Quantitative PCR Pitfalls: The Case of the Human Placenta View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-11

AUTHORS

Dave Lanoix, Andrée-Anne Lacasse, Joey St-Pierre, Sean C. Taylor, Maude Ethier-Chiasson, Julie Lafond, Cathy Vaillancourt

ABSTRACT

Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) is a rapid and high throughput gene expression quantification technology. In order to obtain accurate results, several key experimental design and standardization steps must be rigorously followed as previously described in the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines. This study investigates the effect of reference gene normalization and the impact of RNA degradation on gene expression of 8-oxoguanine DNA glycosylase in human placenta from pregnancies complicated by preeclampsia and gestational diabetes mellitus and their gestation-matched controls. The data presented here show how RNA quality and appropriate reference gene selection is not only important to obtain accurate and reproducible RT-qPCR data but how different and even opposite results can be reported if the key steps outlined in the MIQE guidelines are not followed. The procedures and associated results presented in this study provide the first practical application of the MIQE guidelines to placental analysis in normal and pathological pregnancies. More... »

PAGES

234-243

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12033-012-9539-2

DOI

http://dx.doi.org/10.1007/s12033-012-9539-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024232235

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22528553


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Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12033-012-9539-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12033-012-9539-2'


 

This table displays all metadata directly associated to this object as RDF triples.

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