehdi
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015610621747.52
193 rdf:type schema:Person
194 sg:person.0641532123.05 schema:affiliation https://www.grid.ac/institutes/grid.417689.5
195 schema:familyName Kazemnejad
196 schema:givenName Somaieh
197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641532123.05
198 rdf:type schema:Person
199 https://app.dimensions.ai/details/publication/pub.1079252817 schema:CreativeWork
200 https://doi.org/10.1002/(sici)1097-0290(19991205)65:5<605::aid-bit14>3.0.co;2-c schema:sameAs https://app.dimensions.ai/details/publication/pub.1039771163
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/j.addr.2012.09.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002933541
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/j.biomaterials.2005.06.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018183039
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/j.theriogenology.2004.01.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001721839
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/j.theriogenology.2005.09.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005632149
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/j.theriogenology.2015.03.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024518281
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/s0015-0282(16)60926-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050577956
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/s0015-0282(97)81382-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021456320
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/s0142-9612(00)00201-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011223982
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/s0142-9612(98)00107-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013500192
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/s1472-6483(10)61960-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010420843
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1017/s0967199411000086 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053954366
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1034/j.1600-079x.2000.280107.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006724137
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1046/j.1439-0442.2002.00463.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1042771024
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1071/rd13008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025922207
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1073/pnas.0806219105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033458679
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1093/humrep/deg072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007076790
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1093/humrep/deh750 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021087428
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1093/humrep/den420 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030934621
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1093/humupd/dmr006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021778497
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1093/oxfordjournals.humrep.a136971 schema:sameAs https://app.dimensions.ai/details/publication/pub.1079346599
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1095/biolreprod.103.025916 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042232525
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1095/biolreprod.104.033480 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031785028
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1095/biolreprod.109.080630 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039689515
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1095/biolreprod27.4.925 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040664935
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1111/j.1600-079x.2011.00916.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014528261
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1111/jpi.12126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038237906
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1177/00220345010800111501 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063628609
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1371/journal.pone.0041424 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047327075
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1371/journal.pone.0093641 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033514894
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1371/journal.pone.0119463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033962553
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1530/joe-14-0430 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025866094
263 rdf:type schema:CreativeWork
264 https://doi.org/10.3390/ma6041285 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030743945
265 rdf:type schema:CreativeWork
266 https://www.grid.ac/institutes/grid.417689.5 schema:alternateName Academic Center for Education, Culture and Research
267 schema:name Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, P.O. Box: 1177-19615, Tehran, Iran
268 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...